SRR3548356_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572874
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGT	11767	2.0540293328026755	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC	10458	1.8255323160066612	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	9764	1.7043887486602638	TruSeq Adapter, Index 9 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6915	1.2070717121042325	No Hit
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	2642	0.4611834364973799	RNA PCR Primer, Index 17 (96% over 25bp)
AATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT	2451	0.4278427717089622	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT	1846	0.3222349068032412	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1732	0.3023352430028243	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1507	0.2630595907651595	No Hit
AACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	1321	0.23059171824868996	RNA PCR Primer, Index 17 (95% over 23bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTAT	1257	0.21941997716775416	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	892	0.15570614131554233	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGG	823	0.14366160796265845	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	722	0.1260312040693067	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGG	40	6.8048394E-9	45.000004	2
GTCGAGG	30	2.1635879E-6	45.000004	2
GATCGCG	20	7.030319E-4	45.000004	1
TCCGATT	20	7.030319E-4	45.000004	41
TAGACCG	30	2.1635879E-6	45.000004	1
TCCGAAC	20	7.030319E-4	45.000004	29
CGGTTCC	20	7.030319E-4	45.000004	35
CGATTAT	20	7.030319E-4	45.000004	33
TCGATTA	20	7.030319E-4	45.000004	32
TAACGCG	20	7.030319E-4	45.000004	1
CGTTAGT	20	7.030319E-4	45.000004	17
TAGTTAC	30	2.1635879E-6	45.000004	18
AGACGTA	20	7.030319E-4	45.000004	17
ACGATCT	20	7.030319E-4	45.000004	32
TCGGAAC	20	7.030319E-4	45.000004	37
TCTAACG	20	7.030319E-4	45.000004	1
TTGCGAG	35	1.210501E-7	45.000004	1
GCGATAG	20	7.030319E-4	45.000004	1
CGTACCG	20	7.030319E-4	45.000004	1
ACGTCGA	20	7.030319E-4	45.000004	29