##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	782802
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14016826732686	31.0	31.0	34.0	28.0	34.0
2	31.305913883715167	31.0	31.0	34.0	28.0	34.0
3	31.49529382909088	31.0	31.0	34.0	30.0	34.0
4	35.42186274434659	37.0	35.0	37.0	33.0	37.0
5	34.99378514618	35.0	35.0	37.0	32.0	37.0
6	34.972982950988886	36.0	35.0	37.0	32.0	37.0
7	34.215652233898226	36.0	35.0	37.0	31.0	37.0
8	34.55784987774686	37.0	35.0	37.0	32.0	37.0
9	35.67199879407564	39.0	35.0	39.0	31.0	39.0
10	35.28535057396379	37.0	35.0	39.0	29.0	39.0
11	35.724027276373846	37.0	35.0	39.0	30.0	39.0
12	35.74456886926707	37.0	35.0	39.0	30.0	39.0
13	35.70119774860054	37.0	35.0	39.0	30.0	39.0
14	36.77607492060572	38.0	35.0	40.0	31.0	41.0
15	36.80041440875215	38.0	35.0	40.0	31.0	41.0
16	36.70985511023222	38.0	35.0	40.0	31.0	41.0
17	36.643286552665934	38.0	35.0	40.0	31.0	41.0
18	36.47488253734661	38.0	35.0	40.0	31.0	41.0
19	36.4505494365114	38.0	35.0	40.0	30.0	41.0
20	36.40980733314427	38.0	35.0	40.0	30.0	41.0
21	36.427862984509495	38.0	35.0	40.0	30.0	41.0
22	36.449332014992294	38.0	35.0	40.0	30.0	41.0
23	36.329264616084274	38.0	35.0	40.0	30.0	41.0
24	35.94376611199256	38.0	34.0	40.0	29.0	41.0
25	36.005158392543706	38.0	34.0	40.0	30.0	41.0
26	35.84824770503908	38.0	34.0	40.0	29.0	41.0
27	35.75314958316407	38.0	34.0	40.0	29.0	41.0
28	35.63947460532804	38.0	34.0	40.0	29.0	41.0
29	35.37537844818996	38.0	34.0	40.0	27.0	41.0
30	35.458999849259456	38.0	34.0	40.0	27.0	41.0
31	35.27640961571381	38.0	34.0	40.0	27.0	41.0
32	35.149386434883915	38.0	34.0	40.0	27.0	41.0
33	35.1060012621327	38.0	34.0	40.0	26.0	41.0
34	35.05738232656534	38.0	34.0	40.0	26.0	41.0
35	34.925271013615195	38.0	33.0	40.0	25.0	41.0
36	34.73055766336826	37.0	33.0	40.0	25.0	41.0
37	34.6046982506432	37.0	33.0	40.0	24.0	41.0
38	34.451531804977506	37.0	33.0	40.0	24.0	41.0
39	34.222481547057875	37.0	33.0	40.0	23.0	41.0
40	34.079551406358185	37.0	33.0	40.0	23.0	41.0
41	34.038610018880895	37.0	33.0	40.0	23.0	41.0
42	34.00286023796567	37.0	33.0	40.0	23.0	41.0
43	33.92490438195099	37.0	33.0	40.0	23.0	41.0
44	33.880061624778676	36.0	33.0	40.0	23.0	41.0
45	33.685438974351115	36.0	33.0	40.0	23.0	41.0
46	33.51515708953222	36.0	32.0	39.0	22.0	41.0
47	33.413078913952695	36.0	32.0	39.0	22.0	40.0
48	33.266205247303915	35.0	32.0	39.0	20.0	40.0
49	33.07729924042095	35.0	32.0	39.0	20.0	40.0
50	32.88507055423977	35.0	31.0	39.0	20.0	40.0
51	30.828688480611955	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	5.0
11	14.0
12	11.0
13	28.0
14	53.0
15	116.0
16	234.0
17	450.0
18	931.0
19	1624.0
20	2739.0
21	4000.0
22	5390.0
23	6834.0
24	8046.0
25	9231.0
26	11006.0
27	12720.0
28	15754.0
29	19624.0
30	24351.0
31	30531.0
32	39414.0
33	51607.0
34	73112.0
35	80741.0
36	86615.0
37	108787.0
38	125239.0
39	63586.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.9072076974765	34.5657011607022	20.182881494937416	26.344209646883886
2	20.604827274329907	35.68028696911863	19.707410047496047	24.007475709055417
3	19.596526324664474	33.04654305941988	21.900557229031094	25.45637338688455
4	20.561521304237854	34.11130784029678	18.64392272886375	26.683248126601615
5	17.14213811410804	38.95148453887445	16.825455223670865	27.080922123346646
6	19.053221632034663	38.643871630373965	20.196678087179134	22.106228650412238
7	79.57235673899658	12.774366953584687	4.152902010981065	3.5003742964376685
8	82.13839514973135	9.759045071422914	3.089159199899847	5.013400578945889
9	76.0162850886942	14.097306854095928	5.9267860838372926	3.959621973372577
10	35.433736755910175	40.88428491495934	11.180732803441993	12.501245525688487
11	26.177373077738686	31.28351741564278	25.875764241787834	16.6633452648307
12	26.143648074481156	28.742006280004397	27.575810996905986	17.53853464860846
13	23.029322868362627	34.88519446807749	23.41473322755946	18.67074943600042
14	18.01017370931602	35.09495376864137	27.689377390451224	19.205495131591384
15	17.39405366874382	33.170967882044245	28.64415267206778	20.790825777144157
16	19.828257975835527	31.535432970278563	29.469265535857087	19.167043518028827
17	18.58273228734725	31.422249815406705	25.515519888809685	24.47949800843636
18	22.077741242357583	31.115531130477436	25.27803965753792	21.528687969627057
19	21.136123821860444	33.70430836916615	24.3811334156019	20.778434393371505
20	23.963275515392144	33.48880048850156	23.08719190804316	19.46073208806314
21	21.23806530897979	33.27495330875496	24.68772435430671	20.79925702795854
22	21.721840261011085	30.841132240336638	24.07428698444817	23.362740514204102
23	20.926747759969956	33.76907570496754	22.555895360512622	22.748281174549888
24	18.90401404186499	33.815830823120024	26.910891898589938	20.36926323642505
25	19.86032227817507	32.51358581097136	25.1331754389999	22.492916471853675
26	21.923807041882878	35.515877578238175	21.32761541232649	21.23269996755246
27	19.543000656615593	32.40972813048511	24.80640059683036	23.240870616068943
28	19.414743447257415	33.73713914885246	26.381383798201846	20.466733605688283
29	18.3442300862798	33.41496317076349	25.13713557195817	23.103671170998542
30	21.436327449342237	30.452400479303833	25.299501023247256	22.811771048106674
31	23.565090533749274	32.44894622139442	21.4847432684127	22.501219976443597
32	22.478481148489657	31.843173625003512	23.054999859479153	22.623345367027678
33	21.379224887008462	30.29463389209532	24.20957023615167	24.116570984744545
34	20.231041821558964	32.430550765072134	24.16434807269271	23.174059340676187
35	20.634208905955784	31.286327832580906	26.309334927606216	21.77012833385709
36	22.234869098443795	31.147723179041442	23.750833544114602	22.866574178400157
37	21.52536656779109	32.035814931489696	22.769359301585844	23.66945919913337
38	21.630501710521948	31.141463614042884	25.454329447293183	21.77370522814198
39	22.599712315502515	30.352758424224774	24.525358903017622	22.522170357255092
40	25.143650629405652	29.53479934900524	24.47924251598744	20.842307505601674
41	21.010804775664855	27.783781850327415	27.170216734244416	24.035196639763313
42	25.304994110899052	29.015383200349515	23.204846180771128	22.474776507980305
43	22.47311580706232	28.83053441355541	24.644546130439117	24.051803648943153
44	21.794655608953477	30.54131185152823	24.36197148193285	23.302061057585444
45	23.736781459423966	31.04016085804584	22.70484745823337	22.51821022429682
46	20.889445862427536	29.998134905122882	26.71492919026778	22.397490042181804
47	22.258885388642337	30.208405190584593	25.19462137296532	22.338088047807748
48	23.191943812100632	27.706112145855528	27.144922981801272	21.957021060242564
49	22.333616929951635	28.742134026228854	25.786341884665596	23.13790715915391
50	20.86159718549518	29.90321946034885	24.508624147613318	24.72655920654265
51	20.422533412025007	29.220160398159432	26.41383133921477	23.94347485060079
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14503.0
1	11217.5
2	7932.0
3	5342.0
4	2752.0
5	2587.0
6	2422.0
7	2702.5
8	2983.0
9	3228.5
10	3474.0
11	3821.5
12	4169.0
13	4522.5
14	4876.0
15	4959.5
16	5043.0
17	4630.5
18	4218.0
19	4082.0
20	3946.0
21	4061.5
22	4177.0
23	4874.0
24	5571.0
25	5918.5
26	7374.0
27	8482.0
28	9337.0
29	10192.0
30	11939.5
31	13687.0
32	15286.0
33	16885.0
34	18842.0
35	20799.0
36	23071.0
37	25343.0
38	26493.5
39	27644.0
40	31302.0
41	34960.0
42	38228.0
43	41496.0
44	44928.5
45	48361.0
46	55425.0
47	62489.0
48	62780.5
49	63072.0
50	63565.0
51	64058.0
52	60641.5
53	57225.0
54	51268.0
55	45311.0
56	39806.0
57	34301.0
58	31269.0
59	28237.0
60	26841.0
61	25445.0
62	23269.0
63	21093.0
64	19047.0
65	17001.0
66	14022.0
67	11043.0
68	10045.0
69	9047.0
70	7849.0
71	6651.0
72	5872.0
73	5093.0
74	5092.0
75	4473.0
76	3855.0
77	2883.0
78	1911.0
79	1326.0
80	741.0
81	605.0
82	469.0
83	343.5
84	218.0
85	184.0
86	150.0
87	111.0
88	72.0
89	47.5
90	23.0
91	16.5
92	10.0
93	8.5
94	7.0
95	5.5
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	782802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.124720808465455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2966771722109	24.680869380240804
2	8.184457528050935	5.094779110586574
3	3.070672292498136	2.8672145339488515
4	1.6414419173957504	2.0435768560901977
5	0.9785857772668355	1.5229104552282708
6	0.7157821406831156	1.336711157306863
7	0.5333727080498148	1.1620753637433952
8	0.4249849363129494	1.0582029992435218
9	0.3484753747707485	0.9761578873528423
>10	3.716242251869563	26.07421457938259
>50	0.8623687548976017	18.754086444601988
>100	0.22194232076076453	9.523949346138739
>500	0.0012492063082219392	0.2932938337118047
>1k	0.0033312168219251717	2.9889272616268845
>5k	0.0	0.0
>10k+	4.1640210274064646E-4	1.623030790796699
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12523	1.5997659689167887	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGT	4787	0.6115211764916288	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	4364	0.5574845235449066	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC	3775	0.48224199733776874	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2811	0.3590946369580047	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	2513	0.3210262620688245	Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2154	0.2751653674875639	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1472	0.1880424424056147	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	1186	0.15150702220995857	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	868	0.11088372283157172	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5549244892067213E-4	0.0	0.0	0.48990677080538886	0.0
2	2.5549244892067213E-4	0.0	0.0	0.6246790376110434	0.0
3	2.5549244892067213E-4	0.0	0.0	0.8842593657144463	0.0
4	2.5549244892067213E-4	0.0	0.0	2.2319820337709917	0.0
5	2.5549244892067213E-4	0.0	0.0	2.4174695516874	0.0
6	2.5549244892067213E-4	0.0	0.0	3.175771140083955	0.0
7	2.5549244892067213E-4	0.0	0.0	3.777455857292138	0.0
8	2.5549244892067213E-4	0.0	0.0	4.14715343088035	0.0
9	2.5549244892067213E-4	0.0	0.0	5.497558769650563	0.0
10	2.5549244892067213E-4	0.0	0.0	6.295461687629822	0.0
11	2.5549244892067213E-4	0.0	0.0	7.661963050682037	0.0
12	2.5549244892067213E-4	0.0	0.0	8.05427170599973	0.0
13	2.5549244892067213E-4	0.0	0.0	8.277316613907477	0.0
14	2.5549244892067213E-4	0.0	0.0	8.53357554017491	0.0
15	2.5549244892067213E-4	0.0	0.0	8.694152544321552	0.0
16	2.5549244892067213E-4	0.0	0.0	8.983242250275293	0.0
17	2.5549244892067213E-4	0.0	0.0	9.35447277855703	0.0
18	2.5549244892067213E-4	0.0	0.0	9.811037784778271	0.0
19	2.5549244892067213E-4	0.0	0.0	10.01211034207884	0.0
20	2.5549244892067213E-4	0.0	0.0	10.225702029376523	0.0
21	2.5549244892067213E-4	0.0	0.0	10.465098454015191	0.0
22	2.5549244892067213E-4	0.0	0.0	10.712031905897021	0.0
23	2.5549244892067213E-4	0.0	0.0	10.980171231039266	0.0
24	2.5549244892067213E-4	0.0	0.0	11.180221818544153	0.0
25	2.5549244892067213E-4	0.0	0.0	11.345397686771367	0.0
26	2.5549244892067213E-4	0.0	0.0	11.515044672854694	0.0
27	2.5549244892067213E-4	0.0	0.0	11.682903211795576	0.0
28	2.5549244892067213E-4	0.0	0.0	11.851400481858759	0.0
29	2.5549244892067213E-4	0.0	0.0	12.029861957429848	0.0
30	2.5549244892067213E-4	0.0	0.0	12.275773439515994	0.0
31	2.5549244892067213E-4	0.0	0.0	12.46381588192161	0.0
32	2.5549244892067213E-4	0.0	0.0	12.647131714022192	0.0
33	2.5549244892067213E-4	0.0	0.0	12.830447546122775	0.0
34	2.5549244892067213E-4	0.0	0.0	13.007759305673721	0.0
35	2.5549244892067213E-4	0.0	0.0	13.205638207362782	0.0
36	2.5549244892067213E-4	0.0	0.0	13.388187562116602	0.0
37	2.5549244892067213E-4	0.0	0.0	13.571503394217183	0.0
38	2.5549244892067213E-4	0.0	0.0	13.768615818559482	0.0
39	2.5549244892067213E-4	0.0	0.0	13.982846236979466	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCCG	20	7.0319057E-4	45.000004	25
TCCGCGT	20	7.0319057E-4	45.000004	18
TCCGCGA	20	7.0319057E-4	45.000004	21
CTGTACG	20	7.0319057E-4	45.000004	1
CCGCTAG	20	7.0319057E-4	45.000004	1
TCCGTTA	40	6.8102963E-9	45.000004	10
TCCGTAA	20	7.0319057E-4	45.000004	22
TTACGCA	20	7.0319057E-4	45.000004	42
CGCAAAC	20	7.0319057E-4	45.000004	16
TTACCGC	20	7.0319057E-4	45.000004	10
CGATATA	20	7.0319057E-4	45.000004	37
GTAACGT	20	7.0319057E-4	45.000004	9
TACGTGC	30	2.1646101E-6	45.000004	27
GCGACGT	25	3.889661E-5	45.0	18
CTATACG	25	3.889661E-5	45.0	1
CACTACG	70	0.0	45.0	1
CCGACAA	25	3.889661E-5	45.0	24
AATCGTG	45	3.8380676E-10	45.0	1
AACTACG	65	0.0	41.53846	1
ATCGTTG	125	0.0	41.399998	23
>>END_MODULE