##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1006407
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1244407083814	31.0	31.0	34.0	30.0	34.0
2	31.29997804069328	31.0	31.0	34.0	30.0	34.0
3	31.42846383222692	31.0	31.0	34.0	30.0	34.0
4	35.32219171766492	37.0	35.0	37.0	33.0	37.0
5	34.88856297700632	35.0	35.0	37.0	32.0	37.0
6	34.85292431392071	35.0	35.0	37.0	32.0	37.0
7	34.05972136521308	35.0	35.0	37.0	30.0	37.0
8	34.40795523083603	37.0	35.0	37.0	31.0	37.0
9	35.651829726939496	39.0	35.0	39.0	31.0	39.0
10	35.362442828795906	37.0	35.0	39.0	30.0	39.0
11	35.76196508967048	37.0	35.0	39.0	30.0	39.0
12	35.45854510153447	37.0	35.0	39.0	30.0	39.0
13	35.47804516463022	37.0	35.0	39.0	30.0	39.0
14	36.48285733306704	38.0	35.0	40.0	31.0	41.0
15	36.61737646896335	38.0	35.0	40.0	31.0	41.0
16	36.5095950246769	38.0	35.0	40.0	31.0	41.0
17	36.40639125125322	38.0	35.0	40.0	31.0	41.0
18	36.15955870736193	38.0	35.0	40.0	30.0	41.0
19	36.15245323214167	38.0	34.0	40.0	30.0	41.0
20	36.18670279519121	38.0	34.0	40.0	30.0	41.0
21	36.330448814445845	38.0	35.0	40.0	30.0	41.0
22	36.28940577718557	38.0	34.0	40.0	30.0	41.0
23	36.24233038919642	38.0	35.0	40.0	30.0	41.0
24	35.82039572459254	38.0	34.0	40.0	29.0	41.0
25	35.8621969044333	38.0	34.0	40.0	29.0	41.0
26	35.71284877788012	38.0	34.0	40.0	29.0	41.0
27	35.5722486031993	38.0	34.0	40.0	28.0	41.0
28	35.51992980970919	38.0	34.0	40.0	28.0	41.0
29	35.27228646064664	37.0	34.0	40.0	27.0	41.0
30	35.33432100531892	38.0	34.0	40.0	27.0	41.0
31	35.18070522164492	37.0	34.0	40.0	27.0	41.0
32	35.075837111625816	37.0	34.0	40.0	27.0	41.0
33	35.03752060548069	37.0	34.0	40.0	27.0	41.0
34	34.860282172123206	37.0	33.0	40.0	26.0	41.0
35	34.71210951434161	37.0	33.0	40.0	25.0	41.0
36	34.63900887016883	37.0	33.0	40.0	25.0	41.0
37	34.526912074339705	37.0	33.0	40.0	24.0	41.0
38	34.40515616445434	37.0	33.0	40.0	24.0	41.0
39	34.19793483153436	37.0	33.0	40.0	23.0	41.0
40	33.94064429202102	36.0	33.0	40.0	23.0	41.0
41	33.927750899983806	36.0	33.0	40.0	23.0	41.0
42	33.940441590728206	36.0	33.0	40.0	23.0	41.0
43	33.948453259963415	36.0	33.0	40.0	23.0	41.0
44	33.87856801472963	36.0	33.0	40.0	23.0	41.0
45	33.644474849638364	36.0	32.0	39.0	23.0	40.0
46	33.44727133257221	35.0	32.0	39.0	22.0	40.0
47	33.337386365555886	35.0	32.0	39.0	22.0	40.0
48	33.19845251473807	35.0	32.0	39.0	22.0	40.0
49	33.02934995483935	35.0	31.0	39.0	20.0	40.0
50	32.870198637330624	35.0	31.0	39.0	20.0	40.0
51	30.56983208582611	34.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	23.0
11	32.0
12	35.0
13	44.0
14	93.0
15	170.0
16	336.0
17	651.0
18	1214.0
19	2243.0
20	3270.0
21	4824.0
22	6504.0
23	8252.0
24	9920.0
25	12056.0
26	14300.0
27	17481.0
28	21362.0
29	27023.0
30	34000.0
31	42492.0
32	53876.0
33	70654.0
34	99656.0
35	102517.0
36	108635.0
37	133745.0
38	151432.0
39	79552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.10370257758541	36.709303492523404	19.408748150599113	24.778245779292078
2	22.011671222477585	36.371964821389355	19.324686732107388	22.29167722402567
3	19.31494912098187	29.656689589798162	25.97984711950533	25.048514169714636
4	24.435442122322282	31.20238631090603	17.885706279864905	26.476465286906787
5	17.25087365250838	38.184849668176	17.52014840914262	27.044128270173
6	19.840482031623388	35.32785443662455	24.012551582014037	20.81911194973803
7	76.02997594412598	14.032990629039743	5.752344727331984	4.184688699502289
8	78.94996755785681	8.048334321998952	3.7281139737700553	9.27358414637418
9	76.1346055820359	10.662783545821918	7.1941073541817575	6.008503517960428
10	40.74097258862468	31.16313777626745	12.950029163151688	15.145860471956176
11	32.047571211249526	27.279122661110268	21.694105863731075	18.979200263909135
12	29.40003398227556	25.406619787024532	28.024049912212455	17.16929631848745
13	24.522881895694287	31.933601415729424	22.04446113749209	21.499055551084204
14	18.170680450354578	31.42376791894333	30.388600238273384	20.01695139242871
15	17.708143921892436	30.7692613425781	28.045909855555458	23.476684879974005
16	20.648405664904953	27.195359332755036	31.398231530583555	20.758003471756457
17	19.586012418435086	27.67955707780252	26.33805210019406	26.396378403568338
18	21.147408553398375	30.063383899356822	24.82156821246275	23.96763933478205
19	22.39372341408595	30.920293678402476	26.00975549653371	20.676227410977866
20	25.214451012363785	32.163329547588596	22.89481293353484	19.727406506512775
21	22.292174040919825	28.748408943896454	24.465052409214163	24.494364605969555
22	22.73672579781341	30.08693302013996	24.086477935864913	23.089863246181714
23	23.350394025478757	29.682225978157945	21.214081380594532	25.75329861576877
24	20.117407768427682	33.427728543223566	25.187722263458024	21.267141424890728
25	20.969150651774083	27.74165918957241	24.8997671916034	26.38942296705011
26	24.291663313152632	31.80413093311155	20.774597156021372	23.129608597714444
27	19.802227130773137	29.470085164351996	26.346597350773592	24.38109035410127
28	22.31562379832414	29.810504100229828	23.604664911909396	24.26920718953664
29	23.54057553256287	30.04380931372695	24.485719992011184	21.929895161698994
30	23.020706334514767	26.538368671919017	26.26462256323734	24.17630243032888
31	26.017009023188432	29.677853989489343	20.11164469245544	24.19349229486679
32	23.624338860918098	26.75478211101473	23.011068086768077	26.609810941299095
33	24.018513384743944	26.952415871511228	24.07852886555837	24.95054187818646
34	22.403262298453807	30.091801825702724	23.262159345076096	24.242776530767372
35	21.934167786988763	28.67885457871418	26.060927636632098	23.32604999766496
36	24.70978441127695	28.47843864361039	24.192399297699637	22.619377647413025
37	21.730671587141188	29.75257525037087	23.602578280953928	24.914174881534013
38	22.593344442159086	27.480333503244715	25.85365562838891	24.07266642620729
39	21.46566945579671	28.47466283521478	24.757876286631554	25.301791422356956
40	23.04544781584389	30.035164699768586	24.75877055704104	22.160616927346492
41	19.794476787224255	26.734015164838876	29.109396099192473	24.362111948744396
42	24.660500175376363	28.40282311231937	21.965964068214948	24.970712644089318
43	23.79752923022197	26.437514842404713	25.956993542374008	23.80796238499931
44	22.33102512204307	26.202321724709783	25.542151435751144	25.924501717496
45	25.14628773448515	29.148346543694547	23.269015418215496	22.436350303604804
46	20.725611010257282	27.08059463020428	29.996015528508845	22.197778831029595
47	23.98969800488272	28.55494844531089	24.043652319588396	23.411701230217993
48	22.98851259977325	24.22032040715138	29.553947856086054	23.23721913698931
49	23.053396886150434	26.065597715437193	26.74047378446295	24.140531613949427
50	22.474108387560896	26.21831922870171	24.47498874709735	26.832583636640045
51	20.987334150100306	26.41615171595587	28.385037067508474	24.21147706643535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11550.0
1	9211.5
2	6873.0
3	4736.0
4	2599.0
5	2377.5
6	2156.0
7	2270.5
8	2385.0
9	2527.5
10	2670.0
11	2826.5
12	2983.0
13	3119.0
14	3255.0
15	3434.5
16	3614.0
17	3622.5
18	3631.0
19	3686.0
20	3741.0
21	3702.0
22	3663.0
23	3980.0
24	4297.0
25	4998.5
26	7241.5
27	8783.0
28	9562.5
29	10342.0
30	12527.5
31	14713.0
32	16446.0
33	18179.0
34	18890.0
35	19601.0
36	24205.5
37	28810.0
38	31212.0
39	33614.0
40	35546.0
41	37478.0
42	45473.5
43	53469.0
44	77455.5
45	101442.0
46	96023.5
47	90605.0
48	81962.5
49	73320.0
50	69444.0
51	65568.0
52	63043.5
53	60519.0
54	55553.5
55	50588.0
56	49063.0
57	47538.0
58	45257.5
59	42977.0
60	42252.5
61	41528.0
62	40387.5
63	39247.0
64	34731.5
65	30216.0
66	26368.5
67	22521.0
68	19776.5
69	17032.0
70	14910.0
71	12788.0
72	11104.0
73	9420.0
74	8252.5
75	5661.5
76	4238.0
77	3391.0
78	2544.0
79	1803.5
80	1063.0
81	978.5
82	894.0
83	728.5
84	563.0
85	410.5
86	258.0
87	199.0
88	140.0
89	124.5
90	109.0
91	80.5
92	52.0
93	37.0
94	22.0
95	17.0
96	12.0
97	11.0
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1006407.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.60597107070288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95221041130016	18.873495660079012
2	8.595426357205424	4.058067718570966
3	3.1508328254855993	2.231354055811022
4	1.5084771295564314	1.4243626992450416
5	0.8885167400943474	1.0487150231251197
6	0.5679461135813693	0.804415171615196
7	0.3961577844251136	0.6546182439001086
8	0.29455272354931683	0.5562562456721533
9	0.20958867254689348	0.44527897268000877
>10	2.5088463270802075	14.298965459617271
>50	1.128312777121032	19.30692216081205
>100	0.7901193695798077	28.041230328592825
>500	0.003433435609255003	0.5324957515403292
>1k	0.003862615060411878	2.0945238141827947
>5k	4.291794511568754E-4	0.9856642250258494
>10k+	0.001287538353470626	4.64363446953025
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGT	17164	1.7054730342694355	No Hit
AATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT	14497	1.4404709029249598	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCC	14174	1.4083765315622805	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9729	0.9667063126548205	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	3860	0.38354264229084256	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTT	3618	0.35949670461354105	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCT	2928	0.29093597321958214	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2300	0.22853577131319636	No Hit
AACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTG	2033	0.2020057491650992	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1957	0.19445413237388054	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTAT	1422	0.14129472469885443	No Hit
AATGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	1304	0.12956984599669916	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1252	0.12440295029744428	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.98726757663649E-4	0.0	0.0	0.5429215019370891	0.0
2	1.98726757663649E-4	0.0	0.0	0.6179408529551166	0.0
3	1.98726757663649E-4	0.0	0.0	1.0237408921042879	0.0
4	1.98726757663649E-4	0.0	0.0	3.373784164855769	0.0
5	1.98726757663649E-4	0.0	0.0	3.6340168540163176	0.0
6	1.98726757663649E-4	0.0	0.0	4.475425945964207	0.0
7	1.98726757663649E-4	0.0	0.0	5.13559623492285	0.0
8	1.98726757663649E-4	0.0	0.0	5.375757521559369	0.0
9	1.98726757663649E-4	0.0	0.0	7.9387365151474505	0.0
10	1.98726757663649E-4	0.0	0.0	8.447675741524055	0.0
11	1.98726757663649E-4	0.0	0.0	10.75161440649757	0.0
12	1.98726757663649E-4	0.0	0.0	11.051393720433184	0.0
13	1.98726757663649E-4	0.0	0.0	11.242668224684447	0.0
14	1.98726757663649E-4	0.0	0.0	11.545925256879174	0.0
15	1.98726757663649E-4	0.0	0.0	11.68016518168097	0.0
16	1.98726757663649E-4	0.0	0.0	11.870446052143913	0.0
17	1.98726757663649E-4	0.0	0.0	12.084375406768832	0.0
18	1.98726757663649E-4	0.0	0.0	12.42250898493353	0.0
19	1.98726757663649E-4	0.0	0.0	12.54939601970177	0.0
20	1.98726757663649E-4	0.0	0.0	12.679263955834966	0.0
21	1.98726757663649E-4	0.0	0.0	12.824135762171766	0.0
22	1.98726757663649E-4	0.0	0.0	12.991761782261053	0.0
23	1.98726757663649E-4	0.0	0.0	13.152134275695618	0.0
24	1.98726757663649E-4	0.0	0.0	13.266799614867544	9.93633788318245E-5
25	1.98726757663649E-4	0.0	0.0	13.375602514688392	0.005365622456918523
26	1.98726757663649E-4	0.0	0.0	13.47307798932241	0.005365622456918523
27	1.98726757663649E-4	0.0	0.0	13.573335638563723	0.005365622456918523
28	1.98726757663649E-4	0.0	0.0	13.670413659682415	0.005365622456918523
29	1.98726757663649E-4	0.0	0.0	13.777030565168962	0.005365622456918523
30	1.98726757663649E-4	0.0	0.0	13.93531642764806	0.005365622456918523
31	1.98726757663649E-4	0.0	0.0	14.05246585129078	0.005365622456918523
32	2.980901364954735E-4	0.0	0.0	14.168919731281678	0.005365622456918523
33	2.980901364954735E-4	0.0	0.0	14.281001622603977	0.005365622456918523
34	2.980901364954735E-4	0.0	0.0	14.394772691366416	0.005365622456918523
35	2.980901364954735E-4	0.0	0.0	14.524342537363115	0.005365622456918523
36	2.980901364954735E-4	0.0	0.0	14.641790051142332	0.005365622456918523
37	2.980901364954735E-4	0.0	0.0	14.754170032601124	0.005365622456918523
38	2.980901364954735E-4	0.0	0.0	14.880162796959878	0.005365622456918523
39	2.980901364954735E-4	0.0	0.0	15.008043465516437	0.005365622456918523
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATT	20	7.032866E-4	45.000004	41
TCGATAT	20	7.032866E-4	45.000004	29
CGTTCCC	20	7.032866E-4	45.000004	29
TACGCTA	20	7.032866E-4	45.000004	35
TAACCGC	20	7.032866E-4	45.000004	27
CGTAGGT	20	7.032866E-4	45.000004	24
TATTACG	20	7.032866E-4	45.000004	1
CATACCG	40	6.8139343E-9	45.000004	1
TCGTAGA	20	7.032866E-4	45.000004	24
TACTCGA	20	7.032866E-4	45.000004	39
CGTGTAA	20	7.032866E-4	45.000004	41
ATGCGAT	20	7.032866E-4	45.000004	32
ACGTAAT	20	7.032866E-4	45.000004	23
CGGTCAT	20	7.032866E-4	45.000004	31
CGCGTTA	20	7.032866E-4	45.000004	39
ATCGGCC	20	7.032866E-4	45.000004	13
CGGTACG	40	6.8139343E-9	45.000004	1
CGGTAAC	20	7.032866E-4	45.000004	10
CGATCAT	20	7.032866E-4	45.000004	11
TATCGTC	20	7.032866E-4	45.000004	35
>>END_MODULE