FastQCFastQC Report
Sat 18 Jun 2016
SRR3548344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670325
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134302.0035057621303096No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGT46100.6877261030097341No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC41270.6156715026293216No Hit
AATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT36060.5379480102935144RNA PCR Primer, Index 27 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31600.4714131205012494No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT28220.42098981837168536RNA PCR Primer, Index 38 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27760.4141274754783128No Hit
TAGTTCGGGCGAATTGATTTTTTTAGTTATTTTTTATCATTTTCTTCAATG19700.29388729347704473No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14220.21213590422556222No Hit
AATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT10770.1606683325252676No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT8570.12784843173087682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTT406.8084773E-945.00000428
AACCGCA207.031179E-445.0000041
GTCGCCC207.031179E-445.00000431
TCCGCAA207.031179E-445.00000431
CACGGAT207.031179E-445.0000044
GCGAATT2500.045.0000049
GCGAATG253.8890586E-545.0000041
TAGACCG207.031179E-445.0000041
TGGACCG207.031179E-445.00000445
TAGCGTA207.031179E-445.00000423
GGCGTGA207.031179E-445.00000431
CGCATAC207.031179E-445.00000433
CACGATT253.8890586E-545.00000436
CGATTCC207.031179E-445.00000438
CGATTAC253.8890586E-545.00000438
TCGATCT207.031179E-445.00000439
CTGCGGT253.8890586E-545.00000427
TTCCCGC207.031179E-445.00000423
ACTAGCG253.8890586E-545.00000443
CCCGTAG207.031179E-445.0000041