FastQCFastQC Report
Sat 18 Jun 2016
SRR3548370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559380
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204033.647431084414888No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGT62441.1162358325288713No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC60851.0878115055954807No Hit
AATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT57221.0229182308984948No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49250.8804390575279774No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37170.6644856805749223No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT24670.44102399084700916Illumina Single End Adapter 2 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19890.3555722406950553No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTT15650.27977403553934715No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT12490.22328292037613073No Hit
AACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG7330.1310379348564482No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTAT6260.11190961421573885No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.11030069004969788No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGG5880.1051163788480103No Hit
AAACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT5870.10493760949622796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA406.8048394E-945.00000420
GTGCACC207.030177E-445.00000413
CTATGCG207.030177E-445.0000041
TCCATCG207.030177E-445.00000431
GCCTTAC207.030177E-445.00000435
GAAGCGA406.8048394E-945.00000444
CGTATAG207.030177E-445.00000417
TACTTGC207.030177E-445.00000423
GATCGAC207.030177E-445.00000416
CACGGCA207.030177E-445.00000427
GCGCGTT207.030177E-445.00000411
GCGAAGT207.030177E-445.00000417
GGGTCGA207.030177E-445.0000047
TAGGTTA207.030177E-445.00000413
TTAGACT207.030177E-445.00000441
CTATACG207.030177E-445.0000041
CGGTTAG207.030177E-445.00000428
GCTATCG207.030177E-445.00000418
ATTAACG207.030177E-445.0000041
GTTAGAA207.030177E-445.0000049