FastQCFastQC Report
Sat 18 Jun 2016
SRR3548347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821299
Sequences flagged as poor quality0
Sequence length46
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA241972.9461864680219993No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTAT64320.7831496202966276No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT61810.7525882778379128No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGT60520.7368814524308444No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59250.7214181436967536No Hit
AATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGT55030.6700361256984363No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46310.5638628562801123No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28610.34835060069475304No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC14430.17569727955348782No Hit
AACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTC12960.15779880408961902No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC12070.1469623121421066No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTACGCAT10110.1230976781902815No Hit
AAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGT9120.11104360287788004No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGA200.001255559340.0000043
TCCGGAC200.001255559340.0000047
AACGTTA401.9272193E-840.00000431
TGATATC200.001255559340.00000434
CTGTCGG200.001255559340.0000042
TCGTTGA200.001255559340.00000424
TCGCAAC200.001255559340.00000426
CGAAACG200.001255559340.0000041
ACGTTAC200.001255559340.00000433
CTATCGC200.001255559340.00000419
ACCGCAA401.9272193E-840.00000436
CGTGAGC200.001255559340.00000422
CATTGCG200.001255559340.0000041
TTCGTTA200.001255559340.0000046
ATTCGTG200.001255559340.00000422
CATCTCG200.001255559340.00000423
CGATTGT200.001255559340.00000431
TCGATCA401.9272193E-840.00000417
CCGTCGT200.001255559340.00000411
ATCGTGT200.001255559340.00000414
Produced by FastQC (version 0.11.5)