FastQCFastQC Report
Sat 18 Jun 2016
SRR3548355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609422
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTAT112401.8443705675213564No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGT89131.4625333512738299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87671.4385762246850293No Hit
AATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGT78741.2920439367138044No Hit
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT33910.5564288785111138No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22860.3751095300136851No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC20050.32900026582565123No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18480.3032381502472835No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAG17190.28207055209690496No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC16320.2677947300885101No Hit
AACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTC15110.2479398512032713No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAGATGT14610.23973535579614783No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10560.1732789429984477No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCGTGAGATGTCGTAT7930.13012329715697826No Hit
AATGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG7850.1288105778918385No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA200.001255304840.021
TCGCCTA200.001255304840.031
CTTCGAC200.001255304840.022
AACGTAT200.001255304840.010
AAGTACG200.001255304840.01
GGTACGT200.001255304840.01
GCCCACG200.001255304840.01
AAATCCG401.925764E-840.01
AGATCCG200.001255304840.035
ACACGAA257.7903394E-540.027
CGTATTA200.001255304840.031
ATGATCC257.7903394E-540.02
GTCGAGG507.8216544E-1140.02
CGAAATC200.001255304840.016
CGAGTAT401.925764E-840.019
CTATCCG200.001255304840.01
TAGACGG451.2187229E-940.02
TAGACCG507.8216544E-1140.01
GCGCGAA200.001255304840.019
TTTCGCG257.7903394E-540.01
Produced by FastQC (version 0.11.5)