##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548359_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	737588
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.008630834558048	31.0	31.0	34.0	28.0	34.0
2	31.334711248013797	31.0	31.0	34.0	28.0	34.0
3	31.437101742436155	31.0	31.0	34.0	28.0	34.0
4	35.43099806395983	37.0	35.0	37.0	33.0	37.0
5	34.9967393721156	37.0	35.0	37.0	32.0	37.0
6	35.17311154736791	37.0	35.0	37.0	32.0	37.0
7	35.129524883810475	37.0	35.0	37.0	32.0	37.0
8	35.50997304728385	37.0	35.0	37.0	33.0	37.0
9	36.85072831987505	39.0	37.0	39.0	32.0	39.0
10	36.127980661290586	37.0	35.0	39.0	31.0	39.0
11	36.30872112886869	38.0	35.0	39.0	32.0	39.0
12	35.97872389464037	37.0	35.0	39.0	31.0	39.0
13	35.6132054751433	37.0	35.0	39.0	30.0	39.0
14	36.78650683037143	39.0	35.0	40.0	31.0	41.0
15	36.76436032039567	38.0	35.0	40.0	31.0	41.0
16	36.668386687418995	38.0	35.0	40.0	31.0	41.0
17	36.6389203728911	38.0	35.0	40.0	31.0	41.0
18	36.491077674799484	38.0	35.0	40.0	31.0	41.0
19	36.69133039040765	38.0	35.0	40.0	31.0	41.0
20	36.496121140799474	38.0	35.0	40.0	31.0	41.0
21	36.56019078401492	38.0	35.0	40.0	31.0	41.0
22	36.61948811531641	38.0	35.0	40.0	31.0	41.0
23	36.4570993562802	38.0	35.0	40.0	31.0	41.0
24	36.05595400142085	38.0	34.0	40.0	30.0	41.0
25	36.222799177860814	38.0	35.0	40.0	30.0	41.0
26	36.219076232259745	38.0	35.0	40.0	30.0	41.0
27	36.06221901657836	38.0	35.0	40.0	30.0	41.0
28	36.042550854948836	38.0	35.0	40.0	30.0	41.0
29	35.78370580866283	38.0	34.0	40.0	30.0	41.0
30	35.77592504216446	38.0	34.0	40.0	30.0	41.0
31	35.72900725066026	38.0	34.0	40.0	30.0	41.0
32	35.683579450858744	38.0	34.0	40.0	30.0	41.0
33	35.6421810007755	38.0	34.0	40.0	29.0	41.0
34	35.596964701161085	38.0	34.0	40.0	29.0	41.0
35	35.37782610346155	37.0	34.0	40.0	28.0	41.0
36	35.323035353069734	37.0	34.0	40.0	28.0	41.0
37	35.23046199233176	37.0	34.0	40.0	28.0	41.0
38	35.013728531375236	37.0	34.0	40.0	27.0	41.0
39	34.97615335390489	37.0	34.0	40.0	27.0	41.0
40	34.7777688899494	37.0	33.0	40.0	26.0	41.0
41	34.85775121070299	37.0	34.0	40.0	26.0	41.0
42	34.742558176109156	36.0	34.0	40.0	26.0	41.0
43	34.70611099963666	36.0	33.0	40.0	26.0	41.0
44	34.59079323416324	36.0	33.0	40.0	26.0	41.0
45	34.36774323877287	36.0	33.0	40.0	25.0	41.0
46	33.21008205122643	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	10.0
11	18.0
12	13.0
13	23.0
14	31.0
15	55.0
16	104.0
17	198.0
18	369.0
19	709.0
20	1253.0
21	2142.0
22	3134.0
23	4321.0
24	5605.0
25	6770.0
26	8191.0
27	9379.0
28	11625.0
29	14386.0
30	18548.0
31	24135.0
32	32460.0
33	45606.0
34	74409.0
35	81074.0
36	74958.0
37	98941.0
38	133552.0
39	85559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.402040163343223	32.882449280628215	20.47213349457963	26.243377061448943
2	24.57022077365684	31.423911451921672	19.712495322592016	24.293372451829477
3	20.024186944473065	32.731552031757566	21.263903425760724	25.980357598008645
4	20.510772951837613	33.81169433342191	18.4798288475409	27.197703867199575
5	17.101688205339567	37.66926793819856	16.865241842329322	28.36380201413255
6	19.532313432431113	38.20981360868127	19.538143245280562	22.719729713607055
7	82.11982841369436	10.095744507773988	3.8727582335938218	3.9116688449378247
8	84.06400321046438	8.326192942401448	3.1281691133803697	4.481634733753803
9	79.61816081606534	10.800609554385375	5.396779774074416	4.184449855474872
10	42.78201380716606	32.660780815306104	10.59304110153636	13.964164275991475
11	33.20918995428342	30.406541321171172	18.59425587184173	17.790012852703676
12	31.244136292889795	26.774025607791884	23.68219114193832	18.29964695738
13	23.24427729301453	35.74637873718119	21.66222877812546	19.347115191678824
14	19.10239862904494	34.93793282970981	25.92544889558941	20.034219645655842
15	17.191033476683458	29.51200399138815	31.44709512627646	21.849867405651935
16	19.590747137968624	29.54779633074291	29.38361253165724	21.47784399963123
17	19.941756102322707	28.462773255530188	23.898165371453985	27.697305270693125
18	23.66456612634696	27.842101552628296	24.67922471623725	23.814107604787495
19	24.23615893967906	30.781682999181115	21.956024230329128	23.026133830810696
20	26.612011041394386	29.79047923773163	21.842410668286362	21.755099052587624
21	23.737506575486584	30.85679268100891	23.502415982906445	21.90328476059806
22	24.074144373281563	29.1200507600449	22.30960915850041	24.496195708173126
23	22.564765153446096	32.42663926202704	20.565139346084806	24.443456238442057
24	21.048742658503123	29.3196201673563	27.84955828999387	21.782078884146706
25	21.841190474899268	30.49168370418174	24.35939847177557	23.307727349143427
26	23.504991946723646	32.73602607417691	20.20762268366622	23.551359295433222
27	20.086281230171856	32.08417165138261	22.978681865757036	24.850865252688493
28	20.781791460815523	32.99497822632689	25.594776487686893	20.62845382517069
29	20.27514005108543	30.44274039165496	25.206483836504933	24.075635720754676
30	23.784822963497238	31.032364951707457	23.757843131938156	21.424968952857153
31	27.500447404241935	30.994945687836573	20.35404589011752	21.150561017803977
32	26.589776406340665	30.02773906299994	21.910605920920624	21.47187860973877
33	24.99105191516131	30.438673080364648	21.21862069339523	23.351654311078814
34	23.123884878821237	29.74872150848441	24.312353237850942	22.81504037484341
35	23.32142063048748	31.11899868219114	24.06478955731384	21.49479113000754
36	24.182741584732938	31.75526174503924	23.328877367853057	20.733119302374767
37	23.57061123554071	31.879585893479828	22.754844167746764	21.7949587032327
38	24.53036112301176	30.614380928106204	24.179758889786708	20.67549905909532
39	24.25635991908762	29.063786287195565	23.8271230009165	22.852730792800315
40	25.013286550215025	29.624126205957797	22.534938203983796	22.827649039843383
41	20.01103597130105	27.80563132805848	26.322960785696083	25.860371914944384
42	25.481027348601117	29.04019588171174	22.25388699382311	23.224889775864032
43	24.1173934500019	27.709642781607073	24.275069551023066	23.897894217367963
44	23.17174357500393	29.781395575849935	23.092973313014852	23.953887536131283
45	23.931246169948537	30.95657738466461	21.887422246565833	23.22475419882102
46	23.393547617369045	29.670086823538345	25.49092447274088	21.445441086351728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16166.0
1	12671.0
2	9176.0
3	6078.0
4	2980.0
5	2601.5
6	2223.0
7	2223.0
8	2265.5
9	2308.0
10	2340.5
11	2373.0
12	2412.0
13	2451.0
14	2400.0
15	2349.0
16	2418.5
17	2488.0
18	2553.0
19	2618.0
20	2618.0
21	2453.5
22	2289.0
23	2649.5
24	3010.0
25	3556.0
26	4102.0
27	5078.5
28	6055.0
29	6763.5
30	7472.0
31	7472.0
32	8994.5
33	10517.0
34	12300.5
35	14084.0
36	16300.5
37	18517.0
38	21799.5
39	25082.0
40	29966.0
41	34850.0
42	38105.0
43	41360.0
44	41360.0
45	49027.5
46	56695.0
47	61283.0
48	65871.0
49	63023.5
50	60176.0
51	56879.5
52	53583.0
53	48865.0
54	44147.0
55	43792.5
56	43438.0
57	43438.0
58	40945.0
59	38452.0
60	37332.5
61	36213.0
62	36150.0
63	36087.0
64	32490.0
65	28893.0
66	24940.0
67	20987.0
68	17758.0
69	14529.0
70	14529.0
71	12965.5
72	11402.0
73	8763.5
74	6125.0
75	4918.0
76	3711.0
77	2754.0
78	1797.0
79	1790.5
80	1784.0
81	1784.0
82	1297.5
83	811.0
84	489.5
85	168.0
86	178.5
87	189.0
88	109.0
89	29.0
90	19.5
91	10.0
92	13.5
93	17.0
94	17.0
95	10.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	737588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.380815528035676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41194596344891	15.777185903473182
2	9.354324456843125	3.8129752228862444
3	3.2946196599668425	2.014411065744715
4	1.6661126327606417	1.3582693686889793
5	0.9939235652595403	1.0128486416261109
6	0.6816569787614565	0.8335635082521227
7	0.47203426802964377	0.6734310337736461
8	0.34628768513406216	0.5646100344278258
9	0.2698162974808856	0.4949168566873973
>10	3.0675702331355525	14.799694489579613
>50	1.3125520548932823	19.48790107599035
>100	1.1197856133791235	34.36594732368307
>500	0.004015967531544974	0.4571249527897889
>1k	0.004685295453469135	2.447685176934402
>5k	0.0	0.0
>10k+	6.693279219241623E-4	1.8994353454625543
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13924	1.887774746877661	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	3401	0.4610975232785783	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3274	0.44387923881624974	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2745	0.3721589830637158	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTAT	2488	0.3373156830100273	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGT	2436	0.3302656767734833	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGT	2037	0.2761704366123093	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1562	0.21177134118234028	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.355770430104611E-4	0.0	0.0	0.5288860447838089	0.0
2	1.355770430104611E-4	0.0	0.0	0.640059220052387	0.0
3	1.355770430104611E-4	0.0	0.0	0.8168516841380282	0.0
4	1.355770430104611E-4	0.0	0.0	1.6398043352115272	0.0
5	1.355770430104611E-4	0.0	0.0	1.7866342727918567	0.0
6	1.355770430104611E-4	0.0	0.0	2.421270411123825	0.0
7	1.355770430104611E-4	0.0	0.0	2.9229054702625312	0.0
8	1.355770430104611E-4	0.0	0.0	3.30170772843376	0.0
9	1.355770430104611E-4	0.0	0.0	4.122762300905112	0.0
10	1.355770430104611E-4	0.0	0.0	4.920904353107697	0.0
11	1.355770430104611E-4	0.0	0.0	6.007283198750522	0.0
12	1.355770430104611E-4	0.0	0.0	6.38893257482497	0.0
13	1.355770430104611E-4	0.0	0.0	6.582265438157887	0.0
14	1.355770430104611E-4	0.0	0.0	6.784681963372506	0.0
15	1.355770430104611E-4	0.0	0.0	6.9218859308990925	0.0
16	1.355770430104611E-4	0.0	0.0	7.197785213425381	0.0
17	1.355770430104611E-4	0.0	0.0	7.502426829069887	0.0
18	1.355770430104611E-4	0.0	0.0	7.993758032939798	0.0
19	1.355770430104611E-4	0.0	0.0	8.15889087132654	0.0
20	1.355770430104611E-4	0.0	0.0	8.37039105842286	0.0
21	1.355770430104611E-4	0.0	0.0	8.587449904282607	0.0
22	1.355770430104611E-4	0.0	0.0	8.792442393314424	0.0
23	1.355770430104611E-4	0.0	0.0	9.029837795625742	0.0
24	1.355770430104611E-4	0.0	0.0	9.183175431270573	0.0
25	1.355770430104611E-4	0.0	0.0	9.32892075250682	0.0
26	1.355770430104611E-4	0.0	0.0	9.468971837936627	0.0
27	1.355770430104611E-4	0.0	0.0	9.621631588366405	0.0
28	1.355770430104611E-4	0.0	0.0	9.774562492882206	0.0
29	1.355770430104611E-4	0.0	0.0	9.928578013742088	0.0
30	1.355770430104611E-4	0.0	0.0	10.17288784524694	0.0
31	2.711540860209222E-4	0.0	0.0	10.321615861429416	0.0
32	2.711540860209222E-4	0.0	0.0	10.480918886966707	0.0
33	2.711540860209222E-4	0.0	0.0	10.638188256858843	0.0
34	2.711540860209222E-4	0.0	0.0	10.787865312342392	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	50	7.8216544E-11	40.000004	1
CGGAACC	25	7.791932E-5	40.000004	34
AGCCGAT	30	4.865138E-6	40.000004	17
TCCGCAA	30	4.865138E-6	40.000004	12
GTCGAGC	25	7.791932E-5	40.000004	30
GGTCGAA	25	7.791932E-5	40.000004	26
CTATCTA	25	7.791932E-5	40.000004	38
TATGGTA	25	7.791932E-5	40.000004	27
ACCGCAA	25	7.791932E-5	40.000004	18
ATCAGTC	25	7.791932E-5	40.000004	36
CGTTGTC	25	7.791932E-5	40.000004	36
TAGCGAG	25	7.791932E-5	40.000004	1
GCGTTGT	25	7.791932E-5	40.000004	35
TAGCCCG	25	7.791932E-5	40.000004	15
ACTAGGT	25	7.791932E-5	40.000004	25
AAGCCGC	30	4.865138E-6	40.000004	13
AATTGCG	25	7.791932E-5	40.000004	1
TGCGAAT	25	7.791932E-5	40.000004	11
CCGAGTG	25	7.791932E-5	40.000004	13
GATATGC	30	4.865138E-6	40.000004	34
>>END_MODULE