##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548376_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	454712
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99333424233361	31.0	31.0	33.0	28.0	34.0
2	31.14786722145006	31.0	31.0	34.0	28.0	34.0
3	31.41842968736255	31.0	31.0	34.0	30.0	34.0
4	35.371030454441495	37.0	35.0	37.0	33.0	37.0
5	34.8898203698165	35.0	35.0	37.0	32.0	37.0
6	34.85952207111315	35.0	35.0	37.0	32.0	37.0
7	33.84765521912771	35.0	35.0	37.0	30.0	37.0
8	34.25535943630254	35.0	35.0	37.0	31.0	37.0
9	35.340655183940605	38.0	35.0	39.0	30.0	39.0
10	35.2309373845423	37.0	34.0	39.0	29.0	39.0
11	35.62244893471032	37.0	35.0	39.0	30.0	39.0
12	35.379284030331284	37.0	35.0	39.0	30.0	39.0
13	35.128067875930256	37.0	34.0	39.0	30.0	39.0
14	36.13837330002287	38.0	35.0	40.0	30.0	41.0
15	36.324928746107425	38.0	35.0	40.0	31.0	41.0
16	36.26544274177941	38.0	34.0	40.0	31.0	41.0
17	36.173505427611325	38.0	35.0	40.0	31.0	41.0
18	35.75153283836802	37.0	34.0	40.0	30.0	41.0
19	35.4463902426151	36.0	34.0	40.0	29.0	41.0
20	35.28275040025335	36.0	33.0	40.0	29.0	41.0
21	35.308432590298914	36.0	33.0	40.0	29.0	41.0
22	35.254334611798235	36.0	33.0	40.0	29.0	41.0
23	34.98692139200197	36.0	33.0	40.0	27.0	41.0
24	34.52787258748395	35.0	33.0	39.0	25.0	41.0
25	34.601459825120074	35.0	33.0	39.0	25.0	41.0
26	34.46790495962279	35.0	33.0	39.0	25.0	41.0
27	34.317095216312744	35.0	33.0	39.0	24.0	41.0
28	34.1782644838931	35.0	33.0	39.0	24.0	40.0
29	33.91975580147434	35.0	33.0	39.0	23.0	40.0
30	33.868156107602175	35.0	33.0	39.0	23.0	40.0
31	33.523456605499746	35.0	33.0	39.0	22.0	40.0
32	33.31002480691075	35.0	32.0	39.0	21.0	40.0
33	33.06503017294463	35.0	32.0	39.0	20.0	40.0
34	32.903921603124616	35.0	32.0	39.0	19.0	40.0
35	32.625600380020764	35.0	31.0	39.0	18.0	40.0
36	32.41132848924154	35.0	31.0	39.0	16.0	40.0
37	32.20448987490983	35.0	31.0	39.0	15.0	40.0
38	32.079898925033866	35.0	31.0	39.0	15.0	40.0
39	31.852902496525275	35.0	30.0	39.0	15.0	40.0
40	31.58765768222523	35.0	30.0	39.0	12.0	40.0
41	31.51169311564243	35.0	30.0	39.0	10.0	40.0
42	31.31501257939091	35.0	30.0	39.0	10.0	40.0
43	31.151625644363904	35.0	30.0	39.0	10.0	40.0
44	31.059365048646175	35.0	29.0	39.0	10.0	40.0
45	30.837015517514384	35.0	29.0	38.0	9.0	40.0
46	30.67834365488485	35.0	28.0	38.0	9.0	40.0
47	30.529818874364434	35.0	27.0	38.0	8.0	40.0
48	30.318652245817134	35.0	27.0	38.0	8.0	40.0
49	30.152065483206954	35.0	27.0	38.0	8.0	40.0
50	29.89483673182146	34.0	26.0	38.0	8.0	40.0
51	28.116427540948997	33.0	22.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	8.0
11	9.0
12	14.0
13	17.0
14	42.0
15	107.0
16	269.0
17	606.0
18	1245.0
19	2496.0
20	3893.0
21	5715.0
22	7987.0
23	9360.0
24	10050.0
25	10252.0
26	10356.0
27	10966.0
28	12090.0
29	13529.0
30	16139.0
31	20029.0
32	24817.0
33	33091.0
34	49240.0
35	50639.0
36	40987.0
37	47673.0
38	49982.0
39	23099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.32280652368972	42.28918524252714	17.453684969827055	21.934323263956088
2	16.72245289325991	46.09291155720544	18.008321750910465	19.176313798624182
3	16.511990006861485	44.68806629251043	18.39735920758634	20.40258449304175
4	17.171088513168776	44.78593043508858	15.460115413712415	22.582865638030224
5	13.413545277010503	49.94875877478492	14.946603564453984	21.691092383750593
6	15.497281795949963	47.455312373546334	17.654471401678425	19.392934428825278
7	64.89888104998329	26.822472246169006	4.318337761044353	3.9603089428033567
8	68.19239430672602	23.36247998733264	3.2431517092137443	5.201973996727599
9	63.46060803321662	27.238119952849278	5.837321205510301	3.4639508084237933
10	34.40331462552121	46.97412867925192	8.841860342370556	9.780696352856314
11	26.640818803990218	38.50085328735551	21.940261088337234	12.918066820317037
12	26.40242615105825	37.36386987807667	22.831374584352293	13.402329386512783
13	18.388562430725383	47.87579830750013	19.023909639508084	14.7117296222664
14	12.662520452506202	49.334743749890045	23.288367142279068	14.714368655324689
15	11.364775946093351	42.74727739756153	29.9651207797463	15.922825876598814
16	12.783476134344376	41.1576998187864	29.66537060820915	16.393453438660075
17	13.836670244022589	40.39216031246151	22.058357817695594	23.712811625820297
18	16.74642411020602	43.007442073224375	22.039884586287585	18.206249230282022
19	16.653178275479863	45.09953553018174	22.03570611727863	16.211580077059764
20	19.224036313094885	44.265381164341385	19.372042083780503	17.138540438783227
21	14.556686430092014	45.052032935132566	21.668000844490578	18.72327979028484
22	15.392600151304562	43.48378754024525	21.432027305195376	19.691585003254808
23	16.138566829113813	44.229094459789934	17.47897570330231	22.153363007793946
24	14.340065799890919	45.300541881454635	23.47309945635919	16.886292862295253
25	12.602482450430163	44.96384524710146	21.879343408575096	20.554328893893278
26	15.087351994229314	49.21972589243301	18.75164939566143	16.94127271767624
27	12.25522968384384	46.877364133781384	22.267281268143353	18.600124914231426
28	13.669971322507433	46.56265944158061	23.580200214641355	16.187169021270606
29	13.282913140625274	44.787689790460774	23.37347595840884	18.55592111050511
30	15.067339326870636	45.25655799714984	22.522387797111136	17.15371487886838
31	18.902734038248386	45.35948028642306	17.603670015306392	18.134115660022168
32	20.26403525748166	43.63685145762592	17.93398898643537	18.165124298457044
33	18.51919456711061	44.56315206108482	17.74089973433734	19.176753637467232
34	16.37585988493816	44.13870757754359	19.295730044511693	20.18970249300656
35	16.792827108147577	43.42308977990464	20.896743433206073	18.88733967874171
36	20.284927602526434	38.910123330811594	23.122108059606962	17.682841007055014
37	18.946058164288605	40.03589084959271	22.463449392142717	18.554601593975967
38	20.278989778145288	37.38234310948469	21.152289801016906	21.18637731135312
39	20.18970249300656	36.673322894491456	23.872033287003642	19.264941325498338
40	20.955901757596017	36.301439152694456	22.673472439698095	20.069186650011435
41	18.04130966413906	33.49790636710709	26.690080754411582	21.770703214342266
42	23.671906613416844	34.89043614419677	21.752010063512728	19.685647178873662
43	20.087439961997923	35.15653864424075	24.104048276711413	20.651973117049913
44	18.184037368708104	36.818909551540315	23.841464487411812	21.155588592339765
45	18.83345942046834	39.52105948380513	21.795774028395996	19.84970706733053
46	18.709644786150353	36.10417143158747	27.57217755414416	17.61400622811802
47	19.45693098048875	35.5077499604145	23.77680817748377	21.258510881612978
48	20.34320624923028	32.25272260243847	27.53545101074966	19.86862013758159
49	20.08216189588135	31.11705870968877	26.53261844860043	22.26816094582945
50	17.001310719752283	34.450377381727336	24.71520434912648	23.833107549393905
51	17.07058533753233	35.431437921145694	25.919922940234695	21.578053801087282
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24037.0
1	19172.0
2	14307.0
3	9797.0
4	5287.0
5	5008.5
6	4730.0
7	5156.5
8	5583.0
9	6089.5
10	6596.0
11	7255.5
12	7915.0
13	8095.5
14	8276.0
15	7945.5
16	7615.0
17	7275.0
18	6935.0
19	6640.0
20	6345.0
21	5672.5
22	5000.0
23	4648.5
24	4297.0
25	4422.5
26	5176.0
27	5804.0
28	5871.0
29	5938.0
30	6246.0
31	6554.0
32	7906.5
33	9259.0
34	9229.5
35	9200.0
36	9917.5
37	10635.0
38	11672.0
39	12709.0
40	15007.0
41	17305.0
42	21138.5
43	24972.0
44	32023.5
45	39075.0
46	39323.0
47	39571.0
48	38403.5
49	37236.0
50	33699.0
51	30162.0
52	25604.0
53	21046.0
54	18890.0
55	16734.0
56	14674.5
57	12615.0
58	11012.0
59	9409.0
60	8800.0
61	8191.0
62	7921.0
63	7651.0
64	6482.0
65	5313.0
66	5041.5
67	4770.0
68	3718.5
69	2667.0
70	2421.0
71	2175.0
72	2008.5
73	1842.0
74	1367.0
75	822.5
76	753.0
77	533.0
78	313.0
79	208.5
80	104.0
81	84.5
82	65.0
83	97.5
84	130.0
85	106.0
86	82.0
87	59.0
88	36.0
89	25.5
90	15.0
91	12.0
92	9.0
93	6.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	454712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.17641557955096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.01296076412166	30.861243278559453
2	7.435495012297821	5.528501052337244
3	2.6512788050950964	2.9569512802641182
4	1.3792807875028026	2.0510686302837806
5	0.8661109187441371	1.6099449726609367
6	0.6060433215881742	1.3518311029544048
7	0.45270973599279735	1.1781087697523924
8	0.36916889905980915	1.0979501128394213
9	0.27854250047830015	0.9319690578913704
>10	2.2535683052048916	17.854829715630103
>50	0.45741692291083275	12.113858713551902
>100	0.23141329214279974	12.730378168841227
>500	0.0012021469721703883	0.27462936228727175
>1k	0.004207514402596359	4.865565284331208
>5k	0.0	0.0
>10k+	6.010734860851942E-4	4.593170497815191
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20555	4.520443709424867	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4927	1.0835429898485194	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4161	0.915084712961171	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	3021	0.6643765724238639	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGT	2834	0.6232516405988845	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2461	0.5412216963704499	No Hit
AATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT	2201	0.48404264677422193	No Hit
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	2169	0.47700522528545547	TruSeq Adapter, Index 16 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT	620	0.13635004134485126	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	609	0.13393092770808776	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCT	488	0.10732067770368937	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3983884304790727E-4	0.0	0.0	0.687688031105403	0.0
2	4.3983884304790727E-4	0.0	0.0	0.825137669557874	0.0
3	4.3983884304790727E-4	0.0	0.0	1.0877214588574746	0.0
4	4.3983884304790727E-4	0.0	0.0	2.599227642991608	0.0
5	4.3983884304790727E-4	0.0	0.0	2.7907774591389716	0.0
6	4.3983884304790727E-4	0.0	0.0	3.8184609159204066	0.0
7	4.3983884304790727E-4	0.0	0.0	4.678125934657541	0.0
8	4.3983884304790727E-4	0.0	0.0	5.297419025668995	0.0
9	4.3983884304790727E-4	0.0	0.0	6.864784742870213	0.0
10	4.3983884304790727E-4	0.0	0.0	8.099632294727211	0.0
11	4.3983884304790727E-4	0.0	0.0	10.05075740248773	0.0
12	4.3983884304790727E-4	0.0	0.0	10.756038987315048	0.0
13	4.3983884304790727E-4	0.0	0.0	11.098893365470891	0.0
14	4.3983884304790727E-4	0.0	0.0	11.470117349003326	0.0
15	4.3983884304790727E-4	0.0	0.0	11.70235225813262	0.0
16	4.3983884304790727E-4	0.0	0.0	12.066978659019336	0.0
17	4.3983884304790727E-4	0.0	0.0	12.58510881612977	0.0
18	4.3983884304790727E-4	0.0	0.0	13.231891834831718	0.0
19	4.3983884304790727E-4	0.0	0.0	13.508770386530376	0.0
20	4.3983884304790727E-4	0.0	0.0	13.821275884515913	0.0
21	4.3983884304790727E-4	0.0	0.0	14.108270729604673	0.0
22	4.3983884304790727E-4	0.0	0.0	14.421435985854783	0.0
23	4.3983884304790727E-4	0.0	0.0	14.729763014831367	0.0
24	4.3983884304790727E-4	0.0	0.0	14.92527138056616	0.0
25	4.3983884304790727E-4	0.0	0.0	15.092190221502841	0.0
26	4.3983884304790727E-4	0.0	0.0	15.28066116574887	0.0
27	4.3983884304790727E-4	0.0	0.0	15.48760534140291	0.0
28	4.3983884304790727E-4	0.0	0.0	15.661561603828357	0.0
29	4.3983884304790727E-4	0.0	0.0	15.868285860060874	0.0
30	4.3983884304790727E-4	0.0	0.0	16.138346909692288	0.0
31	4.3983884304790727E-4	0.0	0.0	16.352548426256618	0.0
32	4.3983884304790727E-4	0.0	0.0	16.52936364116188	0.0
33	4.3983884304790727E-4	0.0	0.0	16.712776438712854	0.0
34	4.3983884304790727E-4	0.0	0.0	16.9032266577526	0.0
35	4.3983884304790727E-4	0.0	0.0	17.12578511233484	0.0
36	4.3983884304790727E-4	0.0	0.0	17.317994686746776	0.0
37	4.3983884304790727E-4	0.0	0.0	17.521420151656432	0.0
38	4.3983884304790727E-4	0.0	0.0	17.712750048382272	0.0
39	4.3983884304790727E-4	0.0	0.0	17.90276042857897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGC	20	7.028783E-4	45.000004	19
TCACGCG	20	7.028783E-4	45.000004	1
TATTTCG	20	7.028783E-4	45.000004	27
AGACTCG	20	7.028783E-4	45.000004	1
GTACCAC	20	7.028783E-4	45.000004	23
CGTATAA	20	7.028783E-4	45.000004	20
GGTCGGT	20	7.028783E-4	45.000004	12
CAAGTAC	20	7.028783E-4	45.000004	24
CTCCGAG	20	7.028783E-4	45.000004	30
GGTCGAG	20	7.028783E-4	45.000004	18
CACGGCC	20	7.028783E-4	45.000004	20
AGATACG	20	7.028783E-4	45.000004	1
CGAAACG	20	7.028783E-4	45.000004	14
TCCGATC	20	7.028783E-4	45.000004	14
TAGACGA	20	7.028783E-4	45.000004	12
GCGCGAC	20	7.028783E-4	45.000004	9
CCGGCTA	20	7.028783E-4	45.000004	30
ACCGCAA	20	7.028783E-4	45.000004	29
GACAATC	20	7.028783E-4	45.000004	45
CGGTTCG	20	7.028783E-4	45.000004	15
>>END_MODULE