##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1670301
Sequences flagged as poor quality	0
Sequence length	46
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217190195060653	31.0	31.0	34.0	28.0	34.0
2	31.519108232588017	31.0	31.0	34.0	30.0	34.0
3	31.629941549457254	31.0	31.0	34.0	30.0	34.0
4	35.59291049936508	37.0	35.0	37.0	33.0	37.0
5	35.1986845484736	37.0	35.0	37.0	33.0	37.0
6	35.36096128781579	37.0	35.0	37.0	33.0	37.0
7	35.38673388808365	37.0	35.0	37.0	33.0	37.0
8	35.79200994311804	37.0	35.0	37.0	35.0	37.0
9	37.113976462924946	39.0	37.0	39.0	34.0	39.0
10	36.29696623542703	38.0	35.0	39.0	32.0	39.0
11	36.53624227010581	38.0	35.0	39.0	32.0	39.0
12	36.45198500150572	38.0	35.0	39.0	32.0	39.0
13	36.29088828899701	38.0	35.0	39.0	32.0	39.0
14	37.496972102632995	39.0	36.0	41.0	33.0	41.0
15	37.34722124934368	39.0	36.0	40.0	32.0	41.0
16	37.2430855276983	39.0	36.0	40.0	32.0	41.0
17	37.19025732487737	39.0	36.0	40.0	32.0	41.0
18	37.05395793931753	39.0	35.0	40.0	32.0	41.0
19	37.33699973837051	39.0	35.0	40.0	32.0	41.0
20	37.14679988816387	39.0	35.0	40.0	32.0	41.0
21	37.25767511364718	39.0	35.0	40.0	32.0	41.0
22	37.32868147717088	39.0	35.0	40.0	32.0	41.0
23	37.210224384706706	39.0	35.0	40.0	32.0	41.0
24	36.81061257821195	39.0	35.0	40.0	31.0	41.0
25	36.97540922264909	39.0	35.0	40.0	31.0	41.0
26	37.00997365145564	39.0	35.0	40.0	32.0	41.0
27	36.926778466875135	39.0	35.0	40.0	31.0	41.0
28	36.9031006986166	39.0	35.0	40.0	31.0	41.0
29	36.5855567349837	39.0	35.0	40.0	31.0	41.0
30	36.61258958714627	38.0	35.0	40.0	31.0	41.0
31	36.5603211636705	38.0	35.0	40.0	31.0	41.0
32	36.585026890362876	38.0	35.0	40.0	31.0	41.0
33	36.55300691312524	39.0	35.0	40.0	31.0	41.0
34	36.451636561314395	38.0	35.0	40.0	31.0	41.0
35	36.24815048305665	38.0	35.0	40.0	30.0	41.0
36	36.221439129833485	38.0	35.0	40.0	30.0	41.0
37	36.135474384557035	38.0	35.0	40.0	30.0	41.0
38	35.95852963028819	38.0	35.0	40.0	30.0	41.0
39	35.8886434241493	38.0	35.0	40.0	30.0	41.0
40	35.72545427440922	38.0	34.0	40.0	29.0	41.0
41	35.73509265695225	38.0	34.0	40.0	29.0	41.0
42	35.62677445562207	38.0	34.0	40.0	29.0	41.0
43	35.59640687516801	38.0	34.0	40.0	29.0	41.0
44	35.49768275298883	37.0	34.0	40.0	29.0	41.0
45	35.28983578408921	37.0	34.0	40.0	28.0	41.0
46	34.21257186578946	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	11.0
10	21.0
11	25.0
12	37.0
13	36.0
14	53.0
15	105.0
16	153.0
17	301.0
18	584.0
19	998.0
20	1705.0
21	2669.0
22	4075.0
23	6117.0
24	8032.0
25	10579.0
26	12810.0
27	15972.0
28	19924.0
29	25531.0
30	33117.0
31	44277.0
32	60655.0
33	86883.0
34	138585.0
35	154708.0
36	177222.0
37	251165.0
38	361810.0
39	252132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.655976976604816	29.818936826356445	21.316038246998595	27.209047950040144
2	24.612031005190083	29.379914159184484	19.840016859236748	26.168037976388685
3	20.270717673042164	31.77505132308488	20.311668375939426	27.642562627933525
4	20.186481358749113	32.88970071861299	19.26718597426452	27.656631948373377
5	19.126552639314713	34.49150781805196	16.86905533793011	29.512884204703223
6	20.461102519845227	37.194254209271264	18.955625363332718	23.389017907550794
7	87.37036019256409	6.323710516846963	3.0471753294765436	3.258753961112398
8	89.35048233821328	5.514754526279994	2.5190669226684292	2.615696212838285
9	83.44268488134773	8.544627585087957	4.389208891092085	3.6234786424722247
10	40.49120487864163	33.96339941124384	10.796437288847937	14.748958421266586
11	30.031114152479105	27.26023632866172	23.072548001827215	19.63610151703196
12	28.91939835993632	23.831932088886973	27.40625791399275	19.842411637183957
13	25.575090956659906	26.82372817833432	26.218567791074783	21.382613073930987
14	21.655617759912733	28.177855368583266	28.26269037736312	21.903836494140876
15	21.676452328053447	27.283585413647	29.020278380962473	22.01968387733708
16	24.534200721905812	25.672917635803365	27.459601592766813	22.333280049524006
17	23.529351895257204	25.82977559134551	26.030996808359692	24.609875705037595
18	24.84426459662061	26.515999212118057	25.8204359573514	22.819300233909935
19	24.984359106532295	29.355726901917677	23.33836835396734	22.321545637582688
20	26.692075260686547	28.15372798076514	23.465830410207502	21.688366348340807
21	26.146425105415133	27.82654144372781	24.244013504152846	21.783019946704215
22	24.696087711137093	26.043629262031214	24.511510200856012	24.748772825975678
23	24.76385992704309	27.763259436472826	23.579881709943297	23.892998926540784
24	23.933829890540686	27.134749964228007	26.01345505989639	22.917965085334917
25	23.072847349070617	27.57383250084865	23.521030041890654	25.83229010819008
26	24.453676313430933	28.396738072958104	22.874559735041768	24.275025878569192
27	22.554198315153975	26.91993838236342	24.785592536914006	25.740270765568603
28	22.09793324676211	29.142292317372736	25.360339244244003	23.39943519162115
29	22.836901851821917	28.597300726036806	24.92802195532422	23.63777546681706
30	24.87024793734782	26.63226568145502	25.9423301548643	22.55515622633286
31	26.281909667778443	28.21970411321073	23.09691486744006	22.401471351570766
32	27.420626581676	26.520070334628308	23.849354098452913	22.209948985242782
33	25.695009462366365	26.43655245371942	23.973104248874904	23.895333835039313
34	23.403386575234045	28.584488664019243	25.530907303533912	22.4812174572128
35	24.030519050159224	28.81833873056413	25.637415052736003	21.51372716654064
36	25.692435076073117	29.83192849672005	23.674475438858025	20.80116098834881
37	24.66842802584684	29.650224719975622	23.32130556109348	22.36004169308406
38	25.127566827775354	28.747992128364885	23.656035648664524	22.468405395195237
39	25.134332075476216	27.682435680754548	23.494986831714762	23.688245412054474
40	25.578383776337322	26.791578284393054	24.019083985461304	23.610953953808327
41	22.25383329112537	26.92053707685022	26.15127453075823	24.67435510126618
42	23.994896728194497	27.06631918438653	23.817264073960324	25.121520013458653
43	23.78565300505717	26.386441725174087	24.912994723705488	24.914910546063254
44	23.80852313445301	26.433439242388047	24.81905955872624	24.9389780644327
45	24.086018029085775	27.658128684590384	24.70638525631009	23.549468030013752
46	24.148521733507913	26.401768304036217	26.166241892928284	23.283468069527586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17208.0
1	13622.5
2	10037.0
3	6902.0
4	3767.0
5	3694.0
6	3621.0
7	3621.0
8	3738.5
9	3856.0
10	3932.5
11	4009.0
12	4276.5
13	4544.0
14	4327.5
15	4111.0
16	4625.5
17	5140.0
18	4916.5
19	4693.0
20	4693.0
21	5345.0
22	5997.0
23	7298.0
24	8599.0
25	10208.0
26	11817.0
27	14789.0
28	17761.0
29	20539.0
30	23317.0
31	23317.0
32	27374.0
33	31431.0
34	33813.5
35	36196.0
36	41630.0
37	47064.0
38	52783.0
39	58502.0
40	66827.0
41	75152.0
42	86329.0
43	97506.0
44	97506.0
45	102791.0
46	108076.0
47	111635.0
48	115194.0
49	115957.0
50	116720.0
51	112366.0
52	108012.0
53	103452.0
54	98892.0
55	98366.5
56	97841.0
57	97841.0
58	98588.0
59	99335.0
60	97222.5
61	95110.0
62	93042.5
63	90975.0
64	83970.0
65	76965.0
66	69204.0
67	61443.0
68	53618.0
69	45793.0
70	45793.0
71	38370.0
72	30947.0
73	26070.0
74	21193.0
75	17281.0
76	13369.0
77	10352.0
78	7335.0
79	6478.0
80	5621.0
81	5621.0
82	3786.5
83	1952.0
84	1359.5
85	767.0
86	486.0
87	205.0
88	162.5
89	120.0
90	81.5
91	43.0
92	33.5
93	24.0
94	24.0
95	21.5
96	19.0
97	20.0
98	21.0
99	11.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1670301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.232730923893467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95621044688453	14.416126265996626
2	8.990678075496959	3.4583058449876267
3	3.419995601183183	1.9732756547536632
4	1.8638789770733077	1.433899313630109
5	1.1705268844429846	1.125621430383763
6	0.8192171018617553	0.9453469253015379
7	0.6172355022161174	0.8309787031557785
8	0.48824956732647934	0.7512298041678067
9	0.37993857768144723	0.6576530788938664
>10	4.501250926939536	20.577727440725237
>50	1.8164272204127994	25.298471042027714
>100	0.9710744467915916	26.28514231461083
>500	0.0025019631479103847	0.36336300871970373
>1k	0.0025019631479103847	1.019232938711369
>5k	0.0	0.0
>10k+	3.127453934887981E-4	0.8636262339343661
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14358	0.8596055441504256	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3222	0.19289936364762997	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	2918	0.17469905124884677	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2847	0.170448320392552	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT	2281	0.1365622124395543	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1767	0.10578931581792742	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1961921833250414	0.0
2	0.0	0.0	0.0	0.27210664425154507	0.0
3	0.0	0.0	0.0	0.36849645662667985	0.0
4	0.0	0.0	0.0	0.7572886563559502	0.0
5	0.0	0.0	0.0	0.8449973986724548	0.0
6	0.0	0.0	0.0	1.264861842266753	0.0
7	0.0	0.0	0.0	1.5799547506706875	0.0
8	0.0	0.0	0.0	1.8778651273034022	0.0
9	0.0	0.0	0.0	2.308625810557498	0.0
10	0.0	0.0	0.0	2.9359378938287173	0.0
11	0.0	0.0	0.0	3.50966682053115	0.0
12	0.0	0.0	0.0	3.7750680865305117	0.0
13	0.0	0.0	0.0	3.9075591764598117	0.0
14	0.0	0.0	0.0	4.031488935227842	0.0
15	0.0	0.0	0.0	4.119856241479829	0.0
16	0.0	0.0	0.0	4.282282055749233	0.0
17	0.0	0.0	0.0	4.475121549948183	0.0
18	0.0	0.0	0.0	4.720885636780436	0.0
19	0.0	0.0	0.0	4.844755526099787	0.0
20	0.0	0.0	0.0	4.970541237776904	0.0
21	0.0	0.0	0.0	5.118478645465697	0.0
22	0.0	0.0	0.0	5.280844590286422	0.0
23	0.0	0.0	0.0	5.4388400653534905	0.0
24	0.0	0.0	0.0	5.558159876573145	0.0
25	0.0	0.0	0.0	5.670534831745895	0.0
26	0.0	0.0	0.0	5.775964930871741	0.0
27	0.0	0.0	0.0	5.8912136195811415	0.0
28	0.0	0.0	0.0	6.005205049868257	0.0
29	0.0	0.0	0.0	6.130451936507252	0.0
30	0.0	0.0	0.0	6.296110701005388	0.0
31	0.0	0.0	0.0	6.417945029069611	0.0
32	0.0	0.0	0.0	6.556722411110333	0.0
33	0.0	0.0	0.0	6.697774832200903	0.0
34	0.0	0.0	0.0	6.833139655666852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	60	0.0	40.000004	1
TCGATTG	30	4.8688416E-6	40.000004	1
ACACGAT	45	1.2223609E-9	40.0	38
CGTATAA	25	7.796175E-5	40.0	29
GTTTCGA	20	0.0012559321	40.0	18
TCTTACG	35	3.0574847E-7	40.0	1
TACGATT	20	0.0012559321	40.0	11
TCATACG	80	0.0	40.0	1
CGACTCA	20	0.0012559321	40.0	1
GATTCGT	40	1.9292202E-8	40.0	9
TTATTCG	25	7.796175E-5	40.0	30
GGTTAAC	20	0.0012559321	40.0	38
TAGTCGA	20	0.0012559321	40.0	24
TACGTCG	25	7.796175E-5	40.0	1
TAGTACG	65	0.0	40.0	1
TACCGAC	20	0.0012559321	40.0	3
CAGTACG	95	0.0	39.999996	1
CGTAGCG	115	0.0	38.26087	1
TACGTAG	80	0.0	37.5	1
GAATACG	65	0.0	36.923077	1
>>END_MODULE