##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	707892
Sequences flagged as poor quality	0
Sequence length	46
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.843500138439197	31.0	30.0	33.0	28.0	34.0
2	31.163106801602503	31.0	31.0	34.0	28.0	34.0
3	31.345520220598623	31.0	31.0	34.0	28.0	34.0
4	35.367096958293075	37.0	35.0	37.0	33.0	37.0
5	34.902329733914215	35.0	35.0	37.0	32.0	37.0
6	35.01508563453182	35.0	35.0	37.0	32.0	37.0
7	34.913950150587944	36.0	35.0	37.0	32.0	37.0
8	35.343979025049016	37.0	35.0	37.0	33.0	37.0
9	36.63885875246506	39.0	35.0	39.0	32.0	39.0
10	35.87161035864228	37.0	35.0	39.0	31.0	39.0
11	36.1440163188735	37.0	35.0	39.0	31.0	39.0
12	35.86559249151001	37.0	35.0	39.0	31.0	39.0
13	35.380038197917195	37.0	35.0	39.0	30.0	39.0
14	36.50897735812808	38.0	35.0	40.0	31.0	41.0
15	36.536773971170746	38.0	35.0	40.0	31.0	41.0
16	36.481035242664134	38.0	35.0	40.0	31.0	41.0
17	36.47265967124929	38.0	35.0	40.0	31.0	41.0
18	36.22673797697954	37.0	35.0	40.0	31.0	41.0
19	36.31171139100315	38.0	35.0	40.0	31.0	41.0
20	36.063714521424174	37.0	34.0	40.0	30.0	41.0
21	36.132342221694834	38.0	34.0	40.0	30.0	41.0
22	36.234462319110825	38.0	34.0	40.0	31.0	41.0
23	36.03026450362485	38.0	34.0	40.0	31.0	41.0
24	35.59067343606087	37.0	34.0	40.0	29.0	41.0
25	35.77733467817124	37.0	34.0	40.0	30.0	41.0
26	35.78014584145604	37.0	34.0	40.0	30.0	41.0
27	35.65906098670418	37.0	34.0	40.0	30.0	41.0
28	35.61229396574619	37.0	34.0	40.0	29.0	41.0
29	35.280387403728255	37.0	34.0	40.0	27.0	41.0
30	35.31123956761766	37.0	34.0	40.0	28.0	41.0
31	35.09001655619784	37.0	34.0	40.0	27.0	41.0
32	35.01525938985043	37.0	34.0	40.0	27.0	41.0
33	34.97902646166364	37.0	34.0	40.0	27.0	41.0
34	34.918905708780436	37.0	34.0	40.0	26.0	41.0
35	34.69921824233075	37.0	33.0	40.0	25.0	41.0
36	34.633870703440635	37.0	33.0	40.0	25.0	41.0
37	34.41647030903019	36.0	33.0	40.0	24.0	41.0
38	34.172469811779195	36.0	33.0	40.0	23.0	41.0
39	34.119362840659306	36.0	33.0	40.0	23.0	41.0
40	33.97161148875817	36.0	33.0	40.0	23.0	41.0
41	34.043618235550056	36.0	33.0	40.0	23.0	41.0
42	33.94156170715307	36.0	33.0	40.0	23.0	41.0
43	33.91315624417284	36.0	33.0	40.0	23.0	41.0
44	33.821023545964636	36.0	33.0	40.0	22.0	41.0
45	33.596983720680555	35.0	33.0	40.0	22.0	41.0
46	32.668402807207876	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	6.0
11	8.0
12	13.0
13	15.0
14	27.0
15	52.0
16	109.0
17	229.0
18	447.0
19	886.0
20	1629.0
21	2760.0
22	3989.0
23	5378.0
24	7086.0
25	8546.0
26	9987.0
27	11322.0
28	12512.0
29	15526.0
30	19516.0
31	25358.0
32	33698.0
33	46892.0
34	76906.0
35	88000.0
36	71248.0
37	89605.0
38	110459.0
39	65678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.270171156052054	36.11497234041351	19.090765257977203	25.524091245557234
2	19.718827165725845	36.652483712204685	19.542105292897787	24.08658382917168
3	18.68321721392529	38.55093714860459	19.323569132014487	23.442276505455634
4	17.32128629790985	39.20767574714787	18.19062794889616	25.28041000604612
5	16.69760358924808	41.37481423720003	17.355189774711395	24.5723923988405
6	18.135817328066995	42.861905488407835	18.433461601487235	20.56881558203794
7	75.97246472625768	15.169686901391739	4.583326270109	4.274522102241585
8	78.2222429410136	14.074180807241781	3.7754346708254936	3.9281415809191236
9	71.77337785989954	18.39786295084561	6.703988744045702	3.1247704452091565
10	35.40780232012793	43.864742079300235	9.744141761737666	10.983313838834173
11	28.00723839229713	32.43842281025919	26.199618020828037	13.354720776615641
12	30.029298254536002	30.68504800167257	24.877664954541086	14.407988789250336
13	20.812355557062375	41.088753651686986	22.27585563899578	15.823035152254864
14	14.506732665434841	44.302661987986866	25.523667452097214	15.666937894481078
15	12.720301966966712	37.18505082696231	33.228656348708554	16.865990857362423
16	13.970351409537049	35.08091629796636	32.21338848298893	18.735343809507665
17	14.0898611652625	33.83566984794291	25.314737276307685	26.75973171048691
18	18.39291869381205	35.48563905228481	25.53694631384448	20.584495940058652
19	18.445045289394425	39.26757188949727	24.17911206794257	18.108270753165737
20	20.80303210094195	36.279262938414334	23.298186729049064	19.61951823159465
21	17.319308595096427	38.32957569798783	24.516451662117948	19.834664044797794
22	17.563413628067558	36.069767704678114	23.66603945234584	22.70077921490849
23	17.205025625377882	37.912562933328815	20.952207398868754	23.93020404242455
24	15.194266922072858	37.362479022223724	27.200476908907007	20.242777146796403
25	14.755358161979512	37.72778898476039	25.610827640374524	21.90602521288558
26	17.953162346798663	41.285111288162604	21.339978414786437	19.4217479502523
27	15.249642600848718	40.02771609228527	23.37757737055935	21.34506393630667
28	15.82656676442169	38.511100563362774	27.46096862233222	18.201364049883313
29	17.082408050945624	36.94843281178485	26.227447124702643	19.74171201256689
30	18.838608149265706	37.7135212716064	23.163279144276245	20.284591434851645
31	20.8537460516576	41.16998073152402	18.552123770292646	19.42414944652574
32	21.95998824679471	39.20951218547462	20.042887898153953	18.787611669576716
33	20.346465280014467	38.283664739819066	20.304933520932572	21.064936459233895
34	18.0438541472428	39.60364010329259	20.504116447141655	21.848389302322953
35	18.79410983596368	37.39228582891175	22.025534968611034	21.788069366513536
36	23.68835924124019	35.31654546173711	22.81068298554017	18.184412311482543
37	18.70511320935962	39.73953653947213	22.203952015279167	19.351398235889093
38	18.7551208376419	38.34483226254852	22.352986048719295	20.54706085109028
39	19.203353053855672	36.81352522701203	23.717459725494848	20.265661993637448
40	20.109564735863664	35.682844275680466	21.453131268611596	22.75445971984427
41	17.330609754030277	33.04148090386669	25.73245636340007	23.895452978702966
42	21.19786634119329	33.583936532691425	23.07399998869884	22.14419713741644
43	20.064642629101613	33.31242618930571	25.00409667011352	21.61883451147915
44	19.214230419329503	35.202262492018555	22.642013188452477	22.941493900199468
45	19.035107050228	38.52861735971024	22.556265645041897	19.88000994501986
46	19.339955812468567	36.322913664796324	25.473801088301606	18.863329434433503
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32575.0
1	25629.0
2	18683.0
3	12232.0
4	5781.0
5	5232.5
6	4684.0
7	4684.0
8	4647.0
9	4610.0
10	4936.0
11	5262.0
12	5419.0
13	5576.0
14	5635.0
15	5694.0
16	5634.0
17	5574.0
18	5493.5
19	5413.0
20	5413.0
21	5436.0
22	5459.0
23	5605.5
24	5752.0
25	5613.0
26	5474.0
27	6455.5
28	7437.0
29	8335.5
30	9234.0
31	9234.0
32	10855.0
33	12476.0
34	14222.0
35	15968.0
36	19414.0
37	22860.0
38	25440.5
39	28021.0
40	32406.5
41	36792.0
42	43794.0
43	50796.0
44	50796.0
45	58300.0
46	65804.0
47	73987.5
48	82171.0
49	77364.0
50	72557.0
51	63996.5
52	55436.0
53	45412.0
54	35388.0
55	30280.0
56	25172.0
57	25172.0
58	22949.0
59	20726.0
60	18977.5
61	17229.0
62	15887.0
63	14545.0
64	11866.0
65	9187.0
66	7695.0
67	6203.0
68	5029.0
69	3855.0
70	3855.0
71	3230.0
72	2605.0
73	2148.5
74	1692.0
75	1196.0
76	700.0
77	468.0
78	236.0
79	179.0
80	122.0
81	122.0
82	82.5
83	43.0
84	38.5
85	34.0
86	35.5
87	37.0
88	25.0
89	13.0
90	12.5
91	12.0
92	6.0
93	0.0
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	707892.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.39174782149045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12407949451372	20.09047509162298
2	9.984129070622117	5.269972332981414
3	3.789804547518542	3.0005869773254124
4	1.928578747177545	2.03594255797583
5	1.2177757449383737	1.6069615181770616
6	0.8798130629400517	1.393188269230017
7	0.6445173886896276	1.1906958272203536
8	0.5160294365240207	1.0895135005766212
9	0.41172459397302713	0.9779518490437511
>10	3.204448210018307	18.090536200846547
>50	0.7418812228971033	13.96910817676388
>100	0.5464509733240606	22.682856952689882
>500	0.006998879461293387	1.2482313336134239
>1k	0.001615126029529243	0.7753669194682361
>5k	0.001615126029529243	2.5608279255224518
>10k+	5.383753431764144E-4	4.01778456694215
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28277	3.9945358896554843	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7158	1.0111711956061094	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	5452	0.7701739813417866	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5413	0.7646646663615354	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3176	0.44865600967379204	No Hit
AAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT	1197	0.16909359054771067	No Hit
AATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT	1084	0.15313070355364944	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTAT	949	0.1340599978527798	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGT	871	0.12304136789227735	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	838	0.11837963983206477	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	811	0.11456549869189084	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8902487950139287	0.0
2	0.0	0.0	0.0	1.1426884326987732	0.0
3	0.0	0.0	0.0	1.4834183745543106	0.0
4	0.0	0.0	0.0	2.9647177818085244	0.0
5	0.0	0.0	0.0	3.1965328044390953	0.0
6	0.0	0.0	0.0	4.3563142400253145	0.0
7	0.0	0.0	0.0	5.2219830143581225	0.0
8	0.0	0.0	0.0	5.839732614579625	0.0
9	0.0	0.0	0.0	6.965751837850972	0.0
10	0.0	0.0	0.0	8.620524034739763	0.0
11	0.0	0.0	0.0	10.277556463415323	0.0
12	0.0	0.0	0.0	11.069908969164787	0.0
13	0.0	0.0	0.0	11.414311787673826	0.0
14	0.0	0.0	0.0	11.709554564820623	0.0
15	0.0	0.0	0.0	11.98530284280653	0.0
16	0.0	0.0	0.0	12.488063150876123	0.0
17	0.0	0.0	0.0	13.190288914128145	0.0
18	0.0	0.0	0.0	14.032366519186542	0.0
19	0.0	0.0	0.0	14.40855384719703	0.0
20	0.0	0.0	0.0	14.800421533228233	0.0
21	0.0	0.0	0.0	15.2061331389534	0.0
22	0.0	0.0	0.0	15.66877433280783	0.0
23	0.0	0.0	0.0	16.113757465828122	0.0
24	0.0	0.0	0.0	16.41874749255536	0.0
25	0.0	0.0	0.0	16.675707593813744	0.0
26	0.0	0.0	0.0	16.942838738112595	0.0
27	0.0	0.0	0.0	17.232007142332446	0.0
28	0.0	0.0	0.0	17.510863239025163	0.0
29	1.4126448667310833E-4	0.0	0.0	17.81571200126573	0.0
30	1.4126448667310833E-4	0.0	0.0	18.206308306916874	0.0
31	1.4126448667310833E-4	0.0	0.0	18.52104558322456	0.0
32	1.4126448667310833E-4	0.0	0.0	18.795663745317082	0.0
33	2.8252897334621666E-4	0.0	0.0	19.083419504670204	0.0
34	2.8252897334621666E-4	0.0	0.0	19.37188158645669	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	60	0.0	40.000004	16
TCGTTCG	30	4.86486E-6	40.000004	35
ACGTTCA	30	4.86486E-6	40.000004	19
ACTTGCG	30	4.86486E-6	40.000004	1
GGGTCGC	55	5.456968E-12	40.000004	7
CTATACG	55	5.456968E-12	40.000004	1
ATTGTCG	30	4.86486E-6	40.000004	10
CGCGAAG	30	4.86486E-6	40.000004	1
CGTTCGT	30	4.86486E-6	40.000004	36
CATTACT	60	0.0	40.000004	40
CTGCGCG	30	4.86486E-6	40.000004	1
TCGTGTG	30	4.86486E-6	40.000004	21
CGCTAAG	30	4.86486E-6	40.000004	1
CTTTGCG	60	0.0	40.000004	1
GCGATAG	30	4.86486E-6	40.000004	1
ACGTCAC	30	4.86486E-6	40.000004	20
TTACGAG	30	4.86486E-6	40.000004	1
GAACCGA	30	4.86486E-6	40.000004	9
TCGAGCG	30	4.86486E-6	40.000004	1
CGGTAAC	30	4.86486E-6	40.000004	13
>>END_MODULE