##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	711791
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.095282182550775	31.0	31.0	33.0	28.0	34.0
2	31.23987237826834	31.0	31.0	34.0	28.0	34.0
3	31.468249809283904	31.0	31.0	34.0	30.0	34.0
4	35.41933797982835	37.0	35.0	37.0	33.0	37.0
5	35.016361544329726	35.0	35.0	37.0	32.0	37.0
6	34.98396720385619	36.0	35.0	37.0	32.0	37.0
7	34.17120615461561	36.0	35.0	37.0	31.0	37.0
8	34.52032689370897	37.0	35.0	37.0	32.0	37.0
9	35.59829219532138	39.0	35.0	39.0	30.0	39.0
10	35.374260140968346	37.0	35.0	39.0	30.0	39.0
11	35.77255402217786	37.0	35.0	39.0	30.0	39.0
12	35.65685432943097	37.0	35.0	39.0	30.0	39.0
13	35.42424953392218	37.0	35.0	39.0	30.0	39.0
14	36.48038960874751	38.0	35.0	40.0	31.0	41.0
15	36.60300706246637	38.0	35.0	40.0	31.0	41.0
16	36.570144888035955	38.0	35.0	40.0	31.0	41.0
17	36.52062894866611	38.0	35.0	40.0	31.0	41.0
18	36.33827204895819	38.0	35.0	40.0	31.0	41.0
19	36.28020303712747	38.0	34.0	40.0	30.0	41.0
20	36.16130156183486	38.0	34.0	40.0	30.0	41.0
21	36.11425263876615	38.0	34.0	40.0	30.0	41.0
22	36.1292696873099	38.0	34.0	40.0	30.0	41.0
23	35.98484105587174	38.0	34.0	40.0	30.0	41.0
24	35.640350889516725	38.0	34.0	40.0	29.0	41.0
25	35.68845630248205	38.0	34.0	40.0	29.0	41.0
26	35.55783088013195	38.0	34.0	40.0	29.0	41.0
27	35.355802475726726	37.0	34.0	40.0	27.0	41.0
28	35.30941666865695	37.0	34.0	40.0	27.0	41.0
29	35.062043493104014	37.0	34.0	40.0	27.0	41.0
30	35.056557332138226	37.0	34.0	40.0	27.0	41.0
31	34.84544058578993	37.0	33.0	40.0	25.0	41.0
32	34.779273691294215	37.0	33.0	40.0	25.0	41.0
33	34.638445835926554	37.0	33.0	40.0	25.0	41.0
34	34.527577617587184	37.0	33.0	40.0	24.0	41.0
35	34.36994848206847	37.0	33.0	40.0	24.0	41.0
36	34.170871786802586	37.0	33.0	40.0	23.0	41.0
37	33.96520186403031	36.0	33.0	40.0	23.0	41.0
38	33.8785668826945	36.0	33.0	40.0	23.0	41.0
39	33.768476982709814	36.0	33.0	40.0	22.0	41.0
40	33.54635700648083	36.0	32.0	40.0	21.0	41.0
41	33.52832081327244	36.0	33.0	40.0	21.0	41.0
42	33.42124022360496	36.0	32.0	40.0	21.0	41.0
43	33.30350763075116	36.0	32.0	39.0	20.0	41.0
44	33.16280059736636	35.0	32.0	39.0	20.0	41.0
45	33.01788165346289	35.0	32.0	39.0	20.0	40.0
46	32.888496763797235	35.0	31.0	39.0	19.0	40.0
47	32.79713567606221	35.0	31.0	39.0	18.0	40.0
48	32.62578762586209	35.0	31.0	39.0	18.0	40.0
49	32.428965805973945	35.0	31.0	39.0	17.0	40.0
50	32.2541982126776	35.0	31.0	39.0	15.0	40.0
51	30.394257584038012	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	3.0
10	10.0
11	9.0
12	16.0
13	35.0
14	58.0
15	122.0
16	239.0
17	466.0
18	1015.0
19	1941.0
20	3116.0
21	4669.0
22	6115.0
23	7864.0
24	9224.0
25	10376.0
26	11635.0
27	13265.0
28	15689.0
29	18950.0
30	22995.0
31	28539.0
32	36324.0
33	48621.0
34	72137.0
35	74478.0
36	72397.0
37	92149.0
38	105634.0
39	53695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.5952435476144	34.139796653793034	18.794561886845997	25.470397911746566
2	24.34562954575149	35.24897055455885	19.14663152526514	21.258768374424513
3	21.591028827282166	35.35981769929656	18.214054406419862	24.83509906700141
4	19.893620458814457	36.2340911868793	17.841754110406004	26.030534243900245
5	17.178778602145854	38.460165975686685	17.044750495580864	27.3163049265866
6	19.211116746348296	42.652126818124984	17.95414665259887	20.182609782927855
7	77.30822671261647	15.451164737963813	3.7075489855870614	3.533059563832642
8	79.21791649515096	13.505087869894394	3.736490065201724	3.540505569752919
9	72.19605193097412	17.799747397761422	5.996844579377935	4.007356091886523
10	37.76291074205771	37.525621987353034	11.398289666489179	13.31317760410008
11	30.16152213219892	35.83720502226075	20.43085681049634	13.570416035043994
12	28.863247779193614	33.99368634894231	22.508713934286888	14.634351937577181
13	22.809223493975058	41.04378953934511	20.511076987486497	15.635909979193329
14	18.257466025841858	41.51204496825613	24.10328312664813	16.127205879253882
15	16.903557364451082	36.593606831218715	31.235854344884945	15.266981459445258
16	18.162494327688886	35.38496553061222	29.727405937978986	16.725134203719914
17	18.08129071595454	33.049027031811306	24.66046915456925	24.209213097664907
18	20.649460305061456	34.70204034611283	24.65119676983834	19.997302578987373
19	22.676459803509736	36.87781947228891	22.76103519150987	17.68468553269148
20	25.073090275094795	35.114801957316125	21.37565661830509	18.43645114928399
21	20.81004114971951	36.054965572759414	24.104969014781023	19.030024262740046
22	22.032872008777858	33.46825121419068	23.055222670699685	21.443654106331774
23	19.435339868023057	38.23214960571291	20.66547624232394	21.66703428394009
24	18.244540883489677	35.102438777674905	26.34860513830605	20.30441520052937
25	19.33025284107273	36.52364247370365	24.322729565279698	19.82337511994392
26	20.146644169426136	39.76209308631326	21.719999269448476	18.37126347481213
27	17.283022685029735	39.73554034822019	23.805583380514786	19.175853586235288
28	19.836440752973836	37.849733980901696	24.991043719294005	17.32278154683046
29	20.066985955146947	36.79731831394328	24.982052315918576	18.1536434149912
30	21.132045783102065	36.394110068826386	23.93623971081399	18.537604437257567
31	22.259061999940993	38.63001920507565	20.16785826176504	18.94306053321832
32	24.999754141313954	35.939622726333994	21.65200178142179	17.408621350930257
33	24.428378554940988	36.16890351240743	21.8038722040599	17.59884572859168
34	21.649332458544713	36.32920337571001	23.407011327763346	18.614452837981936
35	21.906430398810887	36.68633067852783	23.487933958142207	17.91930496451908
36	24.235203873047002	35.71933334363598	23.33339421262702	16.712068570689993
37	20.952498696948965	38.22835635741391	22.640634680685764	18.17851026495137
38	22.42933670136318	36.59711909816224	22.303738035462654	18.66980616501192
39	22.613519979881737	34.35095414243788	23.696703105265453	19.338822772414936
40	23.875828719385325	34.90322299663806	22.635015053576122	18.5859332304005
41	20.263532413306713	32.9679639107547	24.83650397377882	21.931999702159764
42	25.842979189115905	33.2805556687286	21.933826080970398	18.942639061185098
43	23.892547110036514	33.36667645418388	23.682794528169083	19.05798190761052
44	21.64385332211281	35.17226264451222	24.1329266596515	19.050957373723467
45	22.259623962651958	37.52182873905402	21.70595020167437	18.512597096619654
46	21.393077462344987	35.14809824794076	24.789861068768783	18.668963220945475
47	22.789554799091306	34.810920621362165	23.252893054281383	19.14663152526514
48	23.06365211136415	32.43086804975056	25.503132239660236	19.002347599225054
49	22.86162651677248	30.793027728645068	25.71358727491637	20.631758479666082
50	19.784318711531895	33.99790106927455	24.872469587280534	21.34531063191302
51	20.79697551668959	33.719870018024956	24.747573374768717	20.73558109051674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19690.0
1	15437.5
2	11185.0
3	7443.0
4	3701.0
5	3345.5
6	2990.0
7	3186.5
8	3383.0
9	3729.0
10	4075.0
11	4307.0
12	4539.0
13	4645.5
14	4752.0
15	4626.0
16	4500.0
17	4519.5
18	4539.0
19	4578.5
20	4618.0
21	4353.5
22	4089.0
23	4221.0
24	4353.0
25	5756.5
26	8262.5
27	9365.0
28	10586.5
29	11808.0
30	13043.5
31	14279.0
32	15875.0
33	17471.0
34	18651.0
35	19831.0
36	22208.0
37	24585.0
38	27060.5
39	29536.0
40	33253.0
41	36970.0
42	42582.0
43	48194.0
44	52190.5
45	56187.0
46	55661.5
47	55136.0
48	56169.0
49	57202.0
50	54525.5
51	51849.0
52	48736.0
53	45623.0
54	39898.0
55	34173.0
56	29535.5
57	24898.0
58	21887.5
59	18877.0
60	19233.5
61	19590.0
62	17382.0
63	15174.0
64	13430.0
65	11686.0
66	10296.0
67	8906.0
68	7355.5
69	5805.0
70	4887.5
71	3970.0
72	3401.5
73	2833.0
74	2134.5
75	1100.5
76	765.0
77	838.5
78	912.0
79	696.0
80	480.0
81	362.0
82	244.0
83	227.5
84	211.0
85	157.0
86	103.0
87	74.0
88	45.0
89	35.5
90	26.0
91	28.5
92	31.0
93	20.5
94	10.0
95	7.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	711791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.034268430370098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.12924823862848	23.555283709144696
2	8.286718620382088	4.811976256622394
3	2.9788892004810688	2.594696060132937
4	1.4756714913905373	1.7138016878430973
5	0.8969286675634551	1.3020833848465772
6	0.5665220820287448	0.9869132520812834
7	0.3841596278923439	0.7807655629436183
8	0.3195621650498963	0.7422602944199138
9	0.23427898304915767	0.6121906993299033
>10	2.329720146021467	15.42442087437132
>50	0.8161692038236794	17.14074012468888
>100	0.5781815714927276	25.90227645327866
>500	0.0014812508236363646	0.3926542206588865
>1k	0.001975001098181819	1.5988581683127274
>5k	0.0	0.0
>10k+	4.937502745454548E-4	2.441079251325126
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17028	2.3922752605750843	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3883	0.5455253016686078	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3359	0.4719081865322826	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	2000	0.28098135548215697	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1911	0.268477685163201	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	931	0.1307968209769441	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	924	0.1298133862327565	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT	884	0.1241937591231134	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.404906777410785E-4	0.0	0.0	0.4304634365986645	0.0
2	1.404906777410785E-4	0.0	0.0	0.5310547618612766	0.0
3	1.404906777410785E-4	0.0	0.0	0.6537031235292382	0.0
4	1.404906777410785E-4	0.0	0.0	1.39254359776957	0.0
5	1.404906777410785E-4	0.0	0.0	1.517720791636871	0.0
6	1.404906777410785E-4	0.0	0.0	2.1367227177640626	0.0
7	1.404906777410785E-4	0.0	0.0	2.628018617824614	0.0
8	1.404906777410785E-4	0.0	0.0	3.024904782443161	0.0
9	1.404906777410785E-4	0.0	0.0	3.7543323812748404	0.0
10	1.404906777410785E-4	0.0	0.0	4.725544436498916	0.0
11	1.404906777410785E-4	0.0	0.0	5.705326423065197	0.0
12	1.404906777410785E-4	0.0	0.0	6.153210703703755	0.0
13	1.404906777410785E-4	0.0	0.0	6.389235042308767	0.0
14	1.404906777410785E-4	0.0	0.0	6.629474101246012	0.0
15	1.404906777410785E-4	0.0	0.0	6.777129803551885	0.0
16	1.404906777410785E-4	0.0	0.0	7.151256478376378	0.0
17	1.404906777410785E-4	0.0	0.0	7.531845724376959	0.0
18	1.404906777410785E-4	0.0	0.0	8.137220054763267	0.0
19	1.404906777410785E-4	0.0	0.0	8.33966712138816	0.0
20	1.404906777410785E-4	0.0	0.0	8.628656445501559	0.0
21	1.404906777410785E-4	0.0	0.0	8.861309007840784	0.0
22	1.404906777410785E-4	0.0	0.0	9.113770755741502	0.0
23	1.404906777410785E-4	0.0	0.0	9.379719608705365	0.0
24	5.61962710964314E-4	0.0	0.0	9.570786930433231	0.0
25	5.61962710964314E-4	0.0	0.0	9.720971464938444	0.0
26	5.61962710964314E-4	0.0	0.0	9.864833918945308	0.0
27	5.61962710964314E-4	0.0	0.0	10.03454665765653	0.0
28	5.61962710964314E-4	0.0	0.0	10.19442504892588	0.0
29	5.61962710964314E-4	0.0	0.0	10.372848209657048	0.0
30	5.61962710964314E-4	0.0	0.0	10.614211194016221	0.0
31	5.61962710964314E-4	0.0	0.0	10.799518397956703	0.0
32	5.61962710964314E-4	0.0	0.0	10.979486956143026	0.0
33	5.61962710964314E-4	0.0	0.0	11.150464110953918	0.0
34	5.61962710964314E-4	0.0	0.0	11.32411058864189	0.0
35	5.61962710964314E-4	0.0	0.0	11.527822071366455	0.0
36	5.61962710964314E-4	0.0	0.0	11.69922069821057	0.0
37	5.61962710964314E-4	0.0	0.0	11.871321778443392	0.0
38	5.61962710964314E-4	0.0	0.0	12.047356597652962	0.0
39	5.61962710964314E-4	0.0	0.0	12.23982882615824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	40	6.8084773E-9	45.000004	1
AGACTCG	20	7.031473E-4	45.000004	1
AAATCGA	20	7.031473E-4	45.000004	17
CGAGTTA	40	6.8084773E-9	45.000004	19
TTGGACG	20	7.031473E-4	45.000004	17
CGAGTCG	20	7.031473E-4	45.000004	28
CCGATAC	20	7.031473E-4	45.000004	14
CTATCCG	20	7.031473E-4	45.000004	1
TAGCGCG	20	7.031473E-4	45.000004	1
CGACCCA	20	7.031473E-4	45.000004	39
CCCACGT	20	7.031473E-4	45.000004	34
GCCGATA	20	7.031473E-4	45.000004	13
CCCCGTA	20	7.031473E-4	45.000004	24
TACGCCC	20	7.031473E-4	45.000004	15
GTTCGGC	20	7.031473E-4	45.000004	36
GTTCGAT	20	7.031473E-4	45.000004	18
CTACGTC	20	7.031473E-4	45.000004	2
TACGACG	20	7.031473E-4	45.000004	1
TGTTCGG	20	7.031473E-4	45.000004	2
AGCGCGT	20	7.031473E-4	45.000004	21
>>END_MODULE