FastQCFastQC Report
Sat 18 Jun 2016
SRR3548363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088279
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTAT147721.357372512012085No Hit
AATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGT123131.13141942461446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122151.1224143808710818No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGT122091.1218630516623034No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTT54550.5012501389809046No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31620.29055049302614494No Hit
AATGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCC29780.2736430639569449No Hit
AACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTC25300.23247714970150118No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25020.22990428006053595No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC23490.21584538523669022No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTCC18930.1739443653695422No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTCCTAAT18460.16962561990077912No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15410.14159971845455072No Hit
AATGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG13970.1283678174438724No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT11480.10548765527957445No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT200.001255739440.019
TCTGCGA257.794381E-540.018
CTTAACG700.040.01
AACCGGT257.794381E-540.037
TCGCAAG401.9281288E-840.01
CGTATTA200.001255739440.021
ATTAGCG451.2205419E-940.01
CGTATAG304.8672755E-640.01
CGAAATA304.8672755E-640.021
CGAAACG200.001255739440.01
CTATCCG257.794381E-540.01
GGCGTAT353.056175E-740.020
CGCATAG750.040.01
ATTACGT257.794381E-540.011
TATATCG353.056175E-740.01
TAACGTT200.001255739440.013
CGACACT257.794381E-540.011
CGACACG451.2205419E-940.01
CGTTCAT600.040.017
TCGTGCA257.794381E-540.030
Produced by FastQC (version 0.11.5)