##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548371_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615846
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02454672109586	31.0	31.0	34.0	28.0	34.0
2	31.340612750590246	31.0	31.0	34.0	28.0	34.0
3	31.49945765662195	31.0	31.0	34.0	30.0	34.0
4	35.48291943115649	37.0	35.0	37.0	33.0	37.0
5	35.047409579667644	37.0	35.0	37.0	32.0	37.0
6	35.20013769676185	37.0	35.0	37.0	32.0	37.0
7	35.14235214647818	37.0	35.0	37.0	32.0	37.0
8	35.55465976883831	37.0	35.0	37.0	35.0	37.0
9	36.92589543489768	39.0	37.0	39.0	33.0	39.0
10	36.16224348294866	37.0	35.0	39.0	31.0	39.0
11	36.403367075535115	38.0	35.0	39.0	32.0	39.0
12	36.02180252855422	37.0	35.0	39.0	32.0	39.0
13	35.489474641387616	37.0	35.0	39.0	30.0	39.0
14	36.65110271074262	38.0	35.0	40.0	31.0	41.0
15	36.71702341169708	38.0	35.0	40.0	31.0	41.0
16	36.66595869746657	38.0	35.0	40.0	32.0	41.0
17	36.68049155145929	38.0	35.0	40.0	32.0	41.0
18	36.4939936282772	38.0	35.0	40.0	31.0	41.0
19	36.68877121877872	38.0	35.0	40.0	32.0	41.0
20	36.399312490460275	38.0	35.0	40.0	31.0	41.0
21	36.472752928491865	38.0	35.0	40.0	31.0	41.0
22	36.56701675418855	38.0	35.0	40.0	31.0	41.0
23	36.39195513164005	38.0	35.0	40.0	31.0	41.0
24	35.999891206567874	38.0	34.0	40.0	30.0	41.0
25	36.197818935253295	38.0	35.0	40.0	31.0	41.0
26	36.21007686986682	38.0	35.0	40.0	31.0	41.0
27	36.09623672151804	38.0	34.0	40.0	31.0	41.0
28	36.11960782403393	38.0	35.0	40.0	31.0	41.0
29	35.92678526774551	38.0	35.0	40.0	30.0	41.0
30	35.87899897052185	38.0	35.0	40.0	30.0	41.0
31	35.75456364091023	38.0	34.0	40.0	30.0	41.0
32	35.7753464340111	38.0	34.0	40.0	30.0	41.0
33	35.64764243008804	38.0	34.0	40.0	30.0	41.0
34	35.64420325860686	38.0	35.0	40.0	30.0	41.0
35	35.4582119555863	38.0	34.0	40.0	29.0	41.0
36	35.3232983570568	37.0	34.0	40.0	29.0	41.0
37	35.182943463138514	37.0	34.0	40.0	28.0	41.0
38	35.01394991605044	37.0	34.0	40.0	27.0	41.0
39	34.99758543531987	37.0	34.0	40.0	27.0	41.0
40	34.796218535153265	37.0	34.0	40.0	26.0	41.0
41	34.88709352662841	37.0	34.0	40.0	26.0	41.0
42	34.788732897510094	37.0	34.0	40.0	26.0	41.0
43	34.73354052798914	37.0	34.0	40.0	26.0	41.0
44	34.63955924045946	37.0	34.0	40.0	26.0	41.0
45	34.43375454253174	36.0	33.0	40.0	25.0	41.0
46	33.48429315120988	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	11.0
11	5.0
12	7.0
13	10.0
14	20.0
15	40.0
16	99.0
17	154.0
18	270.0
19	513.0
20	942.0
21	1632.0
22	2576.0
23	3637.0
24	4674.0
25	5626.0
26	6624.0
27	7574.0
28	8818.0
29	11066.0
30	14386.0
31	19555.0
32	26382.0
33	38273.0
34	66493.0
35	75308.0
36	60363.0
37	81058.0
38	108596.0
39	71131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.535240953095418	33.493438294638594	21.630732358414278	25.34058839385171
2	21.986503119286315	34.17104276069017	20.435628387616383	23.40682573240713
3	19.082692751109857	37.162212631079846	19.30417669352403	24.450917924286266
4	18.361895668722376	36.75967693222007	19.64273535916447	25.23569203989309
5	17.349629615196008	39.41553570210735	16.784066146406733	26.450768536289914
6	17.537338880174588	41.79064246581126	18.66749154821173	22.004527105802424
7	80.27299032550346	12.192171419478246	3.7994888332472727	3.7353494217710272
8	82.9161835913524	10.841833835082147	2.9344998587309163	3.307482714834553
9	76.96810566277932	14.406198952335487	5.452499488508491	3.1731958963766917
10	38.54941007979267	41.863225546646404	9.03667475310386	10.550689620457062
11	31.790902271022304	29.652380627624435	24.06153486423554	14.49518223711772
12	32.061262068763945	28.414895931775153	23.542411576920205	15.981430422540699
13	21.624237228138206	41.0373697320434	20.37165135439704	16.966741685421354
14	15.162881629498285	42.92241891641741	24.769828820841575	17.144870633242725
15	14.082254329816221	34.03334599883737	33.77159224871153	18.11280742263488
16	16.030955790895778	32.22510172997795	32.886955505110045	18.85698697401623
17	16.397443516723335	31.002555833763633	25.89202495429052	26.70797569522251
18	20.007274545909205	32.333732783845306	26.642862014204848	21.016130656040634
19	22.854414902426907	34.25807750638958	23.90857454623396	18.97893304494955
20	24.54428542200485	33.55546678877512	22.99747014675747	18.902777642462564
21	19.284528924438902	36.96443591417335	24.648694641192765	19.102340520194982
22	19.769715156061743	33.731971954027465	22.76721777847059	23.731095111440197
23	18.883129873377435	36.60541758816328	21.317017566079834	23.194434972379458
24	15.430480996872594	35.42573955177106	29.446160241359042	19.697619209997306
25	16.49048625792812	34.96393578914209	27.311535676126823	21.234042276802967
26	18.506899452135762	39.50468136514648	22.64575884230798	19.34266034040978
27	16.427321115993283	37.89502570447807	25.157100963552576	20.52055221597607
28	15.043533610675397	38.53333463235939	28.5998447663864	17.82328699057881
29	18.043634285194678	33.66799492080813	27.519542223218142	20.768828570779057
30	18.682267969589798	36.350970859598014	24.66964143633311	20.297119734479075
31	23.265394270645583	37.34245249624094	20.41711726632957	18.975035966783903
32	25.252904135124695	32.986818133104705	21.73985054705235	20.020427184718255
33	21.37725989939043	34.851894791879786	21.349493217460207	22.42135209126957
34	18.79236042776928	35.597698125830156	23.258574383855706	22.35136706254486
35	20.223237627588716	33.49814726408875	24.707800326705055	21.570814781617482
36	23.829333956865838	34.04958382452756	23.284230148446202	18.836852070160397
37	19.417029582070843	36.80043387470244	23.37158965065942	20.410946892567296
38	19.575673139063987	36.72720128083969	23.204502424307375	20.492623155788948
39	19.751691169545634	35.44782299470972	22.738963961769663	22.06152187397499
40	22.376860448878453	33.29744773855802	22.719316192684534	21.606375619878996
41	18.8626702130078	30.382270892398424	25.261347804483584	25.49371109011019
42	23.480383082783682	30.657826794360926	22.475099294304098	23.386690828551295
43	21.205301325331334	30.927049944304258	24.23268154701013	23.63496718335428
44	20.20829882795374	34.24346346326841	23.48022070452678	22.06801700425106
45	19.802677942212828	37.251195915862084	22.80976088177889	20.136365260146206
46	19.879969992498125	35.368257648827786	26.087365997343493	18.66440636133059
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19415.0
1	15424.5
2	11434.0
3	7589.0
4	3744.0
5	3268.5
6	2793.0
7	2793.0
8	2847.5
9	2902.0
10	3000.5
11	3099.0
12	3338.0
13	3577.0
14	3501.0
15	3425.0
16	3556.5
17	3688.0
18	3744.0
19	3800.0
20	3800.0
21	3541.5
22	3283.0
23	3674.5
24	4066.0
25	4167.0
26	4268.0
27	6077.5
28	7887.0
29	8029.5
30	8172.0
31	8172.0
32	9663.5
33	11155.0
34	12595.0
35	14035.0
36	16543.0
37	19051.0
38	23379.5
39	27708.0
40	29898.5
41	32089.0
42	40732.5
43	49376.0
44	49376.0
45	55488.5
46	61601.0
47	66442.0
48	71283.0
49	65048.5
50	58814.0
51	52046.0
52	45278.0
53	41649.5
54	38021.0
55	32385.0
56	26749.0
57	26749.0
58	23712.5
59	20676.0
60	18111.0
61	15546.0
62	13620.5
63	11695.0
64	10595.5
65	9496.0
66	8569.5
67	7643.0
68	5750.5
69	3858.0
70	3858.0
71	2743.5
72	1629.0
73	1752.5
74	1876.0
75	1264.5
76	653.0
77	755.0
78	857.0
79	803.0
80	749.0
81	749.0
82	557.0
83	365.0
84	222.5
85	80.0
86	44.0
87	8.0
88	5.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	615846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.333980277848866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.86009049847856	17.701178555673287
2	10.021266246927235	4.676720579297452
3	3.824863064995232	2.677478379722139
4	1.92940833291972	1.8008310395306402
5	1.1477356213056031	1.3390620175864778
6	0.8347235388948776	1.1686453556417569
7	0.5876975002513707	0.9599325316144592
8	0.4551685590725628	0.8496715344396853
9	0.3313320303509717	0.6958165555466275
>10	3.3058682619872846	17.360791425605484
>50	0.9057876233602831	15.232983843857905
>100	0.7883707092209872	29.525953996132863
>500	0.0034945510160504746	0.6309703283716918
>1k	0.0034945510160504746	2.715830674172598
>5k	0.0	0.0
>10k+	6.98910203210095E-4	2.6641331828069155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16336	2.652611204749239	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTT	4663	0.7571698119335029	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4205	0.6828005702724382	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3420	0.5553336386044563	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2210	0.3588559477531721	No Hit
AAAGCCGGGACCCAAGATTATTGCATAATCTTCACCAGCTTGCTTT	2155	0.34992514362356825	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTAT	989	0.16059209607596706	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGT	899	0.14597805295479713	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT	882	0.14321762258746504	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8627156789197299	0.0
2	0.0	0.0	0.0	1.0414941397687083	0.0
3	0.0	0.0	0.0	1.2696355907158607	0.0
4	0.0	0.0	0.0	2.3384092776440863	0.0
5	0.0	0.0	0.0	2.483575439314374	0.0
6	0.0	0.0	0.0	3.3078074713483567	0.0
7	0.0	0.0	0.0	3.9453045079451683	0.0
8	0.0	0.0	0.0	4.515901702698402	0.0
9	0.0	0.0	0.0	5.452012353737785	0.0
10	0.0	0.0	0.0	6.7271688051883105	0.0
11	0.0	0.0	0.0	8.01823832581522	0.0
12	0.0	0.0	0.0	8.644044127915096	0.0
13	0.0	0.0	0.0	8.904011717215019	0.0
14	0.0	0.0	0.0	9.138323541924443	0.0
15	0.0	0.0	0.0	9.339672580482784	0.0
16	0.0	0.0	0.0	9.756010431179224	0.0
17	0.0	0.0	0.0	10.30192613088337	0.0
18	0.0	0.0	0.0	10.97027503629154	0.0
19	0.0	0.0	0.0	11.263367789999448	0.0
20	0.0	0.0	0.0	11.571886478113035	0.0
21	0.0	0.0	0.0	11.9214868652228	0.0
22	1.623782569018878E-4	0.0	0.0	12.289435995362476	0.0
23	1.623782569018878E-4	0.0	0.0	12.672323925137128	0.0
24	1.623782569018878E-4	0.0	0.0	12.948366961870338	0.0
25	1.623782569018878E-4	0.0	0.0	13.176995547588195	0.0
26	1.623782569018878E-4	0.0	0.0	13.410008346242405	0.0
27	1.623782569018878E-4	0.0	0.0	13.643833036181123	0.0
28	1.623782569018878E-4	0.0	0.0	13.87830723914745	0.0
29	1.623782569018878E-4	0.0	0.0	14.142821419640624	0.0
30	1.623782569018878E-4	0.0	0.0	14.487712837300235	0.0
31	1.623782569018878E-4	0.0	0.0	14.779181808439123	0.0
32	1.623782569018878E-4	0.0	0.0	15.039311775995946	0.0
33	1.623782569018878E-4	0.0	0.0	15.265179931346474	0.0
34	1.623782569018878E-4	0.0	0.0	15.52628416844471	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	25	7.790432E-5	40.000004	2
CTTCGTT	25	7.790432E-5	40.000004	23
TCGCCAA	25	7.790432E-5	40.000004	29
GTGTTCG	25	7.790432E-5	40.000004	9
TTGATCG	25	7.790432E-5	40.000004	1
AGCCGTC	25	7.790432E-5	40.000004	37
CGGAACG	25	7.790432E-5	40.000004	1
GTCGCCA	25	7.790432E-5	40.000004	28
GTCGACT	25	7.790432E-5	40.000004	34
CGAGTCT	25	7.790432E-5	40.000004	18
GCGCGAC	50	7.8216544E-11	40.000004	9
CCTACGC	25	7.790432E-5	40.000004	12
TTCGTTG	25	7.790432E-5	40.000004	24
TTCGTCG	25	7.790432E-5	40.000004	31
ATCGTCG	25	7.790432E-5	40.000004	14
GTTACGC	25	7.790432E-5	40.000004	24
GTTACCG	25	7.790432E-5	40.000004	1
TCAATCG	25	7.790432E-5	40.000004	1
CCACTAC	25	7.790432E-5	40.000004	30
TCCAGCG	25	7.790432E-5	40.000004	1
>>END_MODULE