FastQCFastQC Report
Sat 18 Jun 2016
SRR3548381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979260
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198792.0300022465943672No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49110.5015011335089761No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36740.37518125931826074No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT24560.2508016257173784RNA PCR Primer, Index 38 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22630.23109286604170495No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTAT12550.1281579968547679No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGT11410.11651655331576904No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT10540.10763229377284889No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAT200.001255677540.00000439
TCGTTAC200.001255677540.00000432
ACGTTCC200.001255677540.00000414
CTATCCG200.001255677540.0000041
CGGCATG401.927765E-840.0000041
GACCGTC200.001255677540.00000425
GACGTAA200.001255677540.00000428
TAGCGGT401.927765E-840.00000422
ATAGCGG401.927765E-840.0000042
TCCTCGA200.001255677540.00000424
ATAGACG200.001255677540.0000041
TATGCGA200.001255677540.00000439
CGTTACA200.001255677540.00000433
ACTAGTA401.927765E-840.00000427
CTAACGC200.001255677540.00000428
TAACCGA200.001255677540.00000426
CGTAGTT200.001255677540.00000426
CGTAGAC200.001255677540.00000413
CTACGAC200.001255677540.00000437
CGCTAGA401.927765E-840.00000412