Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548344_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 670325 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 13430 | 2.0035057621303096 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGT | 4610 | 0.6877261030097341 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC | 4127 | 0.6156715026293216 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT | 3606 | 0.5379480102935144 | RNA PCR Primer, Index 27 (95% over 21bp) |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3160 | 0.4714131205012494 | No Hit |
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT | 2822 | 0.42098981837168536 | RNA PCR Primer, Index 38 (95% over 24bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2776 | 0.4141274754783128 | No Hit |
TAGTTCGGGCGAATTGATTTTTTTAGTTATTTTTTATCATTTTCTTCAATG | 1970 | 0.29388729347704473 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1422 | 0.21213590422556222 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT | 1077 | 0.1606683325252676 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT | 857 | 0.12784843173087682 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCTT | 40 | 6.8084773E-9 | 45.000004 | 28 |
AACCGCA | 20 | 7.031179E-4 | 45.000004 | 1 |
GTCGCCC | 20 | 7.031179E-4 | 45.000004 | 31 |
TCCGCAA | 20 | 7.031179E-4 | 45.000004 | 31 |
CACGGAT | 20 | 7.031179E-4 | 45.000004 | 4 |
GCGAATT | 250 | 0.0 | 45.000004 | 9 |
GCGAATG | 25 | 3.8890586E-5 | 45.000004 | 1 |
TAGACCG | 20 | 7.031179E-4 | 45.000004 | 1 |
TGGACCG | 20 | 7.031179E-4 | 45.000004 | 45 |
TAGCGTA | 20 | 7.031179E-4 | 45.000004 | 23 |
GGCGTGA | 20 | 7.031179E-4 | 45.000004 | 31 |
CGCATAC | 20 | 7.031179E-4 | 45.000004 | 33 |
CACGATT | 25 | 3.8890586E-5 | 45.000004 | 36 |
CGATTCC | 20 | 7.031179E-4 | 45.000004 | 38 |
CGATTAC | 25 | 3.8890586E-5 | 45.000004 | 38 |
TCGATCT | 20 | 7.031179E-4 | 45.000004 | 39 |
CTGCGGT | 25 | 3.8890586E-5 | 45.000004 | 27 |
TTCCCGC | 20 | 7.031179E-4 | 45.000004 | 23 |
ACTAGCG | 25 | 3.8890586E-5 | 45.000004 | 43 |
CCCGTAG | 20 | 7.031179E-4 | 45.000004 | 1 |