##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185540
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14850785296152	31.0	31.0	34.0	28.0	34.0
2	31.323906405519846	31.0	31.0	34.0	28.0	34.0
3	31.527257620999713	31.0	31.0	34.0	30.0	34.0
4	35.46061035477504	37.0	35.0	37.0	33.0	37.0
5	35.058620544224574	37.0	35.0	37.0	32.0	37.0
6	35.05114040859018	36.0	35.0	37.0	32.0	37.0
7	34.24786004689846	36.0	35.0	37.0	31.0	37.0
8	34.57100224370329	37.0	35.0	37.0	32.0	37.0
9	35.68024781955902	39.0	35.0	39.0	31.0	39.0
10	35.43950942186683	37.0	35.0	39.0	30.0	39.0
11	35.859755891829884	37.0	35.0	39.0	31.0	39.0
12	35.72609865546502	37.0	35.0	39.0	31.0	39.0
13	35.57666464227272	37.0	35.0	39.0	30.0	39.0
14	36.6630438449989	38.0	35.0	40.0	31.0	41.0
15	36.752534709921214	38.0	35.0	40.0	31.0	41.0
16	36.72267068171466	38.0	35.0	40.0	31.0	41.0
17	36.652425898746564	38.0	35.0	40.0	31.0	41.0
18	36.44944076117213	38.0	35.0	40.0	31.0	41.0
19	36.394070212730064	38.0	35.0	40.0	30.0	41.0
20	36.311806434198765	38.0	34.0	40.0	30.0	41.0
21	36.30882804460415	38.0	35.0	40.0	30.0	41.0
22	36.2672503669214	38.0	35.0	40.0	30.0	41.0
23	36.15739156839921	38.0	35.0	40.0	30.0	41.0
24	35.79063211700997	38.0	34.0	40.0	29.0	41.0
25	35.782980751387555	38.0	34.0	40.0	29.0	41.0
26	35.6255411036321	38.0	34.0	40.0	29.0	41.0
27	35.478125579904514	38.0	34.0	40.0	27.0	41.0
28	35.31338630497495	38.0	34.0	40.0	27.0	41.0
29	35.05961840174098	37.0	33.0	40.0	26.0	41.0
30	35.081193380231795	37.0	34.0	40.0	26.0	41.0
31	34.851647350574424	37.0	33.0	40.0	25.0	41.0
32	34.8101337787	37.0	33.0	40.0	25.0	41.0
33	34.63682878688193	37.0	33.0	40.0	25.0	41.0
34	34.52282419825565	37.0	33.0	40.0	24.0	41.0
35	34.35407999730081	37.0	33.0	40.0	24.0	41.0
36	34.16643048737284	37.0	33.0	40.0	23.0	41.0
37	34.04582553098166	36.0	33.0	40.0	23.0	41.0
38	33.96573713244597	36.0	33.0	40.0	23.0	41.0
39	33.783213556691464	36.0	33.0	40.0	22.0	41.0
40	33.637717833223675	36.0	32.0	40.0	22.0	41.0
41	33.56351282959664	36.0	32.0	39.0	21.0	41.0
42	33.39012939251312	36.0	32.0	39.0	21.0	41.0
43	33.28876123960389	35.0	32.0	39.0	21.0	41.0
44	33.16840089748132	35.0	32.0	39.0	21.0	40.0
45	33.01940634647502	35.0	32.0	39.0	20.0	40.0
46	32.86262631374732	35.0	31.0	39.0	20.0	40.0
47	32.7687610709044	35.0	31.0	39.0	20.0	40.0
48	32.592589031158795	35.0	31.0	39.0	20.0	40.0
49	32.39650370295393	35.0	31.0	39.0	18.0	40.0
50	32.21494255782175	35.0	31.0	38.0	18.0	40.0
51	30.16339727044216	34.0	26.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	11.0
10	16.0
11	35.0
12	43.0
13	64.0
14	117.0
15	221.0
16	475.0
17	960.0
18	1773.0
19	3195.0
20	5085.0
21	7059.0
22	9618.0
23	12289.0
24	14410.0
25	16038.0
26	18335.0
27	21573.0
28	25859.0
29	31440.0
30	38397.0
31	47702.0
32	60709.0
33	81103.0
34	118082.0
35	121761.0
36	128980.0
37	159637.0
38	173833.0
39	86719.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.41938694603303	34.71093341430909	21.53870809926278	23.330971540395094
2	21.95775764630464	34.03487018573814	20.34296607453144	23.66440609342578
3	20.863825767160957	33.41228469726876	19.469946184860905	26.25394335070938
4	19.859557669922566	35.16085496904364	18.554329672554278	26.42525768847951
5	17.924996204261348	37.56423233294532	16.85670664844712	27.654064814346206
6	18.972451372370397	39.94584746191609	18.756262968773722	22.325438196939793
7	81.4942557821752	11.939116352042108	3.3539146717951316	3.21271319398755
8	83.97422271707407	9.874150176290973	2.8192216205273546	3.3324054861075965
9	78.0300116402652	13.351552878856893	4.968706243568332	3.6497292373095807
10	42.34812827909645	33.99775629670867	9.549994095517654	14.104121328677227
11	31.308348938036673	28.93483138485416	19.95622248089478	19.800597196214383
12	29.346795553081297	25.8602830777536	26.564013023601056	18.228908345564047
13	24.02188032457783	32.5896216070314	23.04974948124905	20.338748587141726
14	18.40174097879447	33.65293452772576	27.74027025659193	20.205054236887833
15	17.63694181554397	29.605243180322894	31.900990266039102	20.856824738094033
16	20.140864078816403	27.085631863960725	29.65323818681782	23.120265870405046
17	20.89444472561027	27.27820233817501	24.148151897025826	27.679201039188893
18	23.95541272331596	29.465813047218987	23.11739797898004	23.46137625048501
19	24.316851392614335	30.416687754103616	22.51994871535334	22.746512137928708
20	26.497208023348012	29.599338697977295	21.560132935202525	22.343320343472172
21	23.872834320225383	30.114378258009005	23.05405131838656	22.958736103379053
22	24.851038345395345	28.136376672233748	22.43214062789952	24.580444354471382
23	24.295763955665773	29.873812777299797	20.723467786831318	25.106955480203112
24	22.63010948597264	29.155912073822897	24.678964859895068	23.535013580309393
25	22.489245407156233	29.564586601886063	22.952072473303307	24.9940955176544
26	23.5055755183292	32.2798049833831	20.87242944143597	23.34219005685173
27	21.379540125175026	31.050744808273024	23.102636773116046	24.467078293435904
28	21.723096647941023	31.911871383504565	25.172748283482633	21.192283685071782
29	21.441537189803803	30.77112539433507	24.40077939166962	23.38655802419151
30	23.372809015301044	30.23120265870405	24.338023179310696	22.05796514668421
31	25.087048939723672	31.78129797391906	19.968959292811714	23.162693793545557
32	27.249945172663935	29.915481552710155	21.03581490291344	21.798758371712466
33	25.19273917370987	30.867958904802872	21.370346002665453	22.568955918821803
34	24.454425831266764	31.432258717546436	21.810398636908076	22.302916814278724
35	24.402888135364474	30.581338461797998	23.701604332203047	21.314169070634478
36	27.76953961907654	29.89118882534541	22.949710680365065	19.389560875212982
37	24.877608515950538	30.614740961924525	22.771648362771398	21.736002159353543
38	26.741737942203553	29.37598056581811	22.29785582941107	21.58442566256727
39	25.650673954484876	28.2105201005449	21.821954552355887	24.316851392614335
40	28.31005280294212	27.73942675911399	21.44389898274204	22.50662145520185
41	24.268856386119406	26.22340874200786	24.8341683958365	24.67356647603624
42	26.03589925266123	28.568500430183715	21.56561566880915	23.8299846483459
43	25.23440794912023	27.697504934460248	22.960001349595967	24.108085766823557
44	23.51367309411745	28.9973345479697	23.883715437690842	23.60527692022201
45	24.667915042934023	30.008181925536043	22.32636604416553	22.99753698736441
46	24.715994399176747	28.205712164920627	25.427231472577898	21.65106196332473
47	25.29050053140341	28.109384752939587	23.683553486175075	22.916561229481925
48	25.846196669871958	25.733168007827658	25.84290702970798	22.577728292592404
49	24.41343185383876	26.10278860266208	25.80064780606306	23.683131737436106
50	22.980667037805556	27.684852472291112	24.900973396089547	24.433507093813787
51	23.341430909121584	27.47887038817754	24.31398350118933	24.86571520151155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21780.0
1	17206.0
2	12632.0
3	8526.5
4	4421.0
5	3938.0
6	3455.0
7	3765.5
8	4076.0
9	4379.5
10	4683.0
11	4912.0
12	5141.0
13	5292.5
14	5444.0
15	5671.0
16	5898.0
17	5644.0
18	5390.0
19	5147.5
20	4905.0
21	4994.5
22	5084.0
23	5078.0
24	5072.0
25	5208.0
26	6851.5
27	8359.0
28	10047.5
29	11736.0
30	13428.0
31	15120.0
32	19006.0
33	22892.0
34	24409.0
35	25926.0
36	27482.0
37	29038.0
38	30814.5
39	32591.0
40	36314.0
41	40037.0
42	48697.0
43	57357.0
44	66044.0
45	74731.0
46	75921.5
47	77112.0
48	75998.0
49	74884.0
50	75328.5
51	75773.0
52	70913.0
53	66053.0
54	63427.5
55	60802.0
56	60701.0
57	60600.0
58	59506.0
59	58412.0
60	57614.5
61	56817.0
62	57543.5
63	58270.0
64	52542.5
65	46815.0
66	43809.0
67	40803.0
68	34957.0
69	29111.0
70	24713.5
71	20316.0
72	19011.0
73	17706.0
74	15246.5
75	10280.0
76	7773.0
77	5749.0
78	3725.0
79	3112.0
80	2499.0
81	2003.0
82	1507.0
83	1276.5
84	1046.0
85	829.0
86	612.0
87	522.5
88	433.0
89	409.0
90	385.0
91	244.5
92	104.0
93	81.0
94	58.0
95	39.0
96	20.0
97	12.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1185540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.96820049224313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.5079897643011	20.90647619426829
2	8.53264341150277	4.431547896774427
3	3.138481277836346	2.4450213319201697
4	1.6200388565663126	1.6827797533015332
5	0.9492609855503323	1.2325299796117672
6	0.5922643002669971	0.9228022856238879
7	0.4053077092437066	0.736757829828465
8	0.3010711182479007	0.6254620128868261
9	0.24848315490908188	0.5807394347061732
>10	2.064806535305775	12.229797683084723
>50	0.794823724254396	15.051413226566785
>100	0.8389074622815995	35.34615626295623
>500	0.0026318665483344066	0.406310063075076
>1k	0.0029608498668762077	1.8442000276706616
>5k	0.0	0.0
>10k+	3.289833185418008E-4	1.558006017725013
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18237	1.5382863505238118	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4292	0.3620291175329386	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3633	0.30644263373652514	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	2579	0.2175379995613813	Illumina Single End Adapter 2 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2575	0.21720060057020427	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGT	2217	0.18700339085986134	No Hit
AATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCT	2024	0.17072388953557027	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC	1975	0.16659075189365186	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1195	0.10079794861413363	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.434974779425409E-5	0.0	0.0	0.30644263373652514	0.0
2	8.434974779425409E-5	0.0	0.0	0.38193565801238255	0.0
3	8.434974779425409E-5	0.0	0.0	0.5001940044199268	0.0
4	8.434974779425409E-5	0.0	0.0	1.2055265954754795	0.0
5	8.434974779425409E-5	0.0	0.0	1.3339069116183342	0.0
6	8.434974779425409E-5	0.0	0.0	1.9010746157868987	0.0
7	8.434974779425409E-5	0.0	0.0	2.314135330735361	0.0
8	8.434974779425409E-5	0.0	0.0	2.6050576108777435	0.0
9	8.434974779425409E-5	0.0	0.0	3.222329065236095	0.0
10	8.434974779425409E-5	0.0	0.0	3.928673853265179	0.0
11	8.434974779425409E-5	0.0	0.0	4.710596015317914	0.0
12	8.434974779425409E-5	0.0	0.0	5.088482885436172	0.0
13	8.434974779425409E-5	0.0	0.0	5.269919192941613	0.0
14	8.434974779425409E-5	0.0	0.0	5.43878738802571	0.0
15	8.434974779425409E-5	0.0	0.0	5.558226630902373	0.0
16	8.434974779425409E-5	0.0	0.0	5.80461224420939	0.0
17	8.434974779425409E-5	0.0	0.0	6.09351013040471	0.0
18	8.434974779425409E-5	0.0	0.0	6.567302663765036	0.0
19	8.434974779425409E-5	0.0	0.0	6.7232653474366115	0.0
20	8.434974779425409E-5	0.0	0.0	6.938863302798724	0.0
21	8.434974779425409E-5	0.0	0.0	7.122661403242405	0.0
22	8.434974779425409E-5	0.0	0.0	7.325185147696408	0.0
23	8.434974779425409E-5	0.0	0.0	7.525515798707762	0.0
24	8.434974779425409E-5	0.0	0.0	7.664524183072692	0.0
25	8.434974779425409E-5	0.0	0.0	7.7804207365419975	0.0
26	8.434974779425409E-5	0.0	0.0	7.887966664979672	0.0
27	8.434974779425409E-5	0.0	0.0	8.020817517755622	0.0
28	8.434974779425409E-5	0.0	0.0	8.138401066180812	0.0
29	8.434974779425409E-5	0.0	0.0	8.258009008553065	0.0
30	8.434974779425409E-5	0.0	0.0	8.438011370346002	0.0
31	8.434974779425409E-5	0.0	0.0	8.566897784975623	0.0
32	8.434974779425409E-5	0.0	0.0	8.701688681950841	0.0
33	8.434974779425409E-5	0.0	0.0	8.830068998093695	0.0
34	8.434974779425409E-5	0.0	0.0	8.96022065894023	0.0
35	8.434974779425409E-5	0.0	0.0	9.107157919597821	0.0
36	8.434974779425409E-5	0.0	0.0	9.237731329183326	0.0
37	8.434974779425409E-5	0.0	0.0	9.37066653170707	0.0
38	8.434974779425409E-5	0.0	0.0	9.507650522124939	0.0
39	8.434974779425409E-5	0.0	0.0	9.649020699428108	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	35	1.2119744E-7	45.0	1
TTAGGCG	25	3.8908798E-5	45.0	1
AGCGTAA	25	3.8908798E-5	45.0	24
ACTATCG	35	1.2119744E-7	45.0	1
AACCGCA	40	6.8157533E-9	45.0	1
TCGCATA	25	3.8908798E-5	45.0	30
CGAGTGA	20	7.033377E-4	45.0	24
TTTACGT	35	1.2119744E-7	45.0	38
CGAAACG	20	7.033377E-4	45.0	1
CGCATGT	40	6.8157533E-9	45.0	1
GAATCGT	25	3.8908798E-5	45.0	26
CGACCGA	35	1.2119744E-7	45.0	13
ATTAACG	35	1.2119744E-7	45.0	1
CAATTCG	20	7.033377E-4	45.0	1
TAAGTCG	25	3.8908798E-5	45.0	1
TCGATAG	20	7.033377E-4	45.0	1
ATCGTAG	85	0.0	45.0	1
CTACGTG	25	3.8908798E-5	45.0	1
ATATACG	45	3.8562575E-10	45.0	1
CACTACG	50	2.1827873E-11	45.0	1
>>END_MODULE