FastQCFastQC Report
Sat 18 Jun 2016
SRR3548358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711791
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170282.3922752605750843No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38830.5455253016686078No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33590.4719081865322826No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT20000.28098135548215697No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19110.268477685163201No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC9310.1307968209769441No Hit
AATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT9240.1298133862327565No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT8840.1241937591231134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG406.8084773E-945.0000041
AGACTCG207.031473E-445.0000041
AAATCGA207.031473E-445.00000417
CGAGTTA406.8084773E-945.00000419
TTGGACG207.031473E-445.00000417
CGAGTCG207.031473E-445.00000428
CCGATAC207.031473E-445.00000414
CTATCCG207.031473E-445.0000041
TAGCGCG207.031473E-445.0000041
CGACCCA207.031473E-445.00000439
CCCACGT207.031473E-445.00000434
GCCGATA207.031473E-445.00000413
CCCCGTA207.031473E-445.00000424
TACGCCC207.031473E-445.00000415
GTTCGGC207.031473E-445.00000436
GTTCGAT207.031473E-445.00000418
CTACGTC207.031473E-445.0000042
TACGACG207.031473E-445.0000041
TGTTCGG207.031473E-445.0000042
AGCGCGT207.031473E-445.00000421