FastQCFastQC Report
Sat 18 Jun 2016
SRR3548348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740562
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192262.596136447724836No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGT65470.8840583232734058No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC62460.8434135156813339No Hit
AATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT51740.6986585863168783No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47310.6388391518873504No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37430.5054269595253335No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT32350.43683040717725186No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22480.3035532473985973No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT13430.18134875945565665No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT11690.1578530899506051No Hit
AACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG8590.11599298910827183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAC551.8189894E-1245.00000422
TCCTATC302.1644519E-645.00000443
ACGTAAG302.1644519E-645.0000041
TTCGCGT302.1644519E-645.00000432
GCGTATT302.1644519E-645.00000412
TCACGGA207.0316595E-445.03
CGGGTCA253.8894563E-545.06
CGGGTAT253.8894563E-545.06
GCCCATA207.0316595E-445.041
ACACGTA453.8380676E-1045.038
TCGCACG207.0316595E-445.016
AGATCCG502.1827873E-1145.01
TACTTCG207.0316595E-445.013
ATCTACG253.8894563E-545.01
ACGTTAT351.2111377E-745.032
GCGGTAG207.0316595E-445.01
TCCGACA453.8380676E-1045.015
TCCGAAC207.0316595E-445.031
ATTACCG207.0316595E-445.01
ATAGCCG253.8894563E-545.01