##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	778923
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.960510859224854	31.0	31.0	34.0	28.0	34.0
2	31.248534194008908	31.0	31.0	34.0	28.0	34.0
3	31.442227280488574	31.0	31.0	34.0	28.0	34.0
4	35.41253885172218	37.0	35.0	37.0	33.0	37.0
5	34.979836261093844	37.0	35.0	37.0	32.0	37.0
6	35.11251946598059	36.0	35.0	37.0	32.0	37.0
7	35.0089020352461	37.0	35.0	37.0	32.0	37.0
8	35.43532801059925	37.0	35.0	37.0	33.0	37.0
9	36.714115515911075	39.0	37.0	39.0	32.0	39.0
10	36.07224077347825	37.0	35.0	39.0	31.0	39.0
11	36.293939195530236	38.0	35.0	39.0	32.0	39.0
12	36.06619781416135	37.0	35.0	39.0	32.0	39.0
13	35.67645710808385	37.0	35.0	39.0	30.0	39.0
14	36.85487782489412	39.0	35.0	40.0	31.0	41.0
15	36.82586853899551	38.0	35.0	40.0	31.0	41.0
16	36.703531671294854	38.0	35.0	40.0	31.0	41.0
17	36.6828287263311	38.0	35.0	40.0	31.0	41.0
18	36.49221553349946	38.0	35.0	40.0	31.0	41.0
19	36.68845701051323	38.0	35.0	40.0	31.0	41.0
20	36.412632570870294	38.0	35.0	40.0	31.0	41.0
21	36.44795827058644	38.0	35.0	40.0	31.0	41.0
22	36.56349215519377	38.0	35.0	40.0	31.0	41.0
23	36.34170129781763	38.0	35.0	40.0	31.0	41.0
24	35.95672229475827	38.0	34.0	40.0	30.0	41.0
25	36.12230477210199	38.0	35.0	40.0	30.0	41.0
26	36.14126300032224	38.0	35.0	40.0	30.0	41.0
27	35.98924925827072	38.0	34.0	40.0	30.0	41.0
28	35.988093816718724	38.0	35.0	40.0	30.0	41.0
29	35.64694456319816	38.0	34.0	40.0	29.0	41.0
30	35.65548584391525	38.0	34.0	40.0	29.0	41.0
31	35.54190208788288	38.0	34.0	40.0	29.0	41.0
32	35.509402084673326	38.0	34.0	40.0	29.0	41.0
33	35.42446044089082	38.0	34.0	40.0	28.0	41.0
34	35.33371591286944	38.0	34.0	40.0	27.0	41.0
35	35.10324512178996	38.0	34.0	40.0	27.0	41.0
36	35.03623849854222	37.0	34.0	40.0	26.0	41.0
37	34.84957948346627	37.0	34.0	40.0	25.0	41.0
38	34.676670222859	37.0	33.0	40.0	25.0	41.0
39	34.653696193333616	37.0	33.0	40.0	24.0	41.0
40	34.454675237475335	37.0	33.0	40.0	24.0	41.0
41	34.4774066242748	37.0	33.0	40.0	24.0	41.0
42	34.35401060181815	37.0	33.0	40.0	23.0	41.0
43	34.25481337693199	37.0	33.0	40.0	23.0	41.0
44	34.09608651946341	36.0	33.0	40.0	23.0	41.0
45	33.904949526461536	36.0	33.0	40.0	23.0	41.0
46	32.911362227075074	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	16.0
11	18.0
12	13.0
13	33.0
14	28.0
15	54.0
16	93.0
17	219.0
18	427.0
19	859.0
20	1480.0
21	2500.0
22	3925.0
23	5459.0
24	7179.0
25	8612.0
26	9997.0
27	11391.0
28	12991.0
29	16292.0
30	20351.0
31	26242.0
32	34104.0
33	47531.0
34	75822.0
35	85377.0
36	77728.0
37	102756.0
38	138119.0
39	89291.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.136107163352474	33.459019697710815	18.80802081848912	26.596852320447596
2	24.58985034464254	34.45667928665606	18.624562376512184	22.328907992189215
3	21.315072221516118	35.09615199448469	18.212069742452076	25.37670604154711
4	19.393059390979598	36.248383986607145	18.022192180741868	26.336364441671385
5	17.389652122225176	38.05600810349675	17.126083065976996	27.42825670830108
6	19.17776211512563	42.338844789536324	18.213867095977395	20.269525999360656
7	78.4533259385074	13.632284577551312	4.167420913235326	3.7469685707059623
8	79.51402128323338	12.655551318936531	4.0916753003827075	3.738752097447373
9	73.0549746252197	16.075915077613576	6.652005397195872	4.217104899970857
10	37.135249568956105	37.660975475111144	11.526556540248523	13.67721841568422
11	29.554012399171675	35.6145601041438	21.129046131645875	13.702381365038649
12	28.65597754848682	33.519359423203575	23.13746031379225	14.687202714517353
13	22.985583940903016	40.393337980776025	20.883578993045525	15.737499085275438
14	18.03438850823509	41.12678660149977	24.40395263716696	16.434872253098188
15	16.597789511928653	36.185861760405075	31.45176095711643	15.764587770549848
16	17.850031389495495	35.55691640893901	29.5334712160252	17.05958098554029
17	17.783914456242787	32.845480233604604	25.114164044456256	24.256441265696353
18	20.70153275740991	34.08026210549695	24.906826477071547	20.311378660021596
19	22.782739757331598	36.639565143152794	23.061201171360967	17.516493928154645
20	24.570207838258725	34.84914426714836	21.730389268258865	18.850258626334053
21	20.746980125121482	36.27316178877758	24.11085563014573	18.86900245595521
22	21.755808982402623	33.71450066309507	23.125135603904365	21.40455475059794
23	19.133470188966047	38.136760629741325	20.70859378911651	22.021175392176122
24	17.75220400476042	34.928998116630275	26.486828608219298	20.83196927039001
25	19.12717945162744	36.75896077019166	24.279293331946803	19.834566446234096
26	20.188388325932088	39.34548087551658	21.858386515740325	18.60774428281101
27	17.04289127423378	39.36717750021504	23.867057462676026	19.72287376287515
28	19.944461776067723	37.66341474060979	25.112623455720268	17.279500027602214
29	20.081959320754425	36.70054678061888	25.00889048083058	18.20860341779611
30	21.168844673992165	36.32926489524638	24.01919060035459	18.482699830406858
31	22.334171670370498	38.46567632487422	20.295587625477744	18.90456437927754
32	25.058446085171447	35.902521815378414	21.65823836245688	17.380793736993258
33	24.23898126002185	35.893663430146496	22.010262888629555	17.857092421202093
34	21.527416702292783	36.05927671926493	23.646624891035444	18.76668168740684
35	22.00140450339764	36.504378481570065	23.554959861244306	17.939257153787988
36	23.94126248679266	35.55948405683232	23.702471232714913	16.796782223660106
37	20.781258224497158	38.36438261548317	22.657438540138113	18.196920619881553
38	22.391686983180623	36.52646025345253	22.720731060708182	18.36112170265867
39	22.87761434698937	34.12943256265382	23.627624296624955	19.36532879373186
40	23.981446176322947	34.73128922884547	22.58387542799481	18.70338916683677
41	20.20777406752657	33.11200208493009	24.84879763468276	21.831426212860578
42	25.58917890471844	33.45516822587085	22.049291136607856	18.906361732802857
43	23.545202799249733	33.18684902102005	24.22819713886995	19.039751040860263
44	21.813452677607415	34.53447901782333	24.240008319179175	19.412059985390083
45	22.195775448921136	37.04923336453025	22.259966646253865	18.49502454029474
46	21.228157340327606	34.68147685971527	25.396091783141593	18.694274016815527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25567.0
1	20154.5
2	14742.0
3	9911.0
4	5080.0
5	4706.5
6	4333.0
7	4333.0
8	4199.5
9	4066.0
10	4298.0
11	4530.0
12	4691.0
13	4852.0
14	4785.5
15	4719.0
16	4618.5
17	4518.0
18	4768.5
19	5019.0
20	5019.0
21	4955.5
22	4892.0
23	5242.0
24	5592.0
25	6290.5
26	6989.0
27	9329.5
28	11670.0
29	13706.5
30	15743.0
31	15743.0
32	16336.0
33	16929.0
34	18121.5
35	19314.0
36	23425.0
37	27536.0
38	29862.0
39	32188.0
40	38582.0
41	44976.0
42	49859.5
43	54743.0
44	54743.0
45	60323.5
46	65904.0
47	71846.5
48	77789.0
49	73291.5
50	68794.0
51	63424.0
52	58054.0
53	51355.0
54	44656.0
55	39723.0
56	34790.0
57	34790.0
58	31193.5
59	27597.0
60	25712.5
61	23828.0
62	21044.5
63	18261.0
64	16096.5
65	13932.0
66	12441.0
67	10950.0
68	9502.0
69	8054.0
70	8054.0
71	5651.5
72	3249.0
73	2653.5
74	2058.0
75	1583.0
76	1108.0
77	840.5
78	573.0
79	733.0
80	893.0
81	893.0
82	578.0
83	263.0
84	171.5
85	80.0
86	62.5
87	45.0
88	35.0
89	25.0
90	18.5
91	12.0
92	9.0
93	6.0
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	778923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.625949091896835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14391285100326	18.22598157767529
2	9.654498334037527	4.561933722955469
3	3.490498110371929	2.4739899198302786
4	1.8248809892873994	1.7245818140669773
5	1.1716362694173528	1.384050942773715
6	0.8076769875525744	1.1449281234368232
7	0.5142443076828848	0.8504656883879494
8	0.4169426447823342	0.7880532559894602
9	0.3042426231485166	0.6469218653482806
>10	2.897541001735102	15.37537961759649
>50	0.9423810620416593	16.2872471560293
>100	0.824992922795449	30.960500898234617
>500	0.0038219394173184267	0.6693583619191517
>1k	0.0016379740359936115	1.3654188207581848
>5k	5.459913453312039E-4	0.6991563541340917
>10k+	5.459913453312039E-4	2.8420318808638947
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22032	2.828520919269299	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5420	0.6958325790867647	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4138	0.5312463491256517	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT	3927	0.5041576638512407	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2520	0.32352363455694594	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTAT	938	0.12042268619619655	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT	918	0.11785503830288745	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGT	896	0.11503062562024743	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2838239466545474E-4	0.0	0.0	0.5777207759945463	0.0
2	1.2838239466545474E-4	0.0	0.0	0.7367865629850447	0.0
3	1.2838239466545474E-4	0.0	0.0	0.9163935331220159	0.0
4	1.2838239466545474E-4	0.0	0.0	1.756913070996748	0.0
5	1.2838239466545474E-4	0.0	0.0	1.8986472347074101	0.0
6	1.2838239466545474E-4	0.0	0.0	2.6661171900174985	0.0
7	1.2838239466545474E-4	0.0	0.0	3.217904722289623	0.0
8	1.2838239466545474E-4	0.0	0.0	3.6920209057891475	0.0
9	1.2838239466545474E-4	0.0	0.0	4.464369392096523	0.0
10	1.2838239466545474E-4	0.0	0.0	5.639710215258761	0.0
11	1.2838239466545474E-4	0.0	0.0	6.744312338960333	0.0
12	1.2838239466545474E-4	0.0	0.0	7.297126930389782	0.0
13	1.2838239466545474E-4	0.0	0.0	7.561209516216622	0.0
14	1.2838239466545474E-4	0.0	0.0	7.820028423862179	0.0
15	1.2838239466545474E-4	0.0	0.0	8.000534070761809	0.0
16	2.567647893309095E-4	0.0	0.0	8.413925381584573	0.0
17	2.567647893309095E-4	0.0	0.0	8.860310967836359	0.0
18	2.567647893309095E-4	0.0	0.0	9.483222346753145	0.0
19	2.567647893309095E-4	0.0	0.0	9.73189904522013	0.0
20	2.567647893309095E-4	0.0	0.0	10.043996646651852	0.0
21	2.567647893309095E-4	0.0	0.0	10.335167917753102	0.0
22	2.567647893309095E-4	0.0	0.0	10.625697276881027	0.0
23	5.13529578661819E-4	0.0	0.0	10.936511054366093	0.0
24	8.986767626581832E-4	0.0	0.0	11.15322053656138	0.0
25	8.986767626581832E-4	0.0	0.0	11.338861479247628	0.0
26	8.986767626581832E-4	0.0	0.0	11.5184684493846	0.0
27	8.986767626581832E-4	0.0	0.0	11.729400723819941	0.0
28	8.986767626581832E-4	0.0	0.0	11.920433727082138	0.0
29	8.986767626581832E-4	0.0	0.0	12.121994086706902	0.0
30	8.986767626581832E-4	0.0	0.0	12.45309228254911	0.0
31	8.986767626581832E-4	0.0	0.0	12.661200144301812	0.0
32	8.986767626581832E-4	0.0	0.0	12.859422561665273	0.0
33	8.986767626581832E-4	0.0	0.0	13.064192481156674	0.0
34	8.986767626581832E-4	0.0	0.0	13.264340634440117	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	40	1.9268555E-8	40.000004	29
AGCGTTC	25	7.7923345E-5	40.000004	33
TTCTAAC	40	1.9268555E-8	40.000004	33
AGCGTAA	20	0.0012555195	40.000004	16
TTGGCGC	20	0.0012555195	40.000004	25
GCCCAAT	20	0.0012555195	40.000004	38
ACCGGAT	20	0.0012555195	40.000004	27
GTCGATG	25	7.7923345E-5	40.000004	1
TCCGCCG	25	7.7923345E-5	40.000004	30
CTCGTAG	25	7.7923345E-5	40.000004	21
CGTATCA	25	7.7923345E-5	40.000004	31
CTACTCG	20	0.0012555195	40.000004	1
CTGTACG	20	0.0012555195	40.000004	21
GTCGAGA	25	7.7923345E-5	40.000004	12
TCCGATC	25	7.7923345E-5	40.000004	31
TAGACCG	20	0.0012555195	40.000004	24
AGCCCGT	50	7.8216544E-11	40.000004	15
TCACACG	50	7.8216544E-11	40.000004	1
GTTTACG	25	7.7923345E-5	40.000004	1
TTTAACG	50	7.8216544E-11	40.000004	1
>>END_MODULE