##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821299
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.881627762848854	31.0	31.0	33.0	28.0	34.0
2	31.197431142616757	31.0	31.0	34.0	28.0	34.0
3	31.38068109178265	31.0	31.0	34.0	28.0	34.0
4	35.36233332805714	37.0	35.0	37.0	33.0	37.0
5	34.90498101178743	35.0	35.0	37.0	32.0	37.0
6	35.04901868868707	35.0	35.0	37.0	32.0	37.0
7	34.950059600705714	36.0	35.0	37.0	32.0	37.0
8	35.2790688896492	37.0	35.0	37.0	33.0	37.0
9	36.671896593079985	39.0	35.0	39.0	32.0	39.0
10	35.88237414145153	37.0	35.0	39.0	31.0	39.0
11	36.144494270661475	37.0	35.0	39.0	31.0	39.0
12	35.864212667980844	37.0	35.0	39.0	31.0	39.0
13	35.543910317679675	37.0	35.0	39.0	30.0	39.0
14	36.64646127658745	38.0	35.0	40.0	31.0	41.0
15	36.68317872053905	38.0	35.0	40.0	31.0	41.0
16	36.51327348505234	38.0	35.0	40.0	31.0	41.0
17	36.52092356133394	38.0	35.0	40.0	31.0	41.0
18	36.3088850710886	38.0	35.0	40.0	31.0	41.0
19	36.410032156376666	38.0	35.0	40.0	31.0	41.0
20	36.18926481098845	38.0	34.0	40.0	30.0	41.0
21	36.31589835127036	38.0	34.0	40.0	31.0	41.0
22	36.35259144355466	38.0	35.0	40.0	31.0	41.0
23	36.17069544709052	38.0	35.0	40.0	30.0	41.0
24	35.77014704754298	38.0	34.0	40.0	29.0	41.0
25	35.94975398728113	38.0	34.0	40.0	30.0	41.0
26	35.949551868442555	38.0	34.0	40.0	30.0	41.0
27	35.832374080572365	38.0	34.0	40.0	30.0	41.0
28	35.78799681967225	38.0	34.0	40.0	30.0	41.0
29	35.54792347245035	38.0	34.0	40.0	29.0	41.0
30	35.55495258121586	38.0	34.0	40.0	29.0	41.0
31	35.40023304545604	37.0	34.0	40.0	28.0	41.0
32	35.360897797269935	38.0	34.0	40.0	28.0	41.0
33	35.30599696334709	38.0	34.0	40.0	27.0	41.0
34	35.28790002179474	38.0	34.0	40.0	27.0	41.0
35	35.04235850768112	38.0	34.0	40.0	26.0	41.0
36	35.03416782438551	38.0	34.0	40.0	26.0	41.0
37	34.8673942133133	37.0	34.0	40.0	25.0	41.0
38	34.63767884777651	37.0	33.0	40.0	24.0	41.0
39	34.64017489367453	37.0	33.0	40.0	24.0	41.0
40	34.36940139949032	37.0	33.0	40.0	23.0	41.0
41	34.51795387550697	37.0	33.0	40.0	24.0	41.0
42	34.3808771227044	37.0	33.0	40.0	23.0	41.0
43	34.357232871341616	37.0	33.0	40.0	23.0	41.0
44	34.27281416390377	37.0	33.0	40.0	23.0	41.0
45	34.0847291911959	36.0	33.0	40.0	23.0	41.0
46	33.022668967087505	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	2.0
9	5.0
10	10.0
11	11.0
12	10.0
13	18.0
14	34.0
15	46.0
16	99.0
17	236.0
18	460.0
19	967.0
20	1737.0
21	3041.0
22	4631.0
23	6273.0
24	7792.0
25	9251.0
26	10786.0
27	11900.0
28	13750.0
29	16933.0
30	21637.0
31	28309.0
32	37312.0
33	51655.0
34	81218.0
35	93305.0
36	82816.0
37	108714.0
38	141726.0
39	86614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.546595088999258	36.84906471333826	20.40961939561597	24.194720802046515
2	20.92709232569381	36.96303051629187	19.31756887564699	22.792308282367323
3	18.993448183913532	35.8348177703857	21.293219643515943	23.87851440218483
4	20.694290386327026	36.39563666825359	17.802651653052052	25.107421292367338
5	16.92806152205226	41.13337529937331	16.547323203851462	25.391239974722968
6	18.32743008331923	40.2556194516248	21.241107075498693	20.175843389557276
7	76.34625148697369	14.775495891264937	4.662004945823628	4.216247675937752
8	77.9754997875317	11.670658310797895	3.759288639094897	6.594553262575506
9	74.1163693125159	15.01596860583052	6.628158563446443	4.239503518207133
10	37.04071233497179	42.11986134160641	9.669803567275743	11.169622756146056
11	28.039970826702575	30.72462038794641	25.4742791602084	15.761129625142608
12	28.071019202507248	30.08745901309998	27.114972744396376	14.726549039996396
13	22.345455187453048	38.52385063174313	22.71693987208069	16.41375430872313
14	16.131883759751318	40.18621719982613	27.01914893357961	16.662750106842942
15	14.854760568319211	34.33926012329249	31.827020366516944	18.97895894187135
16	16.907606121522125	34.24112290408243	31.12837103174362	17.722899942651825
17	16.958622864510975	32.18559866747677	25.86634100370267	24.98943746430959
18	20.57399314987599	32.39624058960257	25.657403698287713	21.37236256223373
19	21.0215767948092	35.88924374679623	25.126659109532586	17.962520348861986
20	22.202267383742097	36.42875493577857	23.75870419907975	17.610273481399588
21	19.621355925186805	34.94342498895043	25.48401982712751	19.951199258735247
22	19.39561596933638	34.031211531975565	24.783422358970363	21.789750139717693
23	18.376376934587768	35.46589001082431	22.349838487566647	23.80789456702127
24	16.471589518555362	36.75214507749309	26.907253022346307	19.869012381605238
25	16.378200874468373	34.78964420996495	26.435682985124785	22.396471930441898
26	19.59237744110245	37.855884397765	22.353491237661316	20.198246923471235
27	15.759668525104741	37.087832811193	26.416932201305492	20.735566462396765
28	17.74786040163205	35.72255658414292	26.831154062040742	19.698428952184283
29	17.46672040267917	34.39551247475037	26.065659400535008	22.072107722035458
30	20.1755998728843	34.30419372238369	25.472939818507022	20.047266586224993
31	22.64278904515895	36.025491325327316	20.234530907744926	21.09718872176881
32	22.57594371842654	33.041438014657274	21.871084708492276	22.51153355842391
33	22.85598789235102	34.15503976018478	21.52991784965037	21.459054497813828
34	17.910651297517713	34.17038131058238	22.18765638336343	25.73131100853648
35	18.325847224944873	34.08600278339557	24.665316772576126	22.922833219083426
36	21.702814687464613	32.21640352660845	25.02255573183457	21.05822605409236
37	18.97140992500904	34.847479419797175	23.885089352355234	22.296021302838554
38	20.92295254225319	35.02110680763035	23.008185812962147	21.04775483715431
39	20.53697861558336	31.031451395898447	24.743120349592534	23.688449638925654
40	22.786707398888833	33.154551509255455	21.92514540989335	22.133595681962355
41	18.945597157673394	29.094519779032975	26.58386288063178	25.376020182661858
42	22.40694314737994	31.261331135189497	22.849656458853595	23.482069258576963
43	20.574236666548966	30.195823932575106	26.182060370218394	23.04787903065753
44	19.638280333958765	31.229795726038873	25.094271392026535	24.03765254797583
45	21.395740162839598	34.80644686040041	23.755173207321572	20.042639769438413
46	20.00197248505112	31.47014668226797	27.893495547906426	20.634385284774485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28140.0
1	21974.0
2	15808.0
3	10639.0
4	5470.0
5	4908.5
6	4347.0
7	4347.0
8	4394.5
9	4442.0
10	4454.5
11	4467.0
12	4554.5
13	4642.0
14	4615.5
15	4589.0
16	4635.5
17	4682.0
18	4666.5
19	4651.0
20	4651.0
21	4467.5
22	4284.0
23	4918.0
24	5552.0
25	6559.0
26	7566.0
27	8646.0
28	9726.0
29	10824.0
30	11922.0
31	11922.0
32	14026.0
33	16130.0
34	19325.5
35	22521.0
36	25628.5
37	28736.0
38	30500.5
39	32265.0
40	35956.0
41	39647.0
42	49257.0
43	58867.0
44	58867.0
45	62801.5
46	66736.0
47	77569.5
48	88403.0
49	86529.5
50	84656.0
51	76178.0
52	67700.0
53	58266.0
54	48832.0
55	43173.0
56	37514.0
57	37514.0
58	33412.5
59	29311.0
60	27076.5
61	24842.0
62	21042.5
63	17243.0
64	15528.0
65	13813.0
66	11073.5
67	8334.0
68	6895.5
69	5457.0
70	5457.0
71	4860.5
72	4264.0
73	3419.0
74	2574.0
75	1938.5
76	1303.0
77	1020.0
78	737.0
79	764.0
80	791.0
81	791.0
82	521.0
83	251.0
84	149.5
85	48.0
86	34.0
87	20.0
88	12.0
89	4.0
90	5.0
91	6.0
92	3.5
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	821299.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.986102097338733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36866192419	18.317865219507663
2	9.758814922759946	4.681518621727056
3	3.7774544838746267	2.7181922675480035
4	1.8558724270276656	1.7806058205728565
5	1.1445520220858174	1.3726670828732956
6	0.8017142075924938	1.1537999301720345
7	0.5598585195419179	0.9400176526858148
8	0.4338277872119982	0.8324670077383625
9	0.34285413681103694	0.7401360897040026
>10	3.337662167176312	18.138645293038362
>50	0.9255708589244469	15.80755962719414
>100	0.6839621302978716	24.828916349147946
>500	0.0030648041686237707	0.5106682622415427
>1k	0.0030648041686237707	1.5252661220357402
>5k	0.0025540034738531425	3.6870297542309847
>10k+	5.108006947706285E-4	2.9646448995821997
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24197	2.9461864680219993	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTAT	6432	0.7831496202966276	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT	6181	0.7525882778379128	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGT	6052	0.7368814524308444	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5925	0.7214181436967536	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGT	5503	0.6700361256984363	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4631	0.5638628562801123	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2861	0.34835060069475304	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	1443	0.17569727955348782	No Hit
AACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTC	1296	0.15779880408961902	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC	1207	0.1469623121421066	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTACGCAT	1011	0.1230976781902815	No Hit
AAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGT	912	0.11104360287788004	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.435166729778071E-4	0.0	0.0	0.8507254970479692	0.0
2	2.435166729778071E-4	0.0	0.0	1.040303226961192	0.0
3	2.435166729778071E-4	0.0	0.0	1.4310257287540835	0.0
4	3.6527500946671065E-4	0.0	0.0	3.076346129728637	0.0
5	3.6527500946671065E-4	0.0	0.0	3.2804131016840397	0.0
6	3.6527500946671065E-4	0.0	0.0	4.270430135675315	0.0
7	3.6527500946671065E-4	0.0	0.0	5.067460206331677	0.0
8	3.6527500946671065E-4	0.0	0.0	5.661640888397526	0.0
9	3.6527500946671065E-4	0.0	0.0	7.281148522036433	0.0
10	3.6527500946671065E-4	0.0	0.0	8.431764801856572	0.0
11	3.6527500946671065E-4	0.0	0.0	10.308426042160042	0.0
12	3.6527500946671065E-4	0.0	0.0	10.915634866230203	0.0
13	3.6527500946671065E-4	0.0	0.0	11.210046523860372	0.0
14	3.6527500946671065E-4	0.0	0.0	11.564241524706594	0.0
15	3.6527500946671065E-4	0.0	0.0	11.774274655149952	0.0
16	3.6527500946671065E-4	0.0	0.0	12.15744814008053	0.0
17	3.6527500946671065E-4	0.0	0.0	12.632792685733211	0.0
18	3.6527500946671065E-4	0.0	0.0	13.390738330376635	0.0
19	3.6527500946671065E-4	0.0	0.0	13.68563702135276	0.0
20	3.6527500946671065E-4	0.0	0.0	14.009148921403776	0.0
21	3.6527500946671065E-4	0.0	0.0	14.346663030151017	0.0
22	3.6527500946671065E-4	0.0	0.0	14.694891872509281	0.0
23	3.6527500946671065E-4	0.0	0.0	15.079526457477728	0.0
24	7.305500189334213E-4	0.0	0.0	15.328765772270513	0.0
25	7.305500189334213E-4	0.0	0.0	15.550731219689784	0.0
26	7.305500189334213E-4	0.0	0.0	15.757111600038476	0.0
27	7.305500189334213E-4	0.0	0.0	16.00647267316775	0.0
28	7.305500189334213E-4	0.0	0.0	16.23598713744933	0.0
29	7.305500189334213E-4	0.0	0.0	16.484617660559675	0.0
30	7.305500189334213E-4	0.0	0.0	16.86572125376994	0.0
31	7.305500189334213E-4	0.0	0.0	17.120683210377706	0.0
32	7.305500189334213E-4	0.0	0.0	17.368583183469113	0.0
33	7.305500189334213E-4	0.0	0.0	17.6167266732335	0.0
34	7.305500189334213E-4	0.0	0.0	17.86547895468033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	20	0.0012555593	40.000004	3
TCCGGAC	20	0.0012555593	40.000004	7
AACGTTA	40	1.9272193E-8	40.000004	31
TGATATC	20	0.0012555593	40.000004	34
CTGTCGG	20	0.0012555593	40.000004	2
TCGTTGA	20	0.0012555593	40.000004	24
TCGCAAC	20	0.0012555593	40.000004	26
CGAAACG	20	0.0012555593	40.000004	1
ACGTTAC	20	0.0012555593	40.000004	33
CTATCGC	20	0.0012555593	40.000004	19
ACCGCAA	40	1.9272193E-8	40.000004	36
CGTGAGC	20	0.0012555593	40.000004	22
CATTGCG	20	0.0012555593	40.000004	1
TTCGTTA	20	0.0012555593	40.000004	6
ATTCGTG	20	0.0012555593	40.000004	22
CATCTCG	20	0.0012555593	40.000004	23
CGATTGT	20	0.0012555593	40.000004	31
TCGATCA	40	1.9272193E-8	40.000004	17
CCGTCGT	20	0.0012555593	40.000004	11
ATCGTGT	20	0.0012555593	40.000004	14
>>END_MODULE