FastQCFastQC Report
Sat 18 Jun 2016
SRR3548340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577494
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92331.5988044897436164No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22520.38996076149708914No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18900.3272761275441823No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT13260.2296127751976644No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12630.21870357094619167No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT12360.2140281976955605No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC10230.17714469760724785No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT10190.17645204971826547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAG502.1827873E-1145.0000041
CTACTCG253.8883856E-545.00000414
GATCGAA253.8883856E-545.00000429
ACGCATT253.8883856E-545.00000416
CGGTTAG253.8883856E-545.0000041
GCACGGC502.1827873E-1145.00000415
ATGTCGC253.8883856E-545.00000425
TGTGCGA253.8883856E-545.00000430
CGTTCAT453.8380676E-1045.00000417
TACGCAC253.8883856E-545.00000433
TGCGATC253.8883856E-545.00000422
CTACGAC253.8883856E-545.00000423
CACTACC502.1827873E-1145.00000418
TACGACA253.8883856E-545.00000424
CATACCG453.8380676E-1045.0000041
AGTTATC253.8883856E-545.00000416
TATAGCG253.8883856E-545.0000041
TATGTCG253.8883856E-545.00000424
CCCGCTA253.8883856E-545.00000422
ATCCTAC207.030369E-445.030