##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577494
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19396911483063	31.0	31.0	34.0	30.0	34.0
2	31.368308588487498	31.0	31.0	34.0	30.0	34.0
3	31.553565924494453	31.0	31.0	34.0	30.0	34.0
4	35.50162425929966	37.0	35.0	37.0	33.0	37.0
5	35.06862582122066	37.0	35.0	37.0	32.0	37.0
6	35.051169362798575	36.0	35.0	37.0	32.0	37.0
7	34.224445621945854	36.0	35.0	37.0	31.0	37.0
8	34.585800025627975	37.0	35.0	37.0	32.0	37.0
9	35.72104991567012	39.0	35.0	39.0	31.0	39.0
10	35.463078404277795	37.0	35.0	39.0	30.0	39.0
11	35.83938014940415	37.0	35.0	39.0	30.0	39.0
12	35.63513560314046	37.0	35.0	39.0	30.0	39.0
13	35.42117493861408	37.0	35.0	39.0	30.0	39.0
14	36.492820704630695	38.0	35.0	40.0	31.0	41.0
15	36.643513179357704	38.0	35.0	40.0	31.0	41.0
16	36.63383342510918	38.0	35.0	40.0	31.0	41.0
17	36.57578260553356	38.0	35.0	40.0	31.0	41.0
18	36.39434002777518	38.0	35.0	40.0	31.0	41.0
19	36.31576085638985	38.0	35.0	40.0	30.0	41.0
20	36.19770075533252	38.0	34.0	40.0	30.0	41.0
21	36.22901363477369	38.0	34.0	40.0	30.0	41.0
22	36.173823450979576	38.0	34.0	40.0	30.0	41.0
23	36.10323397299366	38.0	34.0	40.0	30.0	41.0
24	35.72941363892958	38.0	34.0	40.0	29.0	41.0
25	35.7373808212726	38.0	34.0	40.0	29.0	41.0
26	35.63375896546111	38.0	34.0	40.0	29.0	41.0
27	35.470862034930235	38.0	34.0	40.0	28.0	41.0
28	35.349503198301626	37.0	34.0	40.0	27.0	41.0
29	35.123443706774445	37.0	34.0	40.0	27.0	41.0
30	35.16785282617655	37.0	34.0	40.0	27.0	41.0
31	34.969424790560595	37.0	33.0	40.0	26.0	41.0
32	34.845350774207176	37.0	33.0	40.0	26.0	41.0
33	34.76917855423606	37.0	33.0	40.0	25.0	41.0
34	34.653930603608	37.0	33.0	40.0	25.0	41.0
35	34.49752551541661	37.0	33.0	40.0	24.0	41.0
36	34.318893356467775	37.0	33.0	40.0	24.0	41.0
37	34.19148250890918	37.0	33.0	40.0	23.0	41.0
38	34.06741022417549	36.0	33.0	40.0	23.0	41.0
39	33.91764936085916	36.0	33.0	40.0	23.0	41.0
40	33.723266388914865	36.0	33.0	40.0	22.0	41.0
41	33.69256650285544	36.0	33.0	40.0	22.0	41.0
42	33.60053437784635	36.0	33.0	40.0	22.0	41.0
43	33.53202803838655	36.0	33.0	39.0	21.0	41.0
44	33.43954222901017	35.0	32.0	39.0	21.0	41.0
45	33.22258932560338	35.0	32.0	39.0	20.0	40.0
46	33.13549924328218	35.0	32.0	39.0	20.0	40.0
47	33.00258011338646	35.0	32.0	39.0	20.0	40.0
48	32.865195136226525	35.0	32.0	39.0	20.0	40.0
49	32.6961336394837	35.0	31.0	39.0	19.0	40.0
50	32.50548403966102	35.0	31.0	39.0	18.0	40.0
51	30.529841002677085	34.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	11.0
11	13.0
12	15.0
13	27.0
14	54.0
15	135.0
16	242.0
17	492.0
18	943.0
19	1581.0
20	2451.0
21	3636.0
22	4694.0
23	6042.0
24	6950.0
25	7814.0
26	8809.0
27	10247.0
28	11843.0
29	14473.0
30	18140.0
31	22793.0
32	28926.0
33	38885.0
34	58930.0
35	59669.0
36	59068.0
37	75953.0
38	87968.0
39	46682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.036582198256607	32.227867302517424	20.849913592175852	25.88563690705012
2	22.901709801313952	32.96120825497754	19.990857047865433	24.146224895843073
3	19.65838606115388	33.7045926018279	20.467052471540832	26.16996886547739
4	19.84141826581748	35.044000457147604	18.54287663594773	26.57170464108718
5	17.72693742272647	37.42913346285849	16.634285377856738	28.209643736558306
6	19.303750341994895	40.69618039321621	19.321759187108437	20.67831007768046
7	80.90231240497737	12.706106037465325	3.3086058036966617	3.082975753860646
8	83.62545758051166	10.09915254530783	2.9437535281751845	3.3316363460053267
9	78.01864608117141	13.792004765417476	4.866544067990317	3.3228050854208013
10	42.231780763090185	33.663899538350186	10.32738002472753	13.776939673832109
11	34.04191212376232	31.44690680768978	18.62876497418155	15.882416094366347
12	31.77297080142824	27.86903413715121	23.949339733399828	16.408655328020725
13	23.39626039404738	35.9253948958777	22.08022940498083	18.59811530509408
14	18.184258191427098	36.83328311636138	25.5084901314992	19.47396856071232
15	17.439142224854283	31.150280349233068	31.932626139838682	19.477951286073967
16	19.502367124160596	28.96549574541034	30.863350961222107	20.668786169206953
17	19.985142702781328	28.57674711771897	24.671425157663975	26.766685021835723
18	23.088378407394707	29.924466747706468	24.053063754774943	22.93409109012388
19	24.648740939299802	32.01903396398924	22.941710216902685	20.390514879808276
20	26.86971639532186	30.555295812597187	22.491142765119637	20.08384502696132
21	23.10967732998092	31.095214842058965	23.915226824867446	21.87988100309267
22	22.583438096326542	30.421095284106848	23.260501407806835	23.734965211759775
23	21.918669284875687	31.281710286167474	22.922142914038936	23.877477514917906
24	21.15554447318933	30.5705340661548	26.456725091516102	21.817196369139765
25	21.041430733479483	30.80603434840882	24.426574128908698	23.725960789203004
26	21.727498467516547	33.76294818647467	21.99711165830294	22.51244168770585
27	20.29267836548951	33.58164760153352	23.847866817663906	22.277807215313057
28	19.933540435052137	34.217325201647114	25.571001603479864	20.27813275982088
29	20.864632359816724	32.14405690795056	24.505362826280447	22.48594790595227
30	22.402310673357643	31.75063290700856	24.58207357998525	21.26498283964855
31	25.078528954413382	32.51306507080593	21.150176452049717	21.25822952273097
32	25.716457660166164	31.35911368776126	22.706209934648673	20.218218717423905
33	26.190575140174616	31.829421604380304	20.423242492562693	21.556760762882384
34	22.234863046196153	32.141459478366876	22.87902558294978	22.744651892487195
35	22.923874533761392	31.00482429254676	24.488566114972624	21.582735058719223
36	25.693946603774236	30.697115467866332	22.63469404011124	20.97424388824819
37	23.165954970960737	31.63738497715994	23.338424295317353	21.85823575656197
38	23.76821231043093	30.420575798190114	23.001624259299664	22.809587632079296
39	23.768731796347666	29.984207628131198	23.472451661835446	22.774608913685686
40	25.723903624972728	28.412935891974634	23.213228189383788	22.64993229366885
41	21.578752333357578	27.045822121095632	25.63870793462789	25.7367176109189
42	24.73843884092302	28.322372180490184	23.69150155672613	23.24768742186066
43	24.45410688249575	28.156656173051147	24.407353149989437	22.981883794463663
44	23.455828112499873	29.559960796129488	23.715397908896023	23.268813182474624
45	22.729586800901828	31.721368533699053	23.596089310018804	21.952955355380315
46	23.550374549345968	29.768101486768693	24.41393330493477	22.26759065895057
47	23.127859337066706	29.96325502948948	25.228660384350317	21.680225249093496
48	24.817920186183752	26.351788936335268	25.80026805473304	23.03002282274794
49	23.311237865674794	28.061243926343828	25.249093497075293	23.37842471090609
50	21.528881685350843	29.90403363498149	24.72094948172622	23.84613519794145
51	22.549844673710894	28.958396104548275	24.6920314323612	23.79972778937963
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11017.0
1	8799.0
2	6581.0
3	4566.5
4	2552.0
5	2328.5
6	2105.0
7	2216.0
8	2327.0
9	2424.5
10	2522.0
11	2628.5
12	2735.0
13	2722.0
14	2709.0
15	2749.5
16	2790.0
17	2611.5
18	2433.0
19	2379.5
20	2326.0
21	2243.0
22	2160.0
23	2304.5
24	2449.0
25	2979.5
26	4073.0
27	4636.0
28	6323.5
29	8011.0
30	8503.5
31	8996.0
32	9901.0
33	10806.0
34	11769.5
35	12733.0
36	14636.0
37	16539.0
38	16999.5
39	17460.0
40	20898.5
41	24337.0
42	27413.5
43	30490.0
44	32227.0
45	33964.0
46	36901.5
47	39839.0
48	40894.0
49	41949.0
50	41760.0
51	41571.0
52	39656.5
53	37742.0
54	35317.5
55	32893.0
56	31031.0
57	29169.0
58	26457.0
59	23745.0
60	23836.0
61	23927.0
62	24095.0
63	24263.0
64	21374.0
65	18485.0
66	15670.5
67	12856.0
68	11854.5
69	10853.0
70	9002.0
71	7151.0
72	6215.0
73	5279.0
74	4815.5
75	3717.0
76	3082.0
77	2414.5
78	1747.0
79	1458.5
80	1170.0
81	885.5
82	601.0
83	432.5
84	264.0
85	211.0
86	158.0
87	132.0
88	106.0
89	78.0
90	50.0
91	45.0
92	40.0
93	25.0
94	10.0
95	6.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	577494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.03224193356161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43141447077403	22.01273696966094
2	7.769398123895666	4.200484995266147
3	2.7544796226646113	2.233792786828058
4	1.310661819986154	1.4172050964379155
5	0.7393188961659777	0.9992723633606213
6	0.4813195184014702	0.7806687401264342
7	0.34626063289738856	0.6552140840385259
8	0.2633469965046037	0.5695087777591401
9	0.22590829758934844	0.549612697971087
>10	2.873126155535459	19.33947544015769
>50	1.2673289595114459	24.567907278446086
>100	0.5315449974810403	19.176933069754824
>500	6.546120658633501E-4	0.09219424135001304
>1k	0.0045822844610434515	1.7711557805609992
>5k	6.546120658633501E-4	1.6338376782815172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9233	1.5988044897436164	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2252	0.38996076149708914	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1890	0.3272761275441823	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1326	0.2296127751976644	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1263	0.21870357094619167	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	1236	0.2140281976955605	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC	1023	0.17714469760724785	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT	1019	0.17645204971826547	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31359633173678	0.0
2	0.0	0.0	0.0	0.38736333191340516	0.0
3	0.0	0.0	0.0	0.5380142477670764	0.0
4	0.0	0.0	0.0	1.2195797705257543	0.0
5	0.0	0.0	0.0	1.3529144891548657	0.0
6	0.0	0.0	0.0	1.7979407578260553	0.0
7	0.0	0.0	0.0	2.160022441791603	0.0
8	0.0	0.0	0.0	2.4803720904459614	0.0
9	0.0	0.0	0.0	3.0377804791045446	0.0
10	0.0	0.0	0.0	3.6388256847690195	0.0
11	0.0	0.0	0.0	4.36316221467236	0.0
12	0.0	0.0	0.0	4.710178807052541	0.0
13	0.0	0.0	0.0	4.908276103301506	0.0
14	0.0	0.0	0.0	5.0639487163503	0.0
15	0.0	0.0	0.0	5.182045181421798	0.0
16	0.0	0.0	0.0	5.369406435391537	0.0
17	0.0	0.0	0.0	5.595555971144289	0.0
18	0.0	0.0	0.0	5.973395394584186	0.0
19	0.0	0.0	0.0	6.09287715543365	0.0
20	0.0	0.0	0.0	6.247510796648969	0.0
21	0.0	0.0	0.0	6.379633381472361	0.0
22	0.0	0.0	0.0	6.5198945789912965	0.0
23	0.0	0.0	0.0	6.669506523011495	0.0
24	0.0	0.0	0.0	6.767862523246995	0.0
25	0.0	0.0	0.0	6.860677340370636	0.0
26	0.0	0.0	0.0	6.9481241363546635	0.0
27	0.0	0.0	0.0	7.053233453507742	0.0
28	0.0	0.0	0.0	7.1469140804926115	0.0
29	0.0	0.0	0.0	7.23903624972727	0.0
30	0.0	0.0	0.0	7.382414362746626	0.0
31	0.0	0.0	0.0	7.486657870038477	0.0
32	0.0	0.0	0.0	7.591247701274819	0.0
33	0.0	0.0	0.0	7.699993419845055	0.0
34	0.0	0.0	0.0	7.812548701804694	0.0
35	0.0	0.0	0.0	7.928394061237	0.0
36	0.0	0.0	0.0	8.0267500614725	0.0
37	0.0	0.0	0.0	8.130127758903123	0.0
38	0.0	0.0	0.0	8.241297745084797	0.0
39	0.0	0.0	0.0	8.360260020017524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAG	50	2.1827873E-11	45.000004	1
CTACTCG	25	3.8883856E-5	45.000004	14
GATCGAA	25	3.8883856E-5	45.000004	29
ACGCATT	25	3.8883856E-5	45.000004	16
CGGTTAG	25	3.8883856E-5	45.000004	1
GCACGGC	50	2.1827873E-11	45.000004	15
ATGTCGC	25	3.8883856E-5	45.000004	25
TGTGCGA	25	3.8883856E-5	45.000004	30
CGTTCAT	45	3.8380676E-10	45.000004	17
TACGCAC	25	3.8883856E-5	45.000004	33
TGCGATC	25	3.8883856E-5	45.000004	22
CTACGAC	25	3.8883856E-5	45.000004	23
CACTACC	50	2.1827873E-11	45.000004	18
TACGACA	25	3.8883856E-5	45.000004	24
CATACCG	45	3.8380676E-10	45.000004	1
AGTTATC	25	3.8883856E-5	45.000004	16
TATAGCG	25	3.8883856E-5	45.000004	1
TATGTCG	25	3.8883856E-5	45.000004	24
CCCGCTA	25	3.8883856E-5	45.000004	22
ATCCTAC	20	7.030369E-4	45.0	30
>>END_MODULE