FastQCFastQC Report
Sat 18 Jun 2016
SRR3548377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127437
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212901.8883538503703532No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53440.47399544276088157No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43450.38538738749925716No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT37450.33216933629107437No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29530.26192150869627306No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTAT20790.18440054743635342No Hit
AATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGT17300.15344538098359375No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGT17060.15131665893526647No Hit
AAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGT11980.10625870891233834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCA451.2223609E-940.019
TGCCCGT200.001255758540.032
CGTATCT200.001255758540.031
GTCGACA353.0563024E-740.022
ACGTTAG353.0563024E-740.01
CCGGCAT257.7945595E-540.030
TAGCGTA257.7945595E-540.034
ATTACGA353.0563024E-740.019
GCTATCG257.7945595E-540.01
ATAGCGT200.001255758540.015
CGTCTCC353.0563024E-740.035
CGTCTAC200.001255758540.039
TGCGGTC401.9283107E-840.028
TACGGGT1150.040.04
AGGAACG257.7945595E-540.01
CGCGACT353.0563024E-740.027
TGTGCGA257.7945595E-540.033
TCTAGCG200.001255758540.01
TAGTTTC304.867432E-640.023
CGTTAGA200.001255758540.05