##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	979260
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10227620856565	31.0	31.0	34.0	28.0	34.0
2	31.41388191083063	31.0	31.0	34.0	28.0	34.0
3	31.56016890304924	31.0	31.0	34.0	30.0	34.0
4	35.52636480607806	37.0	35.0	37.0	33.0	37.0
5	35.10000816943406	37.0	35.0	37.0	32.0	37.0
6	35.25784674141699	37.0	35.0	37.0	32.0	37.0
7	35.25308294017932	37.0	35.0	37.0	32.0	37.0
8	35.67865531115332	37.0	35.0	37.0	35.0	37.0
9	37.025778649184076	39.0	37.0	39.0	33.0	39.0
10	36.2120989318465	38.0	35.0	39.0	32.0	39.0
11	36.447943344974775	38.0	35.0	39.0	32.0	39.0
12	36.22133243469558	38.0	35.0	39.0	32.0	39.0
13	35.87640463206911	38.0	35.0	39.0	30.0	39.0
14	37.10764148438617	39.0	36.0	40.0	32.0	41.0
15	37.03991993954619	39.0	35.0	40.0	32.0	41.0
16	36.95973694422319	39.0	35.0	40.0	32.0	41.0
17	36.95985744337561	38.0	35.0	40.0	32.0	41.0
18	36.77759839062149	38.0	35.0	40.0	31.0	41.0
19	36.989587035108144	39.0	35.0	40.0	32.0	41.0
20	36.778861589363395	38.0	35.0	40.0	31.0	41.0
21	36.878366317423364	39.0	35.0	40.0	31.0	41.0
22	36.97247411310582	39.0	35.0	40.0	32.0	41.0
23	36.79491554847538	39.0	35.0	40.0	31.0	41.0
24	36.371920633948086	38.0	35.0	40.0	30.0	41.0
25	36.538748646937485	38.0	35.0	40.0	31.0	41.0
26	36.53025141433327	38.0	35.0	40.0	31.0	41.0
27	36.40036762453281	38.0	35.0	40.0	31.0	41.0
28	36.450743418499684	38.0	35.0	40.0	31.0	41.0
29	36.116950554500335	38.0	35.0	40.0	30.0	41.0
30	36.20153279006597	38.0	35.0	40.0	30.0	41.0
31	36.15853501623675	38.0	35.0	40.0	30.0	41.0
32	36.12551007903927	38.0	35.0	40.0	30.0	41.0
33	36.09676388293201	38.0	35.0	40.0	30.0	41.0
34	36.0454935359353	38.0	35.0	40.0	30.0	41.0
35	35.83465984518922	38.0	35.0	40.0	30.0	41.0
36	35.8190715438188	38.0	35.0	40.0	30.0	41.0
37	35.72128954516676	38.0	35.0	40.0	29.0	41.0
38	35.53705042583175	38.0	34.0	40.0	29.0	41.0
39	35.526555766599266	38.0	34.0	40.0	29.0	41.0
40	35.31806976696689	38.0	34.0	40.0	27.0	41.0
41	35.364784633294526	38.0	34.0	40.0	28.0	41.0
42	35.24709474501154	37.0	34.0	40.0	28.0	41.0
43	35.19173253272879	37.0	34.0	40.0	27.0	41.0
44	35.12185323611707	37.0	34.0	40.0	27.0	41.0
45	34.940156853134	37.0	34.0	40.0	27.0	41.0
46	33.92668035046872	36.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	4.0
10	9.0
11	13.0
12	17.0
13	26.0
14	49.0
15	95.0
16	131.0
17	268.0
18	467.0
19	828.0
20	1454.0
21	2378.0
22	3460.0
23	4735.0
24	6240.0
25	7876.0
26	9402.0
27	11178.0
28	13718.0
29	17359.0
30	21975.0
31	28579.0
32	38506.0
33	54830.0
34	89063.0
35	100864.0
36	95772.0
37	132753.0
38	197366.0
39	139839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.004227682127322	33.224271388599554	19.135367522414885	26.636133406858242
2	23.990257949880522	31.89306210812246	19.389743275534588	24.726936666462432
3	20.3871290566346	33.92439188775197	19.207769131793395	26.48070992382003
4	19.605926924412312	34.61481118395523	18.496211424953536	27.283050466678922
5	18.671445785593203	36.64981720891286	16.793292894634725	27.88544411085922
6	19.253211608765803	39.237587566121356	19.077977248126135	22.431223576986703
7	83.89099932602169	9.227375773543288	3.8223760798970647	3.0592488205379573
8	85.50742397320425	8.178828911627146	3.373261442313584	2.940485672855013
9	80.72197373526949	11.176092151216224	4.990298797050834	3.111635316463452
10	39.896350305332604	36.05610358842391	10.56542695504769	13.482119151195802
11	31.912158160243447	29.54373710761187	22.054306312930173	16.48979841921451
12	30.546943610481385	26.720482813553094	25.35424708453322	17.378326491432304
13	23.73588219676082	33.76886628678798	23.927251189673836	18.568000326777362
14	19.52750035741274	34.880317790985025	25.418172906071934	20.174008945530296
15	18.683495700835326	30.822355656311913	31.183342523946656	19.31080611890611
16	20.684598574433757	28.53930518963299	30.747809570492002	20.028286665441254
17	20.870248963503055	27.838163511222756	25.751690051671673	25.53989747360252
18	22.673549414864286	29.96762861752752	25.52897085554398	21.829851112064212
19	23.33425239466536	31.935543165247225	23.96033739762678	20.769867042460632
20	26.1339174478688	29.953638461695565	23.487225047484834	20.425219042950797
21	23.98566264322039	29.894716418520105	24.9529236362151	21.1666973020444
22	22.79558033617221	28.30382125278271	24.291812184710903	24.60878622633417
23	22.901170271429447	31.38369789432837	22.994199701815656	22.720932132426526
24	22.13375405918755	30.511406572309703	26.54616751424545	20.808671854257295
25	21.040990135408368	29.878071196617856	25.72340338623042	23.35753528174336
26	23.01799317852256	33.59189592141004	22.095766190797132	21.294344709270266
27	20.30890672548659	32.456344586728754	25.340052692849703	21.894695994934953
28	21.05845230071687	31.79421195596675	26.553111533198535	20.594224210117844
29	20.941323039846417	31.15362620754447	26.600800604538122	21.30425014807099
30	23.936543920919878	29.685272552743907	25.80673161366746	20.57145191266875
31	25.497416416477748	30.56593754467659	22.181749484304476	21.754896554541183
32	24.630230990748117	31.458039744296713	24.225231297101892	19.686497967853278
33	23.983313930927437	31.369401384719076	23.209770643138697	21.437514041214794
34	22.364846925229255	31.174968854032635	25.267344729693853	21.192839491044257
35	23.426873353348444	30.78089577844495	25.624348998223144	20.167881869983457
36	25.17860425219043	30.747605334640443	24.845699814145377	19.228090599023755
37	23.68849947919858	30.6965463717501	25.23609664440496	20.378857504646366
38	23.466597226477134	30.39264342462676	24.34450503441374	21.796254314482365
39	23.144925760267956	28.739354206237362	25.794783816351124	22.320936217143558
40	23.851275452893	29.93678900394175	25.271837918428204	20.940097624737046
41	20.661009333578416	27.571533607009375	27.769233911320796	23.998223148091416
42	26.090108857708884	28.138492330943777	23.88528072217797	21.886118089169372
43	22.889528827890448	29.213691971488675	25.017053693605373	22.879725507015504
44	22.375262953658886	29.94301819741437	24.758184751751322	22.92353409717542
45	22.946919102179198	30.606274125359967	25.705532749218797	20.741274023242042
46	23.014112697342892	29.183873537160714	26.95617098625493	20.84584277924147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23160.0
1	17991.5
2	12823.0
3	8338.0
4	3853.0
5	3508.5
6	3164.0
7	3164.0
8	3149.0
9	3134.0
10	3155.0
11	3176.0
12	3130.0
13	3084.0
14	3018.5
15	2953.0
16	3010.5
17	3068.0
18	3320.0
19	3572.0
20	3572.0
21	4060.0
22	4548.0
23	4878.0
24	5208.0
25	5892.5
26	6577.0
27	8937.5
28	11298.0
29	12149.0
30	13000.0
31	13000.0
32	17594.0
33	22188.0
34	23173.5
35	24159.0
36	27560.0
37	30961.0
38	34562.5
39	38164.0
40	43170.0
41	48176.0
42	53863.5
43	59551.0
44	59551.0
45	67814.0
46	76077.0
47	78483.5
48	80890.0
49	79812.0
50	78734.0
51	71573.0
52	64412.0
53	61150.5
54	57889.0
55	54297.0
56	50705.0
57	50705.0
58	50029.5
59	49354.0
60	49675.0
61	49996.0
62	45797.0
63	41598.0
64	38210.5
65	34823.0
66	29008.0
67	23193.0
68	19159.5
69	15126.0
70	15126.0
71	13432.0
72	11738.0
73	10045.5
74	8353.0
75	6723.0
76	5093.0
77	4096.5
78	3100.0
79	2277.5
80	1455.0
81	1455.0
82	995.0
83	535.0
84	383.5
85	232.0
86	158.5
87	85.0
88	52.5
89	20.0
90	17.5
91	15.0
92	15.0
93	15.0
94	15.0
95	9.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	979260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.40283848871229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.87268630305756	13.378029439255565
2	9.588909406998475	3.3374848338577676
3	3.283336092362383	1.7141810315862684
4	1.6140518799009171	1.1235633671327243
5	1.004729938431016	0.8742576421644407
6	0.6206666493189352	0.6480816872056596
7	0.45176114573635356	0.5503348378307763
8	0.3481016041198435	0.4846364795327441
9	0.2403283894350595	0.37641565310316205
>10	3.015649844213258	12.568742217225534
>50	1.4098686542169894	17.93894050358799
>100	1.5428193864880801	42.95632209634518
>500	0.0023635685737082805	0.2819653587056289
>1k	0.00413624500398949	1.7228474178534305
>5k	0.0	0.0
>10k+	5.908921434270701E-4	2.044197434613128
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19879	2.0300022465943672	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4911	0.5015011335089761	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3674	0.37518125931826074	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	2456	0.2508016257173784	RNA PCR Primer, Index 38 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2263	0.23109286604170495	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTAT	1255	0.1281579968547679	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGT	1141	0.11651655331576904	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	1054	0.10763229377284889	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0423585156138308E-4	0.0	0.0	0.28307089026407695	0.0
2	2.0423585156138308E-4	0.0	0.0	0.3789596225721463	0.0
3	2.0423585156138308E-4	0.0	0.0	0.515797643118273	0.0
4	2.0423585156138308E-4	0.0	0.0	1.1313644997242815	0.0
5	2.0423585156138308E-4	0.0	0.0	1.2317464207667015	0.0
6	2.0423585156138308E-4	0.0	0.0	1.706594775646917	0.0
7	2.0423585156138308E-4	0.0	0.0	2.051855482711435	0.0
8	2.0423585156138308E-4	0.0	0.0	2.2799869289055	0.0
9	2.0423585156138308E-4	0.0	0.0	2.7479933827584095	0.0
10	2.0423585156138308E-4	0.0	0.0	3.3322100361497458	0.0
11	2.0423585156138308E-4	0.0	0.0	4.013336601106959	0.0
12	2.0423585156138308E-4	0.0	0.0	4.298858321589772	0.0
13	2.0423585156138308E-4	0.0	0.0	4.419561709862549	0.0
14	2.0423585156138308E-4	0.0	0.0	4.538120621693932	0.0
15	2.0423585156138308E-4	0.0	0.0	4.6400343116230625	0.0
16	2.0423585156138308E-4	0.0	0.0	4.841615097114148	0.0
17	2.0423585156138308E-4	0.0	0.0	5.075056675448808	0.0
18	2.0423585156138308E-4	0.0	0.0	5.385903641525234	0.0
19	2.0423585156138308E-4	0.0	0.0	5.518861180891694	0.0
20	2.0423585156138308E-4	0.0	0.0	5.661928394910443	0.0
21	2.0423585156138308E-4	0.0	0.0	5.838183934807916	0.0
22	2.0423585156138308E-4	0.0	0.0	6.020464432326451	0.0
23	2.0423585156138308E-4	0.0	0.0	6.208361415762923	0.0
24	2.0423585156138308E-4	0.0	0.0	6.342748606090313	0.0
25	2.0423585156138308E-4	0.0	0.0	6.461409635847477	0.0
26	2.0423585156138308E-4	0.0	0.0	6.573535118354676	0.0
27	2.0423585156138308E-4	0.0	0.0	6.691787676408717	0.0
28	2.0423585156138308E-4	0.0	0.0	6.807895758021363	0.0
29	2.0423585156138308E-4	0.0	0.0	6.943099891754999	0.0
30	2.0423585156138308E-4	0.0	0.0	7.1246655637930685	0.0
31	3.0635377734207465E-4	0.0	0.0	7.263852296632151	0.0
32	3.0635377734207465E-4	0.0	0.0	7.398443722811102	0.0
33	3.0635377734207465E-4	0.0	0.0	7.524559361150256	0.0
34	3.0635377734207465E-4	0.0	0.0	7.652411004227682	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	20	0.0012556775	40.000004	39
TCGTTAC	20	0.0012556775	40.000004	32
ACGTTCC	20	0.0012556775	40.000004	14
CTATCCG	20	0.0012556775	40.000004	1
CGGCATG	40	1.927765E-8	40.000004	1
GACCGTC	20	0.0012556775	40.000004	25
GACGTAA	20	0.0012556775	40.000004	28
TAGCGGT	40	1.927765E-8	40.000004	22
ATAGCGG	40	1.927765E-8	40.000004	2
TCCTCGA	20	0.0012556775	40.000004	24
ATAGACG	20	0.0012556775	40.000004	1
TATGCGA	20	0.0012556775	40.000004	39
CGTTACA	20	0.0012556775	40.000004	33
ACTAGTA	40	1.927765E-8	40.000004	27
CTAACGC	20	0.0012556775	40.000004	28
TAACCGA	20	0.0012556775	40.000004	26
CGTAGTT	20	0.0012556775	40.000004	26
CGTAGAC	20	0.0012556775	40.000004	13
CTACGAC	20	0.0012556775	40.000004	37
CGCTAGA	40	1.927765E-8	40.000004	12
>>END_MODULE