SRR3548360_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	678656
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11027	1.6248290739343645	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	2839	0.41832681063749527	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2497	0.367933091286307	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGT	2407	0.3546715861938891	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	2375	0.3499563843832516	RNA PCR Primer, Index 23 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2199	0.32402277442474536	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	1854	0.27318700490380987	Illumina Paired End PCR Primer 2 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1239	0.1825667201056205	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCA	25	3.8891103E-5	45.000004	34
CTATGCG	35	1.2109376E-7	45.000004	1
GATCGAC	25	3.8891103E-5	45.000004	9
GGTCGAA	25	3.8891103E-5	45.000004	26
ACGTTCA	25	3.8891103E-5	45.000004	16
CCGATCG	25	3.8891103E-5	45.000004	26
ACGCAAC	25	3.8891103E-5	45.000004	28
GTAGGGT	100	0.0	45.000004	4
TCGATTG	25	3.8891103E-5	45.000004	15
CGCGACA	35	1.2109376E-7	45.000004	22
ATGTCGC	35	1.2109376E-7	45.000004	13
CCGTCTA	25	3.8891103E-5	45.000004	29
AGTATAC	25	3.8891103E-5	45.000004	45
ATCCGCT	25	3.8891103E-5	45.000004	28
CGCTATC	25	3.8891103E-5	45.000004	24
AGCATCG	50	2.1827873E-11	45.000004	22
TCATGTA	25	3.8891103E-5	45.000004	34
GTCCGTT	25	3.8891103E-5	45.000004	41
ACGCTAT	25	3.8891103E-5	45.000004	23
GCGATAT	25	3.8891103E-5	45.000004	14