FastQCFastQC Report
Sat 18 Jun 2016
SRR3548383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688049
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189772.7580884500958507No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52250.7593935897007336No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37940.5514142161386762No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTAT22980.33398784098225565No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGT22430.3259942242485637No Hit
AATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGT20850.3030307434499578No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20610.29954261978434676No Hit
CTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT16510.23995384049682508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAC304.8646616E-640.00000420
CGAACGT304.8646616E-640.00000432
ACGTTCG304.8646616E-640.00000417
GCGAAGT304.8646616E-640.00000438
TAGGTTA304.8646616E-640.00000413
TTTCGCG304.8646616E-640.0000041
CCGTGTA304.8646616E-640.00000411
CGTCTCA304.8646616E-640.00000429
TACGCGA304.8646616E-640.00000410
CTCGGTT304.8646616E-640.0000049
CACTACG304.8646616E-640.0000041
CGCTACG600.040.0000041
ATCCCGC304.8646616E-640.00000428
TCGCTAG304.8646616E-640.00000415
GTGTAAC304.8646616E-640.00000412
GCGATGC304.8646616E-640.0000049
TTAACGA304.8646616E-640.00000418
CGTAAGC304.8646616E-640.00000412
CGTAACG304.8646616E-640.0000041
CAGTCGC304.8646616E-640.00000425