FastQCFastQC Report
Sat 18 Jun 2016
SRR3548382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912480
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160731.7614632649482729No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38800.42521479922847627No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29600.3243906715763633No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19780.2167718744520428No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC14950.1638392074346835No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT14380.1575924951779765No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT14080.15430475188497284RNA PCR Primer, Index 38 (96% over 26bp)
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT12550.13753726109065403RNA PCR Primer, Index 27 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA207.03252E-445.00000440
ACCGGTA207.03252E-445.0000044
GTCGACA207.03252E-445.00000420
CGAAACG406.8121153E-945.0000041
CACGCGT207.03252E-445.00000428
CCTACGT207.03252E-445.00000412
TGCGGTC406.8121153E-945.00000420
TGACGTA207.03252E-445.00000439
CGACAAG750.045.0000041
TAGTTCG207.03252E-445.00000418
ATCCGAA207.03252E-445.00000412
TCAATCG207.03252E-445.0000042
TCGGACG351.2115561E-745.0000041
ACTACCG207.03252E-445.0000041
GATTGCG207.03252E-445.0000041
GTAAGTA351.2115561E-745.00000443
TCGACAT207.03252E-445.00000421
CGTTTCA351.2115561E-745.00000422
CGATAGA207.03252E-445.00000411
CTAATCG207.03252E-445.00000427