##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548372_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571733
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.116239573367288	31.0	31.0	34.0	28.0	34.0
2	31.290835057623053	31.0	31.0	34.0	28.0	34.0
3	31.49508074573271	31.0	31.0	34.0	30.0	34.0
4	35.43912980359713	37.0	35.0	37.0	33.0	37.0
5	35.026930402827894	37.0	35.0	37.0	32.0	37.0
6	35.02001633629684	36.0	35.0	37.0	32.0	37.0
7	34.270117344984456	36.0	35.0	37.0	31.0	37.0
8	34.622556333113536	37.0	35.0	37.0	32.0	37.0
9	35.77263862677159	39.0	35.0	39.0	31.0	39.0
10	35.40730200985425	37.0	35.0	39.0	30.0	39.0
11	35.81885775353181	37.0	35.0	39.0	30.0	39.0
12	35.56049239767514	37.0	35.0	39.0	30.0	39.0
13	35.12397220380842	37.0	34.0	39.0	29.0	39.0
14	36.19499486648488	38.0	35.0	40.0	30.0	41.0
15	36.415473656409546	38.0	35.0	40.0	31.0	41.0
16	36.439122807324395	38.0	35.0	40.0	31.0	41.0
17	36.42472447803433	38.0	35.0	40.0	31.0	41.0
18	36.22575048143102	38.0	35.0	40.0	31.0	41.0
19	36.18739516522572	38.0	34.0	40.0	31.0	41.0
20	36.05391502676949	38.0	34.0	40.0	30.0	41.0
21	36.043668985348056	38.0	34.0	40.0	30.0	41.0
22	36.025009925961946	38.0	34.0	40.0	30.0	41.0
23	35.92794363802684	38.0	34.0	40.0	30.0	41.0
24	35.56466217622562	37.0	34.0	40.0	29.0	41.0
25	35.64109295772677	37.0	34.0	40.0	30.0	41.0
26	35.51292299027693	37.0	34.0	40.0	29.0	41.0
27	35.356907857339	37.0	34.0	40.0	29.0	41.0
28	35.331235034535354	37.0	34.0	40.0	29.0	41.0
29	35.229442764367285	37.0	34.0	40.0	27.0	41.0
30	35.1934259523239	37.0	34.0	40.0	27.0	41.0
31	34.97106341596515	37.0	34.0	40.0	27.0	41.0
32	34.93042206764346	37.0	34.0	40.0	27.0	41.0
33	34.79196583020396	37.0	33.0	40.0	26.0	41.0
34	34.75645100072936	37.0	34.0	40.0	26.0	41.0
35	34.65498230817532	37.0	33.0	40.0	25.0	41.0
36	34.40388083248649	36.0	33.0	40.0	24.0	41.0
37	34.251293873189056	36.0	33.0	40.0	24.0	41.0
38	34.16122560705784	36.0	33.0	40.0	24.0	41.0
39	34.0951300694555	36.0	33.0	40.0	23.0	41.0
40	33.863988959881624	36.0	33.0	40.0	23.0	41.0
41	33.90984428045959	36.0	33.0	40.0	23.0	41.0
42	33.82309399667327	36.0	33.0	40.0	23.0	41.0
43	33.76912125065371	35.0	33.0	40.0	23.0	41.0
44	33.667113845099024	35.0	33.0	39.0	23.0	41.0
45	33.49608995807483	35.0	33.0	39.0	23.0	40.0
46	33.37371990072289	35.0	32.0	39.0	22.0	40.0
47	33.284263808456046	35.0	32.0	39.0	22.0	40.0
48	33.19678591230522	35.0	32.0	39.0	21.0	40.0
49	33.05853956304779	35.0	32.0	39.0	20.0	40.0
50	32.8329272580033	35.0	32.0	39.0	20.0	40.0
51	30.95352550928493	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	7.0
11	7.0
12	7.0
13	15.0
14	50.0
15	98.0
16	183.0
17	342.0
18	720.0
19	1289.0
20	2140.0
21	3129.0
22	4296.0
23	5298.0
24	6395.0
25	7105.0
26	8003.0
27	9577.0
28	11601.0
29	14126.0
30	17928.0
31	22681.0
32	29919.0
33	41196.0
34	64983.0
35	65095.0
36	57056.0
37	72497.0
38	82641.0
39	43347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.755200416977857	34.25445094126104	21.44637444401495	24.543974197746152
2	21.453545623569042	35.15889409916867	21.093587391317275	22.293972885945013
3	18.937685947811303	37.3088487108493	19.38492268244093	24.36854265889847
4	18.672877024765057	36.962358303613755	19.431972616588514	24.93279205503268
5	17.164305716129732	40.0750000437267	16.613524145011745	26.147170095131816
6	17.20890695481982	42.41787687609426	18.339854442545732	22.033361726540186
7	79.0211514815482	14.123725585194489	3.320431040363246	3.534691892894061
8	82.62405703361534	11.704064659552623	2.580050478107788	3.0918278287242473
9	76.08971320528988	16.267733365049768	4.689251801102962	2.9533016285573863
10	39.04462397657648	42.146946214404274	8.813554578798145	9.9948752302211
11	32.74413056444179	29.978503952019565	23.25316187800949	14.024203605529154
12	32.91956210328947	28.53587251391821	22.885332838930058	15.65923254386226
13	21.510040525909822	41.96714200509678	19.827961653429135	16.694855815564257
14	15.305221143435835	43.73072745494838	24.143612490445715	16.820438911170076
15	14.090143476063128	34.32231478679733	33.99803754549763	17.589504191641904
16	16.183778092221367	32.084731859102064	33.186120094519644	18.545369954156925
17	16.704650597394238	30.929647230437986	25.43757313291344	26.92812903925434
18	20.28954074716695	32.75759838945802	26.397811565888276	20.555049297486764
19	23.1760279710984	34.42183676646267	23.3392160326586	19.062919229780334
20	25.134109803002453	33.71416377924661	22.327380088257982	18.824346329492965
21	19.51138031213871	36.93927060358594	24.129095224519137	19.420253859756215
22	20.177600383395745	33.811761783909624	22.329653876897083	23.68098395579755
23	18.76137987487166	36.78010539884876	21.260098682426936	23.19841604385264
24	15.765750796263292	35.39764190627443	29.638660003882933	19.197947293579347
25	16.77513804520642	34.89286082839367	27.08572008262598	21.24628104377393
26	18.346675808463043	40.04299209596088	22.23852742451459	19.371804671061494
27	16.51452688580159	38.45816141450642	25.09003328476754	19.93727841492445
28	14.89663881567095	38.63166897835178	28.61982778674661	17.851864419230655
29	17.916579942035877	33.701570488322346	27.888017658592386	20.493831911049387
30	18.989458366055484	36.558673366763855	24.50531979088141	19.946548476299252
31	23.760391651347746	37.070275810561924	20.349358879057185	18.819973659033153
32	25.4478926351986	33.27794617417571	21.679350326113763	19.59481086451193
33	21.812279508092065	34.90213088976847	21.080644286756232	22.204945315383227
34	18.988234018326736	35.93810397510726	23.048870714127048	22.02479129243895
35	20.165706719745057	33.61656577458359	24.637549345586137	21.580178160085215
36	24.46858935901898	34.01342934551618	22.948649107188146	18.569332188276697
37	19.69328340326691	36.73830266925295	23.572891542030984	19.995522385449153
38	19.539365403081508	36.88662365124979	23.12145704375994	20.452553901908757
39	19.954769096763698	35.53756736098843	22.726517447829668	21.7811460944182
40	22.507883924839042	33.16548108994933	22.86784215709081	21.458792828120817
41	18.91162483187082	30.212704181847123	25.294674262286765	25.58099672399529
42	23.279222993949976	30.669385884669943	22.29729611549447	23.754095005885613
43	21.424161278079104	30.966027848663625	23.88195189013053	23.72785898312674
44	20.19596559932696	34.75398481459003	23.655447560312243	21.39460202577077
45	19.91558996944378	37.73072395681201	22.21893786085463	20.134748212889583
46	20.222376528904228	35.62537058382147	25.519779337557917	18.632473549716387
47	20.922703429747802	32.70005404620689	23.68798022853325	22.689262295512066
48	22.256542826809017	30.31047709332853	26.93425077789807	20.498729301964378
49	21.81752671264384	29.449410826382245	27.023103441641467	21.70995901933245
50	19.187277977657402	34.467312539244716	23.99564132208566	22.34976816101222
51	19.643434960025047	33.01960880340998	24.302252974727715	23.034703261837254
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15656.0
1	12384.0
2	9112.0
3	6015.0
4	2918.0
5	2493.5
6	2069.0
7	2252.5
8	2436.0
9	2649.0
10	2862.0
11	3085.5
12	3309.0
13	3464.5
14	3620.0
15	3713.5
16	3807.0
17	3762.5
18	3718.0
19	3290.5
20	2863.0
21	3149.0
22	3435.0
23	3241.0
24	3047.0
25	3521.5
26	4411.0
27	4826.0
28	6796.0
29	8766.0
30	8604.0
31	8442.0
32	10386.5
33	12331.0
34	13895.0
35	15459.0
36	16824.0
37	18189.0
38	18667.5
39	19146.0
40	24696.5
41	30247.0
42	36044.0
43	41841.0
44	47182.5
45	52524.0
46	52854.0
47	53184.0
48	50915.0
49	48646.0
50	46635.0
51	44624.0
52	40841.5
53	37059.0
54	32827.5
55	28596.0
56	24801.5
57	21007.0
58	18162.5
59	15318.0
60	14250.5
61	13183.0
62	11800.0
63	10417.0
64	9100.0
65	7783.0
66	6884.0
67	5985.0
68	4870.5
69	3756.0
70	3191.5
71	2627.0
72	2093.0
73	1559.0
74	1397.0
75	1042.0
76	849.0
77	724.0
78	599.0
79	472.5
80	346.0
81	243.0
82	140.0
83	118.0
84	96.0
85	81.5
86	67.0
87	46.0
88	25.0
89	16.0
90	7.0
91	5.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	571733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.6834888061883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.04800011085237	22.960559151373936
2	8.737704633506823	5.012557060939452
3	3.1610504737581353	2.720098676395131
4	1.5904435752854538	1.8247788195429764
5	0.9536753123329088	1.367736757301956
6	0.6152956569150664	1.0589295652571753
7	0.4323081481615647	0.8680074150031364
8	0.31254541632319666	0.7171914360425498
9	0.2525551659777657	0.6519746948641445
>10	2.5829390631296025	16.861660867666444
>50	0.7322680164438622	15.164425463148998
>100	0.5756198499380446	25.703869655634943
>500	6.216197083564195E-4	0.16831729517351943
>1k	0.004351337958494937	2.5531094804974837
>5k	0.0	0.0
>10k+	6.216197083564195E-4	2.3667836611581534
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13274	2.3217131073420636	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3308	0.5785917552423946	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2770	0.48449188694722883	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	2504	0.43796667325482347	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1807	0.31605662083525005	No Hit
AAAGCCGGGACCCAAGATTATTGCATAATCTTCACCAGCTTGCTTTGATTC	1685	0.29471798899136487	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT	1151	0.2013177479697691	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	1094	0.19134805932139654	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	944	0.16511203656252132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.62441734166123	0.0
2	0.0	0.0	0.0	0.754548014545251	0.0
3	0.0	0.0	0.0	0.9245574420227624	0.0
4	0.0	0.0	0.0	1.8246279294705745	0.0
5	0.0	0.0	0.0	1.9425151250671204	0.0
6	0.0	0.0	0.0	2.5968415326734684	0.0
7	0.0	0.0	0.0	3.153744842435193	0.0
8	0.0	0.0	0.0	3.6196966066328162	0.0
9	0.0	0.0	0.0	4.459424241735215	0.0
10	0.0	0.0	0.0	5.501693972536131	0.0
11	0.0	0.0	0.0	6.6057058102296	0.0
12	0.0	0.0	0.0	7.148441667701532	0.0
13	0.0	0.0	0.0	7.355531340678254	0.0
14	0.0	0.0	0.0	7.56786821820675	0.0
15	0.0	0.0	0.0	7.738052552502654	0.0
16	0.0	0.0	0.0	8.074923084726612	0.0
17	0.0	0.0	0.0	8.533703669370142	0.0
18	0.0	0.0	0.0	9.096903624593997	0.0
19	0.0	0.0	0.0	9.334601990789407	0.0
20	0.0	0.0	0.0	9.597836752470121	0.0
21	0.0	0.0	0.0	9.893779089190234	0.0
22	0.0	0.0	0.0	10.213858566848511	0.0
23	0.0	0.0	0.0	10.53183916268608	0.0
24	0.0	0.0	0.0	10.765164858421675	0.0
25	0.0	0.0	0.0	10.964908445025912	0.0
26	0.0	0.0	0.0	11.168150167997998	0.0
27	0.0	0.0	0.0	11.378038350069001	0.0
28	0.0	0.0	0.0	11.589325786687143	0.0
29	0.0	0.0	0.0	11.83244626425272	0.0
30	0.0	0.0	0.0	12.109848478223226	0.0
31	0.0	0.0	0.0	12.359790321706111	0.0
32	0.0	0.0	0.0	12.5792983787887	0.0
33	0.0	0.0	0.0	12.776068549480264	0.0
34	0.0	0.0	0.0	13.007645177031936	0.0
35	0.0	0.0	0.0	13.257412113696429	0.0
36	0.0	0.0	0.0	13.497734082167725	0.0
37	0.0	0.0	0.0	13.72948561653779	0.0
38	0.0	0.0	0.0	13.951617275896266	0.0
39	0.0	0.0	0.0	14.185292785268649	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	30	2.1635806E-6	45.000004	22
TGTTTCG	30	2.1635806E-6	45.000004	44
GTTTCGA	30	2.1635806E-6	45.000004	45
CCGATTG	30	2.1635806E-6	45.000004	33
ACTTCGC	30	2.1635806E-6	45.000004	24
CGATTGT	30	2.1635806E-6	45.000004	34
CTTGGCG	30	2.1635806E-6	45.000004	1
CTAACCG	30	2.1635806E-6	45.000004	1
TCGGACG	30	2.1635806E-6	45.000004	10
ACGGCCG	30	2.1635806E-6	45.000004	29
GTCCGTT	30	2.1635806E-6	45.000004	10
TACTCGC	125	0.0	45.000004	45
ACGTCCA	60	0.0	45.000004	19
TTACGTC	60	0.0	45.000004	17
TTAAACG	30	2.1635806E-6	45.000004	1
TTACGCA	30	2.1635806E-6	45.000004	25
CAGTCGA	60	0.0	45.000004	34
CGTCCAA	60	0.0	45.000004	20
CTTAGAC	30	2.1635806E-6	45.000004	44
AGTCGAG	60	0.0	45.000004	35
>>END_MODULE