##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548378_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1047617
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14868983607559	31.0	31.0	34.0	28.0	34.0
2	31.33575438351993	31.0	31.0	34.0	28.0	34.0
3	31.53623413900309	31.0	31.0	34.0	30.0	34.0
4	35.48599249534897	37.0	35.0	37.0	33.0	37.0
5	35.08374339095299	37.0	35.0	37.0	32.0	37.0
6	35.07531760175713	37.0	35.0	37.0	32.0	37.0
7	34.268263115241545	37.0	35.0	37.0	31.0	37.0
8	34.62179403350652	37.0	35.0	37.0	32.0	37.0
9	35.730444427686834	39.0	35.0	39.0	31.0	39.0
10	35.44228090991269	37.0	35.0	39.0	30.0	39.0
11	35.88135167718737	37.0	35.0	39.0	31.0	39.0
12	35.70626956225414	37.0	35.0	39.0	30.0	39.0
13	35.566084742802	37.0	35.0	39.0	30.0	39.0
14	36.67620609440282	38.0	35.0	40.0	31.0	41.0
15	36.7153692618581	38.0	35.0	40.0	31.0	41.0
16	36.701387052711056	38.0	35.0	40.0	31.0	41.0
17	36.62616586023327	38.0	35.0	40.0	31.0	41.0
18	36.432707754837885	38.0	35.0	40.0	31.0	41.0
19	36.38072788051358	38.0	35.0	40.0	30.0	41.0
20	36.327827822572566	38.0	35.0	40.0	30.0	41.0
21	36.300342587033235	38.0	34.0	40.0	30.0	41.0
22	36.28106073116415	38.0	35.0	40.0	30.0	41.0
23	36.144195827291846	38.0	34.0	40.0	30.0	41.0
24	35.777337519341515	38.0	34.0	40.0	29.0	41.0
25	35.858386223209436	38.0	34.0	40.0	30.0	41.0
26	35.688200936029105	38.0	34.0	40.0	29.0	41.0
27	35.53307363282574	38.0	34.0	40.0	28.0	41.0
28	35.46430136204357	38.0	34.0	40.0	28.0	41.0
29	35.24935735101664	37.0	34.0	40.0	27.0	41.0
30	35.23642418937455	37.0	34.0	40.0	27.0	41.0
31	35.08812094496366	37.0	34.0	40.0	27.0	41.0
32	35.02686859797044	37.0	34.0	40.0	26.0	41.0
33	34.93395678000643	37.0	33.0	40.0	26.0	41.0
34	34.861191637783655	37.0	34.0	40.0	26.0	41.0
35	34.7054753788837	37.0	33.0	40.0	25.0	41.0
36	34.53309940560338	37.0	33.0	40.0	24.0	41.0
37	34.399419826138754	37.0	33.0	40.0	24.0	41.0
38	34.23988919614707	37.0	33.0	40.0	24.0	41.0
39	34.12433551574669	37.0	33.0	40.0	23.0	41.0
40	33.88751423468691	36.0	33.0	40.0	23.0	41.0
41	33.87485884631502	36.0	33.0	40.0	23.0	41.0
42	33.78794635825879	36.0	33.0	40.0	22.0	41.0
43	33.726200510301	36.0	33.0	40.0	22.0	41.0
44	33.593952751816744	36.0	33.0	39.0	22.0	41.0
45	33.36969235894416	35.0	32.0	39.0	22.0	41.0
46	33.22673171588472	35.0	32.0	39.0	21.0	40.0
47	33.083844572968935	35.0	32.0	39.0	20.0	40.0
48	32.94455511890319	35.0	32.0	39.0	20.0	40.0
49	32.754943839208416	35.0	31.0	39.0	20.0	40.0
50	32.58472418832455	35.0	31.0	39.0	19.0	40.0
51	30.68569047657684	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	18.0
10	19.0
11	23.0
12	36.0
13	48.0
14	95.0
15	210.0
16	414.0
17	817.0
18	1614.0
19	2656.0
20	4197.0
21	5978.0
22	7979.0
23	10043.0
24	11975.0
25	13660.0
26	15414.0
27	17833.0
28	21490.0
29	26266.0
30	32148.0
31	40326.0
32	51872.0
33	69514.0
34	104580.0
35	107906.0
36	110212.0
37	140084.0
38	163324.0
39	86853.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.799299744085864	32.86095968278484	19.69183394312998	26.647906629999323
2	23.345363811392904	33.02666909758051	19.294933167369372	24.333033923657215
3	20.264275971084853	34.749245191706514	19.39926518947287	25.58721364773577
4	20.92224543893427	34.68910871053066	17.776916563973284	26.611729286561786
5	18.08055806654531	37.08998613042744	16.144927010539156	28.684528792488095
6	19.00675533138542	39.11372190409281	18.71752749334919	23.161995271172575
7	80.92289453111204	12.176873800253336	3.5640887843553513	3.3361428842792735
8	84.42789683634382	9.62603699634504	2.779450887108552	3.166615280202593
9	78.22276652631639	13.908231729725653	4.664109116213273	3.204892627744681
10	40.33955157275989	36.76524913207785	9.979219504838124	12.915979790324137
11	33.46719268587661	30.663878115761772	19.32137412814034	16.54755507022127
12	30.324918362340437	28.216609696100768	24.686693705810427	16.771778235748368
13	23.771187370957133	35.07426855425217	21.671183266403656	19.483360808387033
14	18.96656888920283	35.54982402920151	26.23468309506241	19.248923986533246
15	18.713900213532238	31.799693972129127	30.238340920393618	19.24806489394502
16	20.871845340424983	29.884108409848253	28.9463611224331	20.297685127293658
17	20.260553236535873	29.25343899535804	25.193653787596038	25.29235398051005
18	23.3889866239284	30.047813275271402	23.57015970531215	22.993040395488045
19	23.695300858997133	32.740782175165165	22.324379997651814	21.23953696818589
20	28.621624124083517	30.207509041949493	21.486860178863076	19.684006655103918
21	23.81920110116579	31.459206942995387	23.369132039667168	21.352459916171654
22	23.44377764011084	29.410843848467522	21.803769889186604	25.341608622235036
23	22.373730094108822	34.188162276862634	20.455758163527317	22.98234946550123
24	21.20135507537583	31.272688396618232	26.433992575530947	21.091963952474998
25	22.053861287092516	30.643450803108387	22.826090069176043	24.47659784062305
26	23.35099564058239	34.39062176348799	20.69038589484516	21.56799670108446
27	20.338348843136377	33.6879794810508	22.80489911866646	23.16877255714636
28	20.57918113203585	33.48046089362811	25.031285288421245	20.909072685914794
29	20.944963665156255	31.73125292926709	24.33847484338265	22.985308562194007
30	23.12476792568276	31.03729702744419	24.189565461423403	21.648369585449643
31	25.780604934818736	31.267820205284945	21.259009733519026	21.692565126377293
32	25.183631040733395	30.158636219152612	23.73338729707517	20.92434544303882
33	23.91303310274652	31.06574253758769	21.596633120692008	23.424591238973786
34	22.708585294053073	31.319747579506636	24.07921979120232	21.892447335237975
35	21.495164740549267	31.46121149236792	24.671898222346524	22.371725544736293
36	24.921416891860286	31.473525152799166	22.311111789900316	21.293946165440232
37	23.408650298725583	31.896866889330738	22.706198925752446	21.988283886191233
38	23.592114293677938	31.050469780463665	22.823512791411364	22.533903134447037
39	23.943864981190647	28.290778022884318	24.23003826780207	23.53531872812297
40	24.681252786084993	29.185666135620174	23.749137327859323	22.383943750435513
41	21.69141966959299	27.711654163687683	25.46884978002457	25.128076386694755
42	24.95110331352011	28.664387844030788	22.224534347953497	24.159974494495604
43	23.93804224253711	28.710397024866914	23.44912310510425	23.902437627491725
44	22.840121914783744	29.45169847377429	24.09181981582964	23.616359795612325
45	23.06510871816704	30.70960093240182	22.573135029309373	23.65215532012176
46	23.46668677579688	27.903040901398125	25.889614238791463	22.74065808401353
47	23.403782107392303	30.318809259490827	24.63018450445153	21.647224128665343
48	24.49721606274049	27.051107418073588	26.26513315457844	22.186543364607488
49	23.017476806886485	27.344821628515003	26.16872387523303	23.468977689365484
50	22.07743860590273	29.412657488376	24.71027102462064	23.79963288110063
51	22.29354811920769	28.930324727452877	24.72697560272504	24.049151550614393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19403.0
1	15377.5
2	11352.0
3	7817.5
4	4283.0
5	3764.5
6	3246.0
7	3382.5
8	3519.0
9	3676.0
10	3833.0
11	4024.0
12	4215.0
13	4285.5
14	4356.0
15	4417.5
16	4479.0
17	4577.5
18	4676.0
19	4366.0
20	4056.0
21	4259.5
22	4463.0
23	4017.0
24	3571.0
25	4723.0
26	8098.0
27	10321.0
28	11503.0
29	12685.0
30	14017.5
31	15350.0
32	17852.5
33	20355.0
34	22558.0
35	24761.0
36	27786.0
37	30811.0
38	32976.5
39	35142.0
40	38975.5
41	42809.0
42	48524.5
43	54240.0
44	63406.5
45	72573.0
46	72643.5
47	72714.0
48	69886.5
49	67059.0
50	67362.0
51	67665.0
52	67204.0
53	66743.0
54	61050.5
55	55358.0
56	51869.0
57	48380.0
58	48107.5
59	47835.0
60	47989.5
61	48144.0
62	46931.5
63	45719.0
64	41464.0
65	37209.0
66	30922.5
67	24636.0
68	21070.5
69	17505.0
70	16005.0
71	14505.0
72	13528.0
73	12551.0
74	10506.5
75	7174.0
76	5886.0
77	4052.5
78	2219.0
79	2224.5
80	2230.0
81	1576.5
82	923.0
83	775.5
84	628.0
85	508.5
86	389.0
87	306.0
88	223.0
89	185.5
90	148.0
91	102.5
92	57.0
93	41.5
94	26.0
95	21.0
96	16.0
97	12.5
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1047617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.56928772454845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.74304662058445	19.030560975571838
2	8.38938520567476	3.9546366748923694
3	2.9673959177653084	2.0981822453538315
4	1.466139687287606	1.382234725362443
5	0.8608284398416657	1.0144556590051181
6	0.5565362375915989	0.7870297627760429
7	0.3840375812880085	0.6336044575291715
8	0.2793870500598245	0.5267963015498259
9	0.2109295666301249	0.44743136809677553
>10	2.206404446059838	12.055480236379138
>50	0.9107732564541594	15.779854334773374
>100	1.0172916154809457	38.28743058326874
>500	0.004541480426254222	0.7014003813416247
>1k	0.002890032998525414	1.7089336150279708
>5k	0.0	0.0
>10k+	4.1286185693220205E-4	1.5919686790717233
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16360	1.5616394159315856	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3837	0.36625980678053144	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3399	0.3244506341535122	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2234	0.2132458713442031	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGT	2081	0.19864129734435393	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	2035	0.19425037967119663	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	2015	0.19234128503069348	Illumina PCR Primer Index 10 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	1961	0.18718672950133494	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.909094640503161E-4	0.0	0.0	0.30192331739557493	0.0
2	1.909094640503161E-4	0.0	0.0	0.37733255569544977	0.0
3	1.909094640503161E-4	0.0	0.0	0.502473709380432	0.0
4	1.909094640503161E-4	0.0	0.0	1.2288842200918848	0.0
5	1.909094640503161E-4	0.0	0.0	1.3273935035418478	0.0
6	1.909094640503161E-4	0.0	0.0	1.812494451693701	0.0
7	1.909094640503161E-4	0.0	0.0	2.186963365428396	0.0
8	1.909094640503161E-4	0.0	0.0	2.398204687400071	0.0
9	1.909094640503161E-4	0.0	0.0	2.978092184452906	0.0
10	1.909094640503161E-4	0.0	0.0	3.4686340523301933	0.0
11	1.909094640503161E-4	0.0	0.0	4.14922629166957	0.0
12	1.909094640503161E-4	0.0	0.0	4.4114404405426795	0.0
13	1.909094640503161E-4	0.0	0.0	4.546413431626252	0.0
14	1.909094640503161E-4	0.0	0.0	4.667831850762254	0.0
15	1.909094640503161E-4	0.0	0.0	4.757463844133877	0.0
16	1.909094640503161E-4	0.0	0.0	4.918591431792344	0.0
17	1.909094640503161E-4	0.0	0.0	5.129260025371868	0.0
18	1.909094640503161E-4	0.0	0.0	5.401401466375593	0.0
19	1.909094640503161E-4	0.0	0.0	5.511460772400601	0.0
20	1.909094640503161E-4	0.0	0.0	5.635074650373181	0.0
21	1.909094640503161E-4	0.0	0.0	5.772338555025358	0.0
22	1.909094640503161E-4	0.0	0.0	5.917811566631698	0.0
23	1.909094640503161E-4	0.0	0.0	6.065384582342593	0.0
24	1.909094640503161E-4	0.0	0.0	6.172198427478745	0.0
25	1.909094640503161E-4	0.0	0.0	6.267653159503903	0.0
26	1.909094640503161E-4	0.0	0.0	6.352416961542243	0.0
27	1.909094640503161E-4	0.0	0.0	6.448635331423603	0.0
28	1.909094640503161E-4	0.0	0.0	6.542085514076232	0.0
29	1.909094640503161E-4	0.0	0.0	6.645367534127453	0.0
30	2.8636419607547416E-4	0.0	0.0	6.793226914034423	0.0
31	2.8636419607547416E-4	0.0	0.0	6.899086211850324	0.0
32	2.8636419607547416E-4	0.0	0.0	7.008572789483179	0.0
33	2.8636419607547416E-4	0.0	0.0	7.1195866428284384	0.0
34	2.8636419607547416E-4	0.0	0.0	7.227450490016867	0.0
35	2.8636419607547416E-4	0.0	0.0	7.361278024316139	0.0
36	2.8636419607547416E-4	0.0	0.0	7.468282778916341	0.0
37	2.8636419607547416E-4	0.0	0.0	7.587983012875889	0.0
38	2.8636419607547416E-4	0.0	0.0	7.701383234521777	0.0
39	2.8636419607547416E-4	0.0	0.0	7.832251672128268	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTC	20	7.0329994E-4	45.000004	23
TAGCGTA	20	7.0329994E-4	45.000004	34
ATTCGGA	20	7.0329994E-4	45.000004	33
TCGATTG	20	7.0329994E-4	45.000004	1
TACGGGT	150	0.0	45.000004	4
ACGGGTA	150	0.0	45.000004	5
TCGCTTA	20	7.0329994E-4	45.000004	27
TCGCTAG	20	7.0329994E-4	45.000004	14
GAACGAT	20	7.0329994E-4	45.000004	34
GAACGAC	20	7.0329994E-4	45.000004	17
CCGGTAT	20	7.0329994E-4	45.000004	37
CACGTAT	20	7.0329994E-4	45.000004	39
CGACTAT	20	7.0329994E-4	45.000004	21
CTCTCGT	20	7.0329994E-4	45.000004	29
CAATACG	75	0.0	45.000004	1
TAGTACG	40	6.8139343E-9	45.000004	1
TAATCGC	20	7.0329994E-4	45.000004	15
TAATCCG	20	7.0329994E-4	45.000004	1
TCACGGT	25	3.8905684E-5	45.0	40
CGTGGTA	45	3.8562575E-10	45.0	26
>>END_MODULE