##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734165
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.078451029400746	31.0	31.0	34.0	28.0	34.0
2	31.386907575272588	31.0	31.0	34.0	28.0	34.0
3	31.540917913548043	31.0	31.0	34.0	30.0	34.0
4	35.50754939284766	37.0	35.0	37.0	33.0	37.0
5	35.090367969053275	37.0	35.0	37.0	32.0	37.0
6	35.25265301396825	37.0	35.0	37.0	32.0	37.0
7	35.1645951523159	37.0	35.0	37.0	32.0	37.0
8	35.55802033602801	37.0	35.0	37.0	35.0	37.0
9	36.95304189112802	39.0	37.0	39.0	33.0	39.0
10	36.22786839470691	37.0	35.0	39.0	32.0	39.0
11	36.43976081671014	38.0	35.0	39.0	32.0	39.0
12	36.05557061423522	37.0	35.0	39.0	32.0	39.0
13	35.63035012565295	37.0	35.0	39.0	30.0	39.0
14	36.78448305217492	39.0	35.0	40.0	31.0	41.0
15	36.81230785994974	38.0	35.0	40.0	31.0	41.0
16	36.76967711617961	38.0	35.0	40.0	32.0	41.0
17	36.73446568550666	38.0	35.0	40.0	32.0	41.0
18	36.542021207766645	38.0	35.0	40.0	31.0	41.0
19	36.690115982102114	38.0	35.0	40.0	31.0	41.0
20	36.47707395476494	38.0	35.0	40.0	31.0	41.0
21	36.565503667431706	38.0	35.0	40.0	31.0	41.0
22	36.670375188138905	38.0	35.0	40.0	31.0	41.0
23	36.47395340284541	38.0	35.0	40.0	31.0	41.0
24	36.08908079246491	38.0	34.0	40.0	30.0	41.0
25	36.25018490393849	38.0	35.0	40.0	31.0	41.0
26	36.22978077135249	38.0	35.0	40.0	31.0	41.0
27	36.119486763874605	38.0	35.0	40.0	30.0	41.0
28	36.1326418448169	38.0	35.0	40.0	30.0	41.0
29	35.89434936288164	38.0	34.0	40.0	30.0	41.0
30	35.92618280631738	38.0	35.0	40.0	30.0	41.0
31	35.81502523274741	38.0	34.0	40.0	30.0	41.0
32	35.804018170302314	38.0	34.0	40.0	30.0	41.0
33	35.7769697547554	38.0	35.0	40.0	30.0	41.0
34	35.70682339800999	38.0	35.0	40.0	30.0	41.0
35	35.53575422418666	38.0	34.0	40.0	29.0	41.0
36	35.45339126763057	38.0	34.0	40.0	29.0	41.0
37	35.30027582355465	37.0	34.0	40.0	28.0	41.0
38	35.08875661465747	37.0	34.0	40.0	27.0	41.0
39	35.08136726757609	37.0	34.0	40.0	27.0	41.0
40	34.90000068104582	37.0	34.0	40.0	26.0	41.0
41	34.93607022944433	37.0	34.0	40.0	26.0	41.0
42	34.87480607220448	37.0	34.0	40.0	26.0	41.0
43	34.87022944433472	37.0	34.0	40.0	27.0	41.0
44	34.73721030013689	37.0	34.0	40.0	26.0	41.0
45	34.4911402750063	36.0	33.0	40.0	25.0	41.0
46	33.44517104465617	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	6.0
11	9.0
12	18.0
13	15.0
14	22.0
15	44.0
16	86.0
17	172.0
18	385.0
19	665.0
20	1190.0
21	2125.0
22	2871.0
23	4167.0
24	5473.0
25	6720.0
26	7808.0
27	9210.0
28	10851.0
29	13421.0
30	17641.0
31	23329.0
32	31633.0
33	44979.0
34	75140.0
35	81994.0
36	69973.0
37	95607.0
38	136152.0
39	92448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.335667050322478	35.001259934755815	18.614889023584617	25.048183991337098
2	22.40572623320371	35.19958047577861	19.026921741025518	23.367771549992167
3	21.042544931997575	32.62018756001716	20.792601118277226	25.544666389708038
4	20.12790040386017	34.392132558757226	18.65575177242173	26.82421526496087
5	17.78728215047026	38.02401367539994	17.5757493206568	26.612954853472992
6	19.00281271921162	39.670782453535644	19.569034208931235	21.757370618321495
7	79.63237146962877	12.2656351092738	4.371769288920066	3.730224132177372
8	82.01535077264649	9.888785218581654	3.4687025396198403	4.62716146915203
9	77.60408082651719	12.90105085369093	5.551613057010345	3.9432552627815274
10	40.2592060367901	35.83758419428875	10.914985051044383	12.988224717876772
11	33.26622761913194	28.94185911886293	21.13012742367179	16.661785838333344
12	31.092601799323038	28.198974345004192	25.519603903754607	15.188819951918164
13	23.26084735720172	36.27222763275286	21.753420552600574	18.713504457444852
14	18.168531597120538	37.31368289144811	27.21254758807625	17.305237923355104
15	17.171889152983322	31.373056465508437	32.2855216470412	19.169532734467047
16	19.761906383442415	29.405515109001385	31.446473204252452	19.38610530330375
17	19.591781139117227	28.42440050942227	24.901078095523484	27.082740255937015
18	22.43936989641293	29.93101005904667	25.311340093848113	22.318279950692283
19	23.11823636376019	32.86509163471427	23.410677436271136	20.605994565254406
20	24.730135596221558	31.974147500902383	23.002594784551157	20.2931221183249
21	21.837052978553867	31.73918669508898	24.900397049709532	21.52336327664762
22	21.79060565404235	29.57577656248936	24.51601479231508	24.117602991153213
23	21.30869763608998	32.62018756001716	22.7598700564587	23.31124474743416
24	19.331894056513182	32.236622557599446	27.22358053026227	21.2079028556251
25	19.326445690001567	31.441297256066413	26.728187805193652	22.50406924873836
26	20.44771951809198	35.291113033173744	22.50379683041278	21.757370618321495
27	17.964762689586127	34.212200254711135	25.608003650405564	22.215033405297174
28	18.195773429678614	33.04761191285338	29.899409533279304	18.857205124188702
29	18.78065557470051	31.16792546634612	27.83815627277247	22.213262686180897
30	22.28109484925051	31.898687624716516	26.010774144776722	19.809443381256255
31	25.467572003568677	31.1285610182997	21.925452725204824	21.478414252926793
32	23.94025866120014	29.660634870907764	24.46575361124543	21.933352856646668
33	22.780710058365628	30.998208849509307	24.779579522314464	21.4415015698106
34	21.122091083067158	31.101727813229996	25.526141943568543	22.250039160134303
35	21.54842576260105	30.392078075092112	26.138265921148516	21.921230241158323
36	25.533497238359224	30.225085641511107	24.730135596221558	19.511281523908114
37	22.765454632133103	31.111943500439278	24.855584235151497	21.267017632276122
38	21.686814271996077	32.107223852948586	25.487730959661658	20.71823091539368
39	22.814626139900433	29.28646830072259	26.26208005012497	21.63682550925201
40	23.339167625806187	29.132960574257833	24.42420981659436	23.10366198334162
41	20.512555079580203	27.497769574959307	26.946394883983842	25.04328046147664
42	24.01176847166509	27.557429188261494	23.94434493608385	24.48645740398957
43	23.02833831631854	27.813774832632994	25.20577799268557	23.9521088583629
44	22.640142202365954	29.250917709234297	25.597243126545123	22.511696961854625
45	22.002955738832554	32.58354729522655	23.788113026363284	21.625383939577617
46	21.044315651113852	30.404881736394408	28.34975788821314	20.201044724278603
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23806.0
1	18524.5
2	13243.0
3	8488.0
4	3733.0
5	3224.5
6	2716.0
7	2716.0
8	2726.5
9	2737.0
10	2677.5
11	2618.0
12	2647.5
13	2677.0
14	2730.5
15	2784.0
16	2819.0
17	2854.0
18	3105.5
19	3357.0
20	3357.0
21	3059.0
22	2761.0
23	2737.5
24	2714.0
25	4103.5
26	5493.0
27	4979.5
28	4466.0
29	6222.0
30	7978.0
31	7978.0
32	10805.5
33	13633.0
34	15396.0
35	17159.0
36	20540.0
37	23921.0
38	25273.0
39	26625.0
40	31836.5
41	37048.0
42	40179.0
43	43310.0
44	43310.0
45	52005.0
46	60700.0
47	65499.5
48	70299.0
49	65935.5
50	61572.0
51	57453.0
52	53334.0
53	48635.5
54	43937.0
55	41031.0
56	38125.0
57	38125.0
58	36064.0
59	34003.0
60	32777.0
61	31551.0
62	29724.5
63	27898.0
64	24592.0
65	21286.0
66	18584.0
67	15882.0
68	13373.0
69	10864.0
70	10864.0
71	9334.5
72	7805.0
73	6295.0
74	4785.0
75	4102.5
76	3420.0
77	2564.0
78	1708.0
79	1178.5
80	649.0
81	649.0
82	505.5
83	362.0
84	282.5
85	203.0
86	150.0
87	97.0
88	65.0
89	33.0
90	22.0
91	11.0
92	8.0
93	5.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	734165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.70730373936342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63721122058573	14.523828917806112
2	9.539871951632023	3.5693056446762785
3	3.3291281011678566	1.8683703172739188
4	1.5615706974240644	1.1685110938880618
5	0.9558179271332482	0.8940388141205204
6	0.6001539967887487	0.6736357864992037
7	0.4221401294119713	0.552797253503875
8	0.3329383937489367	0.4982703726685707
9	0.24529867608152964	0.41299891562888064
>10	2.7660491127880973	12.247745498772383
>50	1.2446832860057166	17.225464990218274
>100	1.3534561948707324	39.5921301122172
>500	0.0051101366580879855	0.6810594766293925
>1k	0.0051101366580879855	2.5524366589539493
>5k	7.30019522583998E-4	0.691165432368429
>10k+	7.30019522583998E-4	2.848240714774937
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20856	2.840778299156184	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5061	0.6893545728821178	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3904	0.5317605715336471	No Hit
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTT	3393	0.4621576893477624	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTAT	3150	0.4290588627896999	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGT	2539	0.3458350643247771	No Hit
AATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGT	2512	0.34215741692943685	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2083	0.2837236860923634	No Hit
AAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGT	1109	0.15105596153453243	No Hit
ATGTTAGGGGGGGAGGGGAACTGGTATCTCCCAAGATTAACCTTCA	940	0.12803661302295807	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT	797	0.10855870274393357	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	736	0.10024994381372034	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5116016154406707	0.0
2	0.0	0.0	0.0	0.6687869893007703	0.0
3	0.0	0.0	0.0	0.9162790380908924	0.0
4	0.0	0.0	0.0	2.0662929995300785	0.0
5	0.0	0.0	0.0	2.2312422956692295	0.0
6	0.0	0.0	0.0	2.9309487649234165	0.0
7	0.0	0.0	0.0	3.423617306736224	0.0
8	0.0	0.0	0.0	3.670428309712394	0.0
9	0.0	0.0	0.0	4.549658455524303	0.0
10	0.0	0.0	0.0	5.352883888499179	0.0
11	0.0	0.0	0.0	6.512568700496482	0.0
12	0.0	0.0	0.0	6.945305210681522	0.0
13	0.0	0.0	0.0	7.136270456913636	0.0
14	0.0	0.0	0.0	7.368234661145655	0.0
15	0.0	0.0	0.0	7.522968270075528	0.0
16	0.0	0.0	0.0	7.804921237051617	0.0
17	0.0	0.0	0.0	8.139723359190373	0.0
18	0.0	0.0	0.0	8.658816478584514	0.0
19	0.0	0.0	0.0	8.85754564709568	0.0
20	0.0	0.0	0.0	9.055048933141732	0.0
21	0.0	0.0	0.0	9.29028215728072	0.0
22	0.0	0.0	0.0	9.527694728024354	0.0
23	0.0	0.0	0.0	9.752984683279644	0.0
24	0.0	0.0	0.0	9.911804567093228	0.0
25	1.362091627903809E-4	0.0	0.0	10.054824188023128	0.0
26	1.362091627903809E-4	0.0	0.0	10.192667860766994	0.0
27	1.362091627903809E-4	0.0	0.0	10.36660696165031	0.0
28	1.362091627903809E-4	0.0	0.0	10.51357664830113	0.0
29	1.362091627903809E-4	0.0	0.0	10.67416725123099	0.0
30	1.362091627903809E-4	0.0	0.0	10.89945720648628	0.0
31	1.362091627903809E-4	0.0	0.0	11.055008070392896	0.0
32	1.362091627903809E-4	0.0	0.0	11.21369174504369	0.0
33	1.362091627903809E-4	0.0	0.0	11.376189276252614	0.0
34	1.362091627903809E-4	0.0	0.0	11.532829813461552	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	30	4.8651073E-6	40.000004	1
TTTGTCG	30	4.8651073E-6	40.000004	14
GACGTAA	30	4.8651073E-6	40.000004	22
CGCATGA	30	4.8651073E-6	40.000004	13
ATAGCCG	30	4.8651073E-6	40.000004	1
CACGCGA	30	4.8651073E-6	40.000004	30
GTGATCC	30	4.8651073E-6	40.000004	22
CGATTAG	30	4.8651073E-6	40.000004	1
CGCGACG	30	4.8651073E-6	40.000004	32
ACCTCGA	30	4.8651073E-6	40.000004	17
GGCTTAA	30	4.8651073E-6	40.000004	25
TGCGCAT	30	4.8651073E-6	40.000004	11
TACCTCG	30	4.8651073E-6	40.000004	1
CGTAAAT	30	4.8651073E-6	40.000004	24
TCGAGGT	30	4.8651073E-6	40.000004	19
ATTGCAA	30	4.8651073E-6	40.000004	32
TTACCGG	60	0.0	40.000004	2
AGTACGA	30	4.8651073E-6	40.000004	33
TCAACGA	30	4.8651073E-6	40.000004	19
GTACGAC	30	4.8651073E-6	40.000004	34
>>END_MODULE