##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548348_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740562
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.011619553798333	31.0	31.0	33.0	28.0	34.0
2	31.18027795106959	31.0	31.0	34.0	28.0	34.0
3	31.399905747256813	31.0	31.0	34.0	30.0	34.0
4	35.33370332261175	37.0	35.0	37.0	33.0	37.0
5	34.883368306772425	35.0	35.0	37.0	32.0	37.0
6	34.86792733086494	35.0	35.0	37.0	32.0	37.0
7	34.107024124921345	35.0	35.0	37.0	30.0	37.0
8	34.46829299910068	36.0	35.0	37.0	32.0	37.0
9	35.62089062090683	39.0	35.0	39.0	31.0	39.0
10	35.21163791822967	37.0	34.0	39.0	29.0	39.0
11	35.6338010862021	37.0	35.0	39.0	30.0	39.0
12	35.44672289423438	37.0	35.0	39.0	30.0	39.0
13	35.2792203758767	37.0	35.0	39.0	30.0	39.0
14	36.26614111985222	38.0	35.0	40.0	30.0	41.0
15	36.44768432622792	38.0	35.0	40.0	31.0	41.0
16	36.36259084317046	38.0	35.0	40.0	31.0	41.0
17	36.33657006435653	38.0	35.0	40.0	31.0	41.0
18	36.08718378744791	38.0	35.0	40.0	30.0	41.0
19	35.98844121086418	38.0	34.0	40.0	30.0	41.0
20	35.89171602107589	37.0	34.0	40.0	30.0	41.0
21	35.98069304123085	38.0	34.0	40.0	30.0	41.0
22	35.89929675030585	38.0	34.0	40.0	30.0	41.0
23	35.80230824698	38.0	34.0	40.0	30.0	41.0
24	35.39778168472052	37.0	34.0	40.0	27.0	41.0
25	35.455296112952055	37.0	34.0	40.0	29.0	41.0
26	35.34507036547919	37.0	34.0	40.0	28.0	41.0
27	35.19240387705553	37.0	33.0	40.0	27.0	41.0
28	35.1127170986359	37.0	33.0	40.0	27.0	41.0
29	34.92134487051725	37.0	33.0	40.0	26.0	41.0
30	34.91705488534383	37.0	33.0	40.0	26.0	41.0
31	34.691514012331176	37.0	33.0	40.0	25.0	41.0
32	34.62192632082122	37.0	33.0	40.0	25.0	41.0
33	34.52446115247609	37.0	33.0	40.0	25.0	41.0
34	34.52946410968967	37.0	33.0	40.0	25.0	41.0
35	34.33153604964878	37.0	33.0	40.0	24.0	41.0
36	34.19737307612327	37.0	33.0	40.0	23.0	41.0
37	34.001712213157035	36.0	33.0	40.0	23.0	41.0
38	33.83292148395408	36.0	33.0	40.0	23.0	41.0
39	33.775317934217526	36.0	33.0	40.0	22.0	41.0
40	33.47451124956452	36.0	32.0	39.0	21.0	41.0
41	33.565912104590836	36.0	33.0	40.0	21.0	41.0
42	33.47103821152044	36.0	32.0	39.0	21.0	41.0
43	33.43693033128894	36.0	32.0	39.0	21.0	41.0
44	33.37069414849803	36.0	32.0	39.0	21.0	40.0
45	33.177515184414	35.0	32.0	39.0	20.0	40.0
46	33.064699511992245	35.0	32.0	39.0	20.0	40.0
47	32.991244487294786	35.0	32.0	39.0	20.0	40.0
48	32.83975143202055	35.0	31.0	39.0	19.0	40.0
49	32.70039375501308	35.0	31.0	39.0	18.0	40.0
50	32.54774887180277	35.0	31.0	39.0	18.0	40.0
51	30.454665510787752	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	10.0
11	14.0
12	17.0
13	21.0
14	45.0
15	97.0
16	242.0
17	560.0
18	1125.0
19	2082.0
20	3356.0
21	4743.0
22	6500.0
23	8114.0
24	9525.0
25	10420.0
26	11517.0
27	13519.0
28	16245.0
29	20034.0
30	24948.0
31	31113.0
32	39719.0
33	52588.0
34	77500.0
35	80841.0
36	75034.0
37	93426.0
38	105015.0
39	52177.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.900375660646915	37.5683602453272	20.371150558629797	23.16011353539609
2	20.44501338172901	37.97507838641464	20.003321801550715	21.576586430305632
3	19.142894180365722	36.14849263127193	21.342169865588566	23.366443322773787
4	20.94044793008553	36.64878835262949	17.627153432123173	24.78361028516181
5	16.813447084781558	41.842006476162695	16.275342240082534	25.06920419897321
6	18.091530486306347	41.0738061094142	21.040237009190317	19.794426395089136
7	75.26810719426598	16.46708850845709	4.365198322355184	3.8996059749217484
8	77.72529511371093	12.56734750095198	3.3779751053929314	6.3293822799441495
9	73.06653595512597	16.801834282612393	5.874862604346428	4.256767157915204
10	37.25157380475908	42.42008096553698	9.669818327162345	10.658526902541583
11	29.102627464007064	31.06546109576241	24.68665689030763	15.145254549922896
12	28.141870633383835	30.628630688585158	26.774530694256526	14.454967983774486
13	22.483195195000555	38.86534820852272	22.30265663104507	16.34879996543166
14	16.308425223006314	40.84303542444792	27.00476124888936	15.843778103656412
15	15.198727452934394	34.97127856951883	31.61355835163025	18.21643562591653
16	17.287681517550187	33.61177052022653	31.73130676432223	17.369241197901054
17	17.38098903265358	32.54933415433144	25.57665664724898	24.493020165765998
18	20.87725268107194	33.1637324086302	25.552350782243753	20.4066641280541
19	20.82972121172839	35.981457325652684	24.92674482352592	18.262076639093014
20	22.63537691644994	36.65864573121494	23.388723699028578	17.317253653306544
21	19.80549906692485	34.8623072747454	24.84194436117435	20.4902492971554
22	19.58026471787642	34.26046705069933	24.58146650786835	21.577801723555893
23	18.598442804248666	35.20461487356899	22.2243377326949	23.972604589487442
24	17.015050731741567	37.15583570315517	26.763322989837445	19.06579057526581
25	16.562691577477644	34.87932138024906	26.200237117216385	22.357749925056915
26	19.5527180708705	38.572867632959834	21.897018750624525	19.97739554554514
27	16.21349731690257	37.08629392272355	26.59048128313362	20.10972747724026
28	18.042918756295894	36.13417917743551	26.238721403474656	19.584180662793933
29	17.581512418946694	34.33149418954794	26.308803314239725	21.778190077265645
30	20.570998782006097	34.67083107153756	25.37964410812329	19.37852603833305
31	22.910303256175716	36.4797275582598	20.090012720069353	20.519956465495124
32	22.519248894758306	33.268517693319396	21.834093566777664	22.378139845144634
33	23.738052992187015	34.45869488307529	21.08088181678239	20.72237030795531
34	18.076136771803036	34.69635222979305	21.889591958539594	25.33791903986432
35	18.37590910686749	34.09586233158061	24.963338653617118	22.564889907934784
36	22.363961423891585	32.62333201001402	24.591593951620528	20.42111261447387
37	19.445637232264147	34.87729589149862	23.93236487964546	21.74470199659178
38	21.45357174686252	35.188275930982144	22.465235861413362	20.89291646074198
39	20.62352645693406	31.36577356116031	24.77442806949317	23.236271912412466
40	22.977279417523448	33.85752982194604	21.961159227721648	21.204031532808866
41	19.099278655939678	29.30193555704992	26.765348478587885	24.833437308422525
42	22.54139423842973	31.451384219012045	22.569481015769107	23.437740526789113
43	21.146102554546413	30.30495758626557	25.870217483478765	22.67872237570926
44	19.618073841217885	31.776272614581895	25.024238348713546	23.58141519548667
45	21.57591126738882	35.586081921567676	23.084900386463254	19.753106424580253
46	20.378307285548004	31.799498218920224	27.619294535771484	20.20289995976029
47	21.53526645979675	32.85180714106314	23.373194951941905	22.239731447198206
48	21.348381364423236	29.45506250658284	28.30147914691815	20.895076982075775
49	21.195929577807124	29.02133784882292	26.796541005344586	22.986191568025365
50	20.154018164583114	31.644210748053503	24.068612756258084	24.133158331105296
51	19.18097336887391	31.758853411328154	26.3243320613264	22.73584115847154
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22168.0
1	17434.5
2	12701.0
3	8397.0
4	4093.0
5	3648.0
6	3203.0
7	3354.5
8	3506.0
9	3715.5
10	3925.0
11	4175.5
12	4426.0
13	4524.0
14	4622.0
15	4729.0
16	4836.0
17	4563.5
18	4291.0
19	4050.5
20	3810.0
21	3835.5
22	3861.0
23	4188.5
24	4516.0
25	5246.5
26	6654.5
27	7332.0
28	8740.0
29	10148.0
30	12242.0
31	14336.0
32	15359.0
33	16382.0
34	19312.0
35	22242.0
36	22403.5
37	22565.0
38	25217.0
39	27869.0
40	31441.5
41	35014.0
42	41308.0
43	47602.0
44	50872.5
45	54143.0
46	61367.5
47	68592.0
48	67376.0
49	66160.0
50	62091.0
51	58022.0
52	53435.0
53	48848.0
54	42974.5
55	37101.0
56	32792.0
57	28483.0
58	25269.5
59	22056.0
60	20743.0
61	19430.0
62	16860.0
63	14290.0
64	12630.0
65	10970.0
66	8995.5
67	7021.0
68	6081.0
69	5141.0
70	4272.0
71	3403.0
72	3113.0
73	2823.0
74	2354.0
75	1680.0
76	1475.0
77	1006.5
78	538.0
79	413.0
80	288.0
81	254.5
82	221.0
83	180.0
84	139.0
85	91.0
86	43.0
87	37.5
88	32.0
89	23.0
90	14.0
91	9.5
92	5.0
93	6.0
94	7.0
95	6.5
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	740562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.134332116828826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31609513030035	23.399557898529892
2	8.535017696055998	4.9732408035981335
3	3.161391657274354	2.7631510348320876
4	1.5573552033351992	1.8149001487135674
5	0.9274325764476337	1.3510064349095798
6	0.5833393007007045	1.0197120554047803
7	0.4067217778628911	0.8294697148783182
8	0.3422414604995502	0.7976781099473957
9	0.24051470540375677	0.6306511775592866
>10	2.6682608122779627	17.95776805911232
>50	0.7613201173157607	15.799071869185518
>100	0.4932297102259729	20.842294352725208
>500	0.0023599507666314493	0.4423734622922846
>1k	0.002831940919957739	2.2646716041316863
>5k	0.0014159704599788696	2.470663350017245
>10k+	4.7199015332628986E-4	2.6437899241627183
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19226	2.596136447724836	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGT	6547	0.8840583232734058	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	6246	0.8434135156813339	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	5174	0.6986585863168783	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4731	0.6388391518873504	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3743	0.5054269595253335	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	3235	0.43683040717725186	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2248	0.3035532473985973	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT	1343	0.18134875945565665	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT	1169	0.1578530899506051	No Hit
AACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	859	0.11599298910827183	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3503258336236535E-4	0.0	0.0	0.6188543295497203	0.0
2	1.3503258336236535E-4	0.0	0.0	0.7414639152427481	0.0
3	1.3503258336236535E-4	0.0	0.0	1.0583853883942196	0.0
4	1.3503258336236535E-4	0.0	0.0	2.619362052063163	0.0
5	1.3503258336236535E-4	0.0	0.0	2.7915285958501785	0.0
6	1.3503258336236535E-4	0.0	0.0	3.589301098355033	0.0
7	1.3503258336236535E-4	0.0	0.0	4.265004145500309	0.0
8	1.3503258336236535E-4	0.0	0.0	4.7243849940990765	0.0
9	1.3503258336236535E-4	0.0	0.0	6.474137209308606	0.0
10	1.3503258336236535E-4	0.0	0.0	7.379935778503353	0.0
11	1.3503258336236535E-4	0.0	0.0	9.237308962652689	0.0
12	1.3503258336236535E-4	0.0	0.0	9.712758688671576	0.0
13	1.3503258336236535E-4	0.0	0.0	9.960813544308241	0.0
14	1.3503258336236535E-4	0.0	0.0	10.269903127624696	0.0
15	1.3503258336236535E-4	0.0	0.0	10.452467180330613	0.0
16	1.3503258336236535E-4	0.0	0.0	10.757235720979473	0.0
17	1.3503258336236535E-4	0.0	0.0	11.13438172631056	0.0
18	1.3503258336236535E-4	0.0	0.0	11.691796230430404	0.0
19	1.3503258336236535E-4	0.0	0.0	11.911089145810884	0.0
20	1.3503258336236535E-4	0.0	0.0	12.18831103945382	0.0
21	1.3503258336236535E-4	0.0	0.0	12.449869153426722	0.0
22	1.3503258336236535E-4	0.0	0.0	12.736138230154936	0.0
23	1.3503258336236535E-4	0.0	0.0	13.03037422930153	0.0
24	2.700651667247307E-4	0.0	0.0	13.235083625678877	0.0
25	2.700651667247307E-4	0.0	0.0	13.414136831217373	0.0
26	2.700651667247307E-4	0.0	0.0	13.587788733421375	0.0
27	2.700651667247307E-4	0.0	0.0	13.788177087131125	0.0
28	2.700651667247307E-4	0.0	0.0	13.98316413750638	0.0
29	2.700651667247307E-4	0.0	0.0	14.195300325968656	0.0
30	2.700651667247307E-4	0.0	0.0	14.48494521728093	0.0
31	2.700651667247307E-4	0.0	0.0	14.703968067494687	0.0
32	2.700651667247307E-4	0.0	0.0	14.91367366945644	0.0
33	2.700651667247307E-4	0.0	0.0	15.115412348999813	0.0
34	2.700651667247307E-4	0.0	0.0	15.332544743046498	0.0
35	2.700651667247307E-4	0.0	0.0	15.574523132431855	0.0
36	2.700651667247307E-4	0.0	0.0	15.79165552647854	0.0
37	2.700651667247307E-4	0.0	0.0	16.01661981036024	0.0
38	2.700651667247307E-4	0.0	0.0	16.24090893132513	0.0
39	2.700651667247307E-4	0.0	0.0	16.46951909495761	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTAC	55	1.8189894E-12	45.000004	22
TCCTATC	30	2.1644519E-6	45.000004	43
ACGTAAG	30	2.1644519E-6	45.000004	1
TTCGCGT	30	2.1644519E-6	45.000004	32
GCGTATT	30	2.1644519E-6	45.000004	12
TCACGGA	20	7.0316595E-4	45.0	3
CGGGTCA	25	3.8894563E-5	45.0	6
CGGGTAT	25	3.8894563E-5	45.0	6
GCCCATA	20	7.0316595E-4	45.0	41
ACACGTA	45	3.8380676E-10	45.0	38
TCGCACG	20	7.0316595E-4	45.0	16
AGATCCG	50	2.1827873E-11	45.0	1
TACTTCG	20	7.0316595E-4	45.0	13
ATCTACG	25	3.8894563E-5	45.0	1
ACGTTAT	35	1.2111377E-7	45.0	32
GCGGTAG	20	7.0316595E-4	45.0	1
TCCGACA	45	3.8380676E-10	45.0	15
TCCGAAC	20	7.0316595E-4	45.0	31
ATTACCG	20	7.0316595E-4	45.0	1
ATAGCCG	25	3.8894563E-5	45.0	1
>>END_MODULE