##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548377_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1127437
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.012183385856595	31.0	31.0	34.0	28.0	34.0
2	31.34645394820287	31.0	31.0	34.0	28.0	34.0
3	31.513439775348868	31.0	31.0	34.0	28.0	34.0
4	35.482726751029105	37.0	35.0	37.0	33.0	37.0
5	35.06550166439455	37.0	35.0	37.0	32.0	37.0
6	35.219866830696525	37.0	35.0	37.0	32.0	37.0
7	35.1532165433634	37.0	35.0	37.0	32.0	37.0
8	35.584313802012886	37.0	35.0	37.0	35.0	37.0
9	36.896777380909086	39.0	37.0	39.0	32.0	39.0
10	36.160682148980385	37.0	35.0	39.0	31.0	39.0
11	36.390499868285325	38.0	35.0	39.0	32.0	39.0
12	36.118380894010045	37.0	35.0	39.0	32.0	39.0
13	35.84570933896972	38.0	35.0	39.0	30.0	39.0
14	37.04114642325913	39.0	35.0	40.0	31.0	41.0
15	36.94641385727096	39.0	35.0	40.0	32.0	41.0
16	36.857478510994405	38.0	35.0	40.0	31.0	41.0
17	36.81442333363194	38.0	35.0	40.0	32.0	41.0
18	36.647033936264286	38.0	35.0	40.0	31.0	41.0
19	36.8268648270369	38.0	35.0	40.0	31.0	41.0
20	36.611835517195196	38.0	35.0	40.0	31.0	41.0
21	36.6692081242677	38.0	35.0	40.0	31.0	41.0
22	36.75782771010709	38.0	35.0	40.0	31.0	41.0
23	36.551581152649774	38.0	35.0	40.0	31.0	41.0
24	36.148436675397384	38.0	35.0	40.0	30.0	41.0
25	36.34916008610681	38.0	35.0	40.0	31.0	41.0
26	36.33201056910497	38.0	35.0	40.0	31.0	41.0
27	36.215291852227665	38.0	35.0	40.0	30.0	41.0
28	36.2226536826448	38.0	35.0	40.0	30.0	41.0
29	35.93959662491119	38.0	35.0	40.0	30.0	41.0
30	35.94427803948247	38.0	35.0	40.0	30.0	41.0
31	35.89844576681447	38.0	35.0	40.0	30.0	41.0
32	35.87360712838056	38.0	35.0	40.0	30.0	41.0
33	35.824581772640066	38.0	35.0	40.0	30.0	41.0
34	35.74466422514074	38.0	35.0	40.0	29.0	41.0
35	35.523725937679885	38.0	34.0	40.0	29.0	41.0
36	35.46272474648251	38.0	34.0	40.0	28.0	41.0
37	35.347858017787246	38.0	34.0	40.0	28.0	41.0
38	35.132206943713925	37.0	34.0	40.0	27.0	41.0
39	35.084829573625846	37.0	34.0	40.0	27.0	41.0
40	34.86377864128993	37.0	34.0	40.0	26.0	41.0
41	34.91956180256635	37.0	34.0	40.0	26.0	41.0
42	34.800357802697626	37.0	34.0	40.0	26.0	41.0
43	34.754917569673516	37.0	34.0	40.0	26.0	41.0
44	34.6225172670402	37.0	34.0	40.0	25.0	41.0
45	34.389656362173675	36.0	33.0	40.0	24.0	41.0
46	33.332040726000656	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	9.0
10	31.0
11	18.0
12	23.0
13	37.0
14	36.0
15	87.0
16	179.0
17	338.0
18	652.0
19	1254.0
20	2064.0
21	3294.0
22	5030.0
23	6784.0
24	8701.0
25	10797.0
26	12591.0
27	14556.0
28	17242.0
29	21350.0
30	26625.0
31	34735.0
32	46021.0
33	64776.0
34	105646.0
35	118702.0
36	111925.0
37	152697.0
38	214653.0
39	146577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.724528288498604	32.146629922558866	19.61466583055195	27.51417595839058
2	23.72717943441629	32.302381419094814	18.694969208922537	25.27546993756636
3	20.11296418336457	34.60299777282456	19.20284681095263	26.08119123285824
4	20.344994886632247	34.878312491074894	17.900512401136382	26.87618022115648
5	18.44830354157261	36.56718734616657	16.207468798700063	28.777040313560757
6	19.16071585374615	38.72207493633791	18.762910920964984	23.354298288950957
7	82.25878696548011	10.354015346312034	3.9096641320091496	3.477533556198706
8	84.74220732510996	8.863289035218818	3.0416777168036884	3.3528259228675306
9	79.34740477738445	12.129990411881106	5.1620622704417185	3.3605425402927174
10	40.237104157482854	36.30641889524647	10.184604549965986	13.271872397304682
11	32.915808156021136	30.537404750775433	19.672318719360817	16.874468373842618
12	30.14163984329058	28.092656175023528	24.787371711235306	16.97833227045059
13	23.863949826021322	34.7763112262592	22.067929294497166	19.29180965322231
14	18.931612143294924	35.24436398663517	26.218848591983413	19.60517527808649
15	18.65966790162111	31.565577500117527	30.182529045968863	19.5922255522925
16	20.643725547414178	30.18261774272088	28.701736771101178	20.471919938763765
17	20.114738118404844	29.108588772587733	25.509274575874308	25.267398533133118
18	23.307022920127686	29.54887944958344	23.739951766706255	23.40414586358262
19	23.567347887287717	32.73140760858478	22.484449241953207	21.216795262174294
20	28.216654234338595	30.101637608132425	21.885568772357125	19.796139385171855
21	23.58499854093843	31.575422839591038	23.655335065285243	21.18424355418529
22	23.2940731943337	29.40394895679315	22.008679864152057	25.293297984721097
23	22.159641736079266	34.23135838188741	20.510591722641706	23.09840815939161
24	20.959840771590784	31.13761567165172	26.533544668127796	21.368998888629697
25	21.774609135588065	30.903722336591755	22.942479269351633	24.379189258468546
26	23.216552233073777	34.109134257612624	21.02183980124832	21.652473708065283
27	20.179486747374796	33.75683075861445	22.662818410252637	23.400864083758115
28	20.31678931949191	33.44594864280665	25.219768377301794	21.01749366039965
29	20.827239127330397	31.934023807982175	24.33741308827012	22.901323976417306
30	22.910104954866657	31.101604790334182	24.12471827694142	21.86357197785774
31	25.498630965632668	31.147993191637312	21.453792983554735	21.89958285917528
32	25.01638672493452	30.20142145414777	23.791129792618122	20.991062028299588
33	23.504461890110047	31.01051322601618	21.89568020208668	23.589344681787097
34	22.545650000842617	31.177883997065912	24.344331434927184	21.932134567164287
35	21.317909559469843	31.43971680901017	24.689627890516277	22.55274574100371
36	24.531392884923946	31.562473113797047	22.52516105112747	21.380972950151538
37	23.04084396733476	31.824749409501372	22.865756578859838	22.268650044304028
38	23.303031566287075	31.100451732558003	23.213093059745248	22.383423641409674
39	23.52406387230506	28.392096409821573	24.410853998937412	23.67298571893596
40	24.515782256569548	29.121095014621663	23.744297907554923	22.61882482125387
41	21.547988934193217	27.590721255378348	25.57420059834829	25.287089212080144
42	24.780453364578243	28.685771355738726	22.444801793803112	24.08897348587992
43	23.44867163309347	28.652421376981597	23.792726334154366	24.10618065577057
44	22.70219976814669	29.396587126376016	24.182193772246254	23.719019333231035
45	22.763311830284085	30.359124279228016	23.1802752615002	23.697288628987696
46	23.214689601281492	27.819026695061456	26.107179381198243	22.859104322458816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25423.0
1	20253.0
2	15083.0
3	10327.0
4	5571.0
5	5026.5
6	4482.0
7	4482.0
8	4472.0
9	4462.0
10	4459.0
11	4456.0
12	4366.0
13	4276.0
14	4268.5
15	4261.0
16	4439.0
17	4617.0
18	4576.5
19	4536.0
20	4536.0
21	4409.5
22	4283.0
23	4340.5
24	4398.0
25	5946.5
26	7495.0
27	9793.0
28	12091.0
29	13940.5
30	15790.0
31	15790.0
32	16112.0
33	16434.0
34	21932.5
35	27431.0
36	29093.0
37	30755.0
38	35063.5
39	39372.0
40	44768.0
41	50164.0
42	58794.5
43	67425.0
44	67425.0
45	75671.5
46	83918.0
47	87826.5
48	91735.0
49	87930.5
50	84126.0
51	80644.0
52	77162.0
53	73117.0
54	69072.0
55	64938.0
56	60804.0
57	60804.0
58	58122.5
59	55441.0
60	56199.0
61	56957.0
62	55106.5
63	53256.0
64	48897.5
65	44539.0
66	37515.0
67	30491.0
68	25889.0
69	21287.0
70	21287.0
71	19603.5
72	17920.0
73	14483.5
74	11047.0
75	8938.0
76	6829.0
77	6079.5
78	5330.0
79	4150.5
80	2971.0
81	2971.0
82	2005.5
83	1040.0
84	669.5
85	299.0
86	233.0
87	167.0
88	155.0
89	143.0
90	109.5
91	76.0
92	42.0
93	8.0
94	8.0
95	10.0
96	12.0
97	6.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1127437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.04964583755583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10058086071106	13.9163817840567
2	9.728400416718777	3.511883641754089
3	3.4003286810279656	1.8412418527151437
4	1.687262951896152	1.2181799486621816
5	0.9666858232493873	0.8724168372918768
6	0.6767432199880961	0.732898526625136
7	0.4642594922459865	0.5865803588234487
8	0.3218522292268916	0.4647454999658583
9	0.288274465857949	0.4682926811471904
>10	2.7672587414136487	11.562308226750654
>50	1.0797193873059863	14.320424201766215
>100	1.5092319575080269	45.88464480210301
>500	0.004453471350023686	0.5657217773548834
>1k	0.003958641200021054	1.6754617652984305
>5k	4.948301500026318E-4	0.4772998386776913
>10k+	4.948301500026318E-4	1.901518257007494
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21290	1.8883538503703532	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5344	0.47399544276088157	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4345	0.38538738749925716	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT	3745	0.33216933629107437	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2953	0.26192150869627306	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTAT	2079	0.18440054743635342	No Hit
AATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGT	1730	0.15344538098359375	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGT	1706	0.15131665893526647	No Hit
AAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGT	1198	0.10625870891233834	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.869675201363801E-5	0.0	0.0	0.385919568011339	0.0
2	8.869675201363801E-5	0.0	0.0	0.5028218871653138	0.0
3	8.869675201363801E-5	0.0	0.0	0.6589281707093168	0.0
4	8.869675201363801E-5	0.0	0.0	1.428993371691722	0.0
5	8.869675201363801E-5	0.0	0.0	1.5486452901581198	0.0
6	8.869675201363801E-5	0.0	0.0	2.1511623265867628	0.0
7	8.869675201363801E-5	0.0	0.0	2.581873754364989	0.0
8	8.869675201363801E-5	0.0	0.0	2.8387395481964846	0.0
9	1.7739350402727603E-4	0.0	0.0	3.38280542504814	0.0
10	1.7739350402727603E-4	0.0	0.0	3.9945469236862015	0.0
11	1.7739350402727603E-4	0.0	0.0	4.729842997879261	0.0
12	1.7739350402727603E-4	0.0	0.0	5.06165754716228	0.0
13	1.7739350402727603E-4	0.0	0.0	5.214038567121711	0.0
14	1.7739350402727603E-4	0.0	0.0	5.354800312567354	0.0
15	1.7739350402727603E-4	0.0	0.0	5.45635809362297	0.0
16	1.7739350402727603E-4	0.0	0.0	5.659296262230173	0.0
17	1.7739350402727603E-4	0.0	0.0	5.9119046119650145	0.0
18	1.7739350402727603E-4	0.0	0.0	6.250814901409125	0.0
19	1.7739350402727603E-4	0.0	0.0	6.38989140856651	0.0
20	2.66090256040914E-4	0.0	0.0	6.53473320460478	0.0
21	2.66090256040914E-4	0.0	0.0	6.70343442693472	0.0
22	2.66090256040914E-4	0.0	0.0	6.877723544641519	0.0
23	2.66090256040914E-4	0.0	0.0	7.059640583021491	0.0
24	2.66090256040914E-4	0.0	0.0	7.188428266945293	0.0
25	2.66090256040914E-4	0.0	0.0	7.304975799091213	0.0
26	2.66090256040914E-4	0.0	0.0	7.41753197739652	0.0
27	2.66090256040914E-4	0.0	0.0	7.530797729717936	0.0
28	2.66090256040914E-4	0.0	0.0	7.644063482039352	0.0
29	2.66090256040914E-4	0.0	0.0	7.767440664090322	0.0
30	2.66090256040914E-4	0.0	0.0	7.95122033426258	0.0
31	2.66090256040914E-4	0.0	0.0	8.080806288954506	0.0
32	2.66090256040914E-4	0.0	0.0	8.20648958655783	0.0
33	2.66090256040914E-4	0.0	0.0	8.34201822363467	0.0
34	2.66090256040914E-4	0.0	0.0	8.474797261399084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCA	45	1.2223609E-9	40.0	19
TGCCCGT	20	0.0012557585	40.0	32
CGTATCT	20	0.0012557585	40.0	31
GTCGACA	35	3.0563024E-7	40.0	22
ACGTTAG	35	3.0563024E-7	40.0	1
CCGGCAT	25	7.7945595E-5	40.0	30
TAGCGTA	25	7.7945595E-5	40.0	34
ATTACGA	35	3.0563024E-7	40.0	19
GCTATCG	25	7.7945595E-5	40.0	1
ATAGCGT	20	0.0012557585	40.0	15
CGTCTCC	35	3.0563024E-7	40.0	35
CGTCTAC	20	0.0012557585	40.0	39
TGCGGTC	40	1.9283107E-8	40.0	28
TACGGGT	115	0.0	40.0	4
AGGAACG	25	7.7945595E-5	40.0	1
CGCGACT	35	3.0563024E-7	40.0	27
TGTGCGA	25	7.7945595E-5	40.0	33
TCTAGCG	20	0.0012557585	40.0	1
TAGTTTC	30	4.867432E-6	40.0	23
CGTTAGA	20	0.0012557585	40.0	5
>>END_MODULE