##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086879
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.139429504112233	31.0	31.0	34.0	28.0	34.0
2	31.317059212663047	31.0	31.0	34.0	28.0	34.0
3	31.51632886457462	31.0	31.0	34.0	30.0	34.0
4	35.44689243236828	37.0	35.0	37.0	33.0	37.0
5	35.03140275964482	37.0	35.0	37.0	32.0	37.0
6	35.02964727444361	36.0	35.0	37.0	32.0	37.0
7	34.32582927814412	37.0	35.0	37.0	32.0	37.0
8	34.64937495342168	37.0	35.0	37.0	32.0	37.0
9	35.772188072453325	39.0	35.0	39.0	31.0	39.0
10	35.32757004229541	37.0	35.0	39.0	30.0	39.0
11	35.833927235690446	37.0	35.0	39.0	31.0	39.0
12	35.83334667428481	37.0	35.0	39.0	31.0	39.0
13	35.7747329739557	37.0	35.0	39.0	30.0	39.0
14	36.834904345377915	39.0	35.0	40.0	31.0	41.0
15	36.885311980450446	38.0	35.0	40.0	31.0	41.0
16	36.798398901809676	38.0	35.0	40.0	31.0	41.0
17	36.740907681535845	38.0	35.0	40.0	31.0	41.0
18	36.607407080272964	38.0	35.0	40.0	31.0	41.0
19	36.63624837723427	38.0	35.0	40.0	31.0	41.0
20	36.57296350375709	38.0	35.0	40.0	31.0	41.0
21	36.59719895222927	38.0	35.0	40.0	31.0	41.0
22	36.59262806623369	38.0	35.0	40.0	31.0	41.0
23	36.51509505657943	38.0	35.0	40.0	31.0	41.0
24	36.163479099329365	38.0	35.0	40.0	30.0	41.0
25	36.2036878070144	38.0	35.0	40.0	30.0	41.0
26	36.11371274999333	38.0	35.0	40.0	30.0	41.0
27	35.9790813880846	38.0	34.0	40.0	30.0	41.0
28	35.90928521022119	38.0	34.0	40.0	29.0	41.0
29	35.69404505929363	38.0	34.0	40.0	29.0	41.0
30	35.724141325759355	38.0	34.0	40.0	29.0	41.0
31	35.61674114597853	38.0	34.0	40.0	29.0	41.0
32	35.56960710437868	38.0	34.0	40.0	28.0	41.0
33	35.53436123064297	38.0	34.0	40.0	28.0	41.0
34	35.488407633232406	38.0	34.0	40.0	28.0	41.0
35	35.37657549736447	38.0	34.0	40.0	27.0	41.0
36	35.300569796637895	38.0	34.0	40.0	27.0	41.0
37	35.22913406184129	38.0	34.0	40.0	27.0	41.0
38	35.13810092935828	38.0	34.0	40.0	27.0	41.0
39	35.03814408043581	38.0	34.0	40.0	26.0	41.0
40	34.84638860443527	37.0	33.0	40.0	25.0	41.0
41	34.844872336295026	37.0	33.0	40.0	26.0	41.0
42	34.780340773903994	37.0	33.0	40.0	25.0	41.0
43	34.75994199906337	37.0	33.0	40.0	26.0	41.0
44	34.70528366083069	37.0	33.0	40.0	25.0	41.0
45	34.54139421223522	37.0	33.0	40.0	25.0	41.0
46	34.432721581703206	37.0	33.0	40.0	24.0	41.0
47	34.309764012369364	37.0	33.0	40.0	24.0	41.0
48	34.186469699018936	36.0	33.0	40.0	24.0	41.0
49	33.98702891490221	36.0	33.0	40.0	23.0	41.0
50	33.85072487369799	36.0	33.0	39.0	23.0	40.0
51	31.83792584087097	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	24.0
11	29.0
12	30.0
13	47.0
14	91.0
15	172.0
16	278.0
17	540.0
18	1033.0
19	1863.0
20	2959.0
21	4356.0
22	5699.0
23	7405.0
24	8985.0
25	10476.0
26	12569.0
27	15203.0
28	18877.0
29	23714.0
30	30520.0
31	39476.0
32	50949.0
33	68256.0
34	98334.0
35	107876.0
36	120855.0
37	160372.0
38	192863.0
39	103019.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.56468199311975	33.01600270131266	20.604225493362186	24.815089812205407
2	23.871194493591283	31.88827827200636	20.17924718390916	24.0612800504932
3	21.330709306187718	31.5723277384143	20.618578517019834	26.478384438378143
4	20.63900397376341	33.27702531744564	18.524417161431952	27.559553547359002
5	17.370930894791417	36.86454517936219	17.676760706573592	28.087763219272798
6	19.89715506509924	38.23645502397231	19.981985115178414	21.884404795750033
7	83.06766438582399	9.764932434981263	3.800883078981193	3.366520100213547
8	85.57097892221674	7.287103716237042	2.9532266241228324	4.188690737423393
9	79.23283088549876	11.804349886233886	5.254218730879886	3.7086004973874735
10	36.159038862651684	38.74120302259957	11.262431236595793	13.83732687815295
11	28.85040561092817	29.54146689741912	24.66493510317156	16.943192388481148
12	28.399941483826623	26.87566877269687	27.512722207347828	17.211667536128676
13	24.420565674743923	32.00623068437241	24.144914015267567	19.4282896256161
14	18.84920032496718	34.29903420711965	28.312167223766398	18.539598244146774
15	19.089153438423228	30.083845579866757	30.764970157671645	20.06203082403837
16	21.545268608557162	29.39425639836633	29.788320503018273	19.27215449005823
17	20.62382289104859	28.414202500922364	26.597900962296634	24.364073645732415
18	22.44251660028393	29.090358724384224	26.42741280308112	22.039711872250727
19	23.285940753294526	31.299436275795188	25.174835469265666	20.239787501644617
20	26.305872134800655	30.77435482698626	23.92603040448845	18.993742633724636
21	22.892612701137846	30.52520105733941	25.65566176179685	20.926524479725895
22	23.080398093992063	29.009116930219463	24.862749211273748	23.047735764514726
23	21.966106622724332	31.160138341066485	24.358737265141748	22.515017771067434
24	20.144836729755568	30.790824001567792	27.791502089929054	21.27283717874759
25	21.29123849112919	30.93509029063953	25.267394070545112	22.506277147686173
26	22.318951787641495	33.35348277039118	22.840352973974102	21.487212467993217
27	20.248804144711603	31.586772768633857	25.735339444409178	22.429083642245367
28	20.300419825941987	32.07192336957472	26.99582934254871	20.631827461934584
29	20.50679054430162	31.005843336746775	26.333198083687325	22.154168035264274
30	22.823055740335402	29.492795426169792	26.389690112698837	21.294458720795966
31	24.27372320193876	30.65014596841047	23.146274792318188	21.92985603733258
32	23.91903790578344	29.335648218430936	25.369153328015354	21.37616054777027
33	23.322191338686274	30.32729494267531	24.315954213854532	22.034559504783882
34	21.434584714581845	30.430618311697987	25.80029607711622	22.334500896603945
35	22.14432333314012	29.778935833703663	27.679622110648932	20.39711872250729
36	24.484694248393797	29.983282407701317	25.51305159083946	20.01897175306543
37	22.753222759847233	30.13684135952576	24.616907677855586	22.493028202771423
38	22.464874194827576	29.210427287674158	25.899663163976854	22.425035353521412
39	22.50839329861006	28.245738486068827	26.29510736705742	22.950760848263698
40	24.17923246285925	28.703471131561102	25.61987120921464	21.497425196365004
41	21.14835230048607	27.383544994429005	28.452201210990367	23.01590149409456
42	24.336931709969555	28.030627144327937	25.234455721382048	22.397985424320463
43	22.203759572132682	28.079298615577265	26.529540086798992	23.18740172549106
44	22.904205527938252	28.712763794313812	25.401447631245063	22.981583046502877
45	22.716328128522125	29.007092785857484	25.561079016155432	22.715500069464955
46	21.842173783834262	28.741101815381477	27.304879383997665	22.111845016786596
47	22.234581770371864	29.55885613761973	26.092508917736012	22.11405317427239
48	22.989679623950778	27.398450057458096	27.434424623164123	22.177445695426997
49	22.430003707864447	27.523210955405343	27.792330148986228	22.25445518774399
50	20.655289135221125	29.336292264364296	26.27661404811391	23.73180455230067
51	20.235003160425403	28.265979929688584	27.646867774609685	23.852149135276328
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13342.0
1	10368.0
2	7394.0
3	4932.0
4	2470.0
5	2202.0
6	1934.0
7	2133.0
8	2332.0
9	2444.0
10	2556.0
11	2747.5
12	2939.0
13	3163.0
14	3387.0
15	3778.5
16	4170.0
17	3949.5
18	3729.0
19	3801.0
20	3873.0
21	4285.5
22	4698.0
23	5042.5
24	5387.0
25	6608.0
26	10377.0
27	12925.0
28	13623.5
29	14322.0
30	18317.5
31	22313.0
32	25321.0
33	28329.0
34	30386.0
35	32443.0
36	37420.0
37	42397.0
38	44111.0
39	45825.0
40	49917.5
41	54010.0
42	58563.5
43	63117.0
44	71727.0
45	80337.0
46	82379.0
47	84421.0
48	83879.0
49	83337.0
50	78602.0
51	73867.0
52	71522.5
53	69178.0
54	63735.0
55	58292.0
56	54697.5
57	51103.0
58	47249.5
59	43396.0
60	40459.0
61	37522.0
62	35992.0
63	34462.0
64	30198.5
65	25935.0
66	22650.5
67	19366.0
68	16733.0
69	14100.0
70	12123.0
71	10146.0
72	8695.5
73	7245.0
74	6522.0
75	4189.0
76	2579.0
77	2283.5
78	1988.0
79	1380.5
80	773.0
81	678.0
82	583.0
83	409.5
84	236.0
85	199.5
86	163.0
87	142.0
88	121.0
89	113.5
90	106.0
91	65.0
92	24.0
93	22.5
94	21.0
95	28.5
96	36.0
97	23.5
98	11.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1086879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.694944130804306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.71876251114863	19.439554422588678
2	8.982595912342747	4.436494084097905
3	3.3112065731522256	2.4531018398863855
4	1.576150372170303	1.5569178152996819
5	0.8907420239470014	1.0998412258165375
6	0.5546466946813087	0.8218181482494114
7	0.3815789824170459	0.6596150156594675
8	0.30461386213855907	0.6017937845584194
9	0.2713673373277735	0.6031261110811044
>10	3.051768465446392	18.343977196977004
>50	1.2490585675637342	22.155910522404152
>100	0.7010526333270758	24.102670800907582
>500	0.003038147923410946	0.5169352943529942
>1k	0.003038147923410946	2.1474572514685892
>5k	0.0	0.0
>10k+	3.7976849042636824E-4	1.0607864866521621
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11311	1.0406862217413346	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGT	4715	0.43381093939619775	No Hit
AATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	3800	0.3496249352503821	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	3685	0.3390441806309626	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	3247	0.29874530651526066	Illumina Single End Adapter 2 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2744	0.252466005875539	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2106	0.19376581937823806	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1431	0.13166139009034125	No Hit
AATGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	1170	0.10764767743235448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3870716059469361	0.0
2	0.0	0.0	0.0	0.4734657675785437	0.0
3	0.0	0.0	0.0	0.6514064583086066	0.0
4	0.0	0.0	0.0	1.5840769763699547	0.0
5	0.0	0.0	0.0	1.71601438614602	0.0
6	0.0	0.0	0.0	2.3040283233000176	0.0
7	0.0	0.0	0.0	2.7231182127909364	0.0
8	0.0	0.0	0.0	2.9927894457432704	0.0
9	0.0	0.0	0.0	3.875224381002853	0.0
10	0.0	0.0	0.0	4.568125798731965	0.0
11	0.0	0.0	0.0	5.50006026429805	0.0
12	0.0	0.0	0.0	5.838276385871841	0.0
13	0.0	0.0	0.0	6.007568459782552	0.0
14	0.0	0.0	0.0	6.183853032398271	0.0
15	0.0	0.0	0.0	6.3119261665742	0.0
16	0.0	0.0	0.0	6.5914421016506894	0.0
17	0.0	0.0	0.0	6.89800796592813	0.0
18	0.0	0.0	0.0	7.350496237391651	0.0
19	0.0	0.0	0.0	7.502583084225567	0.0
20	0.0	0.0	0.0	7.723858865614296	0.0
21	0.0	0.0	0.0	7.925629255878529	0.0
22	0.0	0.0	0.0	8.147641089762521	0.0
23	0.0	0.0	0.0	8.372689140189479	0.0
24	0.0	0.0	0.0	8.539405030366765	0.0
25	0.0	0.0	0.0	8.678610958533563	0.0
26	0.0	0.0	0.0	8.816160768586016	0.0
27	9.200656190799527E-5	0.0	0.0	8.959414985476764	0.0
28	9.200656190799527E-5	0.0	0.0	9.104233313919949	0.0
29	9.200656190799527E-5	0.0	0.0	9.252639898277545	0.0
30	9.200656190799527E-5	0.0	0.0	9.458642590389546	0.0
31	1.8401312381599054E-4	0.0	0.0	9.625266474004926	0.0
32	1.8401312381599054E-4	0.0	0.0	9.78618595078201	0.0
33	1.8401312381599054E-4	0.0	0.0	9.94406921101613	0.0
34	1.8401312381599054E-4	0.0	0.0	10.101400431878801	0.0
35	1.8401312381599054E-4	0.0	0.0	10.275108820761096	0.0
36	1.8401312381599054E-4	0.0	0.0	10.426735634785473	0.0
37	1.8401312381599054E-4	0.0	0.0	10.576706330695504	0.0
38	1.8401312381599054E-4	0.0	0.0	10.737901827158312	0.0
39	1.8401312381599054E-4	0.0	0.0	10.920166826298052	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	35	1.2118471E-7	45.000004	1
GTAACGT	35	1.2118471E-7	45.000004	21
TTAGGCG	55	1.8189894E-12	45.0	1
TCGTTAC	20	7.033117E-4	45.0	43
TCCGCTA	20	7.033117E-4	45.0	37
GTCGATC	20	7.033117E-4	45.0	10
CGACGTC	20	7.033117E-4	45.0	27
CGACACG	40	6.8139343E-9	45.0	1
TACGCCC	25	3.8906655E-5	45.0	13
TCTACGG	25	3.8906655E-5	45.0	2
TCCCGTA	20	7.033117E-4	45.0	35
TTACGTA	20	7.033117E-4	45.0	20
CGTAACG	20	7.033117E-4	45.0	1
CAATGCG	20	7.033117E-4	45.0	1
TCGAGTA	20	7.033117E-4	45.0	37
ACGTAAC	20	7.033117E-4	45.0	38
TCATACG	20	7.033117E-4	45.0	1
ATCGGTC	20	7.033117E-4	45.0	36
GCGCTAT	20	7.033117E-4	45.0	22
TGTACGA	25	3.8906655E-5	45.0	11
>>END_MODULE