##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912480
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211195861827107	31.0	31.0	34.0	30.0	34.0
2	31.393956031913028	31.0	31.0	34.0	30.0	34.0
3	31.58958004559004	31.0	31.0	34.0	30.0	34.0
4	35.52631948097493	37.0	35.0	37.0	33.0	37.0
5	35.117780115728564	37.0	35.0	37.0	32.0	37.0
6	35.115198141329124	37.0	35.0	37.0	32.0	37.0
7	34.34397795020165	37.0	35.0	37.0	32.0	37.0
8	34.6915406365071	37.0	35.0	37.0	32.0	37.0
9	35.83408403471857	39.0	35.0	39.0	31.0	39.0
10	35.49552318954936	37.0	35.0	39.0	30.0	39.0
11	35.93710108714711	37.0	35.0	39.0	31.0	39.0
12	35.83270427844994	37.0	35.0	39.0	31.0	39.0
13	35.63102314571278	37.0	35.0	39.0	30.0	39.0
14	36.74540263896195	39.0	35.0	40.0	31.0	41.0
15	36.8161800806593	38.0	35.0	40.0	31.0	41.0
16	36.796558828686656	38.0	35.0	40.0	31.0	41.0
17	36.759649526564964	38.0	35.0	40.0	31.0	41.0
18	36.57165855689988	38.0	35.0	40.0	31.0	41.0
19	36.53551858670875	38.0	35.0	40.0	31.0	41.0
20	36.480786428195685	38.0	35.0	40.0	31.0	41.0
21	36.499526564965805	38.0	35.0	40.0	31.0	41.0
22	36.47921159915834	38.0	35.0	40.0	31.0	41.0
23	36.38461007364545	38.0	35.0	40.0	31.0	41.0
24	35.994370287567946	38.0	34.0	40.0	30.0	41.0
25	36.03458157987024	38.0	34.0	40.0	30.0	41.0
26	35.870831141504475	38.0	34.0	40.0	30.0	41.0
27	35.71244410836402	38.0	34.0	40.0	29.0	41.0
28	35.68082149745748	38.0	34.0	40.0	29.0	41.0
29	35.40167894090829	38.0	34.0	40.0	27.0	41.0
30	35.481616035419954	38.0	34.0	40.0	28.0	41.0
31	35.34464536209013	38.0	34.0	40.0	27.0	41.0
32	35.25875416447484	38.0	34.0	40.0	27.0	41.0
33	35.19845147290899	38.0	34.0	40.0	27.0	41.0
34	35.13079190776784	38.0	34.0	40.0	27.0	41.0
35	35.01012296159916	38.0	34.0	40.0	26.0	41.0
36	34.88079519551113	37.0	33.0	40.0	25.0	41.0
37	34.78226920042083	37.0	33.0	40.0	25.0	41.0
38	34.64798899701911	37.0	33.0	40.0	25.0	41.0
39	34.576440031562335	37.0	33.0	40.0	24.0	41.0
40	34.366995440995964	37.0	33.0	40.0	23.0	41.0
41	34.35989062773979	37.0	33.0	40.0	24.0	41.0
42	34.26849793968087	37.0	33.0	40.0	23.0	41.0
43	34.21611432579344	37.0	33.0	40.0	23.0	41.0
44	34.133618271085396	36.0	33.0	40.0	23.0	41.0
45	33.97248158863756	36.0	33.0	40.0	23.0	41.0
46	33.86986454497633	36.0	33.0	40.0	23.0	41.0
47	33.7161800806593	36.0	33.0	39.0	23.0	41.0
48	33.5736673680519	35.0	33.0	39.0	23.0	40.0
49	33.416143915483076	35.0	32.0	39.0	22.0	40.0
50	33.23166754339821	35.0	32.0	39.0	21.0	40.0
51	31.3333322374189	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	0.0
10	17.0
11	29.0
12	30.0
13	34.0
14	87.0
15	200.0
16	331.0
17	620.0
18	1129.0
19	1918.0
20	3025.0
21	4361.0
22	5911.0
23	7530.0
24	8933.0
25	10612.0
26	12064.0
27	14429.0
28	17165.0
29	21401.0
30	26375.0
31	33482.0
32	43526.0
33	58760.0
34	88005.0
35	91787.0
36	94725.0
37	125822.0
38	154570.0
39	85597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.440689110994214	33.87855076275644	18.932469752761705	25.748290373487638
2	23.434267052428545	32.64641416798176	20.004602840610204	23.914715938979484
3	20.479024197790636	33.99274504646677	19.325683850604943	26.20254690513765
4	20.052603892688058	34.642074346835	18.423417499561634	26.881904260915306
5	18.388677012098896	36.99620813606874	16.6973522707347	27.917762581097666
6	18.99877257583728	39.86542170787305	18.994498509556372	22.1413072067333
7	82.31687269857969	11.236081886726284	3.533337716991057	2.913707697702963
8	84.97764334560758	9.045239347711732	3.124013677012099	2.8531036296685954
9	79.58804576538664	12.930256005611081	4.571497457478521	2.9102007715237592
10	40.28428020340172	36.30227511835876	10.309267052428545	13.104177625810976
11	32.17418463966333	29.860161318604245	21.75883307031387	16.20682097141855
12	30.630808346484308	26.98974224092583	25.140715412940555	17.23873399964931
13	23.81060406803437	34.024526564965804	23.595366473785724	18.5695028932141
14	19.512208486761352	35.40220059617745	25.11550938102753	19.970081536033664
15	18.672518849728213	31.1625460284061	31.075859196913907	19.08907592495178
16	20.811415044713307	28.55777660880238	30.73042696826232	19.900381378221986
17	21.032132211116956	27.949872873926	25.49907943187796	25.51891548307908
18	22.683675258635805	30.21436086270384	25.49020252498685	21.611761353673504
19	23.578489391548306	31.916425565491846	23.65257320708399	20.852511835875855
20	26.46984043485885	30.173154480098198	22.996668420129758	20.360336664913202
21	24.305628616517623	29.76700859196914	24.673198316675435	21.254164474837804
22	22.80630808346484	28.392183938278098	24.147597755567247	24.653910222689813
23	23.257715237594248	31.244410836401894	22.801705242854638	22.69616868314922
24	22.368599859722956	30.614917587234793	26.47970366473786	20.5367788883044
25	21.26797299666842	29.826516745572505	25.423790110468175	23.4817201472909
26	23.25135893389444	33.77838418376293	21.73888742766965	21.23136945467298
27	20.35189812379449	32.54405576012625	25.299951779765035	21.80409433631422
28	21.190491846396633	32.04409959670349	26.18709451166053	20.57831404523935
29	20.87256706996318	31.19619060143784	26.560691741188847	21.370550587410133
30	24.14189900052604	29.657526740312118	25.70642644222339	20.494147816938455
31	25.721878835700508	30.534039102226902	21.91817902858145	21.825903033491144
32	24.53708574434508	31.675762756443977	24.176639487988776	19.610512011222163
33	24.352314571278274	31.32309749254778	23.05343678765562	21.271151148518324
34	22.30372172540768	31.48978607750307	25.014466070489217	21.192026126600037
35	23.573229002279504	30.734481851657026	25.461927932667017	20.23036121339646
36	25.318363142205857	30.859744871120466	24.539935121865685	19.281956864807995
37	23.85608451692092	30.867306680694373	25.063672628441168	20.21293617394354
38	23.72402682798527	30.51803875153428	23.940469928108012	21.817464492372434
39	23.23108451692092	28.889948272838854	25.60472558302648	22.274241627213748
40	24.137734525688234	30.0015342802034	25.084604594073294	20.77612660003507
41	20.611520252498686	27.726635104331056	27.694305628616515	23.967539014553743
42	26.03454322286516	28.216508854988604	23.845015781167806	21.903932140978434
43	23.14439768542872	29.158228125547957	24.7346791162546	22.962695072768717
44	22.421642118183414	30.048987375065757	24.756049447659127	22.773321059091707
45	23.067135718043136	30.814593196563212	25.32877432930037	20.789496756093286
46	23.229111870945115	29.35976678940908	26.667981763983867	20.74313957566193
47	23.442267227774856	30.040439242503947	25.02783622654743	21.48945730317377
48	24.616101174820272	27.538904962300542	26.43860687357531	21.406386989303876
49	22.209582675784674	27.854089952656498	26.816587760827638	23.119739610731195
50	20.817552165526916	30.424009293354377	25.796510608451694	22.961927932667017
51	21.843108890057863	29.523277222514466	26.322220761002978	22.31139312642469
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18788.0
1	14540.5
2	10293.0
3	6770.0
4	3247.0
5	2807.5
6	2368.0
7	2436.5
8	2505.0
9	2686.0
10	2867.0
11	2815.5
12	2764.0
13	2864.0
14	2964.0
15	3167.5
16	3371.0
17	3390.0
18	3409.0
19	3355.5
20	3302.0
21	3492.5
22	3683.0
23	4254.0
24	4825.0
25	5012.5
26	7360.5
27	9521.0
28	11686.5
29	13852.0
30	14281.5
31	14711.0
32	17458.0
33	20205.0
34	21793.5
35	23382.0
36	26085.0
37	28788.0
38	31399.0
39	34010.0
40	37614.0
41	41218.0
42	47737.5
43	54257.0
44	58404.0
45	62551.0
46	63945.0
47	65339.0
48	63304.0
49	61269.0
50	59985.0
51	58701.0
52	56269.5
53	53838.0
54	50014.5
55	46191.0
56	45104.0
57	44017.0
58	41602.5
59	39188.0
60	40015.5
61	40843.0
62	37201.0
63	33559.0
64	31865.5
65	30172.0
66	25316.0
67	20460.0
68	17223.0
69	13986.0
70	12313.5
71	10641.0
72	9246.5
73	7852.0
74	6468.0
75	4668.0
76	4252.0
77	3293.5
78	2335.0
79	1698.5
80	1062.0
81	881.5
82	701.0
83	561.5
84	422.0
85	326.5
86	231.0
87	177.0
88	123.0
89	98.5
90	74.0
91	55.0
92	36.0
93	25.0
94	14.0
95	8.5
96	3.0
97	4.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	912480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.199643421758385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70325148925674	17.693837626162328
2	8.830282077698156	3.920582268768856
3	3.0182184783842776	2.010101219672794
4	1.4465287673551743	1.284496913384022
5	0.8505253046407684	0.9440679242103739
6	0.5396332494138759	0.718779942930771
7	0.36386220774887507	0.5654327886675158
8	0.27717027222618346	0.4922464968426379
9	0.21512535172752614	0.4298135489398291
>10	2.5146226914234697	13.453452288401053
>50	1.186980469634546	19.21116477889459
>100	1.0492421216223127	35.772548614249025
>500	5.06390985338954E-4	0.0762187212981897
>1k	0.0035447368973726776	1.6203785380414548
>5k	0.0	0.0
>10k+	5.06390985338954E-4	1.8068783295365827
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16073	1.7614632649482729	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3880	0.42521479922847627	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2960	0.3243906715763633	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1978	0.2167718744520428	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	1495	0.1638392074346835	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	1438	0.1575924951779765	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1408	0.15430475188497284	RNA PCR Primer, Index 38 (96% over 26bp)
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	1255	0.13753726109065403	RNA PCR Primer, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0959144310012274E-4	0.0	0.0	0.22082675784674732	0.0
2	1.0959144310012274E-4	0.0	0.0	0.28592407504822026	0.0
3	1.0959144310012274E-4	0.0	0.0	0.3760082412765211	0.0
4	1.0959144310012274E-4	0.0	0.0	0.9298833947045415	0.0
5	1.0959144310012274E-4	0.0	0.0	1.0094467823952307	0.0
6	1.0959144310012274E-4	0.0	0.0	1.3993731369454674	0.0
7	1.0959144310012274E-4	0.0	0.0	1.6823382430299842	0.0
8	1.0959144310012274E-4	0.0	0.0	1.8684245134139927	0.0
9	1.0959144310012274E-4	0.0	0.0	2.3281606172190075	0.0
10	1.0959144310012274E-4	0.0	0.0	2.788335086796423	0.0
11	1.0959144310012274E-4	0.0	0.0	3.4064308258811153	0.0
12	1.0959144310012274E-4	0.0	0.0	3.626600035069262	0.0
13	1.0959144310012274E-4	0.0	0.0	3.7252323338593722	0.0
14	1.0959144310012274E-4	0.0	0.0	3.840084166228301	0.0
15	1.0959144310012274E-4	0.0	0.0	3.926880589163598	0.0
16	1.0959144310012274E-4	0.0	0.0	4.093349991232684	0.0
17	2.191828862002455E-4	0.0	0.0	4.294121514992109	0.0
18	2.191828862002455E-4	0.0	0.0	4.53555146414168	0.0
19	2.191828862002455E-4	0.0	0.0	4.645800455900403	0.0
20	2.191828862002455E-4	0.0	0.0	4.767118183412239	0.0
21	2.191828862002455E-4	0.0	0.0	4.904874627389093	0.0
22	2.191828862002455E-4	0.0	0.0	5.048439417850254	0.0
23	2.191828862002455E-4	0.0	0.0	5.204388041381729	0.0
24	2.191828862002455E-4	0.0	0.0	5.317267227774855	0.0
25	2.191828862002455E-4	0.0	0.0	5.421050324390672	0.0
26	2.191828862002455E-4	0.0	0.0	5.513435910924075	0.0
27	3.287743293003682E-4	0.0	0.0	5.61568472733649	0.0
28	3.287743293003682E-4	0.0	0.0	5.711248465719796	0.0
29	3.287743293003682E-4	0.0	0.0	5.8141548307908115	0.0
30	3.287743293003682E-4	0.0	0.0	5.95508942661757	0.0
31	3.287743293003682E-4	0.0	0.0	6.074653691039804	0.0
32	3.287743293003682E-4	0.0	0.0	6.1923549009293355	0.0
33	3.287743293003682E-4	0.0	0.0	6.298001052077853	0.0
34	3.287743293003682E-4	0.0	0.0	6.41033228125548	0.0
35	3.287743293003682E-4	0.0	0.0	6.541294055760126	0.0
36	3.287743293003682E-4	0.0	0.0	6.657132211116956	0.0
37	4.38365772400491E-4	0.0	0.0	6.781299316149395	0.0
38	4.38365772400491E-4	0.0	0.0	6.9063431527266355	0.0
39	5.479572155006137E-4	0.0	0.0	7.034565141153779	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	20	7.03252E-4	45.000004	40
ACCGGTA	20	7.03252E-4	45.000004	4
GTCGACA	20	7.03252E-4	45.000004	20
CGAAACG	40	6.8121153E-9	45.000004	1
CACGCGT	20	7.03252E-4	45.000004	28
CCTACGT	20	7.03252E-4	45.000004	12
TGCGGTC	40	6.8121153E-9	45.000004	20
TGACGTA	20	7.03252E-4	45.000004	39
CGACAAG	75	0.0	45.000004	1
TAGTTCG	20	7.03252E-4	45.000004	18
ATCCGAA	20	7.03252E-4	45.000004	12
TCAATCG	20	7.03252E-4	45.000004	2
TCGGACG	35	1.2115561E-7	45.000004	1
ACTACCG	20	7.03252E-4	45.000004	1
GATTGCG	20	7.03252E-4	45.000004	1
GTAAGTA	35	1.2115561E-7	45.000004	43
TCGACAT	20	7.03252E-4	45.000004	21
CGTTTCA	35	1.2115561E-7	45.000004	22
CGATAGA	20	7.03252E-4	45.000004	11
CTAATCG	20	7.03252E-4	45.000004	27
>>END_MODULE