##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	602302
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02869324690936	31.0	31.0	33.0	28.0	34.0
2	31.17864626051383	31.0	31.0	34.0	28.0	34.0
3	31.415705078183368	31.0	31.0	34.0	30.0	34.0
4	35.34887149635897	37.0	35.0	37.0	33.0	37.0
5	34.91075407353786	35.0	35.0	37.0	32.0	37.0
6	34.86860246188789	35.0	35.0	37.0	32.0	37.0
7	34.04274931844822	35.0	35.0	37.0	30.0	37.0
8	34.40066445072406	36.0	35.0	37.0	31.0	37.0
9	35.529644596896574	38.0	35.0	39.0	30.0	39.0
10	35.23489047022922	37.0	34.0	39.0	29.0	39.0
11	35.646006488439355	37.0	35.0	39.0	30.0	39.0
12	35.48023250794452	37.0	35.0	39.0	30.0	39.0
13	35.302746794797294	37.0	35.0	39.0	30.0	39.0
14	36.30689089526516	38.0	35.0	40.0	30.0	41.0
15	36.451535940441836	38.0	35.0	40.0	31.0	41.0
16	36.403309303306315	38.0	35.0	40.0	31.0	41.0
17	36.34769932691573	38.0	35.0	40.0	31.0	41.0
18	36.07389316323041	37.0	35.0	40.0	30.0	41.0
19	35.90291747329413	37.0	34.0	40.0	30.0	41.0
20	35.83028945612002	37.0	34.0	40.0	30.0	41.0
21	35.87656690497458	37.0	34.0	40.0	30.0	41.0
22	35.831410156366736	37.0	34.0	40.0	30.0	41.0
23	35.68817470305594	37.0	34.0	40.0	29.0	41.0
24	35.28086408479467	37.0	34.0	40.0	27.0	41.0
25	35.35209745277286	37.0	34.0	40.0	28.0	41.0
26	35.2738509916952	37.0	34.0	40.0	27.0	41.0
27	35.084675129752185	37.0	33.0	40.0	27.0	41.0
28	35.059911805041324	37.0	33.0	40.0	27.0	41.0
29	34.8461967584368	37.0	33.0	40.0	26.0	41.0
30	34.87293749647187	37.0	33.0	40.0	26.0	41.0
31	34.65712549518348	37.0	33.0	40.0	25.0	41.0
32	34.57160361413377	37.0	33.0	40.0	25.0	41.0
33	34.47639556235908	37.0	33.0	40.0	24.0	41.0
34	34.439427396887275	37.0	33.0	40.0	24.0	41.0
35	34.31491344873502	37.0	33.0	40.0	24.0	41.0
36	34.189292082709336	37.0	33.0	40.0	23.0	41.0
37	34.01551049141461	36.0	33.0	40.0	23.0	41.0
38	33.92014803205036	36.0	33.0	40.0	23.0	41.0
39	33.72370007072864	36.0	33.0	40.0	21.0	41.0
40	33.48444468057553	36.0	33.0	40.0	21.0	41.0
41	33.47780681452162	36.0	33.0	40.0	21.0	41.0
42	33.36756809706758	36.0	33.0	40.0	20.0	41.0
43	33.34648565005595	36.0	33.0	39.0	20.0	41.0
44	33.21837051844424	35.0	32.0	39.0	20.0	40.0
45	33.01401290382566	35.0	32.0	39.0	20.0	40.0
46	32.86681598267978	35.0	31.0	39.0	18.0	40.0
47	32.75140378082756	35.0	31.0	39.0	18.0	40.0
48	32.635051851064745	35.0	31.0	39.0	18.0	40.0
49	32.49133325142537	35.0	31.0	39.0	15.0	40.0
50	32.27068314566447	35.0	31.0	39.0	15.0	40.0
51	30.186122576381948	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	8.0
12	15.0
13	21.0
14	46.0
15	78.0
16	204.0
17	443.0
18	969.0
19	1784.0
20	2912.0
21	4292.0
22	5658.0
23	7141.0
24	8214.0
25	8724.0
26	9807.0
27	11134.0
28	13146.0
29	15995.0
30	19661.0
31	25001.0
32	32486.0
33	42509.0
34	63387.0
35	66305.0
36	61211.0
37	75931.0
38	84242.0
39	40971.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.02152076533035	39.10098256356446	19.011891044691865	22.865605626413327
2	17.76517428134059	39.67195858556007	20.7351129499819	21.827754183117438
3	17.78459975228374	37.39685406988521	20.984655538251577	23.83389063957948
4	19.716686977629163	37.38058316259949	17.504673735102987	25.398056124668354
5	15.72632998064094	41.47487473061687	16.856327888667145	25.94246740007505
6	18.090426397388683	41.70200331395213	20.328174238172878	19.8793960504863
7	71.79471427954746	18.53073707210004	5.457229097695176	4.217319550657312
8	74.99692845117566	15.212302134145329	3.769039451969278	6.0217299627097365
9	69.31572533380265	19.168623049566495	7.299992362635356	4.215659253995504
10	34.63196203897713	41.553406762720364	11.09443435353029	12.720196844772225
11	27.88302213839569	32.26354885090868	25.87373111827621	13.979697892419418
12	27.109157864327198	31.370143217190048	26.689600897888432	14.831098020594318
13	20.32269525918891	40.125219574233526	23.05421532719466	16.4978698393829
14	15.184243120560781	41.57083987766934	27.13788099657647	16.107036005193407
15	13.075666360065217	37.48601200062427	31.608395788159427	17.829925851151085
16	15.504514346623456	34.7279271860296	31.263884230834364	18.50367423651258
17	15.58038990406806	33.974318531235156	25.164286354685856	25.281005210010925
18	17.896337717623386	35.0375725134567	25.470611088789347	21.59547868013057
19	19.87607545716269	36.77557105903683	25.74107341499779	17.607280068802694
20	20.569581372799693	37.37942095493623	23.625523408522632	18.425474263741446
21	17.702415067524267	36.13967743756454	25.17192371933017	20.985983775581023
22	17.52293699838287	36.48003825323509	23.723148852236918	22.273875896145125
23	17.58868474619045	36.73705217648289	22.25345424720489	23.420808830121768
24	15.061713226919386	38.60222944635748	26.732934640761613	19.603122685961527
25	15.84985605227942	36.662172797035375	25.885187165242684	21.60278398544252
26	17.398414748747307	39.847285913046946	22.529561582063483	20.224737756142268
27	15.31158787452142	38.340566692456605	25.294287583305387	21.053557849716587
28	16.28800834133043	35.838167563780296	29.30290784357349	18.570916251315786
29	15.522611580237156	36.52088155111555	27.92535970327178	20.03114716537551
30	17.265591015802702	35.54047637231821	25.9678699390007	21.226062672878392
31	21.74839200268304	36.47090662159515	24.045080374961397	17.735621000760414
32	19.91642066604461	33.96468881059668	22.60377684284628	23.515113680512435
33	20.20531228519912	35.0772536036739	25.585669647452608	19.13176446367437
34	17.4502160045957	33.56488937443343	26.057858018070668	22.927036602900206
35	16.673031137203594	32.31036921677165	26.641452294696016	24.375147351328735
36	21.001756593868194	30.681618191538462	29.46578294609681	18.850842268496535
37	17.880564899336214	33.79301413576578	24.43358979382436	23.89283117107365
38	19.16281201125017	32.54098442309672	27.4447038196785	20.85149974597461
39	20.001427855129155	31.302735172720663	26.392573825091066	22.303263147059116
40	21.330993421904626	30.98777689597577	24.272042928630487	23.409186753489113
41	18.83639768753881	29.13588199939565	28.69225073136068	23.33546958170486
42	22.385945920817132	30.163605633054512	23.899140298388517	23.55130814773984
43	20.029652898379883	30.053361934710495	26.571221745901557	23.345763421008066
44	19.677670006076685	30.868235536325635	25.486383907076515	23.96771055052117
45	20.72813970400231	34.43156423189696	24.542837314171294	20.297458749929437
46	18.807010436624815	31.369147039192963	29.678632978140534	20.145209546041688
47	19.97818370186385	32.02762069526583	25.659552848903044	22.33464275396728
48	21.037785031429415	27.646761923420478	30.115291000195914	21.200162044954194
49	20.04044482668163	27.32150980737238	27.791041703331555	24.847003662614437
50	19.163144070582533	30.1646018110516	25.523906611633368	25.148347506732506
51	17.664062214636512	30.469432278159463	28.267712874936496	23.598792632267536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23310.0
1	18159.0
2	13008.0
3	8506.0
4	4004.0
5	3541.0
6	3078.0
7	3312.5
8	3547.0
9	3742.5
10	3938.0
11	4397.5
12	4857.0
13	4886.5
14	4916.0
15	4850.5
16	4785.0
17	4744.5
18	4704.0
19	4594.5
20	4485.0
21	4457.0
22	4429.0
23	4281.5
24	4134.0
25	4507.0
26	4900.5
27	4921.0
28	6611.5
29	8302.0
30	9378.5
31	10455.0
32	10836.5
33	11218.0
34	13109.0
35	15000.0
36	16561.5
37	18123.0
38	19230.5
39	20338.0
40	23170.5
41	26003.0
42	31880.5
43	37758.0
44	49058.5
45	60359.0
46	60231.5
47	60104.0
48	56101.5
49	52099.0
50	47007.5
51	41916.0
52	39207.0
53	36498.0
54	31299.0
55	26100.0
56	22928.0
57	19756.0
58	17619.5
59	15483.0
60	14759.0
61	14035.0
62	12518.5
63	11002.0
64	9799.0
65	8596.0
66	6988.5
67	5381.0
68	4540.5
69	3700.0
70	3463.0
71	3226.0
72	2429.5
73	1633.0
74	1262.5
75	703.5
76	515.0
77	483.5
78	452.0
79	304.0
80	156.0
81	120.0
82	84.0
83	71.0
84	58.0
85	38.0
86	18.0
87	14.5
88	11.0
89	10.0
90	9.0
91	8.5
92	8.0
93	8.0
94	8.0
95	6.0
96	4.0
97	5.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	602302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.65951067444314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81455941393955	25.268898957415953
2	8.914984449502999	5.644880906830696
3	3.199375413032938	3.038719801214017
4	1.6345678937964792	2.0699847872700667
5	0.9445602598776082	1.495215781512496
6	0.6814377918322105	1.29443922266885
7	0.4289268573427819	0.9505730093019407
8	0.34055752428303243	0.8625507660240475
9	0.2663688231273606	0.7589795939225578
>10	2.7503264245968913	19.401428280203998
>50	0.7156973519154074	16.12759173723009
>100	0.3011491846998309	13.902710914795557
>500	0.001604702582769969	0.3310733755411385
>1k	0.004814107748309907	4.543071839203132
>5k	5.34900860923323E-4	0.9395371291571544
>10k+	5.34900860923323E-4	3.370343897708307
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19902	3.30432241632935	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGT	5548	0.9211325879708185	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4835	0.8027534359839417	No Hit
AATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	4427	0.7350133321821943	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4057	0.6735823556953157	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC	4021	0.6676052877128086	No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	3416	0.5671573396734528	Illumina Single End Adapter 1 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2639	0.4381522890510076	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	1392	0.23111329532360841	No Hit
ATCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTTCTCTGCTC	1032	0.17134261549853727	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT	1008	0.1673579035101992	No Hit
AAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	699	0.11605473666034646	TruSeq Adapter, Index 23 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	635	0.10542883802477827	No Hit
AACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	621	0.10310442269824771	TruSeq Adapter, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7614120491049341	0.0
2	0.0	0.0	0.0	0.9589873518600304	0.0
3	0.0	0.0	0.0	1.3025027311880086	0.0
4	0.0	0.0	0.0	3.287387390378913	0.0
5	0.0	0.0	0.0	3.5472238179517914	0.0
6	0.0	0.0	0.0	4.801577945947382	0.0
7	0.0	0.0	0.0	5.70710374529721	0.0
8	0.0	0.0	0.0	6.1884237475552135	0.0
9	0.0	0.0	0.0	8.018568757865655	0.0
10	0.0	0.0	0.0	9.292514386470575	0.0
11	0.0	0.0	0.0	11.252494595734365	0.0
12	0.0	0.0	0.0	11.93487652373726	0.0
13	0.0	0.0	0.0	12.285531178711013	0.0
14	0.0	0.0	0.0	12.642827020331993	0.0
15	0.0	0.0	0.0	12.892037549269304	0.0
16	0.0	0.0	0.0	13.390458607143925	0.0
17	0.0	0.0	0.0	13.941179009865483	0.0
18	0.0	0.0	0.0	14.864802042829012	0.0
19	0.0	0.0	0.0	15.18175267556807	0.0
20	0.0	0.0	0.0	15.595498603690507	0.0
21	0.0	0.0	0.0	15.93536133036251	0.0
22	0.0	0.0	0.0	16.325531045887278	0.0
23	0.0	0.0	0.0	16.711051930758988	0.0
24	0.0	0.0	0.0	16.972548654993673	0.0
25	0.0	0.0	0.0	17.201337534990753	0.0
26	0.0	0.0	0.0	17.425975673333312	0.0
27	0.0	0.0	0.0	17.668877074955752	0.0
28	0.0	0.0	0.0	17.90663155692659	0.0
29	0.0	0.0	0.0	18.156838263860987	0.0
30	0.0	0.0	0.0	18.527084419444066	0.0
31	1.6602966618075319E-4	0.0	0.0	18.78858114367875	0.0
32	1.6602966618075319E-4	0.0	0.0	19.018200172006733	0.0
33	1.6602966618075319E-4	0.0	0.0	19.247985230000896	0.0
34	1.6602966618075319E-4	0.0	0.0	19.489890453626256	0.0
35	1.6602966618075319E-4	0.0	0.0	19.74690437687406	0.0
36	1.6602966618075319E-4	0.0	0.0	20.013381991094167	0.0
37	1.6602966618075319E-4	0.0	0.0	20.259437956374043	0.0
38	1.6602966618075319E-4	0.0	0.0	20.497856557009605	0.0
39	1.6602966618075319E-4	0.0	0.0	20.757194895583943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	25	3.888586E-5	45.000004	1
TGAAGCG	25	3.888586E-5	45.000004	1
GCGCCCG	25	3.888586E-5	45.000004	13
CGATTGG	25	3.888586E-5	45.000004	2
CCGACAG	25	3.888586E-5	45.000004	1
TAGTGCG	25	3.888586E-5	45.000004	1
CGTCCTA	25	3.888586E-5	45.000004	32
TTCGAGT	25	3.888586E-5	45.000004	14
ATCGCAT	25	3.888586E-5	45.000004	20
GACTCGT	25	3.888586E-5	45.000004	9
GTCGGTG	25	3.888586E-5	45.000004	2
TATCCGG	85	0.0	45.000004	2
GCGAGAC	20	7.030609E-4	45.0	21
CGTGGTT	20	7.030609E-4	45.0	38
CTAGACG	20	7.030609E-4	45.0	1
CGGGTCG	20	7.030609E-4	45.0	6
CGGAACC	20	7.030609E-4	45.0	6
TCGTTCG	20	7.030609E-4	45.0	45
GTCGCGG	40	6.8066583E-9	45.0	20
GTCGCAT	20	7.030609E-4	45.0	45
>>END_MODULE