FastQCFastQC Report
Sat 18 Jun 2016
SRR3548335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670301
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143580.8596055441504256No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32220.19289936364762997No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT29180.17469905124884677No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28470.170448320392552No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT22810.1365622124395543No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17670.10578931581792742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG600.040.0000041
TCGATTG304.8688416E-640.0000041
ACACGAT451.2223609E-940.038
CGTATAA257.796175E-540.029
GTTTCGA200.001255932140.018
TCTTACG353.0574847E-740.01
TACGATT200.001255932140.011
TCATACG800.040.01
CGACTCA200.001255932140.01
GATTCGT401.9292202E-840.09
TTATTCG257.796175E-540.030
GGTTAAC200.001255932140.038
TAGTCGA200.001255932140.024
TACGTCG257.796175E-540.01
TAGTACG650.040.01
TACCGAC200.001255932140.03
CAGTACG950.039.9999961
CGTAGCG1150.038.260871
TACGTAG800.037.51
GAATACG650.036.9230771
Produced by FastQC (version 0.11.5)