FastQCFastQC Report
Sat 18 Jun 2016
SRR3548336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582047
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113720.7188155598411425No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24840.15701177019393228No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23240.14689829063232634No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG20050.1267345407563745No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT16820.10631795389138249TruSeq Adapter, Index 21 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT16130.10195651583043994No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG650.045.0000041
TCGATTG253.891474E-545.01
TAACGCG253.891474E-545.01
ATATCGA207.034094E-445.011
GCGTCTA253.891474E-545.022
CCCGTAG302.1660217E-644.9999961
GTATACG302.1660217E-644.9999961
CAGTACG603.6379788E-1241.2499961
AATACGG2000.040.52
TAGTACG501.0822987E-940.51
CGTAACG950.040.2631571
TTTAGCG451.9290383E-840.0000041
CGATACG451.9290383E-840.0000041
TAGACGG2250.040.02
CCGACGT403.4593177E-739.37542
TCATACG1200.039.3749961
GTAGGGC3150.038.571434
TTAACGG2800.038.5714262
GTACGAC356.249782E-638.57142612
TCTAACG659.094947E-1238.0769271
Produced by FastQC (version 0.11.5)