FastQCFastQC Report
Sat 18 Jun 2016
SRR3548376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454712
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205554.520443709424867No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49271.0835429898485194No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41610.915084712961171No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC30210.6643765724238639No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGT28340.6232516405988845No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24610.5412216963704499No Hit
AATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT22010.48404264677422193No Hit
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT21690.47700522528545547TruSeq Adapter, Index 16 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT6200.13635004134485126No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6090.13393092770808776No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCT4880.10732067770368937No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC207.028783E-445.00000419
TCACGCG207.028783E-445.0000041
TATTTCG207.028783E-445.00000427
AGACTCG207.028783E-445.0000041
GTACCAC207.028783E-445.00000423
CGTATAA207.028783E-445.00000420
GGTCGGT207.028783E-445.00000412
CAAGTAC207.028783E-445.00000424
CTCCGAG207.028783E-445.00000430
GGTCGAG207.028783E-445.00000418
CACGGCC207.028783E-445.00000420
AGATACG207.028783E-445.0000041
CGAAACG207.028783E-445.00000414
TCCGATC207.028783E-445.00000414
TAGACGA207.028783E-445.00000412
GCGCGAC207.028783E-445.0000049
CCGGCTA207.028783E-445.00000430
ACCGCAA207.028783E-445.00000429
GACAATC207.028783E-445.00000445
CGGTTCG207.028783E-445.00000415