FastQCFastQC Report
Sat 18 Jun 2016
SRR3548372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571733
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132742.3217131073420636No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33080.5785917552423946No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27700.48449188694722883No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT25040.43796667325482347No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18070.31605662083525005No Hit
AAAGCCGGGACCCAAGATTATTGCATAATCTTCACCAGCTTGCTTTGATTC16850.29471798899136487No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT11510.2013177479697691No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC10940.19134805932139654No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT9440.16511203656252132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA302.1635806E-645.00000422
TGTTTCG302.1635806E-645.00000444
GTTTCGA302.1635806E-645.00000445
CCGATTG302.1635806E-645.00000433
ACTTCGC302.1635806E-645.00000424
CGATTGT302.1635806E-645.00000434
CTTGGCG302.1635806E-645.0000041
CTAACCG302.1635806E-645.0000041
TCGGACG302.1635806E-645.00000410
ACGGCCG302.1635806E-645.00000429
GTCCGTT302.1635806E-645.00000410
TACTCGC1250.045.00000445
ACGTCCA600.045.00000419
TTACGTC600.045.00000417
TTAAACG302.1635806E-645.0000041
TTACGCA302.1635806E-645.00000425
CAGTCGA600.045.00000434
CGTCCAA600.045.00000420
CTTAGAC302.1635806E-645.00000444
AGTCGAG600.045.00000435