##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548366_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637677
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.984733650421767	31.0	31.0	33.0	28.0	34.0
2	31.15344288722974	31.0	31.0	34.0	28.0	34.0
3	31.37916845048512	31.0	31.0	34.0	30.0	34.0
4	35.3588917273165	37.0	35.0	37.0	33.0	37.0
5	34.92754011827304	35.0	35.0	37.0	32.0	37.0
6	34.87292155746562	35.0	35.0	37.0	32.0	37.0
7	34.10258798733528	35.0	35.0	37.0	31.0	37.0
8	34.46949317601231	36.0	35.0	37.0	32.0	37.0
9	35.51813222054426	38.0	35.0	39.0	30.0	39.0
10	35.16939140034845	37.0	34.0	39.0	29.0	39.0
11	35.597319646153146	37.0	35.0	39.0	30.0	39.0
12	35.435123110916656	37.0	35.0	39.0	30.0	39.0
13	35.06380189343508	37.0	34.0	39.0	28.0	39.0
14	36.07328475074372	38.0	34.0	40.0	30.0	41.0
15	36.28850813185986	38.0	34.0	40.0	30.0	41.0
16	36.31039382006878	38.0	35.0	40.0	31.0	41.0
17	36.290996852638564	38.0	35.0	40.0	31.0	41.0
18	36.04409128759544	37.0	35.0	40.0	30.0	41.0
19	35.86078688740538	37.0	34.0	40.0	30.0	41.0
20	35.75483042355299	37.0	34.0	40.0	30.0	41.0
21	35.74631357254535	37.0	34.0	40.0	30.0	41.0
22	35.723952722146166	37.0	34.0	40.0	30.0	41.0
23	35.58667946311377	37.0	34.0	40.0	29.0	41.0
24	35.1867638318459	37.0	33.0	40.0	27.0	41.0
25	35.26115572617485	37.0	34.0	40.0	27.0	41.0
26	35.14038455205378	37.0	33.0	40.0	27.0	41.0
27	34.94848645944577	37.0	33.0	40.0	27.0	41.0
28	34.84034236768772	36.0	33.0	40.0	26.0	41.0
29	34.5831855312329	36.0	33.0	40.0	25.0	41.0
30	34.623869137510056	36.0	33.0	40.0	25.0	41.0
31	34.30956267828383	36.0	33.0	40.0	24.0	41.0
32	34.200891673997965	36.0	33.0	40.0	24.0	41.0
33	34.110236059948846	36.0	33.0	40.0	24.0	41.0
34	34.047122602822434	36.0	33.0	40.0	23.0	41.0
35	33.91097216929574	36.0	33.0	40.0	23.0	41.0
36	33.69394536732546	35.0	33.0	39.0	22.0	41.0
37	33.46193292215338	35.0	32.0	39.0	21.0	41.0
38	33.33222462155605	35.0	32.0	39.0	21.0	40.0
39	33.200604694853354	35.0	32.0	39.0	21.0	40.0
40	33.03348246839701	35.0	31.0	39.0	20.0	40.0
41	33.07004172959037	35.0	32.0	39.0	20.0	40.0
42	32.95816847714438	35.0	32.0	39.0	19.0	40.0
43	32.921668807248814	35.0	32.0	39.0	19.0	40.0
44	32.85369081839239	35.0	32.0	39.0	18.0	40.0
45	32.684043802740256	35.0	31.0	39.0	19.0	40.0
46	32.55546303222478	35.0	31.0	39.0	18.0	40.0
47	32.51134038078839	35.0	31.0	39.0	18.0	40.0
48	32.34434831427196	35.0	31.0	39.0	18.0	40.0
49	32.153858458122215	35.0	31.0	39.0	15.0	40.0
50	31.97736942056872	35.0	31.0	39.0	15.0	40.0
51	30.10101352252002	34.0	27.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	10.0
11	12.0
12	15.0
13	32.0
14	46.0
15	110.0
16	212.0
17	488.0
18	1053.0
19	2040.0
20	3315.0
21	4663.0
22	6422.0
23	8024.0
24	9053.0
25	9840.0
26	11014.0
27	12393.0
28	14753.0
29	17974.0
30	22114.0
31	27634.0
32	35563.0
33	47329.0
34	71841.0
35	73189.0
36	63199.0
37	75985.0
38	80378.0
39	38968.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.5392024488887	36.91790671452789	19.189338803187194	24.353552033396216
2	19.23465955334754	37.91167001475669	20.122726709603764	22.730943722292007
3	18.67199224685852	38.940403997635165	19.319969200708194	23.06763455479812
4	17.887739404118385	39.50432585776185	17.81215254744957	24.7957821906702
5	16.436220845349606	42.354044445698996	16.961565181118342	24.248169527833056
6	17.769340904564537	43.57927289207545	18.187734542723042	20.46365166063697
7	74.94358429110662	17.120893493100738	3.9491780321385277	3.9863441836541074
8	77.87108520457849	15.13979648003613	3.31421707227954	3.674901243105836
9	71.10543425590072	20.230304684032827	5.753696620702957	2.9105644393635024
10	36.19230425434821	44.274922884156084	9.288715133210074	10.244057728285636
11	28.83246533903528	33.008403941180255	25.17732331572254	12.981807404061932
12	30.682147858555346	31.083134564991365	24.005099760537075	14.22961781591621
13	20.825747204305628	41.97046467098547	21.646852560151927	15.556935564556978
14	14.695057215486838	45.21442673955624	24.86556673676485	15.224949308192079
15	12.910454665920208	37.708275506251596	33.128684271190586	16.25258555663761
16	14.285915910406052	35.09551073662685	32.323260835815	18.2953125171521
17	14.525222016789064	34.04842890679764	24.8268323932022	26.599516683211093
18	18.706649291098785	36.209868005275396	25.099384170983114	19.9840985326427
19	18.68155821834565	39.282269863896616	23.583883376693844	18.452288541063893
20	21.273622852949064	36.75465792242781	22.65598414244202	19.315735082181103
21	17.73217475304896	38.3258295343881	24.097623091314254	19.844372621248688
22	18.08219521795517	36.05806701511894	23.235117465425287	22.624620301500602
23	17.275046771327805	38.178733120372854	20.80034249314308	23.745877615156264
24	15.641774754303512	37.61512489865559	27.198252406782746	19.544847940258155
25	14.931070118571	37.95259982718523	25.438584110764538	21.677745943479223
26	17.833009501675612	41.72363124277651	21.033846288951928	19.40951296659594
27	15.325627237614029	40.63703097336112	23.395543511840632	20.64179827718422
28	15.707795639485195	38.93632669831278	27.50185438709566	17.85402327510636
29	16.760522960683858	37.055750795465414	26.315673922691268	19.868052321159457
30	18.922902974389856	37.87528795926464	23.45607572485757	19.74573334148793
31	21.14361973224689	40.9862673422438	18.60252133917328	19.26759158633603
32	21.931165778285873	39.186766968229996	20.224345554253954	18.657721699230176
33	20.570445539042492	38.687454620442644	20.15032688963221	20.591772950882657
34	18.287157918507333	39.563917155550534	20.73620343841788	21.412721487524248
35	18.822381864172613	37.5887792722648	22.167962777393573	21.420876086169017
36	23.68142492202165	35.59748901089423	22.798846437930177	17.922239629153943
37	18.78490207424762	39.51279409481603	22.517355965480956	19.184947865455396
38	18.65474213434074	38.27611784649596	22.100687338574232	20.968452680589074
39	19.391165119645212	36.71686449409341	23.744152603904485	20.1478177823569
40	20.21760232845155	35.77375379698499	21.5267290493463	22.481914825217157
41	17.62287176736812	32.750122711027686	25.878305160763208	23.748700360840992
42	21.353287008940264	33.273271577930515	22.894035695187377	22.47940571794184
43	20.436208299813227	33.47243196790852	24.61167644434408	21.479683287934172
44	18.877895862638923	35.741605860020044	22.737059671275585	22.643438606065452
45	19.16095452713521	39.18425786095469	22.037332379872566	19.617455232037535
46	19.740087850118478	36.87274278357225	24.78872532645838	18.598444039850897
47	20.064546784657438	34.350149056654075	23.447293849393972	22.13801030929452
48	20.11817895266726	32.52649852511538	27.310534957353017	20.044787564864343
49	20.82355173543973	31.413238990899785	25.788761394875465	21.97444787878503
50	18.992530701279804	33.0758361992043	23.97953823016982	23.95209486934608
51	18.231487100836315	34.17639337783862	24.371899880346948	23.22021964097811
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26079.0
1	20479.0
2	14879.0
3	9693.0
4	4507.0
5	3999.0
6	3491.0
7	3619.5
8	3748.0
9	4287.5
10	4827.0
11	5031.0
12	5235.0
13	5541.0
14	5847.0
15	5707.0
16	5567.0
17	5416.0
18	5265.0
19	5013.5
20	4762.0
21	4696.5
22	4631.0
23	4834.0
24	5037.0
25	4721.5
26	4598.5
27	4791.0
28	6604.5
29	8418.0
30	8747.0
31	9076.0
32	11226.5
33	13377.0
34	15336.5
35	17296.0
36	18348.0
37	19400.0
38	21122.0
39	22844.0
40	25582.0
41	28320.0
42	35095.0
43	41870.0
44	49036.5
45	56203.0
46	58410.0
47	60617.0
48	58456.0
49	56295.0
50	53658.0
51	51021.0
52	44547.0
53	38073.0
54	32473.5
55	26874.0
56	23063.5
57	19253.0
58	17635.5
59	16018.0
60	15473.5
61	14929.0
62	13335.5
63	11742.0
64	9692.5
65	7643.0
66	6325.5
67	5008.0
68	4531.0
69	4054.0
70	3143.0
71	2232.0
72	2135.0
73	2038.0
74	1499.0
75	702.5
76	445.0
77	316.5
78	188.0
79	169.5
80	151.0
81	123.0
82	95.0
83	76.0
84	57.0
85	44.5
86	32.0
87	23.0
88	14.0
89	17.0
90	20.0
91	28.0
92	36.0
93	19.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	637677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.35584152660115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.46694067758612	26.03575580694392
2	8.516000373432222	5.5108471704649835
3	3.1930167895946546	3.0993823570770425
4	1.6079458375424576	2.0810576281152686
5	0.9832948736130822	1.5907666552272097
6	0.6636515700755736	1.2883803018147177
7	0.4835116845705887	1.0951099209558148
8	0.35589844063077014	0.9212314835690932
9	0.286314898096655	0.8337563522568292
>10	2.434737751553566	17.18006128153023
>50	0.6140906814738232	14.097872717910024
>100	0.3886774755030791	19.269507899758565
>500	0.002466227636440857	0.5640042812114092
>1k	0.002466227636440857	1.928849955495499
>5k	4.932455272881715E-4	0.8881391694797659
>10k+	4.932455272881715E-4	3.6152770181896026
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22653	3.5524254442296024	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5565	0.8726988741949293	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4331	0.6791839755863862	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	3028	0.4748485518530542	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2581	0.40475036734898706	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT	1091	0.17108975233543	No Hit
AATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	1055	0.16544426096597495	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	946	0.14835096765290265	No Hit
AAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	816	0.12796447104098155	No Hit
GGAGGAGGAGGAAGAGGAGGAGGAGGAGAACAAGACGAACAAGAAAGAAGA	639	0.10020747180782748	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.704576141212557E-4	0.0	0.0	0.6663248008004052	0.0
2	4.704576141212557E-4	0.0	0.0	0.8411782140488053	0.0
3	4.704576141212557E-4	0.0	0.0	1.0693501568976143	0.0
4	4.704576141212557E-4	0.0	0.0	2.299596817824698	0.0
5	4.704576141212557E-4	0.0	0.0	2.492013982000292	0.0
6	4.704576141212557E-4	0.0	0.0	3.4155222785203168	0.0
7	4.704576141212557E-4	0.0	0.0	4.140183823471758	0.0
8	4.704576141212557E-4	0.0	0.0	4.671016831405241	0.0
9	4.704576141212557E-4	0.0	0.0	5.72452824862744	0.0
10	4.704576141212557E-4	0.0	0.0	7.1020281427744765	0.0
11	4.704576141212557E-4	0.0	0.0	8.511519154681759	0.0
12	4.704576141212557E-4	0.0	0.0	9.16561205751501	0.0
13	4.704576141212557E-4	0.0	0.0	9.476270902039747	0.0
14	4.704576141212557E-4	0.0	0.0	9.751488606300683	0.0
15	4.704576141212557E-4	0.0	0.0	9.99691066166727	0.0
16	4.704576141212557E-4	0.0	0.0	10.426281644155269	0.0
17	4.704576141212557E-4	0.0	0.0	11.020626429995122	0.0
18	4.704576141212557E-4	0.0	0.0	11.764733556330242	0.0
19	4.704576141212557E-4	0.0	0.0	12.0736673896032	0.0
20	4.704576141212557E-4	0.0	0.0	12.422276481667051	0.0
21	4.704576141212557E-4	0.0	0.0	12.765867359180275	0.0
22	4.704576141212557E-4	0.0	0.0	13.165285873569221	0.0
23	4.704576141212557E-4	0.0	0.0	13.561097546249904	0.0
24	4.704576141212557E-4	0.0	0.0	13.825651544590757	0.0
25	4.704576141212557E-4	0.0	0.0	14.045825707999505	0.0
26	4.704576141212557E-4	0.0	0.0	14.281995430288374	0.0
27	4.704576141212557E-4	0.0	0.0	14.519890163829023	0.0
28	4.704576141212557E-4	0.0	0.0	14.76327356953442	0.0
29	4.704576141212557E-4	0.0	0.0	15.030023036741172	0.0
30	4.704576141212557E-4	0.0	0.0	15.362009293106071	0.0
31	4.704576141212557E-4	0.0	0.0	15.641931573508218	0.0
32	4.704576141212557E-4	0.0	0.0	15.887824086488928	0.0
33	4.704576141212557E-4	0.0	0.0	16.153789457672143	0.0
34	4.704576141212557E-4	0.0	0.0	16.409404761344693	0.0
35	4.704576141212557E-4	0.0	0.0	16.695913448344537	0.0
36	4.704576141212557E-4	0.0	0.0	16.960624265890097	0.0
37	4.704576141212557E-4	0.0	0.0	17.24305565356756	0.0
38	4.704576141212557E-4	0.0	0.0	17.527839329315626	0.0
39	4.704576141212557E-4	0.0	0.0	17.793647881294135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTATT	35	1.2107921E-7	45.000004	39
TTAGCGT	35	1.2107921E-7	45.000004	34
TTTCGTC	55	1.8189894E-12	45.000004	16
TAGCGTA	35	1.2107921E-7	45.000004	35
CGTTCAC	35	1.2107921E-7	45.000004	21
CGTTACG	35	1.2107921E-7	45.000004	1
TCTACGA	55	1.8189894E-12	45.000004	22
GCTTACG	35	1.2107921E-7	45.000004	1
ACGTGAC	35	1.2107921E-7	45.000004	21
CCGACCC	35	1.2107921E-7	45.000004	21
CGTCGCA	35	1.2107921E-7	45.000004	12
AACTCGC	35	1.2107921E-7	45.000004	1
CGGTCGG	35	1.2107921E-7	45.000004	13
CAATCCG	35	1.2107921E-7	45.000004	1
ATTTCGC	55	1.8189894E-12	45.000004	17
TCGCCCA	20	7.030922E-4	45.0	18
CTATGCG	40	6.8066583E-9	45.0	15
CTGTCGT	20	7.030922E-4	45.0	24
CTGTCCG	20	7.030922E-4	45.0	1
GTCGCAC	20	7.030922E-4	45.0	35
>>END_MODULE