##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572874
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.075873577784993	31.0	31.0	33.0	28.0	34.0
2	31.25068688751802	31.0	31.0	34.0	28.0	34.0
3	31.345255675768144	31.0	31.0	34.0	28.0	34.0
4	35.225079162259064	37.0	35.0	37.0	33.0	37.0
5	34.80034003986915	35.0	35.0	37.0	32.0	37.0
6	34.759378152962086	35.0	35.0	37.0	32.0	37.0
7	34.01738427647266	35.0	35.0	37.0	30.0	37.0
8	34.315378250714815	36.0	35.0	37.0	31.0	37.0
9	35.571914592039434	38.0	35.0	39.0	30.0	39.0
10	35.29306793465928	37.0	35.0	39.0	30.0	39.0
11	35.69994798158059	37.0	35.0	39.0	30.0	39.0
12	35.37628867778953	37.0	35.0	39.0	30.0	39.0
13	35.39126055642253	37.0	35.0	39.0	30.0	39.0
14	36.360274685183825	38.0	35.0	40.0	30.0	41.0
15	36.48947237961576	38.0	35.0	40.0	31.0	41.0
16	36.34812541675831	38.0	35.0	40.0	30.0	41.0
17	36.26442812904757	38.0	35.0	40.0	30.0	41.0
18	35.948852627279294	38.0	34.0	40.0	29.0	41.0
19	35.92050782545551	38.0	34.0	40.0	29.0	41.0
20	35.98173245774812	38.0	34.0	40.0	30.0	41.0
21	36.19101233430039	38.0	34.0	40.0	30.0	41.0
22	36.13466661080796	38.0	34.0	40.0	30.0	41.0
23	36.151878074410774	38.0	34.0	40.0	30.0	41.0
24	35.767479410830305	38.0	34.0	40.0	29.0	41.0
25	35.794590084381554	38.0	34.0	40.0	29.0	41.0
26	35.64018440355122	38.0	34.0	40.0	29.0	41.0
27	35.547703334415594	38.0	34.0	40.0	29.0	41.0
28	35.37432489517765	37.0	34.0	40.0	27.0	41.0
29	35.07512821318475	37.0	34.0	40.0	27.0	41.0
30	35.22174335019568	37.0	34.0	40.0	27.0	41.0
31	34.88311216777162	37.0	33.0	40.0	26.0	41.0
32	34.94949500239145	37.0	34.0	40.0	26.0	41.0
33	34.74526684750923	37.0	33.0	40.0	25.0	41.0
34	34.50063539277398	37.0	33.0	40.0	25.0	41.0
35	34.54759336258933	37.0	33.0	40.0	25.0	41.0
36	33.92408801935504	36.0	33.0	40.0	23.0	41.0
37	34.20562985927097	37.0	33.0	40.0	24.0	41.0
38	33.81841207665211	36.0	33.0	40.0	23.0	41.0
39	33.789714666750456	36.0	33.0	40.0	22.0	41.0
40	33.574325244294556	36.0	32.0	40.0	22.0	41.0
41	33.58071757489431	36.0	32.0	40.0	22.0	41.0
42	33.67962064956692	36.0	33.0	40.0	22.0	41.0
43	33.61204208953452	36.0	33.0	40.0	22.0	41.0
44	33.50607463421276	36.0	32.0	39.0	22.0	40.0
45	33.26663454791106	35.0	32.0	39.0	21.0	40.0
46	33.0096513369432	35.0	31.0	39.0	20.0	40.0
47	32.870151202533194	35.0	31.0	39.0	20.0	40.0
48	32.73796681294665	35.0	31.0	39.0	19.0	40.0
49	32.69163899915164	35.0	31.0	39.0	19.0	40.0
50	32.44416922394802	35.0	31.0	39.0	18.0	40.0
51	30.196100713245844	34.0	26.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	18.0
11	15.0
12	24.0
13	25.0
14	39.0
15	95.0
16	218.0
17	357.0
18	720.0
19	1239.0
20	2040.0
21	3054.0
22	4321.0
23	5598.0
24	6638.0
25	8143.0
26	9462.0
27	11010.0
28	13140.0
29	16430.0
30	20488.0
31	24872.0
32	31451.0
33	40920.0
34	56732.0
35	57531.0
36	59496.0
37	73768.0
38	82949.0
39	42073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.076271571061003	39.51182982645399	16.877882396478107	24.534016206006907
2	22.09368901364	38.573927251018546	18.765208405338697	20.567175330002758
3	18.820019760017036	28.878601577310192	28.460184962138268	23.8411937005345
4	25.393018360058235	31.39241787897513	16.80858269008543	26.405981070881207
5	16.578863764108686	40.9243568393748	17.031493836340974	25.465285560175538
6	19.980309806344852	34.6020241798371	24.899192492589993	20.518473521228053
7	73.56888251168668	15.887263167817006	6.10518194227701	4.4386723782192945
8	76.62924133404553	7.98168532696544	4.091126495529558	11.297946843459469
9	74.41985497683609	11.660330194772325	7.629251807552794	6.290563020838788
10	39.51462276172422	33.13311478614844	13.08664732558992	14.265615126537423
11	32.76619291502145	26.756319888841173	22.038353983598487	18.439133212538884
12	28.874935849768015	25.240105154012927	30.021959453562214	15.86299954265685
13	25.64647723583196	33.07708152228937	21.180748297182276	20.095692944696392
14	17.240789423154133	33.09925044599685	31.73315598194367	17.926804148905344
15	16.109650638709386	30.605857483495498	29.329311506544197	23.95518037125092
16	19.96128293481638	27.748684702046173	32.94406798004448	19.345964383092966
17	19.40321955613276	28.228720451617633	26.708141755429644	25.659918236819962
18	21.339771049131222	30.436361224283175	24.710145686486033	23.51372204009957
19	22.22530608824977	31.27895488362188	27.38769781836844	19.108041209759914
20	24.993279499506	33.82139877180671	22.209944944263484	18.9753767844238
21	21.500888502532845	29.44434552798696	25.128213184749175	23.926552784731022
22	21.125587825595158	32.10548916515673	24.787998757143807	21.980924252104302
23	23.18642493811903	29.313601245649128	21.37415906464598	26.125814751585864
24	20.048911278919974	34.76890206223358	24.74924678026931	20.432939878577137
25	21.146011164758743	28.082789583747907	25.875148811082365	24.89605044041098
26	23.421206059273068	32.58500123936503	20.4840855057133	23.50970719564861
27	20.93636646103681	29.72713022409814	27.22919874178266	22.107304573082388
28	24.017672297922406	30.881310724522322	23.708354716743997	21.392662260811278
29	19.717773890942862	32.513083156156505	28.077203713207442	19.691939239693195
30	27.157280658574134	26.829634439684817	23.44058204771032	22.57250285403073
31	27.397123974905472	32.39665266707863	20.816444802871136	19.389778555144762
32	27.296927422085833	27.28278818728028	22.297573288367076	23.122711102266816
33	29.074630721589738	31.223968970489146	20.277059178807207	19.424341129113905
34	21.50193585325918	28.698282693925712	28.162737355858358	21.63704409695675
35	29.58591243449694	27.457346641669893	23.867901144056113	19.088839779777054
36	23.478286673858477	31.32992595230365	27.420340249339297	17.771447124498582
37	28.164832057311035	28.546067721697966	22.040448685051164	21.248651535939842
38	24.65201772117429	33.30732412362928	22.43442711660854	19.606231038587893
39	24.30656653993723	26.995814088263735	27.53677073841717	21.16084863338186
40	27.030551220687272	30.282051550602752	22.104337079357762	20.583060149352214
41	20.057115526276284	25.170107213802684	32.23832814894724	22.534449110973792
42	29.32774048045469	25.64368430056173	22.14378729004982	22.88478792893376
43	25.067466842621588	25.286188585971786	26.164392170005968	23.481952401400658
44	22.482605250020075	26.323764038863693	25.54209128010697	25.65153943100926
45	26.90609104270747	29.325296662093237	22.997901807378238	20.770710487821056
46	21.698837789810675	26.40929768151461	31.44949849356054	20.44236603511418
47	24.460701655163263	29.629028372731174	23.082737216211594	22.827532755893966
48	22.156355498765873	24.698973945405097	30.70291198413613	22.4417585716929
49	23.800347022207326	24.97425262797753	27.28540656409612	23.939993785719025
50	22.62626685798273	26.891079015629966	24.336590594092243	26.14606353229506
51	20.264316411636766	25.999958105970943	29.35270233943241	24.383023142959885
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8244.0
1	6586.0
2	4928.0
3	3347.0
4	1766.0
5	1605.5
6	1445.0
7	1527.5
8	1610.0
9	1772.5
10	1935.0
11	1895.5
12	1856.0
13	1834.5
14	1813.0
15	1738.5
16	1664.0
17	1679.5
18	1695.0
19	1672.5
20	1650.0
21	1750.5
22	1851.0
23	1963.5
24	2076.0
25	2920.5
26	4274.5
27	4784.0
28	5097.5
29	5411.0
30	7081.5
31	8752.0
32	10372.0
33	11992.0
34	13178.0
35	14364.0
36	15168.5
37	15973.0
38	16645.5
39	17318.0
40	19681.0
41	22044.0
42	25777.0
43	29510.0
44	46447.5
45	63385.0
46	59344.5
47	55304.0
48	50135.0
49	44966.0
50	41531.0
51	38096.0
52	35518.5
53	32941.0
54	31776.0
55	30611.0
56	27569.5
57	24528.0
58	23403.5
59	22279.0
60	22472.0
61	22665.0
62	21918.5
63	21172.0
64	17789.5
65	14407.0
66	12714.5
67	11022.0
68	9399.5
69	7777.0
70	6708.0
71	5639.0
72	4840.5
73	4042.0
74	3456.5
75	2301.0
76	1731.0
77	1537.0
78	1343.0
79	971.5
80	600.0
81	518.0
82	436.0
83	336.5
84	237.0
85	230.5
86	224.0
87	143.0
88	62.0
89	49.0
90	36.0
91	30.5
92	25.0
93	22.0
94	19.0
95	13.0
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	572874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.966402338518787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69032350659963	21.759277285042057
2	8.055187074016034	4.3443883109990455
3	2.685450286134439	2.1725079862797503
4	1.31924717799388	1.4230140074295388
5	0.7930569512276137	1.069294641208145
6	0.5019307336188091	0.8121159665299622
7	0.3916627468445569	0.7393214648693825
8	0.33583487126399475	0.7245006606247636
9	0.274359627917755	0.6658642900688843
>10	3.4292293972350514	22.47399617413419
>50	1.1920733318407806	22.554245254542295
>100	0.3217543063818036	11.55226546607988
>500	0.002637330380178718	0.5241493304091404
>1k	0.004615328165312756	2.267994863873472
>5k	0.001318665190089359	2.9654975176031386
>10k+	0.001318665190089359	3.9515667803063583
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGT	11767	2.0540293328026755	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC	10458	1.8255323160066612	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	9764	1.7043887486602638	TruSeq Adapter, Index 9 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6915	1.2070717121042325	No Hit
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	2642	0.4611834364973799	RNA PCR Primer, Index 17 (96% over 25bp)
AATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT	2451	0.4278427717089622	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT	1846	0.3222349068032412	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1732	0.3023352430028243	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1507	0.2630595907651595	No Hit
AACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	1321	0.23059171824868996	RNA PCR Primer, Index 17 (95% over 23bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTAT	1257	0.21941997716775416	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	892	0.15570614131554233	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGG	823	0.14366160796265845	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	722	0.1260312040693067	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6942189731075246	0.0
2	0.0	0.0	0.0	0.7799271742128286	0.0
3	0.0	0.0	0.0	1.241285169164598	0.0
4	0.0	0.0	0.0	4.036315140851217	0.0
5	0.0	0.0	0.0	4.298676497798818	0.0
6	0.0	0.0	0.0	5.290168518731868	0.0
7	0.0	0.0	0.0	6.057702042683033	0.0
8	0.0	0.0	0.0	6.341534089520557	0.0
9	0.0	0.0	0.0	9.562661248372242	0.0
10	0.0	0.0	0.0	10.116011548787343	0.0
11	0.0	0.0	0.0	13.104801404846441	0.0
12	0.0	0.0	0.0	13.515886564934	0.0
13	0.0	0.0	0.0	13.800416845589082	0.0
14	0.0	0.0	0.0	14.24885751491602	0.0
15	0.0	0.0	0.0	14.449425179009696	0.0
16	0.0	0.0	0.0	14.70026567796758	0.0
17	0.0	0.0	0.0	14.988636244619236	0.0
18	0.0	0.0	0.0	15.467799201918746	0.0
19	0.0	0.0	0.0	15.620014174146496	0.0
20	0.0	0.0	0.0	15.8352447484089	0.0
21	0.0	0.0	0.0	16.02551346369359	0.0
22	0.0	0.0	0.0	16.21316380216243	0.0
23	0.0	0.0	0.0	16.414778816982444	0.0
24	0.0	0.0	0.0	16.548839709953672	0.0
25	0.0	0.0	0.0	16.672077978752746	0.0
26	0.0	0.0	0.0	16.773496440753114	0.0
27	0.0	0.0	0.0	16.920649217803565	0.0
28	0.0	0.0	0.0	17.049298798688717	0.0
29	0.0	0.0	0.0	17.171315158307063	0.0
30	0.0	0.0	0.0	17.354077860052996	0.0
31	0.0	0.0	0.0	17.48534581775399	0.0
32	0.0	0.0	0.0	17.606838502009168	0.0
33	0.0	0.0	0.0	17.731298679988967	0.0
34	0.0	0.0	0.0	17.86343942996191	0.0
35	0.0	0.0	0.0	17.999944141294595	0.0
36	0.0	0.0	0.0	18.12580078690951	0.0
37	0.0	0.0	0.0	18.255497718521003	0.0
38	0.0	0.0	0.0	18.396540949667816	0.0
39	0.0	0.0	0.0	18.556960169251877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGG	40	6.8048394E-9	45.000004	2
GTCGAGG	30	2.1635879E-6	45.000004	2
GATCGCG	20	7.030319E-4	45.000004	1
TCCGATT	20	7.030319E-4	45.000004	41
TAGACCG	30	2.1635879E-6	45.000004	1
TCCGAAC	20	7.030319E-4	45.000004	29
CGGTTCC	20	7.030319E-4	45.000004	35
CGATTAT	20	7.030319E-4	45.000004	33
TCGATTA	20	7.030319E-4	45.000004	32
TAACGCG	20	7.030319E-4	45.000004	1
CGTTAGT	20	7.030319E-4	45.000004	17
TAGTTAC	30	2.1635879E-6	45.000004	18
AGACGTA	20	7.030319E-4	45.000004	17
ACGATCT	20	7.030319E-4	45.000004	32
TCGGAAC	20	7.030319E-4	45.000004	37
TCTAACG	20	7.030319E-4	45.000004	1
TTGCGAG	35	1.210501E-7	45.000004	1
GCGATAG	20	7.030319E-4	45.000004	1
CGTACCG	20	7.030319E-4	45.000004	1
ACGTCGA	20	7.030319E-4	45.000004	29
>>END_MODULE