##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113687
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.062394550713083	31.0	31.0	34.0	28.0	34.0
2	31.358269423994354	31.0	31.0	34.0	28.0	34.0
3	31.540725535989914	31.0	31.0	34.0	30.0	34.0
4	35.48899017407943	37.0	35.0	37.0	33.0	37.0
5	35.062002160391565	37.0	35.0	37.0	32.0	37.0
6	35.20650236556591	37.0	35.0	37.0	32.0	37.0
7	35.17283761056742	37.0	35.0	37.0	32.0	37.0
8	35.56912399983119	37.0	35.0	37.0	35.0	37.0
9	36.907024145922506	39.0	37.0	39.0	33.0	39.0
10	36.20468408089526	38.0	35.0	39.0	32.0	39.0
11	36.362914355649295	38.0	35.0	39.0	32.0	39.0
12	36.041739734772875	37.0	35.0	39.0	31.0	39.0
13	35.65873714966593	37.0	35.0	39.0	30.0	39.0
14	36.84533895071057	39.0	35.0	40.0	31.0	41.0
15	36.79291129374771	38.0	35.0	40.0	31.0	41.0
16	36.754862901335834	38.0	35.0	40.0	31.0	41.0
17	36.697335966029954	38.0	35.0	40.0	31.0	41.0
18	36.510396547683506	38.0	35.0	40.0	31.0	41.0
19	36.70386293455881	38.0	35.0	40.0	31.0	41.0
20	36.450271934574076	38.0	35.0	40.0	31.0	41.0
21	36.52599159368835	38.0	35.0	40.0	31.0	41.0
22	36.62771586630714	38.0	35.0	40.0	31.0	41.0
23	36.42474052404311	38.0	35.0	40.0	31.0	41.0
24	36.016812623295415	38.0	34.0	40.0	30.0	41.0
25	36.20068026294641	38.0	35.0	40.0	30.0	41.0
26	36.18637911729238	38.0	35.0	40.0	30.0	41.0
27	36.050274448745476	38.0	35.0	40.0	30.0	41.0
28	36.0134553065628	38.0	35.0	40.0	30.0	41.0
29	35.7306963267058	38.0	34.0	40.0	29.0	41.0
30	35.75874729614335	38.0	34.0	40.0	30.0	41.0
31	35.66442636036876	38.0	34.0	40.0	29.0	41.0
32	35.61062399040305	38.0	34.0	40.0	29.0	41.0
33	35.54534353009418	38.0	34.0	40.0	29.0	41.0
34	35.45312102951727	38.0	34.0	40.0	29.0	41.0
35	35.17155987274701	37.0	34.0	40.0	27.0	41.0
36	35.09766119205845	37.0	34.0	40.0	27.0	41.0
37	35.0070307007265	37.0	34.0	40.0	27.0	41.0
38	34.816669315525814	37.0	34.0	40.0	26.0	41.0
39	34.78531221070193	37.0	34.0	40.0	26.0	41.0
40	34.54508492960769	36.0	33.0	40.0	24.0	41.0
41	34.573195161656734	36.0	33.0	40.0	25.0	41.0
42	34.42907567386528	36.0	33.0	40.0	24.0	41.0
43	34.32572616902235	36.0	33.0	40.0	24.0	41.0
44	34.20208999476514	36.0	33.0	40.0	24.0	41.0
45	33.974127380493805	35.0	33.0	40.0	23.0	41.0
46	32.837066428897884	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	6.0
9	12.0
10	20.0
11	20.0
12	27.0
13	41.0
14	52.0
15	115.0
16	198.0
17	332.0
18	722.0
19	1223.0
20	2187.0
21	3555.0
22	5483.0
23	7394.0
24	9595.0
25	11560.0
26	13361.0
27	15531.0
28	18264.0
29	22113.0
30	27780.0
31	35994.0
32	47475.0
33	67158.0
34	111377.0
35	119447.0
36	111317.0
37	148988.0
38	202531.0
39	129806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.09748968965248	32.09851601033324	18.74925360536668	28.054740694647602
2	23.14429458187085	32.907989408155075	19.168042726547046	24.77967328342703
3	19.782039298294762	34.42933247851506	18.911148284931045	26.877479938259135
4	20.26682541863199	35.320875614063915	17.686926398530286	26.725372568773814
5	18.98378987992138	36.50074033368442	16.862008804987397	27.653460981406802
6	19.436430523118258	37.985089167782334	18.403914205696932	24.17456610340248
7	82.57050679409924	9.989072333608995	3.7960396412995747	3.644381230992191
8	83.77075426039812	9.44816631602955	3.29114014979074	3.489939273781592
9	79.34419634960271	11.642140026775925	5.565657137059156	3.448006486562203
10	42.195069171140545	33.81084631498796	9.969138546108557	14.024945967762935
11	34.56734252981313	30.307797433210588	18.98450821460608	16.1403518223702
12	31.62091323684303	27.690365425833292	22.81233416570365	17.876387171620035
13	24.376777317145663	34.76120310284667	21.368481449455725	19.49353813055194
14	18.353092026754375	35.43096040449426	24.4154775982839	21.800469970467464
15	18.16542709037638	30.642002645267475	30.861902850621405	20.330667413734737
16	20.75116257979127	27.654718067105033	30.03123857960091	21.562880773502787
17	20.51554880320952	28.660027458343322	24.682787892828056	26.1416358456191
18	24.998226611247144	29.076481991798413	23.32549450608654	22.5997968908679
19	24.834895262313378	31.77203289613689	21.11921931386467	22.27385252768507
20	28.157552346395352	30.251228576790428	20.859361741674277	20.731857335139946
21	23.39014462770958	31.974962444564763	22.750916550161758	21.883976377563894
22	24.18372487063241	28.12235394684503	21.906334544625196	25.787586637897363
23	22.273493360342716	33.146656107146796	21.257139573327155	23.322710959183325
24	20.389930025222526	30.6807927182413	25.94023275839621	22.98904449813996
25	21.534326969785944	31.489727365049607	23.19215363023902	23.783792034925433
26	22.90374225433178	33.886181665045925	20.479003526125382	22.731072554496908
27	21.36057976792402	31.81432485069863	22.70045353856155	24.1246418428158
28	20.486366456643562	32.585546926560156	26.121522474447488	20.806564142348794
29	20.88459324747438	31.289671155360526	24.759290536748654	23.066445060416438
30	23.77571076972255	28.901657287909437	25.182659041543985	22.13997290082402
31	27.390640278642024	29.646121396765878	21.630853193042572	21.33238513154953
32	26.97975283899336	27.990180364860144	22.40054880769911	22.629517988447382
33	24.139547287523335	28.74838262456148	22.13036517441615	24.981704913499037
34	23.044446060697485	29.73241135076552	24.258880637019196	22.9642619515178
35	23.727043594834097	28.89968186752651	25.49585296407339	21.877421573566004
36	25.10265451603548	29.438073713709507	23.94936817974889	21.50990359050613
37	23.44752161065003	30.760438076407464	22.93956919673122	22.852471116211287
38	24.541455543613242	29.2302954061599	23.78397161859661	22.44427743163025
39	23.2990957064238	27.55917955403987	24.518468833702826	24.623255905833506
40	26.58269334202518	26.912049794960343	23.689420815722908	22.815836047291562
41	21.760512603631003	25.81470377224481	26.352467075578684	26.072316548545505
42	27.69988336040557	25.84613091470045	22.590458539966797	23.863527184927182
43	25.143599593063403	26.63971115762328	24.350917268496445	23.86577198081687
44	25.017621647734057	27.40446822132251	24.29264236720012	23.285267763743313
45	23.91731249444413	28.962176985095457	23.213164919766506	23.907345600693912
46	22.797608304667293	28.052585690593496	25.556911412272925	23.592894592466283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24409.0
1	19860.0
2	15311.0
3	10552.0
4	5793.0
5	5354.5
6	4916.0
7	4916.0
8	5013.5
9	5111.0
10	5083.0
11	5055.0
12	5002.5
13	4950.0
14	4799.0
15	4648.0
16	4864.0
17	5080.0
18	4617.0
19	4154.0
20	4154.0
21	4253.0
22	4352.0
23	4365.0
24	4378.0
25	4662.0
26	4946.0
27	5134.5
28	5323.0
29	9048.0
30	12773.0
31	12773.0
32	13820.0
33	14867.0
34	18676.5
35	22486.0
36	23623.0
37	24760.0
38	29667.5
39	34575.0
40	39671.5
41	44768.0
42	52086.5
43	59405.0
44	59405.0
45	67852.5
46	76300.0
47	83365.5
48	90431.0
49	89299.0
50	88167.0
51	81962.5
52	75758.0
53	72303.5
54	68849.0
55	65225.0
56	61601.0
57	61601.0
58	60763.5
59	59926.0
60	58788.5
61	57651.0
62	55300.5
63	52950.0
64	52238.5
65	51527.0
66	43370.5
67	35214.0
68	30615.5
69	26017.0
70	26017.0
71	24781.0
72	23545.0
73	18570.5
74	13596.0
75	11020.0
76	8444.0
77	6773.0
78	5102.0
79	4084.0
80	3066.0
81	3066.0
82	2616.0
83	2166.0
84	1523.0
85	880.0
86	624.0
87	368.0
88	202.5
89	37.0
90	22.5
91	8.0
92	8.5
93	9.0
94	9.0
95	7.0
96	5.0
97	5.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1113687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.071549848284093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52117436573074	14.593773913652727
2	9.940602692202232	3.7916539953264374
3	3.687032194080664	2.1095225484491293
4	1.8353324092350236	1.4001053412358837
5	1.1657209498686603	1.1116052602304616
6	0.7840715450537569	0.8972075733668311
7	0.5612410906718306	0.7492616206357203
8	0.40195914560880425	0.6132787105961595
9	0.3174132150200335	0.5448205757482816
>10	2.5340917560528258	10.888000227811089
>50	0.8890814043065227	12.486762175952675
>100	1.348069087188034	45.79653838959799
>500	0.009947101486629906	1.2474566748961124
>1k	0.0033157004955433026	1.4358082693025427
>5k	4.736714993633289E-4	0.47255021119426205
>10k+	4.736714993633289E-4	1.8616545120036991
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20608	1.8504301477883824	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5231	0.46970109195851256	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4196	0.37676654212539074	No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTT	3298	0.2961334737677642	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2448	0.2198104135183404	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTAT	1637	0.1469892348568314	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGT	1614	0.1449240226383176	No Hit
AGCACAGGGAAACCAGAAGACCAACACGCGGGCGACCGCGCGGGGG	1491	0.13387962686104804	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGT	1210	0.10864812106094442	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7958367117511473E-4	0.0	0.0	0.3427354364377065	0.0
2	1.7958367117511473E-4	0.0	0.0	0.46305649612503336	0.0
3	1.7958367117511473E-4	0.0	0.0	0.6080703105989385	0.0
4	1.7958367117511473E-4	0.0	0.0	1.3275722891620356	0.0
5	1.7958367117511473E-4	0.0	0.0	1.4652231731177612	0.0
6	1.7958367117511473E-4	0.0	0.0	2.160840523414568	0.0
7	1.7958367117511473E-4	0.0	0.0	2.697795700228161	0.0
8	1.7958367117511473E-4	0.0	0.0	3.093328735991351	0.0
9	2.693755067626721E-4	0.0	0.0	3.736058695127087	0.0
10	3.5916734235022946E-4	0.0	0.0	4.655527091543674	0.0
11	3.5916734235022946E-4	0.0	0.0	5.505137439873142	0.0
12	3.5916734235022946E-4	0.0	0.0	5.9485295240045	0.0
13	3.5916734235022946E-4	0.0	0.0	6.1283825706863775	0.0
14	3.5916734235022946E-4	0.0	0.0	6.300244144000962	0.0
15	3.5916734235022946E-4	0.0	0.0	6.438343987134626	0.0
16	3.5916734235022946E-4	0.0	0.0	6.690659045135662	0.0
17	3.5916734235022946E-4	0.0	0.0	7.017052367496433	0.0
18	3.5916734235022946E-4	0.0	0.0	7.4290173091721465	0.0
19	3.5916734235022946E-4	0.0	0.0	7.600340131473206	0.0
20	3.5916734235022946E-4	0.0	0.0	7.787915276015613	0.0
21	3.5916734235022946E-4	0.0	0.0	7.9762087552427205	0.0
22	3.5916734235022946E-4	0.0	0.0	8.170967246632133	0.0
23	3.5916734235022946E-4	0.0	0.0	8.371831582841498	0.0
24	3.5916734235022946E-4	0.0	0.0	8.50813558926341	0.0
25	3.5916734235022946E-4	0.0	0.0	8.645337514041199	0.0
26	3.5916734235022946E-4	0.0	0.0	8.769070663480852	0.0
27	3.5916734235022946E-4	0.0	0.0	8.908158216805978	0.0
28	4.489591779377868E-4	0.0	0.0	9.038895129421462	0.0
29	4.489591779377868E-4	0.0	0.0	9.183729360224191	0.0
30	4.489591779377868E-4	0.0	0.0	9.41467396135539	0.0
31	4.489591779377868E-4	0.0	0.0	9.55250442898229	0.0
32	4.489591779377868E-4	0.0	0.0	9.701289500550873	0.0
33	4.489591779377868E-4	0.0	0.0	9.841903515080988	0.0
34	4.489591779377868E-4	0.0	0.0	9.967612084903568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGATG	75	0.0	40.000004	5
CAGGTCG	25	7.794498E-5	40.0	33
TAGAGTA	20	0.001255752	40.0	15
AAGTAGT	20	0.001255752	40.0	36
CTATGCG	50	7.8216544E-11	40.0	1
AGCGTAA	25	7.794498E-5	40.0	11
TCGTTAC	20	0.001255752	40.0	36
CTCACCG	50	7.8216544E-11	40.0	1
TCACCGA	25	7.794498E-5	40.0	25
CTACTCG	30	4.8673774E-6	40.0	14
GGTAAGT	30	4.8673774E-6	40.0	5
ATCTAAT	40	1.9281288E-8	40.0	12
CGAAATG	45	1.2223609E-9	40.0	1
CGAAATA	20	0.001255752	40.0	13
CGAAAGA	40	1.9281288E-8	40.0	15
CGAAACG	90	0.0	40.0	1
GCGAATC	20	0.001255752	40.0	21
CTATCGC	20	0.001255752	40.0	33
GACACGT	50	7.8216544E-11	40.0	10
TAGGTAC	25	7.794498E-5	40.0	35
>>END_MODULE