FastQCFastQC Report
Sat 18 Jun 2016
SRR3548357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778923
Sequences flagged as poor quality0
Sequence length46
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220322.828520919269299No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54200.6958325790867647No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41380.5312463491256517No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT39270.5041576638512407No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25200.32352363455694594No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTAT9380.12042268619619655No Hit
AATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT9180.11785503830288745No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGT8960.11503062562024743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT401.9268555E-840.00000429
AGCGTTC257.7923345E-540.00000433
TTCTAAC401.9268555E-840.00000433
AGCGTAA200.001255519540.00000416
TTGGCGC200.001255519540.00000425
GCCCAAT200.001255519540.00000438
ACCGGAT200.001255519540.00000427
GTCGATG257.7923345E-540.0000041
TCCGCCG257.7923345E-540.00000430
CTCGTAG257.7923345E-540.00000421
CGTATCA257.7923345E-540.00000431
CTACTCG200.001255519540.0000041
CTGTACG200.001255519540.00000421
GTCGAGA257.7923345E-540.00000412
TCCGATC257.7923345E-540.00000431
TAGACCG200.001255519540.00000424
AGCCCGT507.8216544E-1140.00000415
TCACACG507.8216544E-1140.0000041
GTTTACG257.7923345E-540.0000041
TTTAACG507.8216544E-1140.0000041