##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548334_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744107
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112348089723657	31.0	31.0	34.0	28.0	34.0
2	31.275026306700514	31.0	31.0	34.0	28.0	34.0
3	31.46629181018321	31.0	31.0	34.0	30.0	34.0
4	35.397764031248194	37.0	35.0	37.0	33.0	37.0
5	34.981336017535114	35.0	35.0	37.0	32.0	37.0
6	34.96380896833386	36.0	35.0	37.0	32.0	37.0
7	34.12375236357137	35.0	35.0	37.0	30.0	37.0
8	34.459372106430926	37.0	35.0	37.0	32.0	37.0
9	35.558333680505626	39.0	35.0	39.0	30.0	39.0
10	35.27216381515024	37.0	35.0	39.0	29.0	39.0
11	35.70580306326913	37.0	35.0	39.0	30.0	39.0
12	35.650250568802605	37.0	35.0	39.0	30.0	39.0
13	35.55825976640456	37.0	35.0	39.0	30.0	39.0
14	36.58801623959995	38.0	35.0	40.0	31.0	41.0
15	36.68049084338677	38.0	35.0	40.0	31.0	41.0
16	36.64064173566436	38.0	35.0	40.0	31.0	41.0
17	36.566820363200456	38.0	35.0	40.0	31.0	41.0
18	36.34206639636504	38.0	35.0	40.0	30.0	41.0
19	36.30469005129638	38.0	35.0	40.0	30.0	41.0
20	36.188063007067534	38.0	34.0	40.0	30.0	41.0
21	36.191335385905525	38.0	34.0	40.0	30.0	41.0
22	36.10311017098348	38.0	34.0	40.0	30.0	41.0
23	36.02460264451215	38.0	34.0	40.0	30.0	41.0
24	35.66602652575503	38.0	34.0	40.0	29.0	41.0
25	35.66669578434284	38.0	34.0	40.0	29.0	41.0
26	35.50167516230864	38.0	34.0	40.0	28.0	41.0
27	35.345500042332624	38.0	34.0	40.0	27.0	41.0
28	35.17299393769982	37.0	33.0	40.0	27.0	41.0
29	34.78444632290786	37.0	33.0	40.0	25.0	41.0
30	34.85485823947362	37.0	33.0	40.0	25.0	41.0
31	34.616961001576385	37.0	33.0	40.0	25.0	41.0
32	34.523967655189374	37.0	33.0	40.0	24.0	41.0
33	34.36712865219653	37.0	33.0	40.0	24.0	41.0
34	34.28769115194454	37.0	33.0	40.0	24.0	41.0
35	34.08172346181396	37.0	33.0	40.0	23.0	41.0
36	33.94509929351558	36.0	33.0	40.0	23.0	41.0
37	33.82159420620959	36.0	33.0	40.0	22.0	41.0
38	33.61630786970154	36.0	32.0	40.0	22.0	41.0
39	33.4489851593924	36.0	32.0	39.0	21.0	41.0
40	33.235623371369975	35.0	31.0	39.0	21.0	41.0
41	33.20785182776133	35.0	32.0	39.0	20.0	41.0
42	33.07355796948557	35.0	31.0	39.0	20.0	40.0
43	33.05037985128483	35.0	32.0	39.0	20.0	40.0
44	32.996301607161335	35.0	31.0	39.0	20.0	40.0
45	32.83778005044974	35.0	31.0	39.0	20.0	40.0
46	32.65888239191407	35.0	31.0	39.0	19.0	40.0
47	32.50844569396605	35.0	31.0	39.0	18.0	40.0
48	32.39476043095953	35.0	31.0	39.0	18.0	40.0
49	32.10966433590868	35.0	31.0	39.0	17.0	40.0
50	31.978806811385997	35.0	30.0	38.0	16.0	40.0
51	29.811287892735855	34.0	26.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	11.0
11	24.0
12	29.0
13	37.0
14	72.0
15	153.0
16	346.0
17	663.0
18	1210.0
19	2159.0
20	3485.0
21	5089.0
22	6809.0
23	8497.0
24	9686.0
25	11143.0
26	12521.0
27	14568.0
28	17294.0
29	20547.0
30	25475.0
31	31322.0
32	39611.0
33	50848.0
34	71763.0
35	76731.0
36	79713.0
37	97727.0
38	104608.0
39	51954.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.709127853924237	34.51345035055443	19.697167208479428	26.080254587041917
2	22.34786126188841	34.78048183930537	20.06929111001509	22.80236578879113
3	20.69003516967318	32.72795444741146	20.688960055475892	25.893050327439465
4	20.757632974827544	34.217659557026074	18.282854481949503	26.74185298619688
5	18.101428961157467	36.74982227018426	17.407442746809263	27.74130602184901
6	19.765436960007097	38.952865649698225	18.9224130400601	22.359284350234574
7	79.1517886540511	12.646837081226222	4.304354078109735	3.897020186612947
8	81.66285225108754	10.03363763544759	3.665601855647105	4.6379082578177595
9	76.66585585137621	13.393235112692125	5.766509386418889	4.174399649512772
10	40.3759136790811	32.28137888771373	11.562046856164503	15.780660577040667
11	30.363912716853893	29.784426164516663	21.039312894516517	18.81234822411293
12	27.30077797951101	25.719688163127074	27.678008673483788	19.301525183878123
13	25.04451644723138	30.728779597557875	23.971014921241167	20.255689033969578
14	18.61802133295346	33.23675224127713	28.170814143664824	19.974412282104588
15	17.95185369846003	29.249153683542822	32.11742397262759	20.68156864536955
16	19.949684655567008	27.96076370737004	28.914927557461496	23.174624079601454
17	20.340891834104504	27.351442736058118	24.4638203914222	27.84384503841517
18	23.65009333335125	28.37831118374105	24.20243325220701	23.76916223070069
19	23.919678218320755	30.297390025896814	23.1963951420965	22.586536613685933
20	25.033227748159874	29.79074246042572	22.552804905746083	22.623224885668325
21	24.85207100591716	29.075657129955772	23.980019002643438	22.09225286148363
22	22.94616231267815	30.309485060616282	22.70130505424623	24.043047572459336
23	22.667976514130363	29.604882093569877	22.026267727625193	25.700873664674567
24	20.873342140310466	30.395225417849854	24.456832149139842	24.27460029269984
25	21.837450796726817	28.64991190783046	23.586124038612727	25.92651325683
26	23.396635161341045	31.451659505958148	20.84108871439188	24.310616618308924
27	20.118880752364916	29.854980533713565	24.370419845532968	25.65571886838855
28	22.83408165761107	28.98911043707424	24.484247561170637	23.692560344144052
29	21.741631243893686	30.588477194812036	24.11508022367751	23.55481133761677
30	23.586392817162046	28.732964479570818	23.50952215205609	24.171120551211047
31	26.60598542951484	30.61548943901885	19.674455421061758	23.104069710404552
32	26.385183851247202	29.748813006731563	20.810851127593207	23.05515201442803
33	25.468380219511445	27.847339159556356	22.947774983974078	23.736505636958125
34	25.567156336387104	28.984809980285092	22.03406230555552	23.413971377772285
35	24.2990591406881	28.853242880392198	24.21667851532105	22.631019463598648
36	27.27900691701597	26.931745031292543	24.423234830474648	21.366013221216843
37	24.899241641323087	29.146077109878014	23.17193629410824	22.782744954690656
38	25.001915047163912	28.17901188941913	23.768490284327388	23.050582779089567
39	25.456419574066636	27.459088545061395	23.932176420864202	23.15231546000777
40	26.383705569225928	26.666594992386848	23.839447821348273	23.110251617038948
41	24.157816013019634	25.714312592140647	25.962260803889762	24.165610590949957
42	25.22889853206595	25.967367596326874	24.359668703560107	24.444065168047068
43	24.624146796092496	26.54134418840301	24.3127668467035	24.52174216880099
44	22.914177665308888	27.198507741494165	24.81377006263884	25.073544530558106
45	24.594446766392466	27.7430530824196	24.358862367912142	23.303637783275793
46	23.926532071328452	26.8688508507513	27.000149172094872	22.20446790582537
47	23.58491453514078	26.512316105076284	25.418118630788317	24.48465072899462
48	24.491907749826304	25.00823134307297	27.01130348189172	23.48855742520901
49	23.680868477248566	25.28587958452212	26.984560016234227	24.04869192199509
50	22.79215220391691	26.400773007107848	25.96373908591103	24.84333570306421
51	23.109579670665646	26.329412302262984	26.247703623269235	24.313304403802142
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14367.0
1	11121.5
2	7876.0
3	5290.0
4	2704.0
5	2478.5
6	2253.0
7	2403.0
8	2553.0
9	2858.0
10	3163.0
11	3291.0
12	3419.0
13	3555.0
14	3691.0
15	3641.0
16	3591.0
17	3391.5
18	3192.0
19	3203.5
20	3215.0
21	3120.5
22	3026.0
23	3116.0
24	3206.0
25	3360.0
26	4683.5
27	5853.0
28	6992.5
29	8132.0
30	8472.0
31	8812.0
32	10734.5
33	12657.0
34	14377.5
35	16098.0
36	16922.0
37	17746.0
38	18912.0
39	20078.0
40	22734.0
41	25390.0
42	28887.0
43	32384.0
44	35759.0
45	39134.0
46	45106.5
47	51079.0
48	50879.5
49	50680.0
50	50248.0
51	49816.0
52	47435.5
53	45055.0
54	42491.0
55	39927.0
56	38529.5
57	37132.0
58	36983.0
59	36834.0
60	37076.0
61	37318.0
62	35144.0
63	32970.0
64	30438.0
65	27906.0
66	26012.0
67	24118.0
68	21326.5
69	18535.0
70	17047.5
71	15560.0
72	13315.0
73	11070.0
74	8921.0
75	6179.0
76	5586.0
77	4176.0
78	2766.0
79	2413.5
80	2061.0
81	1611.5
82	1162.0
83	1024.5
84	887.0
85	652.5
86	418.0
87	306.0
88	194.0
89	152.5
90	111.0
91	85.5
92	60.0
93	39.0
94	18.0
95	13.5
96	9.0
97	8.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	744107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.630134233529166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3862391058725	24.114851894691032
2	7.70493324252102	4.565964124725579
3	2.852026045044682	2.535177436565857
4	1.3654648204184272	1.6183562368063917
5	0.8170310344215029	1.2104369611434163
6	0.5146715400192607	0.9149872090168725
7	0.3799590276451499	0.7880765894656909
8	0.31185660891015954	0.7392282546896988
9	0.2382670105126134	0.6353895154419396
>10	2.916633250571184	21.24094901309597
>50	1.1975728708513123	24.89281433142884
>100	0.3093344596905569	12.053134868265536
>500	0.0023119167390923535	0.5251394238208352
>1k	0.003236683434729295	2.492939461477672
>5k	0.0	0.0
>10k+	4.6238334781847066E-4	1.672554679364664
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12208	1.640624265058654	No Hit
AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	3743	0.5030190550552541	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT	3330	0.44751628462035703	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2621	0.3522342888858726	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	2614	0.3512935639632472	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2275	0.30573559985324694	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	2107	0.2831582017102379	Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1506	0.20239024763911642	No Hit
AATGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	969	0.13022320714628405	No Hit
AATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	842	0.11315576926436656	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.031678239823036E-4	0.0	0.0	0.4062587772995013	0.0
2	4.031678239823036E-4	0.0	0.0	0.507722679668381	0.0
3	4.031678239823036E-4	0.0	0.0	0.7361844465916864	0.0
4	4.031678239823036E-4	0.0	0.0	1.8620977896995996	0.0
5	4.031678239823036E-4	0.0	0.0	2.139880420423407	0.0
6	4.031678239823036E-4	0.0	0.0	2.8106172902552995	0.0
7	4.031678239823036E-4	0.0	0.0	3.3690047264707896	0.0
8	4.031678239823036E-4	0.0	0.0	3.751745380704657	0.0
9	4.031678239823036E-4	0.0	0.0	4.72539567562192	0.0
10	4.031678239823036E-4	0.0	0.0	5.454591879931247	0.0
11	4.031678239823036E-4	0.0	0.0	6.467483843049454	0.0
12	4.031678239823036E-4	0.0	0.0	6.842295530078336	0.0
13	4.031678239823036E-4	0.0	0.0	7.092259580947363	0.0
14	4.031678239823036E-4	0.0	0.0	7.312389212841701	0.0
15	4.031678239823036E-4	0.0	0.0	7.428501546148605	0.0
16	4.031678239823036E-4	0.0	0.0	7.653200413381409	0.0
17	4.031678239823036E-4	0.0	0.0	7.878302448438195	0.0
18	4.031678239823036E-4	0.0	0.0	8.301091106520971	0.0
19	5.375570986430715E-4	0.0	0.0	8.422310232264984	0.0
20	5.375570986430715E-4	0.0	0.0	8.607498652747521	0.0
21	5.375570986430715E-4	0.0	0.0	8.752101512282508	0.0
22	5.375570986430715E-4	0.0	0.0	8.909471352910266	0.0
23	5.375570986430715E-4	0.0	0.0	9.075710885665638	0.0
24	5.375570986430715E-4	0.0	0.0	9.195451729388381	0.0
25	5.375570986430715E-4	0.0	0.0	9.297184410306583	0.0
26	5.375570986430715E-4	0.0	0.0	9.393675909513014	0.0
27	5.375570986430715E-4	0.0	0.0	9.523092781011332	0.0
28	5.375570986430715E-4	0.0	0.0	9.62415351555623	0.0
29	5.375570986430715E-4	0.0	0.0	9.731664935284845	0.0
30	5.375570986430715E-4	0.0	0.0	9.892394507779123	0.0
31	5.375570986430715E-4	0.0	0.0	9.996277417091896	0.0
32	5.375570986430715E-4	0.0	0.0	10.105804675940423	0.0
33	5.375570986430715E-4	0.0	0.0	10.221245062874022	0.0
34	5.375570986430715E-4	0.0	0.0	10.368670097176885	0.0
35	5.375570986430715E-4	0.0	0.0	10.504672043133581	0.0
36	5.375570986430715E-4	0.0	0.0	10.617290255299306	0.0
37	5.375570986430715E-4	0.0	0.0	10.72843018544376	0.0
38	5.375570986430715E-4	0.0	0.0	10.839435726313555	0.0
39	5.375570986430715E-4	0.0	0.0	10.97530328299559	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGAC	20	7.0316804E-4	45.000004	38
ATTCGCA	20	7.0316804E-4	45.000004	13
CGATTAG	40	6.8102963E-9	45.000004	1
TATGCGT	35	1.2111559E-7	45.000004	38
CCGTCTA	20	7.0316804E-4	45.000004	40
CGTTAAG	35	1.2111559E-7	45.000004	1
AGTGCGT	20	7.0316804E-4	45.000004	29
TACTCGA	20	7.0316804E-4	45.000004	40
CAGTACG	35	1.2111559E-7	45.000004	1
AATACGC	20	7.0316804E-4	45.000004	1
GATAGTC	35	1.2111559E-7	45.000004	9
TACCGAA	20	7.0316804E-4	45.000004	26
TATCACG	25	3.8894752E-5	45.0	1
TCGCATC	25	3.8894752E-5	45.0	10
TCCGATA	25	3.8894752E-5	45.0	12
GACGTAT	25	3.8894752E-5	45.0	37
CGTTGGA	25	3.8894752E-5	45.0	28
CGCATAG	45	3.8380676E-10	45.0	1
CGACAGT	25	3.8894752E-5	45.0	18
AATTCCG	25	3.8894752E-5	45.0	1
>>END_MODULE