##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548368_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747378
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192883654589778	31.0	31.0	34.0	30.0	34.0
2	31.360579251730716	31.0	31.0	34.0	30.0	34.0
3	31.555004295015372	31.0	31.0	34.0	30.0	34.0
4	35.477253812662404	37.0	35.0	37.0	33.0	37.0
5	35.06329461129442	37.0	35.0	37.0	32.0	37.0
6	35.0416991134339	36.0	35.0	37.0	32.0	37.0
7	34.229150443283054	36.0	35.0	37.0	31.0	37.0
8	34.54839050654421	37.0	35.0	37.0	32.0	37.0
9	35.69287027447958	39.0	35.0	39.0	31.0	39.0
10	35.55308960124596	37.0	35.0	39.0	30.0	39.0
11	35.9141692690981	37.0	35.0	39.0	31.0	39.0
12	35.6318154936324	37.0	35.0	39.0	30.0	39.0
13	35.39974283428198	37.0	35.0	39.0	30.0	39.0
14	36.46217978051267	38.0	35.0	40.0	31.0	41.0
15	36.59872246707824	38.0	35.0	40.0	31.0	41.0
16	36.583380832724536	38.0	35.0	40.0	31.0	41.0
17	36.53959977414374	38.0	35.0	40.0	31.0	41.0
18	36.28682005624999	38.0	35.0	40.0	30.0	41.0
19	36.222346657247066	38.0	34.0	40.0	30.0	41.0
20	36.111488430218714	38.0	34.0	40.0	30.0	41.0
21	36.11991120958872	38.0	34.0	40.0	30.0	41.0
22	36.07823216632012	38.0	34.0	40.0	30.0	41.0
23	36.01946270829487	38.0	34.0	40.0	30.0	41.0
24	35.64068249266101	38.0	34.0	40.0	29.0	41.0
25	35.67238398775452	37.0	34.0	40.0	29.0	41.0
26	35.53911273813251	37.0	34.0	40.0	29.0	41.0
27	35.339373115076974	37.0	34.0	40.0	28.0	41.0
28	35.276942858901386	37.0	34.0	40.0	27.0	41.0
29	35.049745911707326	37.0	34.0	40.0	27.0	41.0
30	35.08553369245549	37.0	34.0	40.0	27.0	41.0
31	34.89892664755987	37.0	33.0	40.0	26.0	41.0
32	34.80361209454921	37.0	33.0	40.0	26.0	41.0
33	34.72643963295682	37.0	33.0	40.0	25.0	41.0
34	34.55151877630864	37.0	33.0	40.0	25.0	41.0
35	34.394115159932454	37.0	33.0	40.0	24.0	41.0
36	34.276075827760515	36.0	33.0	40.0	24.0	41.0
37	34.0937811923819	36.0	33.0	40.0	23.0	41.0
38	34.03359076665355	36.0	33.0	40.0	23.0	41.0
39	33.86129642563736	36.0	33.0	40.0	23.0	41.0
40	33.64933407191542	36.0	33.0	39.0	22.0	41.0
41	33.541182641180235	35.0	33.0	39.0	22.0	41.0
42	33.42258000636893	35.0	32.0	39.0	21.0	41.0
43	33.380902301111355	35.0	32.0	39.0	21.0	40.0
44	33.27845213533179	35.0	32.0	39.0	21.0	40.0
45	33.086719170219084	35.0	32.0	39.0	20.0	40.0
46	32.94664413456109	35.0	32.0	39.0	20.0	40.0
47	32.84331088150842	35.0	32.0	39.0	20.0	40.0
48	32.69050065696341	35.0	31.0	39.0	20.0	40.0
49	32.463457581036636	35.0	31.0	39.0	18.0	40.0
50	32.303232099419574	35.0	31.0	39.0	18.0	40.0
51	30.293801797751605	34.0	27.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	6.0
10	18.0
11	23.0
12	29.0
13	41.0
14	69.0
15	114.0
16	274.0
17	608.0
18	1110.0
19	1984.0
20	3214.0
21	4627.0
22	5970.0
23	7363.0
24	9038.0
25	9912.0
26	11321.0
27	13358.0
28	16110.0
29	19520.0
30	23979.0
31	30457.0
32	39181.0
33	53273.0
34	80551.0
35	78862.0
36	75945.0
37	96125.0
38	108538.0
39	55754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.902809555539502	33.37788910029463	19.359815247438377	26.35948609672749
2	22.34010099307178	35.62454340373947	19.324491756514107	22.71086384667464
3	21.215235128676518	34.60390859779121	20.75522694004908	23.42562933348319
4	20.773691492123127	35.389187265346315	17.278672907150064	26.55844833538049
5	17.472015499519657	39.90443925296169	16.207862687957096	26.41568255956156
6	19.403300605583787	38.853699199066604	20.063073839476143	21.679926355873466
7	77.74058107142571	14.11160082314438	4.405133680680994	3.7426844247489224
8	79.97519327569181	12.107126514293972	3.519504186636481	4.3981760233777285
9	74.44465852620762	15.063328061569914	6.378030929462734	4.113982482759728
10	42.98025898541301	32.29155795327131	10.899437767769456	13.828745293546238
11	34.1350695364327	31.210712651429397	17.964403554827676	16.689814257310225
12	31.685706563479254	29.07083162736928	23.72293538209581	15.520526427055653
13	24.8967724498179	35.91382138623294	20.451766040745113	18.737640123204056
14	17.933361699166955	38.19232035195042	25.151262145795034	18.723055803087593
15	16.700518345469092	32.225594009992264	31.677812298462086	19.39607534607655
16	18.697098389302333	31.084805814460687	30.775323865567355	19.442771930669622
17	18.42641875998491	29.958468137943584	24.151232709552595	27.463880392518913
18	21.68835582529858	32.61950445423869	24.848470252000997	20.84366946846174
19	24.81354816438268	33.85502382997627	22.352544495556465	18.97888351008459
20	26.62039824559995	33.08928012331109	20.435174704098863	19.85514692699009
21	23.63235203605137	32.075602974666104	22.989437741009237	21.302607248273297
22	22.401917102189252	32.387894746701136	22.816834319447455	22.393353831662157
23	23.00161364128995	33.429268723457206	20.27822601146943	23.290891623783413
24	20.965294670166905	32.49600603710572	25.11419924054494	21.42450005218243
25	21.046645740174316	32.11333488542611	23.441953067925468	23.3980663064741
26	21.390514572278015	34.84970122213927	21.363219147472897	22.396565058109818
27	18.8319699001041	35.67485261808616	23.80950469508067	21.683672786729073
28	19.76242276331388	34.72486479398644	26.548145650527577	18.9645667921721
29	20.635876357077677	33.47181747388871	24.60174101993904	21.29056514909457
30	22.533443585441372	32.35016283594111	23.512466248672023	21.60392732994549
31	25.915534040338358	33.29252399722764	19.695656013422926	21.096285949011076
32	26.79795230793521	32.8789447909893	21.198509990928287	19.124592910147207
33	26.555772313340775	31.53986336231465	21.440154781114778	20.464209543229796
34	22.70015975851577	34.231673932066506	21.939232891522096	21.128933417895627
35	22.335417954502272	33.02692880978568	23.37371450591267	21.263938729799378
36	27.387212361081005	31.648911260433145	22.42118446087522	18.542691917610636
37	23.682527449296074	34.75537144523923	22.284974939053598	19.277126166411108
38	25.78828919234979	31.912365630243333	21.076884789223126	21.222460388183755
39	24.63291667670175	30.81051355538964	23.02623304405535	21.530336723853257
40	25.70024806724308	30.541439539296043	22.078787440893365	21.679524952567508
41	20.559609728945727	30.026439097752412	26.023110126335002	23.390841046966862
42	25.992068270674277	30.52805942909746	22.2025534602303	21.277318839997967
43	25.477201630232628	29.98937619250232	22.949832614821418	21.583589562443635
44	23.040951165273796	31.96628747434364	22.38626237325691	22.606498987125658
45	24.36611727934191	33.78611626245353	21.304212861497128	20.543553596707422
46	22.942072150906235	31.091897272865936	26.00143434781329	19.96459622841454
47	24.32825156747991	31.48018807082895	22.83355945719569	21.35800090449545
48	24.36745529036177	27.964697917252046	26.00919481172847	21.658651980657712
49	22.444465852620763	28.480367364305614	25.715902796175428	23.359263986898195
50	21.72301031071292	30.8127881741234	23.652556002451234	23.811645512712442
51	22.3114675572468	30.570608179528968	23.384819997377498	23.733104265846734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18891.0
1	15127.5
2	11364.0
3	7481.5
4	3599.0
5	3166.5
6	2734.0
7	2811.0
8	2888.0
9	2996.0
10	3104.0
11	3271.5
12	3439.0
13	3678.0
14	3917.0
15	3739.0
16	3561.0
17	3646.0
18	3731.0
19	3404.5
20	3078.0
21	3060.0
22	3042.0
23	3092.0
24	3142.0
25	4432.0
26	6579.5
27	7437.0
28	8611.0
29	9785.0
30	10094.0
31	10403.0
32	11987.5
33	13572.0
34	15064.5
35	16557.0
36	17731.5
37	18906.0
38	20393.0
39	21880.0
40	24596.5
41	27313.0
42	33764.5
43	40216.0
44	48211.0
45	56206.0
46	55885.5
47	55565.0
48	55295.5
49	55026.0
50	52197.5
51	49369.0
52	46805.5
53	44242.0
54	41624.5
55	39007.0
56	35779.0
57	32551.0
58	32189.5
59	31828.0
60	32974.0
61	34120.0
62	32833.0
63	31546.0
64	28455.0
65	25364.0
66	20365.5
67	15367.0
68	13940.0
69	12513.0
70	10769.5
71	9026.0
72	7576.5
73	6127.0
74	5487.0
75	3690.0
76	2533.0
77	1937.5
78	1342.0
79	1202.0
80	1062.0
81	843.0
82	624.0
83	451.0
84	278.0
85	235.5
86	193.0
87	175.5
88	158.0
89	120.5
90	83.0
91	67.5
92	52.0
93	48.0
94	44.0
95	31.0
96	18.0
97	10.0
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	747378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.114564468279347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.86433661892514	19.500082585817125
2	8.489500764609609	4.094412269833704
3	2.9481949307520496	2.13283310168024
4	1.4670987346411646	1.4151378807134167
5	0.8508770502491161	1.0259264741405838
6	0.544156921419383	0.7873264297456878
7	0.39356499319260174	0.6643453880560641
8	0.2794405409956054	0.5390869552711508
9	0.23782790001197884	0.5161604604474923
>10	2.10295519208544	11.691016585212443
>50	0.865770853824541	15.428075418909993
>100	0.9477373251925449	36.540482046986526
>500	0.0034152696403334957	0.5151476631894625
>1k	0.004553692853777994	2.9252096590009686
>5k	0.0	0.0
>10k+	5.692116067222493E-4	2.2247570809951527
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16208	2.1686482609870774	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4093	0.5476479104281903	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3169	0.4240156921932409	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC	3048	0.40782575885294997	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGT	3005	0.40207231146755723	No Hit
AATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	2451	0.327946500967382	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	2236	0.2991792640404186	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1922	0.25716571801685356	No Hit
GCAAACTAACCAACCATATAATTAACCTCCAACCCTCACACACACGAGAAC	1387	0.18558212845440997	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	898	0.12015338958331662	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.676022039717519E-4	0.0	0.0	0.45157871920233134	0.0
2	2.676022039717519E-4	0.0	0.0	0.5475141093262044	0.0
3	2.676022039717519E-4	0.0	0.0	0.71409648129862	0.0
4	2.676022039717519E-4	0.0	0.0	1.7028866249742434	0.0
5	2.676022039717519E-4	0.0	0.0	1.8334765005124583	0.0
6	2.676022039717519E-4	0.0	0.0	2.427820995533719	0.0
7	2.676022039717519E-4	0.0	0.0	2.911378178110675	0.0
8	2.676022039717519E-4	0.0	0.0	3.206677210193503	0.0
9	2.676022039717519E-4	0.0	0.0	4.153453807845562	0.0
10	2.676022039717519E-4	0.0	0.0	4.835973229075515	0.0
11	2.676022039717519E-4	0.0	0.0	5.931804254339839	0.0
12	2.676022039717519E-4	0.0	0.0	6.30096149471887	0.0
13	2.676022039717519E-4	0.0	0.0	6.481459181297817	0.0
14	2.676022039717519E-4	0.0	0.0	6.6769425912991816	0.0
15	2.676022039717519E-4	0.0	0.0	6.8222505880558435	0.0
16	2.676022039717519E-4	0.0	0.0	7.057874328652971	0.0
17	2.676022039717519E-4	0.0	0.0	7.433186419723353	0.0
18	2.676022039717519E-4	0.0	0.0	7.882758122395896	0.0
19	2.676022039717519E-4	0.0	0.0	8.021242262951278	0.0
20	2.676022039717519E-4	0.0	0.0	8.249908346245139	0.0
21	2.676022039717519E-4	0.0	0.0	8.437631292331323	0.0
22	2.676022039717519E-4	0.0	0.0	8.642748381675672	0.0
23	2.676022039717519E-4	0.0	0.0	8.847731669918034	0.0
24	2.676022039717519E-4	0.0	0.0	8.984610197249584	0.0
25	2.676022039717519E-4	0.0	0.0	9.10770721107659	0.0
26	2.676022039717519E-4	0.0	0.0	9.21220587172756	0.0
27	2.676022039717519E-4	0.0	0.0	9.36728134892919	0.0
28	2.676022039717519E-4	0.0	0.0	9.522624428334792	0.0
29	2.676022039717519E-4	0.0	0.0	9.642911619020094	0.0
30	2.676022039717519E-4	0.0	0.0	9.825416322128829	0.0
31	2.676022039717519E-4	0.0	0.0	9.945837313916117	0.0
32	2.676022039717519E-4	0.0	0.0	10.072011753088798	0.0
33	2.676022039717519E-4	0.0	0.0	10.204474844054815	0.0
34	2.676022039717519E-4	0.0	0.0	10.330247879921538	0.0
35	2.676022039717519E-4	0.0	0.0	10.473147456842455	0.0
36	2.676022039717519E-4	0.0	0.0	10.597314879485348	0.0
37	4.014033059576279E-4	0.0	0.0	10.728439959431507	0.0
38	4.014033059576279E-4	0.0	0.0	10.8667902988849	0.0
39	4.014033059576279E-4	0.0	0.0	11.019591157352773	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.0317014E-4	45.0	14
AACGTTC	25	3.8894916E-5	45.0	17
CTTAACA	20	7.0317014E-4	45.0	22
GTCATAT	20	7.0317014E-4	45.0	22
TTCTACG	20	7.0317014E-4	45.0	1
AACCGTA	20	7.0317014E-4	45.0	32
GTACCGT	20	7.0317014E-4	45.0	27
TGCCCGC	35	1.2111741E-7	45.0	19
TCCGCGT	20	7.0317014E-4	45.0	32
GTCGATT	20	7.0317014E-4	45.0	19
ATTAGCG	50	2.1827873E-11	45.0	1
ATTAGAC	20	7.0317014E-4	45.0	29
ATCTAGC	25	3.8894916E-5	45.0	43
GTCGAAG	20	7.0317014E-4	45.0	37
GGTCGAT	20	7.0317014E-4	45.0	18
CGAGTAT	20	7.0317014E-4	45.0	33
CGAGTAA	25	3.8894916E-5	45.0	16
GTTTCGA	20	7.0317014E-4	45.0	35
CGAAACG	45	3.8380676E-10	45.0	1
TCCGAAC	20	7.0317014E-4	45.0	39
>>END_MODULE