FastQCFastQC Report
Sat 18 Jun 2016
SRR3548339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620535
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122461.9734583867146895No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30680.49441207989879704No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24070.38789109397536No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT21630.34857018540452994No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15980.25751972088600966No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT13550.21835996357981421No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTAT9640.15534981910770546No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGT9120.1469699533467089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT304.8638885E-640.00000434
TTAGCGA304.8638885E-640.00000411
TAGCGTC304.8638885E-640.00000425
TAGCGAA304.8638885E-640.00000412
TGCGGTA304.8638885E-640.0000047
ACGGGTC600.040.0000045
ATCGTAC304.8638885E-640.00000433
TCTAACG304.8638885E-640.0000041
CCGAGTG304.8638885E-640.0000041
GTACTCA304.8638885E-640.00000436
CGTACTC304.8638885E-640.00000435
ACGCTAG304.8638885E-640.0000041
TAATGAC304.8638885E-640.00000440
TCACGGC200.001255322140.06
CTTCGTT257.790502E-540.016
ACAACGC353.0533374E-740.039
GTGTTAG200.001255322140.016
TATCACG353.0533374E-740.01
CGGAATC200.001255322140.015
CGGAATA257.790502E-540.013