##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246927
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85170111004467	31.0	31.0	34.0	30.0	34.0
2	32.02863194385385	33.0	31.0	34.0	30.0	34.0
3	32.171961753878676	33.0	31.0	34.0	30.0	34.0
4	36.01648260417046	37.0	35.0	37.0	35.0	37.0
5	35.68087329453644	37.0	35.0	37.0	35.0	37.0
6	35.65886679058993	37.0	35.0	37.0	33.0	37.0
7	35.444499791436336	37.0	35.0	37.0	33.0	37.0
8	35.70347106634755	37.0	35.0	37.0	35.0	37.0
9	37.20995273906863	39.0	37.0	39.0	34.0	39.0
10	36.64721962361346	39.0	37.0	39.0	32.0	39.0
11	36.83603251163299	39.0	37.0	39.0	33.0	39.0
12	36.50647762294119	38.0	35.0	39.0	32.0	39.0
13	36.24898451769146	38.0	35.0	39.0	32.0	39.0
14	37.420241609868505	39.0	36.0	41.0	32.0	41.0
15	37.52859752072475	39.0	36.0	40.0	33.0	41.0
16	37.5050116026194	39.0	35.0	40.0	33.0	41.0
17	37.43583326246218	39.0	35.0	40.0	33.0	41.0
18	37.20187747795907	38.0	35.0	40.0	33.0	41.0
19	37.073738392318376	39.0	35.0	40.0	32.0	41.0
20	37.0076783826799	39.0	35.0	40.0	32.0	41.0
21	36.997165154073876	39.0	35.0	40.0	32.0	41.0
22	36.976401932554964	39.0	35.0	40.0	32.0	41.0
23	36.86292709991212	38.0	35.0	40.0	32.0	41.0
24	36.50148829411121	38.0	35.0	40.0	31.0	41.0
25	36.55236162914546	38.0	35.0	40.0	31.0	41.0
26	36.444418795838445	38.0	35.0	40.0	31.0	41.0
27	36.287991997634926	38.0	35.0	40.0	31.0	41.0
28	36.2616076816225	38.0	35.0	40.0	31.0	41.0
29	36.07234931781458	38.0	35.0	40.0	30.0	41.0
30	36.0164542557112	38.0	35.0	40.0	30.0	41.0
31	35.88516849109251	38.0	35.0	40.0	30.0	41.0
32	35.81010582074864	38.0	35.0	40.0	30.0	41.0
33	35.71069992345916	38.0	35.0	40.0	30.0	41.0
34	35.572067858111915	38.0	35.0	40.0	29.0	41.0
35	35.36249579835336	38.0	34.0	40.0	28.0	41.0
36	35.25068542524714	38.0	34.0	40.0	27.0	41.0
37	35.06325756195151	38.0	34.0	40.0	26.0	41.0
38	34.89132415653209	37.0	34.0	40.0	26.0	41.0
39	34.79256622402573	37.0	34.0	40.0	25.0	41.0
40	34.50488605944267	37.0	34.0	40.0	23.0	41.0
41	34.488695039424606	37.0	34.0	40.0	23.0	41.0
42	34.30670198074735	37.0	33.0	40.0	23.0	41.0
43	34.26181827017702	37.0	33.0	40.0	23.0	41.0
44	34.12725218384381	36.0	33.0	40.0	23.0	41.0
45	33.92599432220859	36.0	33.0	40.0	23.0	41.0
46	33.840811251908455	36.0	33.0	40.0	22.0	41.0
47	33.702547716531605	35.0	33.0	40.0	22.0	41.0
48	33.54088860270444	35.0	33.0	40.0	20.0	41.0
49	33.3917190100718	35.0	33.0	39.0	20.0	41.0
50	33.22424441231619	35.0	33.0	39.0	20.0	41.0
51	31.22012173638363	35.0	29.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	8.0
13	6.0
14	15.0
15	24.0
16	48.0
17	94.0
18	194.0
19	428.0
20	699.0
21	1132.0
22	1603.0
23	2199.0
24	2577.0
25	2683.0
26	2815.0
27	3192.0
28	3658.0
29	4400.0
30	5238.0
31	6656.0
32	9084.0
33	13505.0
34	24455.0
35	25037.0
36	22533.0
37	33815.0
38	49654.0
39	31167.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.194928055660174	33.702673259708334	18.11993018179462	26.98246850283687
2	21.885820505655516	34.50128985489639	19.86943509620252	23.743454543245573
3	20.582601335617408	35.19582710679675	18.867114572322993	25.354456985262853
4	20.658332219643864	36.105407671093076	18.036504715968686	25.199755393294375
5	17.85588453267565	37.931858403495774	16.09301534461602	28.11924171921256
6	18.626962624581353	41.88808838239642	18.49615473399021	20.98879425903202
7	82.45189873930352	11.797818788548842	2.8887079987202693	2.8615744734273694
8	84.85017839280435	10.466656137238942	2.1957906587776956	2.4873748111790124
9	79.2424481729418	13.273153604101617	3.9991576457819518	3.485240577174631
10	40.4394821141471	34.771410173857056	10.137814009808567	14.651293702187287
11	31.780242743806873	32.80281216715871	19.62523336856642	15.791711720467994
12	30.54060511811183	29.048666204991758	24.90695630692472	15.503772369971692
13	23.37532955083891	35.8097737387973	23.37411461687057	17.44078209349322
14	18.68041971918826	38.52677106999234	25.212714688956655	17.580094521862737
15	18.172982298412084	32.54241131994476	30.640229703515615	18.644376678127543
16	19.79451416815496	29.310281986174054	31.573703969189275	19.321499876481713
17	19.886849149748713	28.694715442215717	25.06773256873489	26.350702839300684
18	22.078590028631943	32.620572071907894	24.63967083389018	20.661167065569984
19	22.243821048326023	34.557176817439974	22.752473403070546	20.44652873116346
20	25.28075099118363	32.18805557917927	22.53540520072734	19.99578822890976
21	22.52487577300174	32.12325910086787	24.591073475156623	20.76079165097377
22	22.530545464853986	30.294783478517946	23.96457252548324	23.210098531144833
23	21.923078480684573	32.482474577506714	23.39395853835344	22.200488403455275
24	21.285238147306693	31.61987145998615	27.565636807639503	19.52925358506765
25	20.699234996577935	31.81912063079372	25.54277175035537	21.938872622272978
26	21.35610929545979	35.601615052221916	22.64110445597282	20.401171196345476
27	20.071519112936212	35.01237207757759	24.491448889752842	20.42465991973336
28	19.110911321969652	34.77667488771986	26.703843646097837	19.40857014421266
29	19.03477545995375	32.043883414936396	26.95007026368117	21.971270861428682
30	21.050350913427856	32.60882771021395	25.95746921154835	20.383352164809843
31	24.460670562554927	32.3204833817282	22.284723825260098	20.934122230456776
32	23.568099073815336	32.8263008905466	24.035848651625784	19.569751384012278
33	23.43769616121364	32.9129661802881	22.532975332790663	21.1163623257076
34	22.502601983582192	33.39003025185581	23.572958809688693	20.534408954873303
35	20.544938382598907	32.985457240398986	26.023885601817543	20.445718775184567
36	24.806116787552597	31.204364042814277	24.851879300360025	19.137639869273105
37	23.210503509134277	32.91377613626699	23.880337103678414	19.995383250920312
38	22.47141867839483	31.250126555621705	23.732115159541078	22.546339606442388
39	23.27651492141402	30.430856082971893	24.580544047431022	21.712084948183065
40	24.008715126332884	30.400887711752866	24.5777092015049	21.01268796040935
41	21.191688231744603	29.038136777266153	27.45305292657344	22.3171220644158
42	23.21131346511317	29.906409586638965	24.480109506048347	22.402167442199517
43	23.439316073171423	29.869556589599355	24.580139069441575	22.110988267787647
44	21.69993560849968	31.60772212030276	25.485669853843447	21.206672417354117
45	21.957906587776954	33.258412405285775	23.766133310654567	21.01754769628271
46	23.463614752538202	30.040862279135126	27.234769790261897	19.26075317806477
47	22.886926095566707	30.630915209758346	26.11905542933742	20.36310326533753
48	24.34727672550997	29.125207044997104	26.422383943432674	20.105132286060254
49	22.385968322621665	28.301481814463386	27.54579288615664	21.766756976758312
50	22.121922673502695	31.80373146719476	25.03816917550531	21.036176683797237
51	22.07251535879025	30.141296820517805	25.9955371425563	21.79065067813564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7099.0
1	5487.0
2	3875.0
3	2545.0
4	1215.0
5	1114.0
6	1013.0
7	1041.0
8	1069.0
9	1149.0
10	1229.0
11	1254.5
12	1280.0
13	1284.5
14	1289.0
15	1284.0
16	1279.0
17	1190.5
18	1102.0
19	1128.0
20	1154.0
21	1139.0
22	1124.0
23	1183.0
24	1242.0
25	1427.5
26	2061.5
27	2510.0
28	2854.0
29	3198.0
30	3828.5
31	4459.0
32	5243.0
33	6027.0
34	6223.0
35	6419.0
36	6559.5
37	6700.0
38	7376.5
39	8053.0
40	9578.0
41	11103.0
42	12672.0
43	14241.0
44	15570.5
45	16900.0
46	17542.0
47	18184.0
48	17287.0
49	16390.0
50	16388.0
51	16386.0
52	15337.5
53	14289.0
54	13122.0
55	11955.0
56	11152.0
57	10349.0
58	9623.0
59	8897.0
60	9327.0
61	9757.0
62	9573.5
63	9390.0
64	8280.0
65	7170.0
66	6572.0
67	5974.0
68	5016.0
69	4058.0
70	3431.5
71	2805.0
72	2590.0
73	2375.0
74	1935.5
75	1214.0
76	932.0
77	720.0
78	508.0
79	387.0
80	266.0
81	223.0
82	180.0
83	166.0
84	152.0
85	142.0
86	132.0
87	87.0
88	42.0
89	30.0
90	18.0
91	19.5
92	21.0
93	12.0
94	3.0
95	3.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	246927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.341902667590016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81296343646126	19.972704483511322
2	6.205254410636666	3.1450590660397606
3	2.1222193812324215	1.6134323099539538
4	1.2848376374328816	1.3024092140592158
5	0.8805292763998978	1.1157143609244837
6	0.7063410892354897	1.0740016280115177
7	0.5401431858859627	0.9581779230298832
8	0.5017898235745334	1.0173047094890393
9	0.42827921247762724	0.9768069105444119
>10	7.39580669905395	45.50413685016219
>50	1.0403349526975199	16.784717750590257
>100	0.07510866785988238	2.734816362730686
>500	0.001598056762976221	0.29077419642242447
>1k	0.003196113525952442	1.046463124729171
>5k	0.001598056762976221	2.4634811098016822
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6083	2.4634811098016822	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1428	0.5783085689292787	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1156	0.4681545557998923	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	718	0.29077419642242447	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGT	458	0.1854799191663933	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCC	410	0.16604097567297216	No Hit
CTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGCT	288	0.1166336609605268	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17171066752522002	0.0
2	0.0	0.0	0.0	0.21949807027988028	0.0
3	0.0	0.0	0.0	0.3013036241480276	0.0
4	0.0	0.0	0.0	0.7188359312671356	0.0
5	0.0	0.0	0.0	0.7775577397368453	0.0
6	0.0	0.0	0.0	1.069951848117055	0.0
7	0.0	0.0	0.0	1.2854001385024725	0.0
8	0.0	0.0	0.0	1.4247125668719904	0.0
9	0.0	0.0	0.0	1.8880073867985274	0.0
10	0.0	0.0	0.0	2.2775962126458427	0.0
11	0.0	0.0	0.0	2.811357202736031	0.0
12	0.0	0.0	0.0	2.996837121902425	0.0
13	0.0	0.0	0.0	3.069733160002754	0.0
14	0.0	0.0	0.0	3.154778537786471	0.0
15	0.0	0.0	0.0	3.2143103022350736	0.0
16	0.0	0.0	0.0	3.327299161290584	0.0
17	0.0	0.0	0.0	3.457702073892284	0.0
18	0.0	0.0	0.0	3.652091508826495	0.0
19	0.0	0.0	0.0	3.7221527010007005	0.0
20	0.0	0.0	0.0	3.8112478586788807	0.0
21	0.0	0.0	0.0	3.9056077302198626	0.0
22	0.0	0.0	0.0	3.996727777845274	0.0
23	0.0	0.0	0.0	4.1129564608163545	0.0
24	0.0	0.0	0.0	4.184232586958899	0.0
25	0.0	0.0	0.0	4.256318669080335	0.0
26	0.0	0.0	0.0	4.320710169402293	0.0
27	0.0	0.0	0.0	4.383076779777019	0.0
28	0.0	0.0	0.0	4.449898148035654	0.0
29	0.0	0.0	0.0	4.5199593402098595	0.0
30	0.0	0.0	0.0	4.616344101698073	0.0
31	0.0	0.0	0.0	4.712728863186286	0.0
32	0.0	0.0	0.0	4.797369262980557	0.0
33	0.0	0.0	0.0	4.882009662774828	0.0
34	0.0	0.0	0.0	4.954905700875157	0.0
35	0.0	0.0	0.0	5.052100418342263	0.0
36	0.0	0.0	0.0	5.146865267872691	0.0
37	0.0	0.0	0.0	5.230290733698624	0.0
38	0.0	0.0	0.0	5.321410781324035	0.0
39	0.0	0.0	0.0	5.417795542812248	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAGG	30	2.1585565E-6	45.000004	2
GGTACGA	30	2.1585565E-6	45.000004	9
TATGGGC	70	0.0	45.000004	4
TTACTCG	30	2.1585565E-6	45.000004	1
TCGATCA	35	1.206754E-7	45.000004	17
CACTACG	35	1.206754E-7	45.000004	1
CTCGATC	35	1.206754E-7	45.000004	16
TTCCTCG	35	1.206754E-7	45.000004	12
TAATCAG	20	7.0225174E-4	45.0	1
CGGGTAT	25	3.8818813E-5	45.0	6
CTAGAAC	20	7.0225174E-4	45.0	20
GTCGCTA	20	7.0225174E-4	45.0	33
TCGCATG	20	7.0225174E-4	45.0	42
GTCGCAT	20	7.0225174E-4	45.0	41
GATAATC	20	7.0225174E-4	45.0	9
CTCGTCC	20	7.0225174E-4	45.0	37
TACTTGA	20	7.0225174E-4	45.0	42
GTCGAGG	20	7.0225174E-4	45.0	2
CCCTAGC	75	0.0	45.0	21
GCAACCT	25	3.8818813E-5	45.0	37
>>END_MODULE