Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548382_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 912480 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 16073 | 1.7614632649482729 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3880 | 0.42521479922847627 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2960 | 0.3243906715763633 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1978 | 0.2167718744520428 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC | 1495 | 0.1638392074346835 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT | 1438 | 0.1575924951779765 | No Hit |
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT | 1408 | 0.15430475188497284 | RNA PCR Primer, Index 38 (96% over 26bp) |
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT | 1255 | 0.13753726109065403 | RNA PCR Primer, Index 27 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGACAA | 20 | 7.03252E-4 | 45.000004 | 40 |
ACCGGTA | 20 | 7.03252E-4 | 45.000004 | 4 |
GTCGACA | 20 | 7.03252E-4 | 45.000004 | 20 |
CGAAACG | 40 | 6.8121153E-9 | 45.000004 | 1 |
CACGCGT | 20 | 7.03252E-4 | 45.000004 | 28 |
CCTACGT | 20 | 7.03252E-4 | 45.000004 | 12 |
TGCGGTC | 40 | 6.8121153E-9 | 45.000004 | 20 |
TGACGTA | 20 | 7.03252E-4 | 45.000004 | 39 |
CGACAAG | 75 | 0.0 | 45.000004 | 1 |
TAGTTCG | 20 | 7.03252E-4 | 45.000004 | 18 |
ATCCGAA | 20 | 7.03252E-4 | 45.000004 | 12 |
TCAATCG | 20 | 7.03252E-4 | 45.000004 | 2 |
TCGGACG | 35 | 1.2115561E-7 | 45.000004 | 1 |
ACTACCG | 20 | 7.03252E-4 | 45.000004 | 1 |
GATTGCG | 20 | 7.03252E-4 | 45.000004 | 1 |
GTAAGTA | 35 | 1.2115561E-7 | 45.000004 | 43 |
TCGACAT | 20 | 7.03252E-4 | 45.000004 | 21 |
CGTTTCA | 35 | 1.2115561E-7 | 45.000004 | 22 |
CGATAGA | 20 | 7.03252E-4 | 45.000004 | 11 |
CTAATCG | 20 | 7.03252E-4 | 45.000004 | 27 |