FastQCFastQC Report
Sat 18 Jun 2016
SRR3548366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637677
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226533.5524254442296024No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55650.8726988741949293No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43310.6791839755863862No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT30280.4748485518530542No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25810.40475036734898706No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT10910.17108975233543No Hit
AATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT10550.16544426096597495No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC9460.14835096765290265No Hit
AAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT8160.12796447104098155No Hit
GGAGGAGGAGGAAGAGGAGGAGGAGGAGAACAAGACGAACAAGAAAGAAGA6390.10020747180782748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATT351.2107921E-745.00000439
TTAGCGT351.2107921E-745.00000434
TTTCGTC551.8189894E-1245.00000416
TAGCGTA351.2107921E-745.00000435
CGTTCAC351.2107921E-745.00000421
CGTTACG351.2107921E-745.0000041
TCTACGA551.8189894E-1245.00000422
GCTTACG351.2107921E-745.0000041
ACGTGAC351.2107921E-745.00000421
CCGACCC351.2107921E-745.00000421
CGTCGCA351.2107921E-745.00000412
AACTCGC351.2107921E-745.0000041
CGGTCGG351.2107921E-745.00000413
CAATCCG351.2107921E-745.0000041
ATTTCGC551.8189894E-1245.00000417
TCGCCCA207.030922E-445.018
CTATGCG406.8066583E-945.015
CTGTCGT207.030922E-445.024
CTGTCCG207.030922E-445.01
GTCGCAC207.030922E-445.035