FastQCFastQC Report
Sat 18 Jun 2016
SRR3548338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782802
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125231.5997659689167887No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGT47870.6115211764916288No Hit
AATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT43640.5574845235449066No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC37750.48224199733776874No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28110.3590946369580047No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT25130.3210262620688245Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21540.2751653674875639No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14720.1880424424056147No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT11860.15150702220995857No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT8680.11088372283157172No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG207.0319057E-445.00000425
TCCGCGT207.0319057E-445.00000418
TCCGCGA207.0319057E-445.00000421
CTGTACG207.0319057E-445.0000041
CCGCTAG207.0319057E-445.0000041
TCCGTTA406.8102963E-945.00000410
TCCGTAA207.0319057E-445.00000422
TTACGCA207.0319057E-445.00000442
CGCAAAC207.0319057E-445.00000416
TTACCGC207.0319057E-445.00000410
CGATATA207.0319057E-445.00000437
GTAACGT207.0319057E-445.0000049
TACGTGC302.1646101E-645.00000427
GCGACGT253.889661E-545.018
CTATACG253.889661E-545.01
CACTACG700.045.01
CCGACAA253.889661E-545.024
AATCGTG453.8380676E-1045.01
AACTACG650.041.538461
ATCGTTG1250.041.39999823