FastQCFastQC Report
Sat 18 Jun 2016
SRR3548353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279597
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA233861.8276066605345278No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57970.4530332596903557No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46780.36558385179083724No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTT43770.3420608207115209No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32060.2505476333564396No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTAT19500.15239172958361108No Hit
AATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGT18690.14606161158552264No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGT16620.12988464336818545No Hit
TCTACTGGGATACAAACACATACACACACACACATACACATATACA15950.1246486198389024No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT13200.1031574784873675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG507.8216544E-1140.0000041
AACCGTA304.8679467E-640.00000432
ACTATCG304.8679467E-640.0000041
CGTATTC257.795148E-540.00000412
CGAAACG507.8216544E-1140.0000041
ATAGCCG600.040.0000041
ACCGAAC257.795148E-540.00000410
TACGGCA304.8679467E-640.00000419
TAGCCGT304.8679467E-640.00000440
CCGTACT257.795148E-540.00000420
CTCGGTA304.8679467E-640.00000431
GTCGTGA257.795148E-540.0000049
ACCACGT257.795148E-540.0000049
CGTAAAC304.8679467E-640.00000425
GCGCTAT304.8679467E-640.00000427
TATCGAG257.795148E-540.0000041
ATACCCG257.795148E-540.00000437
CTAGACG353.056739E-740.01
GTACCGT200.001255822440.034
ACCGGAC200.001255822440.032